OCA at 08:40, 9 May 2024

2024-05-09T08:40:23Z

←Older revision		Revision as of 08:40, 9 May 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8wtb is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of McsA/McsB complex truncated by chymotrypsin==
		+	<StructureSection load='8wtb' size='340' side='right'caption='[[8wtb]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8wtb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WTB FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wtb OCA], [https://pdbe.org/8wtb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wtb RCSB], [https://www.ebi.ac.uk/pdbsum/8wtb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wtb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MCSB_BACSU MCSB_BACSU] Catalyzes the specific phosphorylation of arginine residues in a large number of proteins. Is part of the bacterial stress response system, where it is involved in regulating the global heat shock repressor CtsR; phosphorylates arginine residues in the winged helix-turn-helix domain of CtsR, thereby preventing its binding to DNA and consequently inducing the expression of repressed genes. The transcriptional repressor HrcA, the chaperone GroEL, the unfoldase ClpC, together with several ribosomal subunits, represent other physiological targets of McsB under stress conditions. Protein arginine phosphorylation has a physiologically important role and is involved in the regulation of many critical cellular processes, such as protein homeostasis, motility, competence, and stringent and stress responses, by regulating gene expression and protein activity. Functions as an adapter whose kinase activity is required for ClpCP-mediated degradation of CtsR during heat stress. Is required for the delocalization of competence proteins from the cell poles, probably via a role in the degradation of anchor proteins.<ref>PMID:19226326</ref> <ref>PMID:21622759</ref> <ref>PMID:22517742</ref> <ref>PMID:24263382</ref> <ref>PMID:30962626</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In gram-positive bacteria, phosphorylated arginine functions as a protein degradation signal in a similar manner as ubiquitin in eukaryotes. The protein-arginine phosphorylation is mediated by the McsAB complex, where McsB possesses kinase activity and McsA modulates McsB activity. Although mcsA and mcsB are regulated within the same operon, the role of McsA in kinase activity has not yet been clarified. In this study, we determined the molecular mechanism by which McsA regulates kinase activity. The crystal structure of the McsAB complex shows that McsA binds to the McsB kinase domain through a second zinc-coordination domain and the subsequent loop region. This binding activates McsB kinase activity by rearranging the catalytic site, preventing McsB self-assembly, and enhancing stoichiometric substrate binding. The first zinc-coordination and coiled-coil domains of McsA further activate McsB by reassembling the McsAB oligomer. These results demonstrate that McsA is the regulatory subunit for the reconstitution of the protein-arginine kinase holoenzyme. This study provides structural insight into how protein-arginine kinase directs the cellular protein degradation system.

-	~~Authors~~:	+	Structural insights into the regulation of protein-arginine kinase McsB by McsA.,Arifuzzaman M, Kwon E, Kim DY Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2320312121. doi: , 10.1073/pnas.2320312121. Epub 2024 Apr 16. PMID:38625935<ref>PMID:38625935</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8wtb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
		+	[[Category: Large Structures]]
		+	[[Category: Arifuzzaman M]]
		+	[[Category: Kim DY]]
		+	[[Category: Kwon E]]

OCA at 09:49, 29 November 2023

2023-11-29T09:49:55Z

←Older revision		Revision as of 09:49, 29 November 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8wtb is ON HOLD	+	The entry 8wtb is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8wtb" [edit=sysop:move=sysop]

2023-10-25T06:10:24Z

Protected "8wtb" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:10, 25 October 2023

OCA: New page: '''Unreleased structure''' The entry 8wtb is ON HOLD Authors: Description: Category: Unreleased Structures

2023-10-25T06:10:23Z

New page: '''Unreleased structure''' The entry 8wtb is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8wtb is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8wtb - Revision history

OCA at 08:40, 9 May 2024

OCA at 09:49, 29 November 2023

OCA: Protected "8wtb" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8wtb is ON HOLD Authors: Description: Category: Unreleased Structures