OCA at 14:08, 18 October 2023

2023-10-18T14:08:34Z

←Older revision		Revision as of 14:08, 18 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8iss is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of wild-type human tRNA Splicing Endonuclease Complex bound to pre-tRNA-ARG at 3.19 A resolution==
		+	<StructureSection load='8iss' size='340' side='right'caption='[[8iss]], [[Resolution\|resolution]] 3.19Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8iss]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ISS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ISS FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.19Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8iss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8iss OCA], [https://pdbe.org/8iss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8iss RCSB], [https://www.ebi.ac.uk/pdbsum/8iss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8iss ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SEN15_HUMAN SEN15_HUMAN] Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3' end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.<ref>PMID:15109492</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Removal of introns from transfer RNA precursors (pre-tRNAs) occurs in all living organisms. This is a vital phase in the maturation and functionality of tRNA. Here we present a 3.2 A-resolution cryo-EM structure of an active human tRNA splicing endonuclease complex bound to an intron-containing pre-tRNA. TSEN54, along with the unique regions of TSEN34 and TSEN2, cooperatively recognizes the mature body of pre-tRNA and guides the anticodon-intron stem to the correct position for splicing. We capture the moment when the endonucleases are poised for cleavage, illuminating the molecular mechanism for both 3' and 5' cleavage reactions. Two insertion loops from TSEN54 and TSEN2 cover the 3' and 5' splice sites, respectively, trapping the scissile phosphate in the center of the catalytic triad of residues. Our findings reveal the molecular mechanism for eukaryotic pre-tRNA recognition and cleavage, as well as the evolutionary relationship between archaeal and eukaryotic TSENs.

-	~~Authors~~:	+	Recognition and cleavage mechanism of intron-containing pre-tRNA by human TSEN endonuclease complex.,Yuan L, Han Y, Zhao J, Zhang Y, Sun Y Nat Commun. 2023 Sep 28;14(1):6071. doi: 10.1038/s41467-023-41845-y. PMID:37770519<ref>PMID:37770519</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8iss" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Han Y]]
		+	[[Category: Sun Y]]
		+	[[Category: Yuan L]]
		+	[[Category: Zhang Y]]

OCA at 10:43, 4 October 2023

2023-10-04T10:43:43Z

←Older revision		Revision as of 10:43, 4 October 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8iss is ON HOLD	+	The entry 8iss is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 11:50, 20 September 2023

2023-09-20T11:50:44Z

←Older revision		Revision as of 11:50, 20 September 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8iss is ON HOLD ~~until Paper Publication~~	+	The entry 8iss is ON HOLD

	Authors:		Authors:

OCA at 05:25, 3 May 2023

2023-05-03T05:25:29Z

←Older revision		Revision as of 05:25, 3 May 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8iss is ON HOLD	+	The entry 8iss is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8iss" [edit=sysop:move=sysop]

2023-03-29T05:00:46Z

Protected "8iss" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:00, 29 March 2023

OCA: New page: '''Unreleased structure''' The entry 8iss is ON HOLD Authors: Description: Category: Unreleased Structures

2023-03-29T05:00:45Z

New page: '''Unreleased structure''' The entry 8iss is ON HOLD Authors: Description: Category: Unreleased Structures

New page