OCA at 11:42, 1 February 2024

2024-02-01T11:42:36Z

←Older revision		Revision as of 11:42, 1 February 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8itr is ~~ON HOLD~~	+	==Crystal structure of lysophosphatidylcholine in complex with human serum albumin==
		+	<StructureSection load='8itr' size='340' side='right'caption='[[8itr]], [[Resolution\|resolution]] 2.44Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8itr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ITR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ITR FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.44Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LPC:[1-MYRISTOYL-GLYCEROL-3-YL]PHOSPHONYLCHOLINE'>LPC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8itr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8itr OCA], [https://pdbe.org/8itr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8itr RCSB], [https://www.ebi.ac.uk/pdbsum/8itr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8itr ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:[https://omim.org/entry/103600 103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.<ref>PMID:8048949</ref> <ref>PMID:7852505</ref> <ref>PMID:9329347</ref> <ref>PMID:9589637</ref>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.<ref>PMID:19021548</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lysophospholipids (lysoPLs) are crucial metabolites involved in various physiological and pathological cellular processes. Understanding their binding interactions, particularly with human serum albumin (HSA), is essential due to their role in regulating lysoPLs-induced cytotoxicity. However, the precise mechanism of lysoPLs binding to HSA remains elusive. In this study, we employed fluorescence quenching and optical interferometry assays to demonstrate direct binding between lysophosphatidylcholine (LPC) and HSA (K(D) = 25 muM). Furthermore, we determined crystal structures of HSA in complex with LPC, both in the absence and the presence of the endogenous fatty acid myristate (14:0). The crystal structure of binary HSA:LPC revealed that six LPC molecules are bound to HSA at the primary fatty acid binding sites. Interestingly, the ternary HSA:Myr:LPC structure demonstrated the continued binding of three LPC molecules to HSA at binding sites 1, 3, and 5 in the presence of myristate. These findings support HSA's role as a carrier protein for lysoPLs in blood plasma and provide valuable insights into the structural basis of their binding mechanisms.

-	~~Authors: Wang~~, Y., ~~Jiang~~, L~~.G.~~, ~~Huang~~, M.D.	+	Crystal structures of human serum albumin in complex with lysophosphatidylcholine.,Wang Y, Luo Z, Morelli X, Xu P, Jiang L, Shi X, Huang M Biophys J. 2023 Nov 7;122(21):4135-4143. doi: 10.1016/j.bpj.2023.09.007. Epub , 2023 Sep 19. PMID:37731243<ref>PMID:37731243</ref>

-	~~Description: Crystal structure~~ of ~~lysophosphatidylcholine in complex with human serum albumin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Huang~~, M.D~~]]	+	<div class="pdbe-citations 8itr" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Wang, Y~~]]	+	== References ==
-	[[Category: ~~Jiang, L.G~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Huang MD]]
		+	[[Category: Jiang LG]]
		+	[[Category: Wang Y]]

OCA: Protected "8itr" [edit=sysop:move=sysop]

2023-03-29T05:02:20Z

Protected "8itr" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:02, 29 March 2023

OCA: New page: '''Unreleased structure''' The entry 8itr is ON HOLD Authors: Wang, Y., Jiang, L.G., Huang, M.D. Description: Crystal structure of lysophosphatidylcholine in complex with human serum a...

2023-03-29T05:02:19Z

New page: '''Unreleased structure''' The entry 8itr is ON HOLD Authors: Wang, Y., Jiang, L.G., Huang, M.D. Description: Crystal structure of lysophosphatidylcholine in complex with human serum a...

New page

'''Unreleased structure'''

The entry 8itr is ON HOLD

Authors: Wang, Y., Jiang, L.G., Huang, M.D.

Description: Crystal structure of lysophosphatidylcholine in complex with human serum albumin
[[Category: Unreleased Structures]]
[[Category: Huang, M.D]]
[[Category: Wang, Y]]
[[Category: Jiang, L.G]]

8itr - Revision history

OCA at 11:42, 1 February 2024

OCA: Protected "8itr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8itr is ON HOLD Authors: Wang, Y., Jiang, L.G., Huang, M.D. Description: Crystal structure of lysophosphatidylcholine in complex with human serum a...