OCA at 07:09, 25 October 2023

2023-10-25T07:09:51Z

←Older revision		Revision as of 07:09, 25 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8wl9 is ~~ON HOLD~~	+	==X-ray structure of Enterobacter cloacae allose-binding protein in complex with D-ribose==
		+	<StructureSection load='8wl9' size='340' side='right'caption='[[8wl9]], [[Resolution\|resolution]] 1.93Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8wl9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WL9 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RIP:RIBOSE(PYRANOSE+FORM)'>RIP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wl9 OCA], [https://pdbe.org/8wl9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wl9 RCSB], [https://www.ebi.ac.uk/pdbsum/8wl9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wl9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A7G3F0C7_ENTCL A0A7G3F0C7_ENTCL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	d-Allose is an aldohexose of the C3-epimer of d-glucose, existing in very small amounts in nature, called a rare sugar. The operon responsible for d-allose metabolism, the allose operon, was found in several bacteria, which consists of seven genes: alsR, alsB, alsA, alsC, alsE, alsK, and rpiB. To understand the biological implication of the allose operon utilizing a rare sugar of d-allose as a carbon source, it is important to clarify whether the allose operon functions specifically for d-allose or also functions for other ligands. It was proposed that the allose operon can function for d-ribose, which is essential as a component of nucleotides and abundant in nature. Allose-binding protein, AlsB, coded in the allose operon, is thought to capture a ligand outside the cell, and is expected to show high affinity for the specific ligand. X-ray structure determinations of Enterobacter cloacae AlsB (EtcAlsB) in ligand-free form, and in complexes with d-allose, d-ribose, and d-allulose, and measurements of the thermal parameters of the complex formation using an isothermal titration calorimeter were performed. The results demonstrated that EtcAlsB has a unique recognition mechanism for high affinity to d-allose by changing its conformation from an open to a closed form depending on d-allose-binding, and that the binding of d-ribose to EtcAlsB could not induce a completely closed form but an intermediate form, explaining the low affinity for d-ribose.

-	~~Authors: Kamitori~~, S.	+	X-ray structures of Enterobacter cloacae allose-binding protein in complexes with monosaccharides demonstrate its unique recognition mechanism for high affinity to allose.,Kamitori S Biochem Biophys Res Commun. 2023 Nov 19;682:187-192. doi: , 10.1016/j.bbrc.2023.10.016. Epub 2023 Oct 5. PMID:37820454<ref>PMID:37820454</ref>

-	~~Description: X-ray structure~~ of ~~Enterobacter cloacae allose~~-~~binding protein in complex with D~~-~~ribose~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Kamitori, S]]	+	<div class="pdbe-citations 8wl9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Enterobacter cloacae]]
		+	[[Category: Large Structures]]
		+	[[Category: Kamitori S]]

OCA: Protected "8wl9" [edit=sysop:move=sysop]

2023-10-04T11:20:36Z

Protected "8wl9" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:20, 4 October 2023

OCA: New page: '''Unreleased structure''' The entry 8wl9 is ON HOLD Authors: Kamitori, S. Description: X-ray structure of Enterobacter cloacae allose-binding protein in complex with D-ribose [[Catego...

2023-10-04T11:20:34Z

New page: '''Unreleased structure''' The entry 8wl9 is ON HOLD Authors: Kamitori, S. Description: X-ray structure of Enterobacter cloacae allose-binding protein in complex with D-ribose [[Catego...

New page

'''Unreleased structure'''

The entry 8wl9 is ON HOLD

Authors: Kamitori, S.

Description: X-ray structure of Enterobacter cloacae allose-binding protein in complex with D-ribose
[[Category: Unreleased Structures]]
[[Category: Kamitori, S]]

8wl9 - Revision history

OCA at 07:09, 25 October 2023

OCA: Protected "8wl9" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8wl9 is ON HOLD Authors: Kamitori, S. Description: X-ray structure of Enterobacter cloacae allose-binding protein in complex with D-ribose [[Catego...