OCA at 09:41, 21 June 2023

2023-06-21T09:41:10Z

←Older revision		Revision as of 09:41, 21 June 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8ilx is ~~ON HOLD until Paper~~ Publication	+	==mCherry-LaM3 complex==
		+	<StructureSection load='8ilx' size='340' side='right'caption='[[8ilx]], [[Resolution\|resolution]] 3.29Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ilx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anaplasma_marginale Anaplasma marginale] and [https://en.wikipedia.org/wiki/Camelus_bactrianus Camelus bactrianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ILX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ILX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CH6:{(4Z)-2-[(1S)-1-AMINO-3-(METHYLSULFANYL)PROPYL]-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CH6</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ilx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ilx OCA], [https://pdbe.org/8ilx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ilx RCSB], [https://www.ebi.ac.uk/pdbsum/8ilx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ilx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/X5DSL3_ANAMA X5DSL3_ANAMA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Red fluorescent proteins (RFPs) have broad applications in life science research, and the manipulation of RFPs using nanobodies can expand their potential uses. However, the structural information available for nanobodies that bind with RFPs is still insufficient. In this study, we cloned, expressed, purified, and crystallized complexes formed by mCherry with LaM1, LaM3, and LaM8. Then, we analyzed the biochemical properties of the complexes using mass spectrometry (MS), fluorescence-detected size exclusion chromatography (FSEC), isothermal titration calorimetry (ITC), and bio-layer interferometry (BLI) technology. We determined the crystal structure of mCherry-LaM1, mCherry-LaM3, and mCherry-LaM8, with resolutions of 2.05 A, 3.29 A, and 1.31 A, respectively. In this study, we systematically compared various parameters of several LaM series nanobodies, including LaM1, LaM3, and LaM8, with previously reported data on LaM2, LaM4, and LaM6, specifically examining their structural information. After designing multivalent tandem LaM1-LaM8 and LaM8-LaM4 nanobodies based on structural information, we characterized their properties, revealing their higher affinity and specificity to mCherry. Our research provides novel structural insights that could aid in understanding nanobodies targeting a specific target protein. This could provide a starting point for developing enhanced mCherry manipulation tools.

-	~~Authors:~~ Liang, H., Liu, R., Ding, Y.	+	Structural Insights into the Binding of Red Fluorescent Protein mCherry-Specific Nanobodies.,Liang H, Ma Z, Wang Z, Zhong P, Li R, Jiang H, Zong X, Zhong C, Liu X, Liu P, Liu J, Zhu H, Liu R, Ding Y Int J Mol Sci. 2023 Apr 9;24(8):6952. doi: 10.3390/ijms24086952. PMID:37108116<ref>PMID:37108116</ref>

-	~~Description: mCherry~~-~~LaM3 complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Liu, R~~]]	+	<div class="pdbe-citations 8ilx" style="background-color:#fffaf0;"></div>
-	[[Category: Ding, Y]]	+	== References ==
-	[[Category: Liang, H]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Anaplasma marginale]]
		+	[[Category: Camelus bactrianus]]
		+	[[Category: Large Structures]]
		+	[[Category: Ding Y]]
		+	[[Category: Liang H]]
		+	[[Category: Liu R]]

OCA at 04:55, 29 March 2023

2023-03-29T04:55:37Z

←Older revision		Revision as of 04:55, 29 March 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ilx is ON HOLD	+	The entry 8ilx is ON HOLD until Paper Publication

	Authors: Liang, H., Liu, R., Ding, Y.		Authors: Liang, H., Liu, R., Ding, Y.

OCA: Protected "8ilx" [edit=sysop:move=sysop]

2023-03-15T08:11:35Z

Protected "8ilx" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:11, 15 March 2023

OCA: New page: '''Unreleased structure''' The entry 8ilx is ON HOLD Authors: Liang, H., Liu, R., Ding, Y. Description: mCherry-LaM3 complex Category: Unreleased Structures Category: Liu, R [[...

2023-03-15T08:11:32Z

New page: '''Unreleased structure''' The entry 8ilx is ON HOLD Authors: Liang, H., Liu, R., Ding, Y. Description: mCherry-LaM3 complex Category: Unreleased Structures Category: Liu, R [[...

New page

'''Unreleased structure'''

The entry 8ilx is ON HOLD

Authors: Liang, H., Liu, R., Ding, Y.

Description: mCherry-LaM3 complex
[[Category: Unreleased Structures]]
[[Category: Liu, R]]
[[Category: Ding, Y]]
[[Category: Liang, H]]

8ilx - Revision history

OCA at 09:41, 21 June 2023

OCA at 04:55, 29 March 2023

OCA: Protected "8ilx" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8ilx is ON HOLD Authors: Liang, H., Liu, R., Ding, Y. Description: mCherry-LaM3 complex Category: Unreleased Structures Category: Liu, R [[...