OCA at 10:05, 20 December 2023

2023-12-20T10:05:27Z

←Older revision		Revision as of 10:05, 20 December 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8u5e is ~~ON HOLD~~	+	==Crystal Structure of C-terminal domain of Clostridium perfringens Enterotoxin in Space Group P 21 21 21==
		+	<StructureSection load='8u5e' size='340' side='right'caption='[[8u5e]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8u5e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U5E FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u5e OCA], [https://pdbe.org/8u5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u5e RCSB], [https://www.ebi.ac.uk/pdbsum/8u5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u5e ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF] This enterotoxin is responsible for many cases of a mild type of food poisoning.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clostridium perfringens enterotoxin (CpE) is a beta-pore forming toxin that disrupts gastrointestinal homeostasis in mammals by binding membrane protein receptors called claudins. Although structures of CpE fragments bound to claudins have been determined, the mechanisms that trigger CpE activation and oligomerization that lead to the formation of cytotoxic beta-pores remain undetermined. Proteolysis of CpE in the gut by trypsin has been shown to play a role in this and subsequent cytotoxicity processes. Here, we report solution structures of full-length and trypsinized CpE using small-angle X-ray scattering (SAXS) and crystal structures of trypsinized CpE and its C-terminal claudin-binding domain (cCpE) using X-ray crystallography. Mass spectrometry and SAXS uncover that removal of the CpE N-terminus by trypsin alters the CpE structure to expose areas that are normally unexposed. Crystal structures of trypsinized CpE and cCpE reveal unique dimer interfaces that could serve as oligomerization sites. Moreover, comparisons of these structures to existing ones predict the functional implications of oligomerization in the contexts of cell receptor binding and beta-pore formation. This study sheds light on trypsin's role in altering CpE structure to activate its function via inducing oligomerization on its path toward cytotoxic beta-pore formation. Its findings can incite new approaches to inhibit CpE-based cytotoxicity with oligomer-disrupting therapeutics.

-	~~Authors: Kapoor~~, S., Vecchio~~, A~~.J.	+	Structural Basis of Clostridium perfringens Enterotoxin Activation and Oligomerization by Trypsin.,Ogbu CP, Kapoor S, Vecchio AJ Toxins (Basel). 2023 Oct 31;15(11):637. doi: 10.3390/toxins15110637. PMID:37999500<ref>PMID:37999500</ref>

-	~~Description: Crystal Structure~~ of ~~C-terminal domain~~ of Clostridium perfringens ~~Enterotoxin in Space Group P 21 21 21~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Kapoor, S]]	+	<div class="pdbe-citations 8u5e" style="background-color:#fffaf0;"></div>
-	[[Category: Vecchio~~, A.J~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Clostridium perfringens]]
		+	[[Category: Large Structures]]
		+	[[Category: Kapoor S]]
		+	[[Category: Vecchio AJ]]

OCA: Protected "8u5e" [edit=sysop:move=sysop]

2023-09-20T12:43:42Z

Protected "8u5e" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:43, 20 September 2023

OCA: New page: '''Unreleased structure''' The entry 8u5e is ON HOLD Authors: Kapoor, S., Vecchio, A.J. Description: Crystal Structure of C-terminal domain of Clostridium perfringens Enterotoxin in Sp...

2023-09-20T12:43:38Z

New page: '''Unreleased structure''' The entry 8u5e is ON HOLD Authors: Kapoor, S., Vecchio, A.J. Description: Crystal Structure of C-terminal domain of Clostridium perfringens Enterotoxin in Sp...

New page

'''Unreleased structure'''

The entry 8u5e is ON HOLD

Authors: Kapoor, S., Vecchio, A.J.

Description: Crystal Structure of C-terminal domain of Clostridium perfringens Enterotoxin in Space Group P 21 21 21
[[Category: Unreleased Structures]]
[[Category: Kapoor, S]]
[[Category: Vecchio, A.J]]

8u5e - Revision history

OCA at 10:05, 20 December 2023

OCA: Protected "8u5e" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8u5e is ON HOLD Authors: Kapoor, S., Vecchio, A.J. Description: Crystal Structure of C-terminal domain of Clostridium perfringens Enterotoxin in Sp...