Galactosyltransferase - Revision history

Alexander Berchansky: Redirecting to Glycosyltransferase

2013-06-20T11:38:24Z

Michal Harel at 12:39, 7 March 2013

2013-03-07T12:39:11Z

←Older revision		Revision as of 12:39, 7 March 2013
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	==3D structures of galactosyltransferase==		==3D structures of galactosyltransferase==

-	''Updated ~~February 2013''~~	+	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}

	===β-1,4-galactosyltransferase (BGT)===		===β-1,4-galactosyltransferase (BGT)===

Michal Harel at 13:11, 3 February 2013

2013-02-03T13:11:44Z

←Older revision		Revision as of 13:11, 3 February 2013
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	==3D structures of galactosyltransferase==		==3D structures of galactosyltransferase==

-	''Updated ~~June 2012~~''	+	''Updated February 2013''

	===β-1,4-galactosyltransferase (BGT)===		===β-1,4-galactosyltransferase (BGT)===
-
-

	[[1fgx]] - bBGT catalytic domain – bovine		[[1fgx]] - bBGT catalytic domain – bovine
Line 23:		Line 21:


-
	===BGT binary complexes===		===BGT binary complexes===


-
	[[1fr8]], [[1o0r]] - bBGT catalytic domain + UDP-galactose		[[1fr8]], [[1o0r]] - bBGT catalytic domain + UDP-galactose

Line 40:		Line 36:
	[[1oqm]] - bBGT catalytic domain + UDP-N-acetylgalactosamine		[[1oqm]] - bBGT catalytic domain + UDP-N-acetylgalactosamine

-	[[2fya]] - hBGT catalytic domain (mutant) + Mn	+	[[2fya]] - hBGT catalytic domain (mutant) + Mn<br />
-		+	[[4ee3]], [[4ee4]], [[4ee5]], [[4eea]], [[4eeg]], [[4eem]], [[4eeo]] - hBGT catalytic domain (mutant) + polysaccharide

-
	===BGT ternary complexes===		===BGT ternary complexes===
-
-

	[[1nhe]], [[1nkh]] - bBGT catalytic domain + UDP + α-lactalbumin		[[1nhe]], [[1nkh]] - bBGT catalytic domain + UDP + α-lactalbumin
Line 69:		Line 62:

	[[2ah9]] - hBGT catalytic domain + UDP + chitotriose		[[2ah9]] - hBGT catalytic domain + UDP + chitotriose
-
-

	===α-1,3-galactosyltransferase (AGT)===		===α-1,3-galactosyltransferase (AGT)===
-
-

	[[1g8o]], [[1fg5]], [[2jco]] - bAGT catalytic domain		[[1g8o]], [[1fg5]], [[2jco]] - bAGT catalytic domain
Line 83:		Line 72:

	[[1vzu]], [[1vzx]], [[2i7b]] - hAGT catalytic domain (mutant)		[[1vzu]], [[1vzx]], [[2i7b]] - hAGT catalytic domain (mutant)
-
-

	===AGT binary complexes===		===AGT binary complexes===
-
-

	[[1g93]], [[1gx0]] - bAGT catalytic domain + UDP-galactose		[[1g93]], [[1gx0]] - bAGT catalytic domain + UDP-galactose
Line 113:		Line 98:

	[[2a8u]], [[2a8w]] - hAGT catalytic domain + methyl lactoside		[[2a8u]], [[2a8w]] - hAGT catalytic domain + methyl lactoside
-
-

	===AGT ternary complexes===		===AGT ternary complexes===
-
-

	[[1gwv]] - bAGT catalytic domain + UDP + lactose		[[1gwv]] - bAGT catalytic domain + UDP + lactose
Line 139:		Line 120:

	[[1lzj]], [[1lzi]] - hAGT catalytic domain + UDP + H antigen acceptor		[[1lzj]], [[1lzi]] - hAGT catalytic domain + UDP + H antigen acceptor
-
-

	===Fucosylglycoprotein 3-AGT (GTB)===		===Fucosylglycoprotein 3-AGT (GTB)===
-
-

	[[2pgv]], [[2pgy]], [[2riz]], [[2rj5]], [[1wsz]], [[1wt0]], [[1wt1]], [[2o1f]], [[2o1g]], [[2y7a]], [[3i0c]], [[3i0h]], [[1xz6]] – hGTB (mutant)		[[2pgv]], [[2pgy]], [[2riz]], [[2rj5]], [[1wsz]], [[1wt0]], [[1wt1]], [[2o1f]], [[2o1g]], [[2y7a]], [[3i0c]], [[3i0h]], [[1xz6]] – hGTB (mutant)
Line 168:		Line 145:
	[[1zi1]] - hAGT catalytic domain + lactose<br />		[[1zi1]] - hAGT catalytic domain + lactose<br />
	[[1zi3]] - hAGT catalytic domain + acetylactosamine		[[1zi3]] - hAGT catalytic domain + acetylactosamine
-
-

	===Fucosylgalactoside α N-acetylgalactosaminyltrasferase (GTA)===		===Fucosylgalactoside α N-acetylgalactosaminyltrasferase (GTA)===
-
-

	[[3ioh]] – hGTA (mutant)		[[3ioh]] – hGTA (mutant)

Michal Harel at 08:33, 11 June 2012

2012-06-11T08:33:36Z

←Older revision		Revision as of 08:33, 11 June 2012
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	{{STRUCTURE_1fr8\| PDB=1fr8 \| SIZE=400\| SCENE= \|right\|CAPTION=Bovine b-1,4-galactosyltransferase catalytic domain complex with UDP-galactose, [[1fr8]] }}		{{STRUCTURE_1fr8\| PDB=1fr8 \| SIZE=400\| SCENE= \|right\|CAPTION=Bovine b-1,4-galactosyltransferase catalytic domain complex with UDP-galactose, [[1fr8]] }}

-	'''Galactosyltransferase''' (GT) catalyzes the transfer of galactose during the synthesis of polysaccharides. the synthesis of polysaccharides requires hundreds of different glycosyltransferases. In plants, the elongation of N-glycan chains involves the sequential addition of galactose and fucose residues by β-1,3-galactosyltransferase and α-1,4-fucosyltransferase (GTB). '''β-1,4-galactosyltransferase''' (BGT) transfers galactose in a β-1,4 linkage to acceptor sugars like GlcNac, Glc and Xyl. '''α-1,3-galactosyltransferase''' (AGT) synthesizes α-1,3-galactose epitopes which are the major xenoantigens causing hyperacute rejection in pit-to-human xenotransplantation. Fucosylglycoprotein 3-AGT (GTB) or histo-blood group ABO system transferase is the basis of the blood ABO system. It involves 3 carbohydrate antigens: A, B and H. Individuals with blood type A, B and AB has glycosyltransferase activitiy which converts antigen H to A or B. Individuals with blood type O lack this activity.	+	'''Galactosyltransferase''' (GT) catalyzes the transfer of galactose during the synthesis of polysaccharides. the synthesis of polysaccharides requires hundreds of different glycosyltransferases. In plants, the elongation of N-glycan chains involves the sequential addition of galactose and fucose residues by β-1,3-galactosyltransferase and α-1,4-fucosyltransferase (GTB). '''β-1,4-galactosyltransferase''' (BGT) transfers galactose in a β-1,4 linkage to acceptor sugars like GlcNac, Glc and Xyl. '''α-1,3-galactosyltransferase''' (AGT) synthesizes α-1,3-galactose epitopes which are the major xenoantigens causing hyperacute rejection in pit-to-human xenotransplantation. '''Fucosylglycoprotein 3-AGT''' (GTB) or histo-blood group ABO system transferase is the basis of the blood ABO system. It involves 3 carbohydrate antigens: A, B and H. Individuals with blood type A, B and AB has glycosyltransferase activitiy which converts antigen H to A or B. Individuals with blood type O lack this activity.
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Michal Harel at 08:33, 11 June 2012

2012-06-11T08:33:11Z

←Older revision		Revision as of 08:33, 11 June 2012
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	{{STRUCTURE_1fr8\| PDB=1fr8 \| SIZE=400\| SCENE= \|right\|CAPTION=Bovine b-1,4-galactosyltransferase catalytic domain complex with UDP-galactose, [[1fr8]] }}		{{STRUCTURE_1fr8\| PDB=1fr8 \| SIZE=400\| SCENE= \|right\|CAPTION=Bovine b-1,4-galactosyltransferase catalytic domain complex with UDP-galactose, [[1fr8]] }}

-	'''Galactosyltransferase''' (GT) catalyzes the transfer of galactose during the synthesis of polysaccharides. the synthesis of polysaccharides requires hundreds of different glycosyltransferases. In plants, the elongation of N-glycan chains involves the sequential addition of galactose and fucose residues by β-1,3-galactosyltransferase and α-1,4-fucosyltransferase (GTB). '''β-1,4-galactosyltransferase''' (BGT) transfers galactose in a β-1,4 linkage to acceptor sugars like GlcNac, Glc and Xyl. '''α-1,3-galactosyltransferase''' (AGT) synthesizes α-1,3-galactose epitopes which are the major xenoantigens causing hyperacute rejection in pit-to-human xenotransplantation.	+	'''Galactosyltransferase''' (GT) catalyzes the transfer of galactose during the synthesis of polysaccharides. the synthesis of polysaccharides requires hundreds of different glycosyltransferases. In plants, the elongation of N-glycan chains involves the sequential addition of galactose and fucose residues by β-1,3-galactosyltransferase and α-1,4-fucosyltransferase (GTB). '''β-1,4-galactosyltransferase''' (BGT) transfers galactose in a β-1,4 linkage to acceptor sugars like GlcNac, Glc and Xyl. '''α-1,3-galactosyltransferase''' (AGT) synthesizes α-1,3-galactose epitopes which are the major xenoantigens causing hyperacute rejection in pit-to-human xenotransplantation. Fucosylglycoprotein 3-AGT (GTB) or histo-blood group ABO system transferase is the basis of the blood ABO system. It involves 3 carbohydrate antigens: A, B and H. Individuals with blood type A, B and AB has glycosyltransferase activitiy which converts antigen H to A or B. Individuals with blood type O lack this activity.
-		+
	{{TOC limit\|limit=2}}		{{TOC limit\|limit=2}}

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	==3D structures of galactosyltransferase==		==3D structures of galactosyltransferase==

-	''Updated ~~November 2011~~''	+	''Updated June 2012''

	===β-1,4-galactosyltransferase (BGT)===		===β-1,4-galactosyltransferase (BGT)===
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	[[1r82]] - hAGT catalytic domain + UDP-galactose + inhibitor + fucose		[[1r82]] - hAGT catalytic domain + UDP-galactose + inhibitor + fucose

-	[[1lzj]] - hAGT catalytic domain + UDP + H antigen acceptor	+	[[1lzj]], [[1lzi]] - hAGT catalytic domain + UDP + H antigen acceptor


Line 147:		Line 146:


-	[[2pgv]], [[2pgy]], [[2riz]], [[2rj5]] – hGTB (mutant)	+	[[2pgv]], [[2pgy]], [[2riz]], [[2rj5]], [[1wsz]], [[1wt0]], [[1wt1]], [[2o1f]], [[2o1g]], [[2y7a]], [[3i0c]], [[3i0h]], [[1xz6]] – hGTB (mutant)

-	[[2rit]] – hGTB	+	[[2rit]], [[1lz0]] – hGTB

-	[[2rix]] – hGTB + UDP	+	[[2rix]] – hGTB + UDP<br />
-		+	[[2o1h]], [[3i0d]], [[3i0i]] – hGT Abo (mutant) + UDP<br />
-	[[2riy]] – hGTB + H-antigen acceptor	+	[[2riy]] – hGTB + H-antigen acceptor<br />
-		+	[[1zi4]], [[1zi5]] - hGTB + H-antigen<br />
-	[[2rj1]], [[2rj6]] - hGTB (mutant) + UDP + H-antigen disaccharide	+	[[3i0j]] – hGT Abo (mutant) + H-antigen<br />
		+	[[2rj1]], [[2rj6]], [[3i0e]], [[3i0f]], [[3i0g]], [[3i0k]], [[3l0l]] - hGTB (mutant) + UDP + H-antigen disaccharide

	[[2rj8]] - hGTB + UDP + H-antigen disaccharide		[[2rj8]] - hGTB + UDP + H-antigen disaccharide

-	[[2rj4]] - hGTB (mutant) + UDP + ADA	+	[[2rj4]] - hGTB (mutant) + UDP + ADA<br />
-		+	[[1wt2]] - hGTB (mutant) + UDP + inhibitor<br />
		+	[[1wt2]] - hGTB (mutant) + UDP + acceptor<br />
	[[2rj7]] - hGTB (mutant) + UDP-galactose + deoxy-acceptor		[[2rj7]] - hGTB (mutant) + UDP-galactose + deoxy-acceptor

-	[[2rj9]] - hGTB + UDP-galactose + deoxy-acceptor	+	[[2rj9]] - hGTB + UDP-galactose + deoxy-acceptor<br />
		+	[[3sx3]], [[3sx5]], [[3sx7]], [[3sx8]], [[3sxc]], [[3sxd]], [[3sxe]], [[3sxg]], [[1zhj]], [[1zjo]] - hAGT catalytic domain + galactose<br />
		+	[[1zi1]] - hAGT catalytic domain + lactose<br />
		+	[[1zi3]] - hAGT catalytic domain + acetylactosamine

Michal Harel at 06:56, 11 June 2012

2012-06-11T06:56:00Z

←Older revision		Revision as of 06:56, 11 June 2012
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	{{STRUCTURE_1fr8\| PDB=1fr8 \| SIZE=400\| SCENE= \|right\|CAPTION=Bovine b-1,4-galactosyltransferase catalytic domain complex with UDP-galactose, [[1fr8]] }}		{{STRUCTURE_1fr8\| PDB=1fr8 \| SIZE=400\| SCENE= \|right\|CAPTION=Bovine b-1,4-galactosyltransferase catalytic domain complex with UDP-galactose, [[1fr8]] }}

-	'''Galactosyltransferase''' (GT) catalyzes the transfer of galactose during the synthesis of polysaccharides. the synthesis of polysaccharides requires hundreds of different glycosyltransferases. In plants, the elongation of N-glycan chains involves the sequential addition of galactose and fucose residues by β-1,3-galactosyltransferase and α-1,4-fucosyltransferase (GTB). β-1,4-galactosyltransferase (BGT) transfers galactose in a β-1,4 linkage to acceptor sugars like GlcNac, Glc and Xyl. α-1,3-galactosyltransferase (AGT) synthesizes α-1,3-galactose epitopes which are the major xenoantigens causing hyperacute rejection in pit-to-human xenotransplantation.	+	'''Galactosyltransferase''' (GT) catalyzes the transfer of galactose during the synthesis of polysaccharides. the synthesis of polysaccharides requires hundreds of different glycosyltransferases. In plants, the elongation of N-glycan chains involves the sequential addition of galactose and fucose residues by β-1,3-galactosyltransferase and α-1,4-fucosyltransferase (GTB). '''β-1,4-galactosyltransferase''' (BGT) transfers galactose in a β-1,4 linkage to acceptor sugars like GlcNac, Glc and Xyl. '''α-1,3-galactosyltransferase''' (AGT) synthesizes α-1,3-galactose epitopes which are the major xenoantigens causing hyperacute rejection in pit-to-human xenotransplantation.

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Michal Harel at 08:02, 19 March 2012

2012-03-19T08:02:44Z

←Older revision		Revision as of 08:02, 19 March 2012
Line 2:		Line 2:
	{{STRUCTURE_1fr8\| PDB=1fr8 \| SIZE=400\| SCENE= \|right\|CAPTION=Bovine b-1,4-galactosyltransferase catalytic domain complex with UDP-galactose, [[1fr8]] }}		{{STRUCTURE_1fr8\| PDB=1fr8 \| SIZE=400\| SCENE= \|right\|CAPTION=Bovine b-1,4-galactosyltransferase catalytic domain complex with UDP-galactose, [[1fr8]] }}

-	'''Galactosyltransferase''' (GT) catalyzes the transfer of galactose during the synthesis of polysaccharides.	+	'''Galactosyltransferase''' (GT) catalyzes the transfer of galactose during the synthesis of polysaccharides. the synthesis of polysaccharides requires hundreds of different glycosyltransferases. In plants, the elongation of N-glycan chains involves the sequential addition of galactose and fucose residues by β-1,3-galactosyltransferase and α-1,4-fucosyltransferase (GTB). β-1,4-galactosyltransferase (BGT) transfers galactose in a β-1,4 linkage to acceptor sugars like GlcNac, Glc and Xyl. α-1,3-galactosyltransferase (AGT) synthesizes α-1,3-galactose epitopes which are the major xenoantigens causing hyperacute rejection in pit-to-human xenotransplantation.

	{{TOC limit\|limit=2}}		{{TOC limit\|limit=2}}

Michal Harel at 07:37, 19 March 2012

2012-03-19T07:37:14Z

←Older revision		Revision as of 07:37, 19 March 2012
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	[[2vs4]], [[2vs5]], [[2vxl]], [[2vxm]] - bAGT catalytic domain (mutant) + UDP-galactose		[[2vs4]], [[2vs5]], [[2vxl]], [[2vxm]] - bAGT catalytic domain (mutant) + UDP-galactose

-	[[2vs3 - bAGT catalytic domain + UDP-galactose	+	[[2vs3]] - bAGT catalytic domain + UDP-galactose

	[[1ziz]], [[1zjp]] - hAGT catalytic domain + galactose		[[1ziz]], [[1zjp]] - hAGT catalytic domain + galactose

Michal Harel at 09:56, 28 November 2011

2011-11-28T09:56:05Z

←Older revision		Revision as of 09:56, 28 November 2011
Line 8:		Line 8:

	==3D structures of galactosyltransferase==		==3D structures of galactosyltransferase==
		+
		+	''Updated November 2011''

	===β-1,4-galactosyltransferase (BGT)===		===β-1,4-galactosyltransferase (BGT)===
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	[[1k4v]] - bAGT catalytic domain + UDP		[[1k4v]] - bAGT catalytic domain + UDP
		+
		+	[[2rj0]] - bAGT catalytic domain + UDP + Mn

	[[1vzt]], [[2jcj]] - bAGT catalytic domain (mutant) + UDP		[[1vzt]], [[2jcj]] - bAGT catalytic domain (mutant) + UDP
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	[[3ioj]] - hGTA (mutant) + UDP		[[3ioj]] - hGTA (mutant) + UDP
		+
		+	===Various galactosyltransferase===
		+
		+	[[1g9r]] - GT (mutant) + UDP-galactose + Mn – ''Neisseria meningitidis''
		+
		+	[[2o1f]], [[2o1g]] – hGT ABO (mutant)
		+
		+	[[2o1h]] – hGT ABO (mutant) + UDP

	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 11:52, 26 October 2011

2011-10-26T11:52:25Z

←Older revision		Revision as of 11:52, 26 October 2011
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	[[1tw5]] - bBGT catalytic domain (mutant) + chitobiose		[[1tw5]] - bBGT catalytic domain (mutant) + chitobiose

-	[[1nf5]] - bBGT + a-lactalbumin	+	[[1nf5]] - bBGT + α-lactalbumin

	[[1o23]] - bBGT catalytic domain + UDP-glucose		[[1o23]] - bBGT catalytic domain + UDP-glucose
Line 47:		Line 47:


-	[[1nhe]], [[1nkh]] - bBGT catalytic domain + UDP + a-lactalbumin	+	[[1nhe]], [[1nkh]] - bBGT catalytic domain + UDP + α-lactalbumin

-	[[1yro]] - bBGT catalytic domain (mutant) + UDP-galactose + a-lactalbumin	+	[[1yro]] - bBGT catalytic domain (mutant) + UDP-galactose + α-lactalbumin

-	[[1nwg]] - bBGT catalytic domain + N-butanoyl-glucoamine + a-lactalbumin	+	[[1nwg]] - bBGT catalytic domain + N-butanoyl-glucoamine + α-lactalbumin

	[[1nmm]], [[1nqi]], [[1pzy]], [[1o7q]] - bBGT catalytic domain + UDP + GlcNAc		[[1nmm]], [[1nqi]], [[1pzy]], [[1o7q]] - bBGT catalytic domain + UDP + GlcNAc
Line 61:		Line 61:
	[[2aec]], [[2aes]], [[2agd]] - hBGT catalytic domain + UDP + GlcNAc + mannose		[[2aec]], [[2aes]], [[2agd]] - hBGT catalytic domain + UDP + GlcNAc + mannose

-	[[2fyc]] - hBGT catalytic domain (mutant) + UDP-galactose + Ca + a-lactalbumin	+	[[2fyc]] - hBGT catalytic domain (mutant) + UDP-galactose + Ca + α-lactalbumin

-	[[3ee5]] - hBGT catalytic domain (mutant) + UDP + GlcNAc-b1,3-gal-b-naphthalenemethanol	+	[[3ee5]] - hBGT catalytic domain (mutant) + UDP + GlcNAc-β1,3-gal-β-naphthalenemethanol

-	[[2fyd]] - hBGT catalytic domain + UDP-GlcNAc + Mn + a-lactalbumin (mutant) + glucose	+	[[2fyd]] - hBGT catalytic domain + UDP-GlcNAc + Mn + α-lactalbumin (mutant) + glucose

	[[2ah9]] - hBGT catalytic domain + UDP + chitotriose		[[2ah9]] - hBGT catalytic domain + UDP + chitotriose