OCA at 11:29, 24 January 2024

2024-01-24T11:29:31Z

←Older revision		Revision as of 11:29, 24 January 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8r37 is ~~ON HOLD until Paper~~ Publication	+	==Klebsiella pneumoniae fosfomycin-resistance protein (FosAKP)==
		+	<StructureSection load='8r37' size='340' side='right'caption='[[8r37]], [[Resolution\|resolution]] 1.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8r37]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8R37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8R37 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.48Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FCN:FOSFOMYCIN'>FCN</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8r37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8r37 OCA], [https://pdbe.org/8r37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8r37 RCSB], [https://www.ebi.ac.uk/pdbsum/8r37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8r37 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A086IRG1_KLEPN A0A086IRG1_KLEPN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fosfomycin-resistance proteins (FosAs) are dimeric metal-dependent glutathione transferases that conjugate the antibiotic fosfomycin (Fos) to the tripeptide glutathione (gamma-Glu-Cys-Gly, GSH), rendering it inactive. In the present study, we reported a comparative analysis of the functional features of two FosAs from Pseudomonas aeruginosa (FosAPA) and Klebsiella pneumoniae (FosAKP). The coding sequences of the enzymes were cloned into a T7 expression vector, and soluble active enzymes were expressed in E. coli. FosAKP displayed higher activity and was selected for further studies. The crystal structure of the dimeric FosAKP was determined via X-ray crystallography at 1.48 A resolution. Fos and tartrate (Tar) were found bound in the active site of the first and second molecules of the dimer, respectively. The binding of Tar to the active site caused slight rearrangements in the structure and dynamics of the enzyme, acting as a weak inhibitor of Fos binding. Differential scanning fluorimetry (DSF) was used to measure the thermal stability of FosAKP under different conditions, allowing for the selection of a suitable buffer to maximize enzyme operational stability. FosAKP displays absolute specificity towards Fos; therefore, this enzyme was exploited for the development of an enzyme-based colorimetric biosensor. FosAKP was tethered at the bottom of a plastic cuvette using glutaraldehyde chemistry to develop a simple colorimetric method for the determination of Fos in drinking water and animal plasma.

-	~~Authors: Papageorgiou, A.C~~., Varotsou, C., ~~Labrou~~, N.E.	+	Structural Studies of Klebsiella pneumoniae Fosfomycin-Resistance Protein and Its Application for the Development of an Optical Biosensor for Fosfomycin Determination.,Varotsou C, Ataya F, Papageorgiou AC, Labrou NE Int J Mol Sci. 2023 Dec 20;25(1):85. doi: 10.3390/ijms25010085. PMID:38203259<ref>PMID:38203259</ref>

-	~~Description~~: Klebsiella pneumoniae ~~fosfomycin-resistance protein (FosAKP)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Papageorgiou, A.C~~]]	+	<div class="pdbe-citations 8r37" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Varotsou, C~~]]	+	== References ==
-	[[Category: ~~Labrou, N.E~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Klebsiella pneumoniae]]
		+	[[Category: Large Structures]]
		+	[[Category: Labrou NE]]
		+	[[Category: Papageorgiou AC]]
		+	[[Category: Varotsou C]]

OCA at 09:52, 13 December 2023

2023-12-13T09:52:32Z

←Older revision		Revision as of 09:52, 13 December 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8r37 is ON HOLD	+	The entry 8r37 is ON HOLD until Paper Publication

	Authors: Papageorgiou, A.C., Varotsou, C., Labrou, N.E.		Authors: Papageorgiou, A.C., Varotsou, C., Labrou, N.E.

OCA: Protected "8r37" [edit=sysop:move=sysop]

2023-11-15T05:27:02Z

Protected "8r37" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:27, 15 November 2023

OCA: New page: '''Unreleased structure''' The entry 8r37 is ON HOLD Authors: Papageorgiou, A.C., Varotsou, C., Labrou, N.E. Description: Klebsiella pneumoniae fosfomycin-resistance protein (FosAKP) [...

2023-11-15T05:27:00Z

New page: '''Unreleased structure''' The entry 8r37 is ON HOLD Authors: Papageorgiou, A.C., Varotsou, C., Labrou, N.E. Description: Klebsiella pneumoniae fosfomycin-resistance protein (FosAKP) [...

New page

'''Unreleased structure'''

The entry 8r37 is ON HOLD

Authors: Papageorgiou, A.C., Varotsou, C., Labrou, N.E.

Description: Klebsiella pneumoniae fosfomycin-resistance protein (FosAKP)
[[Category: Unreleased Structures]]
[[Category: Papageorgiou, A.C]]
[[Category: Varotsou, C]]
[[Category: Labrou, N.E]]

8r37 - Revision history

OCA at 11:29, 24 January 2024

OCA at 09:52, 13 December 2023

OCA: Protected "8r37" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8r37 is ON HOLD Authors: Papageorgiou, A.C., Varotsou, C., Labrou, N.E. Description: Klebsiella pneumoniae fosfomycin-resistance protein (FosAKP) [...