Enoylpyruvate transferase - Revision history

Alexander Berchansky at 13:18, 19 July 2021

2021-07-19T13:18:29Z

←Older revision		Revision as of 13:18, 19 July 2021
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	== Structural highlights ==		== Structural highlights ==

-	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/15'>fosfomycin</scene> and <scene name='55/551189/Cv/19'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.	+	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/15'>fosfomycin</scene> and <scene name='55/551189/Cv/20'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.

	== 3D Structures of enoylpyruvate transferase ==		== 3D Structures of enoylpyruvate transferase ==

Alexander Berchansky at 13:14, 19 July 2021

2021-07-19T13:14:50Z

←Older revision		Revision as of 13:14, 19 July 2021
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	== Structural highlights ==		== Structural highlights ==

-	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/15'>fosfomycin</scene> and <scene name='55/551189/Cv/12'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.	+	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/15'>fosfomycin</scene> and <scene name='55/551189/Cv/19'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.

	== 3D Structures of enoylpyruvate transferase ==		== 3D Structures of enoylpyruvate transferase ==

Alexander Berchansky at 11:33, 19 July 2021

2021-07-19T11:33:35Z

←Older revision		Revision as of 11:33, 19 July 2021
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	== Structural highlights ==		== Structural highlights ==

-	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/14'>fosfomycin</scene> and <scene name='55/551189/Cv/12'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.	+	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/15'>fosfomycin</scene> and <scene name='55/551189/Cv/12'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.

	== 3D Structures of enoylpyruvate transferase ==		== 3D Structures of enoylpyruvate transferase ==

Alexander Berchansky at 11:29, 19 July 2021

2021-07-19T11:29:41Z

←Older revision		Revision as of 11:29, 19 July 2021
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	== Function ==		== Function ==

-	'''Enoylpyruvate transferase''' (MurA) or '''UDP-N-acetylglucosamine 1-carboxyvinyltransferase''' catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls.<ref>PMID:7608103</ref>. '''MurC''' or '''UDP-N-acetylmuramate-L-alanine ligase''' adds L-alanine in the biosynthesis of peptidoglycans which form part of the protective bacterial cell wall<ref>PMID:25114134</ref>.	+	'''Enoylpyruvate transferase''' (MurA) or '''UDP-N-acetylglucosamine 1-carboxyvinyltransferase''' catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls.<ref>PMID:7608103</ref>. '''MurC''' or '''UDP-N-acetylmuramate-L-alanine ligase''' adds L-alanine in the biosynthesis of peptidoglycans which form part of the protective bacterial cell wall<ref>PMID:25114134</ref>.

	== Relevance ==		== Relevance ==
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	== Structural highlights ==		== Structural highlights ==

-	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/11'>fosfomycin</scene> and <scene name='55/551189/Cv/12'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.	+	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/14'>fosfomycin</scene> and <scene name='55/551189/Cv/12'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.

	== 3D Structures of enoylpyruvate transferase ==		== 3D Structures of enoylpyruvate transferase ==

Michal Harel at 09:42, 15 July 2021

2021-07-15T09:42:22Z

←Older revision		Revision as of 09:42, 15 July 2021
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	== Function ==		== Function ==

-	'''Enoylpyruvate transferase''' (MurA) or '''UDP-N-acetylglucosamine 1-carboxyvinyltransferase''' catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls.<ref>PMID:7608103</ref>. MurC or UDP-N-acetylmuramate-L-alanine ligase adds L-alanine in the biosynthesis of peptidoglycans which form part of the protective bacterial cell wall<ref>PMID:25114134</ref>.	+	'''Enoylpyruvate transferase''' (MurA) or '''UDP-N-acetylglucosamine 1-carboxyvinyltransferase''' catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls.<ref>PMID:7608103</ref>. '''MurC''' or '''UDP-N-acetylmuramate-L-alanine ligase''' adds L-alanine in the biosynthesis of peptidoglycans which form part of the protective bacterial cell wall<ref>PMID:25114134</ref>.

	== Relevance ==		== Relevance ==

Michal Harel at 09:41, 15 July 2021

2021-07-15T09:41:56Z

←Older revision		Revision as of 09:41, 15 July 2021
Line 2:		Line 2:
	== Function ==		== Function ==

-	'''Enoylpyruvate transferase''' (MurA) or '''UDP-N-acetylglucosamine 1-carboxyvinyltransferase''' catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls.<ref>PMID:7608103</ref>.	+	'''Enoylpyruvate transferase''' (MurA) or '''UDP-N-acetylglucosamine 1-carboxyvinyltransferase''' catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls.<ref>PMID:7608103</ref>. MurC or UDP-N-acetylmuramate-L-alanine ligase adds L-alanine in the biosynthesis of peptidoglycans which form part of the protective bacterial cell wall<ref>PMID:25114134</ref>.

	== Relevance ==		== Relevance ==

Michal Harel at 08:53, 22 July 2020

2020-07-22T08:53:05Z

←Older revision		Revision as of 08:53, 22 July 2020
Line 2:		Line 2:
	== Function ==		== Function ==

-	'''Enoylpyruvate transferase''' (MurA) catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls.<ref>PMID:7608103</ref>.	+	'''Enoylpyruvate transferase''' (MurA) or '''UDP-N-acetylglucosamine 1-carboxyvinyltransferase''' catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls.<ref>PMID:7608103</ref>.

	== Relevance ==		== Relevance ==

Michal Harel at 06:25, 23 June 2019

2019-06-23T06:25:48Z

←Older revision		Revision as of 06:25, 23 June 2019
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	</StructureSection>		</StructureSection>

-	== 3D Structures of enoylpyruvate transferase ==
-
-	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-	{{#tree:id=OrganizedByTopic\|openlevels=0\|
-
-	*MurA
-
-	**[[1naw]], [[1ejc]], [[1ejd]] – EncMurA – ''Enterobacter cloacae''<BR />
-	**[[1dlg]], [[3spb]], [[3v5v]], [[4e7g]], [[4eii]] – EncMurA (mutant) <BR />
-	**[[1uae]] – EcMurA – ''Escherichia coli''<BR />
-	**[[2yvw]] – AaMurA – ''Aquifex aeolicus''<BR />
-	**[[3zh3]], [[3zh4]] – SpMurA – ''Streptococcus pneumoniae''<BR />
-	**[[3r38]] – MurA – ''Listeria monocytogenes''<br />
-	**[[3sg1]] – MurA – ''Bacillus anthracis''<br />
-	**[[5ujs]] – MurA – ''Campylobacter jejuni''<BR />
-
-	*MurA binary complex
-
-	**[[1a2n]] – EcMurA (mutant) + reaction intermediate<BR />
-	**[[3iss]] – EcMurA (mutant) + enoylpyruvyl-UNAG <BR />
-	**[[1eyn]] – EncMurA (mutant) + aniline-naphthalene sulfonate<BR />
-	**[[4e7b]] – EncMurA + UDP-glucose<BR />
-	**[[4e7e]] – EncMurA (mutant) + UDP-glucose<BR />
-	**[[4e7c]] – EncMurA + UTP<BR />
-	**[[4e7d]] – EncMurA + UDP<BR />
-	**[[4e7f]] – EncMurA (mutant) + UDP<BR />
-	**[[1q3g]] – EncMurA (mutant) + reaction intermediate<BR />
-	**[[2rl1]] – HiMurA + UNAG – Haemophilus influenzae<BR />
-	**[[3kqj]] – EcMurA + UNAG <BR />
-	**[[5vm7]] – EcMurA + maturation protein A2 <BR />
-	**[[3upk]] – EncMurA + UNAG <BR />
-	**[[1ybg]] – EcMurA (mutant) + inhibitor<BR />
-	**[[3kqa]] – EncMurA + terreic acid inhibitor<BR />
-	**[[3swq]] – EncMurA (mutant) + enoylpyruvyl-UNAG <BR />
-	**[[3v4t]], [[1ryw]] – EncMurA (mutant) + UNAG <BR />
-	**[[5u4h]] – MurA + phosphonooxy propanoate – ''Acinetobacter baumannii''<BR />
-	**[[6nkj]] – SpMurA + phosphonooxy propanoate <BR />
-	**[[5bq2]] – MurA + enoylpyruvyl-UNAG – ''Pseudomonas aeruginosa''<BR />
-
-	*MurA ternary complex with antibiotics
-
-	**[[3kr6]] – EcMurA + UNAG + fosfomycin<BR />
-	**[[3lth]] – EncMurA + UNAG + fosfomycin<BR />
-	**[[3vcy]] – MurA + UNAG + fosfomycin – ''Vibrio fischeri''<BR />
-	**[[4r7u]] – MurA + UNAG + fosfomycin – ''Vibrio cholerae''<BR />
-	**[[2rl2]] – HiMurA + UNAG + fosfomycin<BR />
-	**[[2z2c]] – EcMurA + UNAG + cnicin<BR />
-
-	*MurA ternary complex
-
-	**[[3su9]], [[3swa]], [[3swi]] – EncMurA (mutant) + UNAG + PEP<BR />
-	**[[3swd]] – EcMurA (mutant) + UNAG + PEP<BR />
-	**[[3swe]] – HiMurA (mutant) + UNAG + PEP<BR />
-	**[[3swg]] – AaMurA + UNAG + PEP<BR />
-	**[[6cn1]] – MurA + phosphonooxy propanoate + UNAG derivative – ''Pseudomonas putida''<BR />
-	**[[5wi5]] – SpMurA + phosphonooxy propanoate + UNAG derivative<BR />
-
-	}}
	== References ==		== References ==
	<references/>		<references/>

	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 06:24, 23 June 2019

2019-06-23T06:24:37Z

←Older revision		Revision as of 06:24, 23 June 2019
Line 11:		Line 11:

	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/11'>fosfomycin</scene> and <scene name='55/551189/Cv/12'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.		MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/11'>fosfomycin</scene> and <scene name='55/551189/Cv/12'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.
		+
		+	== 3D Structures of enoylpyruvate transferase ==
		+	[[Enoylpyruvate transferase 3D structures]]
		+
	</StructureSection>		</StructureSection>

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	**[[1uae]] – EcMurA – ''Escherichia coli''<BR />		**[[1uae]] – EcMurA – ''Escherichia coli''<BR />
	**[[2yvw]] – AaMurA – ''Aquifex aeolicus''<BR />		**[[2yvw]] – AaMurA – ''Aquifex aeolicus''<BR />
-	**[[3zh3]], [[3zh4]] – ~~MurA~~ – ''Streptococcus pneumoniae''<BR />	+	**[[3zh3]], [[3zh4]] – SpMurA – ''Streptococcus pneumoniae''<BR />
	**[[3r38]] – MurA – ''Listeria monocytogenes''<br />		**[[3r38]] – MurA – ''Listeria monocytogenes''<br />
	**[[3sg1]] – MurA – ''Bacillus anthracis''<br />		**[[3sg1]] – MurA – ''Bacillus anthracis''<br />
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	**[[2rl1]] – HiMurA + UNAG – Haemophilus influenzae<BR />		**[[2rl1]] – HiMurA + UNAG – Haemophilus influenzae<BR />
	**[[3kqj]] – EcMurA + UNAG <BR />		**[[3kqj]] – EcMurA + UNAG <BR />
		+	**[[5vm7]] – EcMurA + maturation protein A2 <BR />
	**[[3upk]] – EncMurA + UNAG <BR />		**[[3upk]] – EncMurA + UNAG <BR />
	**[[1ybg]] – EcMurA (mutant) + inhibitor<BR />		**[[1ybg]] – EcMurA (mutant) + inhibitor<BR />
Line 48:		Line 53:
	**[[3v4t]], [[1ryw]] – EncMurA (mutant) + UNAG <BR />		**[[3v4t]], [[1ryw]] – EncMurA (mutant) + UNAG <BR />
	**[[5u4h]] – MurA + phosphonooxy propanoate – ''Acinetobacter baumannii''<BR />		**[[5u4h]] – MurA + phosphonooxy propanoate – ''Acinetobacter baumannii''<BR />
		+	**[[6nkj]] – SpMurA + phosphonooxy propanoate <BR />
	**[[5bq2]] – MurA + enoylpyruvyl-UNAG – ''Pseudomonas aeruginosa''<BR />		**[[5bq2]] – MurA + enoylpyruvyl-UNAG – ''Pseudomonas aeruginosa''<BR />

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	**[[2z2c]] – EcMurA + UNAG + cnicin<BR />		**[[2z2c]] – EcMurA + UNAG + cnicin<BR />

-	*MurA ternary complex ~~with PEP~~	+	*MurA ternary complex

	**[[3su9]], [[3swa]], [[3swi]] – EncMurA (mutant) + UNAG + PEP<BR />		**[[3su9]], [[3swa]], [[3swi]] – EncMurA (mutant) + UNAG + PEP<BR />
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	**[[3swe]] – HiMurA (mutant) + UNAG + PEP<BR />		**[[3swe]] – HiMurA (mutant) + UNAG + PEP<BR />
	**[[3swg]] – AaMurA + UNAG + PEP<BR />		**[[3swg]] – AaMurA + UNAG + PEP<BR />
		+	**[[6cn1]] – MurA + phosphonooxy propanoate + UNAG derivative – ''Pseudomonas putida''<BR />
		+	**[[5wi5]] – SpMurA + phosphonooxy propanoate + UNAG derivative<BR />
		+
	}}		}}
	== References ==		== References ==

Alexander Berchansky at 09:37, 11 March 2019

2019-03-11T09:37:15Z

←Older revision		Revision as of 09:37, 11 March 2019
Line 10:		Line 10:
	== Structural highlights ==		== Structural highlights ==

-	MurA is composed of <scene name='55/551189/Cv/3'>catalytic domain</scene> and <scene name='55/551189/Cv/5'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/8'>fosfomycin</scene> and <scene name='55/551189/Cv/7'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref>	+	MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/11'>fosfomycin</scene> and <scene name='55/551189/Cv/12'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.
	</StructureSection>		</StructureSection>