OCA at 08:05, 22 May 2024

2024-05-22T08:05:48Z

←Older revision		Revision as of 08:05, 22 May 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8t35 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of K51 acetylated LC3A in complex with the LIR of TP53INP2/DOR==
		+	<StructureSection load='8t35' size='340' side='right'caption='[[8t35]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8t35]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8T35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8T35 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.902Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8t35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8t35 OCA], [https://pdbe.org/8t35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8t35 RCSB], [https://www.ebi.ac.uk/pdbsum/8t35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8t35 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MLP3A_HUMAN MLP3A_HUMAN] Involved in formation of autophagosomal vacuoles (autophagosomes).[https://www.uniprot.org/uniprot/T53I2_HUMAN T53I2_HUMAN] Dual regulator of transcription and autophagy. Positively regulates autophagy and is required for autophagosome formation and processing. May act as a scaffold protein that recruits MAP1LC3A, GABARAP and GABARAPL2 and brings them to the autophagosome membrane by interacting with VMP1 where, in cooperation with the BECN1-PI3-kinase class III complex, they trigger autophagosome development. Acts as a transcriptional activator of THRA.<ref>PMID:18030323</ref> <ref>PMID:19056683</ref> <ref>PMID:22470510</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Atg8-family proteins (MAP1LC3/LC3A, LC3B, LC3C, GABARAP, GABARAPL1 and GABARAPL2) play a pivotal role in macroautophagy/autophagy through their ability to help form autophagosomes. Although autophagosomes form in the cytoplasm, nuclear levels of the Atg8-family proteins are significant. Recently, the nuclear/cytoplasmic shuttling of LC3B was shown to require deacetylation of two Lys residues (K49 and K51 in LC3B), which are conserved in Atg8-family proteins. To exit the nucleus, deacetylated LC3B must bind TP53INP2/TP53INP2 (tumor protein p53 inducible nuclear protein 2) through interaction with the LC3-interacting region (LIR) of TP53INP2 (TP53INP2LIR). To examine their selectivity for TP53INP2 and the role of the conserved Lys residues in Atg8-family proteins, we prepared the six human Atg8-family proteins and acetylated variants of LC3A and GABARAP for biophysical and structural characterization of their interactions with the TP53INP2LIR. Isothermal titration calorimetry (ITC) experiments demonstrate that this LIR binds preferentially to GABARAP subfamily proteins, and that only acetylation of the second Lys residue reduces binding to GABARAP and LC3A. Crystal structures of complexes with GABARAP and LC3A (acetylated and deacetylated) define a beta-sheet in the TP53INP2LIR that determines the GABARAP selectivity and establishes the importance of acetylation at the second Lys. The in vitro results were confirmed in cells using acetyl-mimetic variants of GABARAP and LC3A to examine nuclear/cytoplasmic shuttling and colocalization with TP53INP2. Together, the results demonstrate that TP53INP2 shows selectivity to the GABARAP subfamily and acetylation at the second Lys of GABARAP and LC3A disrupts key interactions with TP53INP2 required for their nuclear/cytoplasmic shuttling.

-	~~Authors~~:	+	Structural and functional characterization of the role of acetylation on the interactions of the human Atg8-family proteins with the autophagy receptor TP53INP2/DOR.,Ali MG, Wahba HM, Igelmann S, Cyr N, Ferbeyre G, Omichinski JG Autophagy. 2024 May 10. doi: 10.1080/15548627.2024.2353443. PMID:38726830<ref>PMID:38726830</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8t35" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Ali MGH]]
		+	[[Category: Cyr N]]
		+	[[Category: Omichinski JG]]
		+	[[Category: Wahba HM]]

OCA at 08:30, 21 June 2023

2023-06-21T08:30:09Z

←Older revision		Revision as of 08:30, 21 June 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8t35 is ON HOLD	+	The entry 8t35 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8t35" [edit=sysop:move=sysop]

2023-06-14T06:53:43Z

Protected "8t35" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:53, 14 June 2023

OCA: New page: '''Unreleased structure''' The entry 8t35 is ON HOLD Authors: Description: Category: Unreleased Structures

2023-06-14T06:53:42Z

New page: '''Unreleased structure''' The entry 8t35 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8t35 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8t35 - Revision history

OCA at 08:05, 22 May 2024

OCA at 08:30, 21 June 2023

OCA: Protected "8t35" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8t35 is ON HOLD Authors: Description: Category: Unreleased Structures