8u0z - Revision history

OCA at 10:10, 17 January 2024

2024-01-17T10:10:25Z

←Older revision		Revision as of 10:10, 17 January 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8u0z is ~~ON HOLD until Paper~~ Publication	+	==CRYSTAL STRUCTURE OF THE OROTIDINE 5'-MONOPHOSPHATE DECARBOXYLASE DOMAIN OF Coffea arabica UMP SYNTHASE==
		+	<StructureSection load='8u0z' size='340' side='right'caption='[[8u0z]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8u0z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coffea_arabica Coffea arabica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U0Z FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.399Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N:ANY+5-MONOPHOSPHATE+NUCLEOTIDE'>N</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u0z OCA], [https://pdbe.org/8u0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u0z RCSB], [https://www.ebi.ac.uk/pdbsum/8u0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u0z ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6P6VUE3_COFAR A0A6P6VUE3_COFAR]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In higher eukaryotes and plants, the last two sequential steps in the de novo biosynthesis of uridine 5'-monophosphate (UMP) are catalyzed by a bifunctional natural chimeric protein called UMP synthase (UMPS). In higher plants, UMPS consists of two naturally fused enzymes: orotate phosphoribosyltransferase (OPRTase) at N-terminal and orotidine-5'-monophosphate decarboxylase (ODCase) at C-terminal. In this work, we obtained the full functional recombinant protein UMPS from Coffea arabica (CaUMPS) and studied its structure-function relationships. A biochemical and structural characterization of a plant UMPS with its two functional domains is described together with the presentation of the first crystal structure of a plant ODCase at 1.4 A resolution. The kinetic parameters measured of CaOPRTase and CaODCase domains were comparable to those reported. The crystallographic structure revealed that CaODCase is a dimer that conserves the typical fold observed in other ODCases from prokaryote and eukaryote with a 1-deoxy-ribofuranose-5'-phosphate molecule bound in the active site of one subunit induced a closed conformation. Our results add to the knowledge of one of the key enzymes of the de novo biosynthesis of pyrimidines in plant metabolism and open the door to future applications.

-	~~Authors:~~ Hinojosa-Cruz, A., Diaz-~~Vilchis~~, A., Gonzalez-Segura, L.	+	Structural and functional properties of uridine 5'-monophosphate synthase from Coffea arabica.,Hinojosa-Cruz A, Diaz-Sanchez AG, Diaz-Vilchis A, Gonzalez-Segura L Int J Biol Macromol. 2024 Jan 4;259(Pt 1):129226. doi: , 10.1016/j.ijbiomac.2024.129226. PMID:38184030<ref>PMID:38184030</ref>

-	~~Description: CRYSTAL STRUCTURE OF THE OROTIDINE 5''~~-~~MONOPHOSPHATE DECARBOXYLASE DOMAIN OF~~ Coffea arabica ~~UMP SYNTHASE~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Diaz-Vilchis, A]]	+	<div class="pdbe-citations 8u0z" style="background-color:#fffaf0;"></div>
-	[[Category: Gonzalez-Segura, L]]	+	== References ==
-	[[Category: Hinojosa-Cruz, A]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Coffea arabica]]
		+	[[Category: Large Structures]]
		+	[[Category: Diaz-Vilchis A]]
		+	[[Category: Gonzalez-Segura L]]
		+	[[Category: Hinojosa-Cruz A]]

OCA at 05:55, 25 October 2023

2023-10-25T05:55:51Z

←Older revision		Revision as of 05:55, 25 October 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8u0z is ON HOLD	+	The entry 8u0z is ON HOLD until Paper Publication

	Authors: Hinojosa-Cruz, A., Diaz-Vilchis, A., Gonzalez-Segura, L.		Authors: Hinojosa-Cruz, A., Diaz-Vilchis, A., Gonzalez-Segura, L.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Diaz-Vilchis, A]]		[[Category: Diaz-Vilchis, A]]
-	[[Category: Hinojosa-Cruz, A]]
	[[Category: Gonzalez-Segura, L]]		[[Category: Gonzalez-Segura, L]]
		+	[[Category: Hinojosa-Cruz, A]]

OCA at 12:19, 18 October 2023

2023-10-18T12:19:57Z

←Older revision		Revision as of 12:19, 18 October 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8u0z is ON HOLD ~~until Paper Publication~~	+	The entry 8u0z is ON HOLD

	Authors: Hinojosa-Cruz, A., Diaz-Vilchis, A., Gonzalez-Segura, L.		Authors: Hinojosa-Cruz, A., Diaz-Vilchis, A., Gonzalez-Segura, L.

OCA at 05:07, 27 September 2023

2023-09-27T05:07:17Z

←Older revision		Revision as of 05:07, 27 September 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8u0z is ON HOLD	+	The entry 8u0z is ON HOLD until Paper Publication

-	Authors:	+	Authors: Hinojosa-Cruz, A., Diaz-Vilchis, A., Gonzalez-Segura, L.

-	Description:	+	Description: CRYSTAL STRUCTURE OF THE OROTIDINE 5''-MONOPHOSPHATE DECARBOXYLASE DOMAIN OF Coffea arabica UMP SYNTHASE
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Diaz-Vilchis, A]]
		+	[[Category: Hinojosa-Cruz, A]]
		+	[[Category: Gonzalez-Segura, L]]

OCA: Protected "8u0z" [edit=sysop:move=sysop]

2023-09-06T04:51:45Z

Protected "8u0z" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:51, 6 September 2023

OCA: New page: '''Unreleased structure''' The entry 8u0z is ON HOLD Authors: Description: Category: Unreleased Structures

2023-09-06T04:51:42Z

New page: '''Unreleased structure''' The entry 8u0z is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8u0z is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]