8i9z - Revision history

OCA at 06:23, 19 July 2023

2023-07-19T06:23:24Z

←Older revision		Revision as of 06:23, 19 July 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8i9z]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I9Z FirstGlance]. <br>		<table><tr><td colspan='2'>[[8i9z]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I9Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i9z OCA], [https://pdbe.org/8i9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i9z RCSB], [https://www.ebi.ac.uk/pdbsum/8i9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i9z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i9z OCA], [https://pdbe.org/8i9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i9z RCSB], [https://www.ebi.ac.uk/pdbsum/8i9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i9z ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:02, 18 May 2023

2023-05-18T07:02:55Z

←Older revision		Revision as of 07:02, 18 May 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8i9z is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Spb4==
		+	<StructureSection load='8i9z' size='340' side='right'caption='[[8i9z]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8i9z]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I9Z FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i9z OCA], [https://pdbe.org/8i9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i9z RCSB], [https://www.ebi.ac.uk/pdbsum/8i9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i9z ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0RXZ3_CHATD G0RXZ3_CHATD]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ribosome biogenesis proceeds along a multifaceted pathway from the nucleolus to the cytoplasm that is extensively coupled to several quality control mechanisms. However, the mode by which 5S ribosomal RNA is incorporated into the developing pre-60S ribosome, which in humans links ribosome biogenesis to cell proliferation by surveillance by factors such as p53-MDM2, is poorly understood. Here, we report nine nucleolar pre-60S cryo-EM structures from Chaetomium thermophilum, one of which clarifies the mechanism of 5S RNP incorporation into the early pre-60S. Successive assembly states then represent how helicases Dbp10 and Spb4, and the Pumilio domain factor Puf6 act in series to surveil the gradual folding of the nearby 25S rRNA domain IV. Finally, the methyltransferase Spb1 methylates a universally conserved guanine nucleotide in the A-loop of the peptidyl transferase center, thereby licensing further maturation. Our findings provide insight into the hierarchical action of helicases in safeguarding rRNA tertiary structure folding and coupling to surveillance mechanisms that culminate in local RNA modification.

-	~~Authors~~:	+	Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis.,Lau B, Huang Z, Kellner N, Niu S, Berninghausen O, Beckmann R, Hurt E, Cheng J EMBO Rep. 2023 May 2:e56910. doi: 10.15252/embr.202356910. PMID:37129998<ref>PMID:37129998</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8i9z" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
		+	[[Category: Large Structures]]
		+	[[Category: Beckmann R]]
		+	[[Category: Cheng J]]
		+	[[Category: Huang Z]]
		+	[[Category: Hurt E]]
		+	[[Category: Lau B]]

OCA at 05:00, 7 April 2023

2023-04-07T05:00:36Z

←Older revision		Revision as of 05:00, 7 April 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8i9z is ON HOLD	+	The entry 8i9z is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:52, 29 March 2023

2023-03-29T04:52:48Z

←Older revision		Revision as of 04:52, 29 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8i9z is ON HOLD ~~until sometime in the future~~	+	The entry 8i9z is ON HOLD

	Authors:		Authors:

OCA at 04:38, 2 March 2023

2023-03-02T04:38:32Z

←Older revision		Revision as of 04:38, 2 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8i9z is ON HOLD	+	The entry 8i9z is ON HOLD until sometime in the future

	Authors:		Authors:

OCA: Protected "8i9z" [edit=sysop:move=sysop]

2023-02-15T09:01:33Z

Protected "8i9z" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:01, 15 February 2023

OCA: New page: '''Unreleased structure''' The entry 8i9z is ON HOLD Authors: Description: Category: Unreleased Structures

2023-02-15T09:01:32Z

New page: '''Unreleased structure''' The entry 8i9z is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8i9z is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]