OCA at 14:46, 20 September 2023

2023-09-20T14:46:01Z

←Older revision		Revision as of 14:46, 20 September 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8j7v is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of hZnT7-Fab complex in zinc-unbound state, determined in heterogeneous conformations- one subunit in an inward-facing and the other in an outward-facing conformation==
		+	<StructureSection load='8j7v' size='340' side='right'caption='[[8j7v]], [[Resolution\|resolution]] 2.79Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8j7v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8J7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8J7V FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.79Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j7v OCA], [https://pdbe.org/8j7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j7v RCSB], [https://www.ebi.ac.uk/pdbsum/8j7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j7v ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ZNT7_HUMAN ZNT7_HUMAN] Zinc ion transporter mediating zinc entry from the cytosol into the lumen of organelles along the secretory pathway (PubMed:15525635, PubMed:15994300). By contributing to zinc ion homeostasis within the early secretory pathway, regulates the activation and folding of enzymes like alkaline phosphatases (PubMed:15525635, PubMed:15994300).<ref>PMID:15525635</ref> <ref>PMID:15994300</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Zinc ions (Zn(2+)) are vital to most cells, with the intracellular concentrations of Zn(2+) being tightly regulated by multiple zinc transporters located at the plasma and organelle membranes. We herein present the 2.2-3.1 A-resolution cryo-EM structures of a Golgi-localized human Zn(2+)/H(+) antiporter ZnT7 (hZnT7) in Zn(2+)-bound and unbound forms. Cryo-EM analyses show that hZnT7 exists as a dimer via tight interactions in both the cytosolic and transmembrane (TM) domains of two protomers, each of which contains a single Zn(2+)-binding site in its TM domain. hZnT7 undergoes a TM-helix rearrangement to create a negatively charged cytosolic cavity for Zn(2+) entry in the inward-facing conformation and widens the luminal cavity for Zn(2+) release in the outward-facing conformation. An exceptionally long cytosolic histidine-rich loop characteristic of hZnT7 binds two Zn(2+) ions, seemingly facilitating Zn(2+) recruitment to the TM metal transport pathway. These structures permit mechanisms of hZnT7-mediated Zn(2+) uptake into the Golgi to be proposed.

-	~~Authors~~:	+	Cryo-EM structures of human zinc transporter ZnT7 reveal the mechanism of Zn(2+) uptake into the Golgi apparatus.,Bui HB, Watanabe S, Nomura N, Liu K, Uemura T, Inoue M, Tsutsumi A, Fujita H, Kinoshita K, Kato Y, Iwata S, Kikkawa M, Inaba K Nat Commun. 2023 Aug 8;14(1):4770. doi: 10.1038/s41467-023-40521-5. PMID:37553324<ref>PMID:37553324</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8j7v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
		+	[[Category: Han BB]]
		+	[[Category: Inaba K]]
		+	[[Category: Watanabe S]]

OCA at 08:00, 21 June 2023

2023-06-21T08:00:49Z

←Older revision		Revision as of 08:00, 21 June 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8j7v is ON HOLD	+	The entry 8j7v is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8j7v" [edit=sysop:move=sysop]

2023-05-03T05:37:20Z

Protected "8j7v" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:37, 3 May 2023

OCA: New page: '''Unreleased structure''' The entry 8j7v is ON HOLD Authors: Description: Category: Unreleased Structures

2023-05-03T05:37:19Z

New page: '''Unreleased structure''' The entry 8j7v is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8j7v is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8j7v - Revision history

OCA at 14:46, 20 September 2023

OCA at 08:00, 21 June 2023

OCA: Protected "8j7v" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8j7v is ON HOLD Authors: Description: Category: Unreleased Structures