OCA at 10:06, 20 December 2023

2023-12-20T10:06:16Z

←Older revision		Revision as of 10:06, 20 December 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8w7s is ~~ON HOLD~~	+	==Yeast replisome in state IV==
		+	<StructureSection load='8w7s' size='340' side='right'caption='[[8w7s]], [[Resolution\|resolution]] 7.39Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8w7s]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8W7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8W7S FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 7.39Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8w7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8w7s OCA], [https://pdbe.org/8w7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8w7s RCSB], [https://www.ebi.ac.uk/pdbsum/8w7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8w7s ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6A5Q1S9_YEASX A0A6A5Q1S9_YEASX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The replisome that replicates the eukaryotic genome consists of at least three engines: the Cdc45-MCM-GINS (CMG) helicase that separates duplex DNA at the replication fork and two DNA polymerases, one on each strand, that replicate the unwound DNA. Here, we determined a series of cryo-electron microscopy structures of a yeast replisome comprising CMG, leading-strand polymerase Polepsilon and three accessory factors on a forked DNA. In these structures, Polepsilon engages or disengages with the motor domains of the CMG by occupying two alternative positions, which closely correlate with the rotational movement of the single-stranded DNA around the MCM pore. During this process, the polymerase remains stably coupled to the helicase using Psf1 as a hinge. This synergism is modulated by a concerted rearrangement of ATPase sites to drive DNA translocation. The Polepsilon-MCM coupling is not only required for CMG formation to initiate DNA replication but also facilitates the leading-strand DNA synthesis mediated by Polepsilon. Our study elucidates a mechanism intrinsic to the replisome that coordinates the activities of CMG and Polepsilon to negotiate any roadblocks, DNA damage, and epigenetic marks encountered during translocation along replication forks.

-	~~Authors: Dang, S~~., ~~Zhai, Y.~~, Feng, J., Yu, D.	+	Synergism between CMG helicase and leading strand DNA polymerase at replication fork.,Xu Z, Feng J, Yu D, Huo Y, Ma X, Lam WH, Liu Z, Li XD, Ishibashi T, Dang S, Zhai Y Nat Commun. 2023 Sep 20;14(1):5849. doi: 10.1038/s41467-023-41506-0. PMID:37730685<ref>PMID:37730685</ref>

-	~~Description~~: ~~Yeast replisome in state IV~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Feng, J~~]]	+	<div class="pdbe-citations 8w7s" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Zhai, Y~~]]	+	== References ==
-	[[Category: Dang, S]]	+	<references/>
-	[[Category: Yu, D]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Saccharomyces cerevisiae S288C]]
		+	[[Category: Dang S]]
		+	[[Category: Feng J]]
		+	[[Category: Yu D]]
		+	[[Category: Zhai Y]]

OCA: Protected "8w7s" [edit=sysop:move=sysop]

2023-09-06T04:59:53Z

Protected "8w7s" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:59, 6 September 2023

OCA: New page: '''Unreleased structure''' The entry 8w7s is ON HOLD Authors: Dang, S., Zhai, Y., Feng, J., Yu, D. Description: Yeast replisome in state IV Category: Unreleased Structures [[Catego...

2023-09-06T04:59:52Z

New page: '''Unreleased structure''' The entry 8w7s is ON HOLD Authors: Dang, S., Zhai, Y., Feng, J., Yu, D. Description: Yeast replisome in state IV Category: Unreleased Structures [[Catego...

New page

'''Unreleased structure'''

The entry 8w7s is ON HOLD

Authors: Dang, S., Zhai, Y., Feng, J., Yu, D.

Description: Yeast replisome in state IV
[[Category: Unreleased Structures]]
[[Category: Feng, J]]
[[Category: Zhai, Y]]
[[Category: Dang, S]]
[[Category: Yu, D]]

8w7s - Revision history

OCA at 10:06, 20 December 2023

OCA: Protected "8w7s" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8w7s is ON HOLD Authors: Dang, S., Zhai, Y., Feng, J., Yu, D. Description: Yeast replisome in state IV Category: Unreleased Structures [[Catego...