OCA at 05:54, 23 August 2023

2023-08-23T05:54:45Z

←Older revision		Revision as of 05:54, 23 August 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8iql is ~~ON HOLD until Paper~~ Publication	+	==Structural basis of the specificity and interaction mechanism of Bmf binding to pro-survival proteins==
		+	<StructureSection load='8iql' size='340' side='right'caption='[[8iql]], [[Resolution\|resolution]] 2.96Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8iql]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IQL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IQL FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9577Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8iql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8iql OCA], [https://pdbe.org/8iql PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8iql RCSB], [https://www.ebi.ac.uk/pdbsum/8iql PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8iql ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BMF_HUMAN BMF_HUMAN] May play a role in apoptosis. Isoform 1 seems to be the main initiator.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The apoptotic pathway is regulated by protein-protein interactions between members of the Bcl-2 family. Pro-survival Bcl-2 family proteins act as cell guardians and protect cells against death. Selective binding and neutralization of BH3-only proteins with pro-survival Bcl-2 family proteins is critical for initiating apoptosis. In this study, the binding assay shows that the BH3 peptide derived from the BH3-only protein Bmf has a high affinity for the pro-survival proteins Bcl-2 and Bcl-xL, but a much lower affinity for Mcl-1. The complex structures of Bmf BH3 with Bcl-2, Bcl-xL and Mcl-1 reveal that the alpha-helical Bmf BH3 accommodates into the canonical groove of these pro-survival proteins, but the conformational changes and some interactions are different among the three complexes. Bmf BH3 forms conserved hydrophobic and salt bridge interactions with Bcl-2 and Bcl-xL, and also establishes several hydrogen bonds to support their binding. However, the highly conserved Asp-Arg salt bridge is not formed in the Mcl-1/Bmf BH3 complex, and few hydrogen bonds are observed. Furthermore, mutational analysis shows that substitutions of less-conserved residues in the alpha2-alpha3 region of these pro-survival Bcl-2 family proteins, as well as the highly conserved Arg, lead to significant changes in their binding affinity to Bmf BH3, while substitutions of less-conserved residues in Bmf BH3 have a more dramatic effect on its affinity to Mcl-1. This study provides structural insight into the specificity and interaction mechanism of Bmf BH3 binding to pro-survival Bcl-2 family proteins, and helps guide the design of BH3 mimics targeting pro-survival Bcl-2 family proteins.

-	~~Authors: Wang~~, H., Guo, M., Wei, H., Chen, Y.	+	Structural basis of the specificity and interaction mechanism of Bmf binding to pro-survival Bcl-2 family proteins.,Wang H, Guo M, Wei H, Chen Y Comput Struct Biotechnol J. 2023 Jul 21;21:3760-3767. doi: , 10.1016/j.csbj.2023.07.017. eCollection 2023. PMID:37560128<ref>PMID:37560128</ref>

-	~~Description: Structural basis~~ of the ~~specificity and interaction mechanism~~ of ~~Bmf binding to pro~~-~~survival proteins~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Guo, M~~]]	+	<div class="pdbe-citations 8iql" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Wei, H~~]]	+	== References ==
-	[[Category: Wang, H]]	+	<references/>
-	[[Category: ~~Chen, Y~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Chen Y]]
		+	[[Category: Guo M]]
		+	[[Category: Wang H]]
		+	[[Category: Wei H]]

OCA at 05:04, 10 May 2023

2023-05-10T05:04:16Z

←Older revision		Revision as of 05:04, 10 May 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8iql is ON HOLD	+	The entry 8iql is ON HOLD until Paper Publication

	Authors: Wang, H., Guo, M., Wei, H., Chen, Y.		Authors: Wang, H., Guo, M., Wei, H., Chen, Y.
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	[[Category: Guo, M]]		[[Category: Guo, M]]
	[[Category: Wei, H]]		[[Category: Wei, H]]
-	[[Category: Chen, Y]]
	[[Category: Wang, H]]		[[Category: Wang, H]]
		+	[[Category: Chen, Y]]

OCA: Protected "8iql" [edit=sysop:move=sysop]

2023-03-22T05:56:15Z

Protected "8iql" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:56, 22 March 2023

OCA: New page: '''Unreleased structure''' The entry 8iql is ON HOLD Authors: Wang, H., Guo, M., Wei, H., Chen, Y. Description: Structural basis of the specificity and interaction mechanism of Bmf bin...

2023-03-22T05:56:12Z

New page: '''Unreleased structure''' The entry 8iql is ON HOLD Authors: Wang, H., Guo, M., Wei, H., Chen, Y. Description: Structural basis of the specificity and interaction mechanism of Bmf bin...

New page

'''Unreleased structure'''

The entry 8iql is ON HOLD

Authors: Wang, H., Guo, M., Wei, H., Chen, Y.

Description: Structural basis of the specificity and interaction mechanism of Bmf binding to pro-survival proteins
[[Category: Unreleased Structures]]
[[Category: Guo, M]]
[[Category: Wei, H]]
[[Category: Chen, Y]]
[[Category: Wang, H]]

8iql - Revision history

OCA at 05:54, 23 August 2023

OCA at 05:04, 10 May 2023

OCA: Protected "8iql" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8iql is ON HOLD Authors: Wang, H., Guo, M., Wei, H., Chen, Y. Description: Structural basis of the specificity and interaction mechanism of Bmf bin...