OCA at 07:34, 22 November 2023

2023-11-22T07:34:40Z

←Older revision		Revision as of 07:34, 22 November 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8u10 is ~~ON HOLD until Paper~~ Publication	+	==In situ cryo-EM structure of bacteriophage P22 gp1:gp4:gp5:gp10:gp9 N-term complex in conformation 1 at 3.2A resolution==
		+	<StructureSection load='8u10' size='340' side='right'caption='[[8u10]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8u10]] is a 58 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U10 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U10 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u10 OCA], [https://pdbe.org/8u10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u10 RCSB], [https://www.ebi.ac.uk/pdbsum/8u10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u10 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/EXLYS_BPP22 EXLYS_BPP22] Tail protein located at the vertex occupied by the portal ring. Together with gp10 and gp26, gp4 is required for stabilization of the condensed DNA within the capsid; perhaps by plugging the hole through which the DNA enters. Plays a role in ejection of the bacteriophage DNA into the host cell at the initiation of infection. Functions as an exolysin that catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycans.<ref>PMID:14763988</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteriophage P22 is a prototypical member of the Podoviridae superfamily. Since its discovery in 1952, P22 has become a paradigm for phage transduction and a model for icosahedral viral capsid assembly. Here, we describe the complete architecture of the P22 tail apparatus (gp1, gp4, gp10, gp9, and gp26) and the potential location and organization of P22 ejection proteins (gp7, gp20, and gp16), determined using cryo-EM localized reconstruction, genetic knockouts, and biochemical analysis. We found that the tail apparatus exists in two equivalent conformations, rotated by approximately 6 degrees relative to the capsid. Portal protomers make unique contacts with coat subunits in both conformations, explaining the 12:5 symmetry mismatch. The tail assembles around the hexameric tail hub (gp10), which folds into an interrupted beta-propeller characterized by an apical insertion domain. The tail hub connects proximally to the dodecameric portal protein and head-to-tail adapter (gp4), distally to the trimeric tail needle (gp26), and laterally to six trimeric tailspikes (gp9) that attach asymmetrically to gp10 insertion domain. Cryo-EM analysis of P22 mutants lacking the ejection proteins gp7 or gp20 and biochemical analysis of purified recombinant proteins suggest that gp7 and gp20 form a molecular complex associated with the tail apparatus via the portal protein barrel. We identified a putative signal transduction pathway from the tailspike to the tail needle, mediated by three flexible loops in the tail hub, that explains how lipopolysaccharide (LPS) is sufficient to trigger the ejection of the P22 DNA in vitro.

-	~~Authors:~~ Iglesias, S., ~~Feng-~~Hou, C., ~~Cingolani~~, G.	+	Molecular Architecture of Salmonella Typhimurium Virus P22 Genome Ejection Machinery.,Iglesias SM, Lokareddy RK, Yang R, Li F, Yeggoni DP, David Hou CF, Leroux MN, Cortines JR, Leavitt JC, Bird M, Casjens SR, White S, Teschke CM, Cingolani G J Mol Biol. 2023 Nov 10;435(24):168365. doi: 10.1016/j.jmb.2023.168365. PMID:37952769<ref>PMID:37952769</ref>

-	~~Description: In situ cryo-EM structure~~ of ~~bacteriophage P22 gp1:gp4:gp5:gp10:gp9 N-term complex in conformation 1 at 3~~.~~2A resolution~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Cingolani, G]]	+	<div class="pdbe-citations 8u10" style="background-color:#fffaf0;"></div>
-	[[Category: Feng-Hou, C]]	+	== References ==
-	[[Category: Iglesias, S]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Salmonella virus P22]]
		+	[[Category: Cingolani G]]
		+	[[Category: Feng-Hou C]]
		+	[[Category: Iglesias S]]

OCA at 10:58, 4 October 2023

2023-10-04T10:58:47Z

←Older revision		Revision as of 10:58, 4 October 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8u10 is ON HOLD	+	The entry 8u10 is ON HOLD until Paper Publication

	Authors: Iglesias, S., Feng-Hou, C., Cingolani, G.		Authors: Iglesias, S., Feng-Hou, C., Cingolani, G.
Line 7:		Line 7:
	Description: In situ cryo-EM structure of bacteriophage P22 gp1:gp4:gp5:gp10:gp9 N-term complex in conformation 1 at 3.2A resolution		Description: In situ cryo-EM structure of bacteriophage P22 gp1:gp4:gp5:gp10:gp9 N-term complex in conformation 1 at 3.2A resolution
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Iglesias, S]]
	[[Category: Cingolani, G]]		[[Category: Cingolani, G]]
	[[Category: Feng-Hou, C]]		[[Category: Feng-Hou, C]]
		+	[[Category: Iglesias, S]]

OCA: Protected "8u10" [edit=sysop:move=sysop]

2023-09-06T04:51:52Z

Protected "8u10" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:51, 6 September 2023

OCA: New page: '''Unreleased structure''' The entry 8u10 is ON HOLD Authors: Iglesias, S., Feng-Hou, C., Cingolani, G. Description: In situ cryo-EM structure of bacteriophage P22 gp1:gp4:gp5:gp10:gp9...

2023-09-06T04:51:49Z

New page: '''Unreleased structure''' The entry 8u10 is ON HOLD Authors: Iglesias, S., Feng-Hou, C., Cingolani, G. Description: In situ cryo-EM structure of bacteriophage P22 gp1:gp4:gp5:gp10:gp9...

New page

'''Unreleased structure'''

The entry 8u10 is ON HOLD

Authors: Iglesias, S., Feng-Hou, C., Cingolani, G.

Description: In situ cryo-EM structure of bacteriophage P22 gp1:gp4:gp5:gp10:gp9 N-term complex in conformation 1 at 3.2A resolution
[[Category: Unreleased Structures]]
[[Category: Iglesias, S]]
[[Category: Cingolani, G]]
[[Category: Feng-Hou, C]]

8u10 - Revision history

OCA at 07:34, 22 November 2023

OCA at 10:58, 4 October 2023

OCA: Protected "8u10" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8u10 is ON HOLD Authors: Iglesias, S., Feng-Hou, C., Cingolani, G. Description: In situ cryo-EM structure of bacteriophage P22 gp1:gp4:gp5:gp10:gp9...