8pjh - Revision history

OCA at 08:23, 7 February 2024

2024-02-07T08:23:21Z

←Older revision		Revision as of 08:23, 7 February 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/INS_BALPH INS_BALPH] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.		[https://www.uniprot.org/uniprot/INS_BALPH INS_BALPH] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Since the first administration of insulin to a person with diabetes in 1922, scientific contributions from academia and industry have improved insulin therapy and access. The pharmaceutical need for insulin is now more than 40 tons annually, half of which is produced by recombinant secretory expression in Saccharomyces cerevisiae. We discuss how, in this yeast species, adaptation of insulin precursors by removable structural elements is pivotal for efficient secretory expression. The technologies reviewed have been implemented at industrial scale and are seminal for the supply of human insulin and insulin analogues to people with diabetes now and in the future. Engineering of a target protein with removable structural elements may provide a general approach to yield optimisation.
-
-	Molecular engineering of insulin for recombinant expression in yeast.,Kjeldsen T, Andersen AS, Hubalek F, Johansson E, Kreiner FF, Schluckebier G, Kurtzhals P Trends Biotechnol. 2023 Oct 24:S0167-7799(23)00286-X. doi: , 10.1016/j.tibtech.2023.09.012. PMID:37880066<ref>PMID:37880066</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8pjh" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:03, 8 November 2023

2023-11-08T12:03:33Z

←Older revision		Revision as of 12:03, 8 November 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8pjh is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of human insulin desB30 precursor with an Aspartate-Glycine-Lysine C-peptide in dimer (T2) conformation==
		+	<StructureSection load='8pjh' size='340' side='right'caption='[[8pjh]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8pjh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PJH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PJH FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pjh OCA], [https://pdbe.org/8pjh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pjh RCSB], [https://www.ebi.ac.uk/pdbsum/8pjh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pjh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/INS_BALPH INS_BALPH] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Since the first administration of insulin to a person with diabetes in 1922, scientific contributions from academia and industry have improved insulin therapy and access. The pharmaceutical need for insulin is now more than 40 tons annually, half of which is produced by recombinant secretory expression in Saccharomyces cerevisiae. We discuss how, in this yeast species, adaptation of insulin precursors by removable structural elements is pivotal for efficient secretory expression. The technologies reviewed have been implemented at industrial scale and are seminal for the supply of human insulin and insulin analogues to people with diabetes now and in the future. Engineering of a target protein with removable structural elements may provide a general approach to yield optimisation.

-	~~Authors: Schluckebier, G~~., ~~Johansson~~, E.	+	Molecular engineering of insulin for recombinant expression in yeast.,Kjeldsen T, Andersen AS, Hubalek F, Johansson E, Kreiner FF, Schluckebier G, Kurtzhals P Trends Biotechnol. 2023 Oct 24:S0167-7799(23)00286-X. doi: , 10.1016/j.tibtech.2023.09.012. PMID:37880066<ref>PMID:37880066</ref>

-	~~Description: Crystal structure~~ of ~~human insulin desB30 precursor with an Aspartate~~-~~Glycine~~-~~Lysine C-peptide in dimer (T2) conformation~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Johansson, E]]	+	<div class="pdbe-citations 8pjh" style="background-color:#fffaf0;"></div>
-	[[Category: Schluckebier, G]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Johansson E]]
		+	[[Category: Schluckebier G]]

OCA: Protected "8pjh" [edit=sysop:move=sysop]

2023-07-05T04:12:27Z

Protected "8pjh" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:12, 5 July 2023

OCA: New page: '''Unreleased structure''' The entry 8pjh is ON HOLD until Paper Publication Authors: Schluckebier, G., Johansson, E. Description: Crystal structure of human insulin desB30 precursor w...

2023-07-05T04:12:23Z

New page: '''Unreleased structure''' The entry 8pjh is ON HOLD until Paper Publication Authors: Schluckebier, G., Johansson, E. Description: Crystal structure of human insulin desB30 precursor w...

New page

'''Unreleased structure'''

The entry 8pjh is ON HOLD until Paper Publication

Authors: Schluckebier, G., Johansson, E.

Description: Crystal structure of human insulin desB30 precursor with an Aspartate-Glycine-Lysine C-peptide in dimer (T2) conformation
[[Category: Unreleased Structures]]
[[Category: Johansson, E]]
[[Category: Schluckebier, G]]