8iq6 - Revision history

OCA at 11:42, 1 February 2024

2024-02-01T11:42:33Z

←Older revision		Revision as of 11:42, 1 February 2024
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-	'''Unreleased structure'''

-	The entry ~~8iq6~~ is ~~ON HOLD~~	+	==Cryo-EM structure of Latanoprost-bound prostaglandin-F2-alpha receptor-miniGq-Nb35 complex==
		+	<StructureSection load='8iq6' size='340' side='right'caption='[[8iq6]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8iq6]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IQ6 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7WT:Z-7-[(1R,2R,3R,5S)-3,5-bis(oxidanyl)-2-[(3R)-3-oxidanyl-5-phenyl-pentyl]cyclopentyl]hept-5-enoic+acid'>7WT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8iq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8iq6 OCA], [https://pdbe.org/8iq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8iq6 RCSB], [https://www.ebi.ac.uk/pdbsum/8iq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8iq6 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/GNAS2_HUMAN GNAS2_HUMAN] Pseudopseudohypoparathyroidism;Pseudohypoparathyroidism type 1A;Progressive osseous heteroplasia;Polyostotic fibrous dysplasia;Monostotic fibrous dysplasia;Pseudohypoparathyroidism type 1C;Pseudohypoparathyroidism type 1B;McCune-Albright syndrome. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. Most affected individuals have defects in methylation of the gene. In some cases microdeletions involving the STX16 appear to cause loss of methylation at exon A/B of GNAS, resulting in PHP1B. Paternal uniparental isodisomy have also been observed. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GNAS2_HUMAN GNAS2_HUMAN] Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed:17110384). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed:26206488, PubMed:8702665). GNAS functions downstream of several GPCRs, including beta-adrenergic receptors (PubMed:21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed:12391161).<ref>PMID:12391161</ref> <ref>PMID:17110384</ref> <ref>PMID:21488135</ref> <ref>PMID:26206488</ref> <ref>PMID:8702665</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Prostaglandins and their receptors regulate various physiological processes. Carboprost, an analog of prostaglandin F(2alpha) and an agonist for the prostaglandin F2-alpha receptor (FP receptor), is clinically used to treat postpartum hemorrhage (PPH). However, off-target activation of closely related receptors such as the prostaglandin E receptor subtype EP3 (EP3 receptor) by carboprost results in side effects and limits the clinical application. Meanwhile, the FP receptor selective agonist latanoprost is not suitable to treat PPH due to its poor solubility and fast clearance. Here, we present two cryo-EM structures of the FP receptor bound to carboprost and latanoprost-FA (the free acid form of latanoprost) at 2.7 A and 3.2 A resolution, respectively. The structures reveal the molecular mechanism of FP receptor selectivity for both endogenous prostaglandins and clinical drugs, as well as the molecular mechanism of G protein coupling preference by the prostaglandin receptors. The structural information may guide the development of better prostaglandin drugs.

-	~~Authors:~~ Lv, X., Gao, K., Nie, J., Zhang, X., Zhang, S., Ren, Y., Li, Q., Huang, J., Liu, L., Zhang, X~~., Sun, X.~~, Zhang, W., Liu, X.	+	Structures of human prostaglandin F(2alpha) receptor reveal the mechanism of ligand and G protein selectivity.,Lv X, Gao K, Nie J, Zhang X, Zhang S, Ren Y, Sun X, Li Q, Huang J, Liu L, Zhang X, Zhang W, Liu X Nat Commun. 2023 Dec 8;14(1):8136. doi: 10.1038/s41467-023-43922-8. PMID:38065938<ref>PMID:38065938</ref>

-	~~Description: Cryo-EM structure~~ of ~~Latanoprost~~-~~bound prostaglandin~~-~~F2-alpha receptor-miniGq-Nb35 complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Liu, X~~]]	+	<div class="pdbe-citations 8iq6" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Lv, X~~]]	+	== References ==
-	[[Category: ~~Liu, L~~]]	+	<references/>
-	[[Category: Li, Q]]	+	__TOC__
-	[[Category: ~~Ren, Y~~]]	+	</StructureSection>
-	[[Category: ~~Zhang, S~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Huang,~~ J]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sun, X~~]]	+	[[Category: Vicugna pacos]]
-	[[Category: ~~Zhang, W~~]]	+	[[Category: Gao K]]
-	[[Category: Zhang~~, X~~]]	+	[[Category: Huang J]]
-	[[Category: ~~Gao, K~~]]	+	[[Category: Li Q]]
-	[[Category: ~~Nie, J~~]]	+	[[Category: Liu L]]
		+	[[Category: Liu X]]
		+	[[Category: Lv X]]
		+	[[Category: Nie J]]
		+	[[Category: Ren Y]]
		+	[[Category: Sun X]]
		+	[[Category: Zhang S]]
		+	[[Category: Zhang W]]
		+	[[Category: Zhang X]]

OCA at 10:07, 24 January 2024

2024-01-24T10:07:41Z

←Older revision		Revision as of 10:07, 24 January 2024
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8iq6 is ON HOLD ~~until Paper Publication~~	+	The entry 8iq6 is ON HOLD

	Authors: Lv, X., Gao, K., Nie, J., Zhang, X., Zhang, S., Ren, Y., Li, Q., Huang, J., Liu, L., Zhang, X., Sun, X., Zhang, W., Liu, X.		Authors: Lv, X., Gao, K., Nie, J., Zhang, X., Zhang, S., Ren, Y., Li, Q., Huang, J., Liu, L., Zhang, X., Sun, X., Zhang, W., Liu, X.
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	Description: Cryo-EM structure of Latanoprost-bound prostaglandin-F2-alpha receptor-miniGq-Nb35 complex		Description: Cryo-EM structure of Latanoprost-bound prostaglandin-F2-alpha receptor-miniGq-Nb35 complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Liu, L]]
-	[[Category: Ren, Y]]
-	[[Category: Zhang, S]]
-	[[Category: Zhang, W]]
-	[[Category: Zhang, X]]
	[[Category: Liu, X]]		[[Category: Liu, X]]
	[[Category: Lv, X]]		[[Category: Lv, X]]
		+	[[Category: Liu, L]]
	[[Category: Li, Q]]		[[Category: Li, Q]]
		+	[[Category: Ren, Y]]
		+	[[Category: Zhang, S]]
	[[Category: Huang, J]]		[[Category: Huang, J]]
	[[Category: Sun, X]]		[[Category: Sun, X]]
		+	[[Category: Zhang, W]]
		+	[[Category: Zhang, X]]
	[[Category: Gao, K]]		[[Category: Gao, K]]
	[[Category: Nie, J]]		[[Category: Nie, J]]

OCA at 05:02, 7 April 2023

2023-04-07T05:02:25Z

←Older revision		Revision as of 05:02, 7 April 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8iq6 is ON HOLD	+	The entry 8iq6 is ON HOLD until Paper Publication

	Authors: Lv, X., Gao, K., Nie, J., Zhang, X., Zhang, S., Ren, Y., Li, Q., Huang, J., Liu, L., Zhang, X., Sun, X., Zhang, W., Liu, X.		Authors: Lv, X., Gao, K., Nie, J., Zhang, X., Zhang, S., Ren, Y., Li, Q., Huang, J., Liu, L., Zhang, X., Sun, X., Zhang, W., Liu, X.

-	Description: Cryo-EM structure of ~~Carboprost~~-bound prostaglandin-F2-alpha receptor-miniGq-Nb35 complex	+	Description: Cryo-EM structure of Latanoprost-bound prostaglandin-F2-alpha receptor-miniGq-Nb35 complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Liu, L]]		[[Category: Liu, L]]

OCA: Protected "8iq6" [edit=sysop:move=sysop]

2023-03-22T05:55:34Z

Protected "8iq6" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:55, 22 March 2023

OCA: New page: '''Unreleased structure''' The entry 8iq6 is ON HOLD Authors: Lv, X., Gao, K., Nie, J., Zhang, X., Zhang, S., Ren, Y., Li, Q., Huang, J., Liu, L., Zhang, X., Sun, X., Zhang, W., Liu, X....

2023-03-22T05:55:33Z

New page: '''Unreleased structure''' The entry 8iq6 is ON HOLD Authors: Lv, X., Gao, K., Nie, J., Zhang, X., Zhang, S., Ren, Y., Li, Q., Huang, J., Liu, L., Zhang, X., Sun, X., Zhang, W., Liu, X....

New page

'''Unreleased structure'''

The entry 8iq6 is ON HOLD

Authors: Lv, X., Gao, K., Nie, J., Zhang, X., Zhang, S., Ren, Y., Li, Q., Huang, J., Liu, L., Zhang, X., Sun, X., Zhang, W., Liu, X.

Description: Cryo-EM structure of Carboprost-bound prostaglandin-F2-alpha receptor-miniGq-Nb35 complex
[[Category: Unreleased Structures]]
[[Category: Liu, L]]
[[Category: Ren, Y]]
[[Category: Zhang, S]]
[[Category: Zhang, W]]
[[Category: Zhang, X]]
[[Category: Liu, X]]
[[Category: Lv, X]]
[[Category: Li, Q]]
[[Category: Huang, J]]
[[Category: Sun, X]]
[[Category: Gao, K]]
[[Category: Nie, J]]