OCA at 14:45, 20 September 2023

2023-09-20T14:45:30Z

←Older revision		Revision as of 14:45, 20 September 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8j3o is ~~ON HOLD until 2025~~-04-17	+	==Formate dehydrogenase wild-type enzyme from Candida dubliniensis complexed with NADH==
		+	<StructureSection load='8j3o' size='340' side='right'caption='[[8j3o]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8j3o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_dubliniensis_CD36 Candida dubliniensis CD36]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8J3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8J3O FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j3o OCA], [https://pdbe.org/8j3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j3o RCSB], [https://www.ebi.ac.uk/pdbsum/8j3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j3o ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B9WHT3_CANDC B9WHT3_CANDC] Catalyzes the NAD(+)-dependent oxidation of formate to carbon dioxide. Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms. Has a role in the detoxification of exogenous formate in non-methylotrophic organisms.[HAMAP-Rule:MF_03210]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH) constitute major hydrogen donors for oxidative/reductive bio-transformations. NAD(P)H regeneration systems coupled with formate dehydrogenases (FDHs) represent a dreamful method. However, most of the native FDHs are NAD(+) -dependent and suffer from insufficient reactivity compared to other enzymatic tools, such as glucose dehydrogenase. An efficient and competitive NADP(+) -utilizing FDH necessitates the availability and robustness of NADPH regeneration systems. Herein, we report the engineering of a new FDH from Candida dubliniensis (CdFDH), which showed no strict NAD(+) preference by a structure-guided rational/semi-rational design. A combinatorial mutant CdFDH-M4 (D197Q/Y198R/Q199N/A372S/K371T/triangle upQ375/K167R/H16L/K159R) exhibited 75-fold intensification of catalytic efficiency (k(cat) /K(m) ). Moreover, CdFDH-M4 has been successfully employed in diverse asymmetric oxidative/reductive processes with cofactor total turnover numbers (TTNs) ranging from 135 to 986, making it potentially useful for NADPH-required biocatalytic transformations.

-	~~Authors: Ma~~, W~~., Zheng, Y.C.~~, Geng, Q., Chen, C.	+	Engineering a Formate Dehydrogenase for NADPH Regeneration.,Ma W, Geng Q, Chen C, Zheng YC, Yu HL, Xu JH Chembiochem. 2023 Jul 16:e202300390. doi: 10.1002/cbic.202300390. PMID:37455264<ref>PMID:37455264</ref>

-	~~Description: Formate dehydrogenase wild~~-~~type enzyme from~~ Candida dubliniensis ~~complexed with NADH~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Ma, W~~]]	+	<div class="pdbe-citations 8j3o" style="background-color:#fffaf0;"></div>
-	[[Category: Geng, Q]]	+	== References ==
-	[[Category: ~~Chen, C~~]]	+	<references/>
-	[[Category: Zheng~~, Y.C~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Candida dubliniensis CD36]]
		+	[[Category: Large Structures]]
		+	[[Category: Chen C]]
		+	[[Category: Geng Q]]
		+	[[Category: Ma W]]
		+	[[Category: Zheng YC]]

OCA at 06:18, 14 June 2023

2023-06-14T06:18:41Z

←Older revision		Revision as of 06:18, 14 June 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8j3o is ON HOLD	+	The entry 8j3o is ON HOLD until 2025-04-17

	Authors: Ma, W., Zheng, Y.C., Geng, Q., Chen, C.		Authors: Ma, W., Zheng, Y.C., Geng, Q., Chen, C.

OCA at 05:29, 3 May 2023

2023-05-03T05:29:16Z

←Older revision		Revision as of 05:29, 3 May 2023
Line 3:		Line 3:
	The entry 8j3o is ON HOLD		The entry 8j3o is ON HOLD

-	Authors: Ma, W., Zheng, Y.-C., Geng, Q., Chen, C.	+	Authors: Ma, W., Zheng, Y.C., Geng, Q., Chen, C.

	Description: Formate dehydrogenase wild-type enzyme from Candida dubliniensis complexed with NADH		Description: Formate dehydrogenase wild-type enzyme from Candida dubliniensis complexed with NADH
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Ma, W]]		[[Category: Ma, W]]
-	[[Category: Zheng, Y.-C]]
	[[Category: Geng, Q]]		[[Category: Geng, Q]]
	[[Category: Chen, C]]		[[Category: Chen, C]]
		+	[[Category: Zheng, Y.C]]

OCA: Protected "8j3o" [edit=sysop:move=sysop]

2023-04-26T15:04:22Z

Protected "8j3o" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:04, 26 April 2023

OCA: New page: '''Unreleased structure''' The entry 8j3o is ON HOLD Authors: Ma, W., Zheng, Y.-C., Geng, Q., Chen, C. Description: Formate dehydrogenase wild-type enzyme from Candida dubliniensis com...

2023-04-26T15:04:21Z

New page: '''Unreleased structure''' The entry 8j3o is ON HOLD Authors: Ma, W., Zheng, Y.-C., Geng, Q., Chen, C. Description: Formate dehydrogenase wild-type enzyme from Candida dubliniensis com...

New page

'''Unreleased structure'''

The entry 8j3o is ON HOLD

Authors: Ma, W., Zheng, Y.-C., Geng, Q., Chen, C.

Description: Formate dehydrogenase wild-type enzyme from Candida dubliniensis complexed with NADH
[[Category: Unreleased Structures]]
[[Category: Ma, W]]
[[Category: Zheng, Y.-C]]
[[Category: Geng, Q]]
[[Category: Chen, C]]

8j3o - Revision history

OCA at 14:45, 20 September 2023

OCA at 06:18, 14 June 2023

OCA at 05:29, 3 May 2023

OCA: Protected "8j3o" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8j3o is ON HOLD Authors: Ma, W., Zheng, Y.-C., Geng, Q., Chen, C. Description: Formate dehydrogenase wild-type enzyme from Candida dubliniensis com...