8u1o - Revision history

OCA at 15:05, 13 March 2024

2024-03-13T15:05:07Z

←Older revision		Revision as of 15:05, 13 March 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A5Y1TVZ2_SALER A0A5Y1TVZ2_SALER]		[https://www.uniprot.org/uniprot/A0A5Y1TVZ2_SALER A0A5Y1TVZ2_SALER]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bacteriophage P22 is a prototypical member of the Podoviridae superfamily. Since its discovery in 1952, P22 has become a paradigm for phage transduction and a model for icosahedral viral capsid assembly. Here, we describe the complete architecture of the P22 tail apparatus (gp1, gp4, gp10, gp9, and gp26) and the potential location and organization of P22 ejection proteins (gp7, gp20, and gp16), determined using cryo-EM localized reconstruction, genetic knockouts, and biochemical analysis. We found that the tail apparatus exists in two equivalent conformations, rotated by approximately 6 degrees relative to the capsid. Portal protomers make unique contacts with coat subunits in both conformations, explaining the 12:5 symmetry mismatch. The tail assembles around the hexameric tail hub (gp10), which folds into an interrupted beta-propeller characterized by an apical insertion domain. The tail hub connects proximally to the dodecameric portal protein and head-to-tail adapter (gp4), distally to the trimeric tail needle (gp26), and laterally to six trimeric tailspikes (gp9) that attach asymmetrically to gp10 insertion domain. Cryo-EM analysis of P22 mutants lacking the ejection proteins gp7 or gp20 and biochemical analysis of purified recombinant proteins suggest that gp7 and gp20 form a molecular complex associated with the tail apparatus via the portal protein barrel. We identified a putative signal transduction pathway from the tailspike to the tail needle, mediated by three flexible loops in the tail hub, that explains how lipopolysaccharide (LPS) is sufficient to trigger the ejection of the P22 DNA in vitro.

-	Molecular Architecture of Salmonella Typhimurium Virus P22 Genome Ejection Machinery.,Iglesias SM, Lokareddy RK, Yang R, Li F, Yeggoni DP, David Hou CF, Leroux MN, Cortines JR, Leavitt JC, Bird M, Casjens SR, White S, Teschke CM, Cingolani G J Mol Biol. 2023 Nov 10;435(24):168365. doi: 10.1016/j.jmb.2023.168365. PMID:37952769<ref>PMID:37952769</ref>	+	==See Also==
-		+	*[[Tailspike protein 3D structures\|Tailspike protein 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 8u1o" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:11, 29 November 2023

2023-11-29T14:11:51Z

←Older revision		Revision as of 14:11, 29 November 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8u1o is ~~ON HOLD until Paper~~ Publication	+	==In situ cryo-EM structure of bacteriophage P22 tailspike protein complex at 3.4A resolution==
		+	<StructureSection load='8u1o' size='340' side='right'caption='[[8u1o]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8u1o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U1O FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u1o OCA], [https://pdbe.org/8u1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u1o RCSB], [https://www.ebi.ac.uk/pdbsum/8u1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u1o ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A5Y1TVZ2_SALER A0A5Y1TVZ2_SALER]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteriophage P22 is a prototypical member of the Podoviridae superfamily. Since its discovery in 1952, P22 has become a paradigm for phage transduction and a model for icosahedral viral capsid assembly. Here, we describe the complete architecture of the P22 tail apparatus (gp1, gp4, gp10, gp9, and gp26) and the potential location and organization of P22 ejection proteins (gp7, gp20, and gp16), determined using cryo-EM localized reconstruction, genetic knockouts, and biochemical analysis. We found that the tail apparatus exists in two equivalent conformations, rotated by approximately 6 degrees relative to the capsid. Portal protomers make unique contacts with coat subunits in both conformations, explaining the 12:5 symmetry mismatch. The tail assembles around the hexameric tail hub (gp10), which folds into an interrupted beta-propeller characterized by an apical insertion domain. The tail hub connects proximally to the dodecameric portal protein and head-to-tail adapter (gp4), distally to the trimeric tail needle (gp26), and laterally to six trimeric tailspikes (gp9) that attach asymmetrically to gp10 insertion domain. Cryo-EM analysis of P22 mutants lacking the ejection proteins gp7 or gp20 and biochemical analysis of purified recombinant proteins suggest that gp7 and gp20 form a molecular complex associated with the tail apparatus via the portal protein barrel. We identified a putative signal transduction pathway from the tailspike to the tail needle, mediated by three flexible loops in the tail hub, that explains how lipopolysaccharide (LPS) is sufficient to trigger the ejection of the P22 DNA in vitro.

-	~~Authors:~~ Iglesias, ~~S.M.~~, ~~Feng-~~Hou, C., ~~Cingolani~~, G.	+	Molecular Architecture of Salmonella Typhimurium Virus P22 Genome Ejection Machinery.,Iglesias SM, Lokareddy RK, Yang R, Li F, Yeggoni DP, David Hou CF, Leroux MN, Cortines JR, Leavitt JC, Bird M, Casjens SR, White S, Teschke CM, Cingolani G J Mol Biol. 2023 Nov 10;435(24):168365. doi: 10.1016/j.jmb.2023.168365. PMID:37952769<ref>PMID:37952769</ref>

-	~~Description: In situ cryo-EM structure~~ of ~~bacteriophage P22 tailspike protein complex at 3~~.~~4A resolution~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Iglesias, S.M~~]]	+	<div class="pdbe-citations 8u1o" style="background-color:#fffaf0;"></div>
-	[[Category: Cingolani, G]]	+	== References ==
-	[[Category: Feng-Hou, C]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Salmonella enterica]]
		+	[[Category: Cingolani G]]
		+	[[Category: Feng-Hou C]]
		+	[[Category: Iglesias SM]]

OCA at 04:46, 11 October 2023

2023-10-11T04:46:02Z

←Older revision		Revision as of 04:46, 11 October 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8u1o is ON HOLD	+	The entry 8u1o is ON HOLD until Paper Publication

	Authors: Iglesias, S.M., Feng-Hou, C., Cingolani, G.		Authors: Iglesias, S.M., Feng-Hou, C., Cingolani, G.

OCA: Protected "8u1o" [edit=sysop:move=sysop]

2023-09-20T12:35:05Z

Protected "8u1o" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:35, 20 September 2023

OCA: New page: '''Unreleased structure''' The entry 8u1o is ON HOLD Authors: Iglesias, S.M., Feng-Hou, C., Cingolani, G. Description: In situ cryo-EM structure of bacteriophage P22 tailspike protein ...

2023-09-20T12:35:04Z

New page: '''Unreleased structure''' The entry 8u1o is ON HOLD Authors: Iglesias, S.M., Feng-Hou, C., Cingolani, G. Description: In situ cryo-EM structure of bacteriophage P22 tailspike protein ...

New page

'''Unreleased structure'''

The entry 8u1o is ON HOLD

Authors: Iglesias, S.M., Feng-Hou, C., Cingolani, G.

Description: In situ cryo-EM structure of bacteriophage P22 tailspike protein complex at 3.4A resolution
[[Category: Unreleased Structures]]
[[Category: Iglesias, S.M]]
[[Category: Cingolani, G]]
[[Category: Feng-Hou, C]]