8u22 - Revision history

OCA at 08:24, 7 February 2024

2024-02-07T08:24:10Z

←Older revision		Revision as of 08:24, 7 February 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u22 OCA], [https://pdbe.org/8u22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u22 RCSB], [https://www.ebi.ac.uk/pdbsum/8u22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u22 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u22 OCA], [https://pdbe.org/8u22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u22 RCSB], [https://www.ebi.ac.uk/pdbsum/8u22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u22 ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Fluorescent proteins (FPs) are versatile biomarkers that facilitate effective detection and tracking of macromolecules of interest in real time. Engineered FPs such as superfolder green fluorescent protein (sfGFP) and superfolder Cherry (sfCherry) have exceptional refolding capability capable of delivering fluorescent readout in harsh environments where most proteins lose their native functions. Our recent work on the development of a split FP from a species of strawberry anemone, Corynactis californica, delivered pairs of fragments with up to threefold faster complementation than split GFP. We present the biophysical, biochemical, and structural characteristics of five full-length variants derived from these split C. californica GFP (ccGFP). These ccGFP variants are more tolerant under chemical denaturation with up to 8 kcal/mol lower unfolding free energy than that of the sfGFP. It is likely that some of these ccGFP variants could be suitable as biomarkers under more adverse environments where sfGFP fails to survive. A structural analysis suggests explanations of the variations in stabilities among the ccGFP variants.
-
-	Engineering highly stable variants of Corynactis californica green fluorescent proteins.,Hung LW, Terwilliger TC, Waldo GS, Nguyen HB Protein Sci. 2024 Feb;33(2):e4886. doi: 10.1002/pro.4886. PMID:38151801<ref>PMID:38151801</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8u22" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:46, 1 February 2024

2024-02-01T11:46:51Z

←Older revision		Revision as of 11:46, 1 February 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8u22 is ~~ON HOLD~~	+	==A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP 7==
		+	<StructureSection load='8u22' size='340' side='right'caption='[[8u22]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8u22]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynactis_californica Corynactis californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U22 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C12:2-(1-AMINO-2-HYDROXYPROPYL)-4-(4-HYDROXYBENZYL)-1-(2-OXOETHYL)-1H-IMIDAZOL-5-OLATE'>C12</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u22 OCA], [https://pdbe.org/8u22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u22 RCSB], [https://www.ebi.ac.uk/pdbsum/8u22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u22 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fluorescent proteins (FPs) are versatile biomarkers that facilitate effective detection and tracking of macromolecules of interest in real time. Engineered FPs such as superfolder green fluorescent protein (sfGFP) and superfolder Cherry (sfCherry) have exceptional refolding capability capable of delivering fluorescent readout in harsh environments where most proteins lose their native functions. Our recent work on the development of a split FP from a species of strawberry anemone, Corynactis californica, delivered pairs of fragments with up to threefold faster complementation than split GFP. We present the biophysical, biochemical, and structural characteristics of five full-length variants derived from these split C. californica GFP (ccGFP). These ccGFP variants are more tolerant under chemical denaturation with up to 8 kcal/mol lower unfolding free energy than that of the sfGFP. It is likely that some of these ccGFP variants could be suitable as biomarkers under more adverse environments where sfGFP fails to survive. A structural analysis suggests explanations of the variations in stabilities among the ccGFP variants.

-	~~Authors:~~ Hung~~, L.-W.~~, Terwilliger~~, T.C.~~, Waldo~~, G.~~, Nguyen~~, H~~.B.	+	Engineering highly stable variants of Corynactis californica green fluorescent proteins.,Hung LW, Terwilliger TC, Waldo GS, Nguyen HB Protein Sci. 2024 Feb;33(2):e4886. doi: 10.1002/pro.4886. PMID:38151801<ref>PMID:38151801</ref>

-	~~Description: A Highly Stable Variant~~ of Corynactis ~~Californica Green Fluorescent Protein, ccGFP 7~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Nguyen, H.B~~]]	+	<div class="pdbe-citations 8u22" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Terwilliger, T.C~~]]	+	== References ==
-	[[Category: ~~Hung, L.-W~~]]	+	<references/>
-	[[Category: Waldo, G]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Corynactis californica]]
		+	[[Category: Large Structures]]
		+	[[Category: Hung L-W]]
		+	[[Category: Nguyen HB]]
		+	[[Category: Terwilliger TC]]
		+	[[Category: Waldo G]]

OCA: Protected "8u22" [edit=sysop:move=sysop]

2023-09-20T12:35:58Z

Protected "8u22" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:35, 20 September 2023

OCA: New page: '''Unreleased structure''' The entry 8u22 is ON HOLD Authors: Hung, L.-W., Terwilliger, T.C., Waldo, G., Nguyen, H.B. Description: A Highly Stable Variant of Corynactis Californica Gre...

2023-09-20T12:35:55Z

New page: '''Unreleased structure''' The entry 8u22 is ON HOLD Authors: Hung, L.-W., Terwilliger, T.C., Waldo, G., Nguyen, H.B. Description: A Highly Stable Variant of Corynactis Californica Gre...

New page

'''Unreleased structure'''

The entry 8u22 is ON HOLD

Authors: Hung, L.-W., Terwilliger, T.C., Waldo, G., Nguyen, H.B.

Description: A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP 7
[[Category: Unreleased Structures]]
[[Category: Nguyen, H.B]]
[[Category: Terwilliger, T.C]]
[[Category: Hung, L.-W]]
[[Category: Waldo, G]]