Corticosteroid-binding globulin - Revision history

http://52.214.119.220/wiki/index.php?action=history&feed=atom&title=Corticosteroid-binding_globulin Corticosteroid-binding globulin - Revision history 2026-04-05T10:20:49Z Revision history for this page on the wiki MediaWiki 1.11.2 http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=3701801&oldid=prev Michal Harel at 09:57, 19 January 2023 2023-01-19T09:57:04Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 09:57, 19 January 2023</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Corticosteroid-binding globulin''' (CBG) is a blood plasma protein 50 to 60 kDa in size <ref>PMID:13395526 </ref>,<ref>PMID:7074030 </ref>. Although it is a member of the serine protease inhibitor (SERPIN) structural family, it does not have any known intrinsic serine protease inhibitor activity <ref>PMID:3143075 </ref>. Its physiological function is conventionally thought to be the transport of the weakly water-soluble hormone, cortisol, throughout the circulation <ref>PMID:13395526 </ref>,<ref>PMID:7074030 </ref>. Structural studies of native rat CBG <ref>PMID:17644521 </ref> and a cleaved human CBG-antitrypsin chimera <ref>PMID:18513745 </ref> have corroborated earlier biochemical studies showing that cortisol binds to CBG with a one-to-one stoichiometry. Additionally, CBG is believed to buffer the concentration of free cortisol in the blood, keeping the level constant despite its pulsatile secretion pattern <ref>PMID:9751481 </ref>. CBG is also thought to participate in the stress response by releasing cortisol specifically at inflammatory sites upon cleavage by human neutrophil elastase between residues 344 and 345<ref>PMID:3143075 </ref>.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Corticosteroid-binding globulin''' (CBG) is a blood plasma protein 50 to 60 kDa in size <ref>PMID:13395526 </ref>,<ref>PMID:7074030 </ref>. Although it is a member of the serine protease inhibitor (SERPIN) structural family, it does not have any known intrinsic serine protease inhibitor activity <ref>PMID:3143075 </ref>. Its physiological function is conventionally thought to be the transport of the weakly water-soluble hormone, cortisol, throughout the circulation <ref>PMID:13395526 </ref>,<ref>PMID:7074030 </ref>. Structural studies of native rat CBG <ref>PMID:17644521 </ref> and a cleaved human CBG-antitrypsin chimera <ref>PMID:18513745 </ref> have corroborated earlier biochemical studies showing that cortisol binds to CBG with a one-to-one stoichiometry. Additionally, CBG is believed to buffer the concentration of free cortisol in the blood, keeping the level constant despite its pulsatile secretion pattern <ref>PMID:9751481 </ref>. CBG is also thought to participate in the stress response by releasing cortisol specifically at inflammatory sites upon cleavage by human neutrophil elastase between residues 344 and 345<ref>PMID:3143075 </ref>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>See also: [[Corticosteroids]].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>See also: [[Corticosteroids<ins style="color: red; font-weight: bold; text-decoration: none;">]] and [[Steroid Hormones and their receptors</ins>]].</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Gene==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Gene==</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=3693272&oldid=prev Michal Harel at 09:08, 9 January 2023 2023-01-09T09:08:47Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 09:08, 9 January 2023</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 4:</td> <td colspan="2" class="diff-lineno">Line 4:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Corticosteroid-binding globulin''' (CBG) is a blood plasma protein 50 to 60 kDa in size <ref>PMID:13395526 </ref>,<ref>PMID:7074030 </ref>. Although it is a member of the serine protease inhibitor (SERPIN) structural family, it does not have any known intrinsic serine protease inhibitor activity <ref>PMID:3143075 </ref>. Its physiological function is conventionally thought to be the transport of the weakly water-soluble hormone, cortisol, throughout the circulation <ref>PMID:13395526 </ref>,<ref>PMID:7074030 </ref>. Structural studies of native rat CBG <ref>PMID:17644521 </ref> and a cleaved human CBG-antitrypsin chimera <ref>PMID:18513745 </ref> have corroborated earlier biochemical studies showing that cortisol binds to CBG with a one-to-one stoichiometry. Additionally, CBG is believed to buffer the concentration of free cortisol in the blood, keeping the level constant despite its pulsatile secretion pattern <ref>PMID:9751481 </ref>. CBG is also thought to participate in the stress response by releasing cortisol specifically at inflammatory sites upon cleavage by human neutrophil elastase between residues 344 and 345<ref>PMID:3143075 </ref>.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Corticosteroid-binding globulin''' (CBG) is a blood plasma protein 50 to 60 kDa in size <ref>PMID:13395526 </ref>,<ref>PMID:7074030 </ref>. Although it is a member of the serine protease inhibitor (SERPIN) structural family, it does not have any known intrinsic serine protease inhibitor activity <ref>PMID:3143075 </ref>. Its physiological function is conventionally thought to be the transport of the weakly water-soluble hormone, cortisol, throughout the circulation <ref>PMID:13395526 </ref>,<ref>PMID:7074030 </ref>. Structural studies of native rat CBG <ref>PMID:17644521 </ref> and a cleaved human CBG-antitrypsin chimera <ref>PMID:18513745 </ref> have corroborated earlier biochemical studies showing that cortisol binds to CBG with a one-to-one stoichiometry. Additionally, CBG is believed to buffer the concentration of free cortisol in the blood, keeping the level constant despite its pulsatile secretion pattern <ref>PMID:9751481 </ref>. CBG is also thought to participate in the stress response by releasing cortisol specifically at inflammatory sites upon cleavage by human neutrophil elastase between residues 344 and 345<ref>PMID:3143075 </ref>.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>See also: [[Corticosteroids]].</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Gene==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Gene==</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=3412082&oldid=prev Michal Harel at 09:54, 10 June 2021 2021-06-10T09:54:09Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 09:54, 10 June 2021</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 52:</td> <td colspan="2" class="diff-lineno">Line 52:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Corticosteroid-binding globulin is a very important blood protein that functions not merely as a passive carrier of cortisol, but also as a ready reservoir of the hormone for release at sites of inflammation. It also acts as a buffer to keep the level of blood cortisol constant and thus, maintains homeostasis. Work is still being done, not just in fully elucidating the molecular mechanism of its function, but also in its interaction with various tissues in the body, and this can have great implications on our understanding of metabolic diseases, as well as infection and immunity.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Corticosteroid-binding globulin is a very important blood protein that functions not merely as a passive carrier of cortisol, but also as a ready reservoir of the hormone for release at sites of inflammation. It also acts as a buffer to keep the level of blood cortisol constant and thus, maintains homeostasis. Work is still being done, not just in fully elucidating the molecular mechanism of its function, but also in its interaction with various tissues in the body, and this can have great implications on our understanding of metabolic diseases, as well as infection and immunity.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></StructureSection></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></StructureSection></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==3D structures of corticosteroid-binding <del style="color: red; font-weight: bold; text-decoration: none;">protein</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==3D structures of corticosteroid-binding <ins style="color: red; font-weight: bold; text-decoration: none;">globulin</ins>==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=2792689&oldid=prev Michal Harel at 15:00, 19 October 2017 2017-10-19T15:00:23Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:00, 19 October 2017</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 74:</td> <td colspan="2" class="diff-lineno">Line 74:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><references/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><references/></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category:Topic Page]]</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=2734889&oldid=prev Michal Harel at 10:17, 4 April 2017 2017-04-04T10:17:21Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:17, 4 April 2017</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 57:</td> <td colspan="2" class="diff-lineno">Line 57:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2v95]] – rSer-A6 – rat<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2v95]] – rSer-A6 – rat<br /></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[2vdx]] – hSer-A6 (mutant) - human<br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[2vdx<ins style="color: red; font-weight: bold; text-decoration: none;">]], [[4c41</ins>]] – hSer-A6 (mutant) - human<br /></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>‪[[2vdy]] – hSer-A6 (mutant) + cortisol‬<br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>‪[[2vdy<ins style="color: red; font-weight: bold; text-decoration: none;">]], [[4c49</ins>]] – hSer-A6 (mutant) + cortisol‬<br /></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[4bb2]] – hSer-A6 (mutant) + progesterone <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[4bb2]] – hSer-A6 (mutant) + progesterone <br /></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=2409375&oldid=prev Alexander Berchansky at 13:01, 9 June 2015 2015-06-09T13:01:14Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:01, 9 June 2015</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 32:</td> <td colspan="2" class="diff-lineno">Line 32:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Cbg.jpg|400px|left|thumb| Proposed mechanism of cortisol release at sites of inflammation]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Cbg.jpg|400px|left|thumb| Proposed mechanism of cortisol release at sites of inflammation]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{Clear}}</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Naturally-occurring genetic variants<ref>PMID: 19643161 </ref>==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Naturally-occurring genetic variants<ref>PMID: 19643161 </ref>==</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 51:</td> <td colspan="2" class="diff-lineno">Line 51:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Corticosteroid-binding globulin is a very important blood protein that functions not merely as a passive carrier of cortisol, but also as a ready reservoir of the hormone for release at sites of inflammation. It also acts as a buffer to keep the level of blood cortisol constant and thus, maintains homeostasis. Work is still being done, not just in fully elucidating the molecular mechanism of its function, but also in its interaction with various tissues in the body, and this can have great implications on our understanding of metabolic diseases, as well as infection and immunity.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Corticosteroid-binding globulin is a very important blood protein that functions not merely as a passive carrier of cortisol, but also as a ready reservoir of the hormone for release at sites of inflammation. It also acts as a buffer to keep the level of blood cortisol constant and thus, maintains homeostasis. Work is still being done, not just in fully elucidating the molecular mechanism of its function, but also in its interaction with various tissues in the body, and this can have great implications on our understanding of metabolic diseases, as well as infection and immunity.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></StructureSection></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==3D structures of corticosteroid-binding protein==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==3D structures of corticosteroid-binding protein==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> </table>

Alexander Berchansky http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=2409374&oldid=prev Alexander Berchansky at 13:00, 9 June 2015 2015-06-09T13:00:10Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:00, 9 June 2015</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del style="color: red; font-weight: bold; text-decoration: none;">!-- </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins style="color: red; font-weight: bold; text-decoration: none;">StructureSection load='2V95' size='450' side='right' scene='' caption='Cartoon representation of corticosteroid</ins>-<ins style="color: red; font-weight: bold; text-decoration: none;">binding globulin complex with cortisol, [[2v95]]'</ins>></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Or use the four-green-boxes-button to insert scrollable text adjacent</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">to a Jmol applet. Check out the other buttons as well! </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">-</del>-></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:2v95.png|left|200px|thumb|Crystal Structure of corticosteroid-binding globulin complex with cortisol, [[2v95]]]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:2v95.png|left|200px|thumb|Crystal Structure of corticosteroid-binding globulin complex with cortisol, [[2v95]]]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Introduction==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Introduction==</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 19:</td> <td colspan="2" class="diff-lineno">Line 15:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously-solved structures of the human CBG-antitrypsin (Pittsburgh) chimera with cleaved reactive centre loop at 1.84Å and the native rat CBG at 1.9Å confirm that corticosteroid-binding globulin, despite being a non-inhibitory member of the serpin family, undergoes the S-to-R transition just like the inhibitory members <ref>PMID:17644521 </ref>,<ref>PMID:18513745 </ref>.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously-solved structures of the human CBG-antitrypsin (Pittsburgh) chimera with cleaved reactive centre loop at 1.84Å and the native rat CBG at 1.9Å confirm that corticosteroid-binding globulin, despite being a non-inhibitory member of the serpin family, undergoes the S-to-R transition just like the inhibitory members <ref>PMID:17644521 </ref>,<ref>PMID:18513745 </ref>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In its native form, CBG is in the "stressed" conformation and binds cortisol with very high affinity<ref>PMID:17644521 </ref>.<del style="color: red; font-weight: bold; text-decoration: none;"><Structure load='2V95' size='400' frame='true' align='right' caption='Cartoon representation of corticosteroid-binding globulin complex with cortisol, [[2v95]]' scene='Insert optional scene name here' /> </del>Upon cleavage by its target proteinase, believed to be human neutrophil elastase, the protein undergoes structural rearrangements in the main chain at several parts of the serpin fold, resulting in what is conventionally known as the "relaxed" state <ref>PMID:18513745 </ref>,<ref>PMID:3143075 </ref>, as seen <scene name='Corticosteroid-binding_globulin/Cleaved/9'>here</scene>. The main conformational change is the insertion of the entire N-terminal segment of the reactive loop into beta-sheet A where it is incorporated as a <scene name='Corticosteroid-binding_globulin/Cleaved/10'>novel beta-strand</scene>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In its native form, CBG is in the "stressed" conformation and binds cortisol with very high affinity<ref>PMID:17644521 </ref>.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Upon cleavage by its target proteinase, believed to be human neutrophil elastase, the protein undergoes structural rearrangements in the main chain at several parts of the serpin fold, resulting in what is conventionally known as the "relaxed" state <ref>PMID:18513745 </ref>,<ref>PMID:3143075 </ref>, as seen <scene name='Corticosteroid-binding_globulin/Cleaved/9'>here</scene>. The main conformational change is the insertion of the entire N-terminal segment of the reactive loop into beta-sheet A where it is incorporated as a <scene name='Corticosteroid-binding_globulin/Cleaved/10'>novel beta-strand</scene>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''(Click'' <scene name='Corticosteroid-binding_globulin/Native/1'>here</scene> ''to return to structure of CBG in the S-state.)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''(Click'' <scene name='Corticosteroid-binding_globulin/Native/1'>here</scene> ''to return to structure of CBG in the S-state.)</div></td></tr> </table>

Alexander Berchansky http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=1748932&oldid=prev Michal Harel at 12:56, 24 March 2013 2013-03-24T12:56:29Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:56, 24 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 4:</td> <td colspan="2" class="diff-lineno">Line 4:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>to a Jmol applet. Check out the other buttons as well! </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>to a Jmol applet. Check out the other buttons as well! </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:2v95.png|left|200px|thumb|Crystal Structure of <del style="color: red; font-weight: bold; text-decoration: none;">Bovine Aconitase</del>, [[2v95]]]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:2v95.png|left|200px|thumb|Crystal Structure of <ins style="color: red; font-weight: bold; text-decoration: none;">corticosteroid-binding globulin complex with cortisol</ins>, [[2v95]]]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Introduction==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Introduction==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=1748931&oldid=prev Michal Harel at 12:55, 24 March 2013 2013-03-24T12:55:29Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:55, 24 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 4:</td> <td colspan="2" class="diff-lineno">Line 4:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>to a Jmol applet. Check out the other buttons as well! </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>to a Jmol applet. Check out the other buttons as well! </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:2v95.png|left|200px|thumb|Crystal Structure of Bovine Aconitase, [[2v95]]]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Introduction==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Introduction==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2" class="diff-lineno">Line 62:</td> <td colspan="2" class="diff-lineno">Line 62:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2vdx]] – hSer-A6 (mutant) - human<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2vdx]] – hSer-A6 (mutant) - human<br /></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>‪[[2vdy]] – hSer-A6 (mutant) + cortisol‬<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>‪[[2vdy]] – hSer-A6 (mutant) + cortisol‬<br /></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[4bb2]] – hSer-A6 (mutant) + progesterone /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[4bb2]] – hSer-A6 (mutant) + progesterone <ins style="color: red; font-weight: bold; text-decoration: none;"><br </ins>/></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Resources==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Resources==</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Corticosteroid-binding_globulin&diff=1748930&oldid=prev Michal Harel at 12:53, 24 March 2013 2013-03-24T12:53:11Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:53, 24 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 56:</td> <td colspan="2" class="diff-lineno">Line 56:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==3D structures of corticosteroid-binding protein==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==3D structures of corticosteroid-binding protein==</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2v95]] – rSer-A6 – rat<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2v95]] – rSer-A6 – rat<br /></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2vdx]] – hSer-A6 (mutant) - human<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2vdx]] – hSer-A6 (mutant) - human<br /></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>‪[[<del style="color: red; font-weight: bold; text-decoration: none;">2vdx</del>]] – hSer-A6 (mutant) + cortisol‬</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>‪[[<ins style="color: red; font-weight: bold; text-decoration: none;">2vdy</ins>]] – hSer-A6 (mutant) + cortisol‬<ins style="color: red; font-weight: bold; text-decoration: none;"><br /></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[4bb2]] – hSer-A6 (mutant) + progesterone /></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Resources==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Resources==</div></td></tr> </table>

Michal Harel