Kdo-8-phosphate synthase - Revision history

Michal Harel at 08:35, 11 October 2021

2021-10-11T08:35:54Z

←Older revision		Revision as of 08:35, 11 October 2021
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	*Non-metallo Kdo8P		*Non-metallo Kdo8P

-	**[[1d9e]], [[1gg0]], [[1x8f]] – EcKPS - ''Escherichia coli''<br />	+	**[[1d9e]], [[1gg0]], [[1x8f]], [[6u57]] – EcKPS - ''Escherichia coli''<br />
	**[[1o60]] - KPS – ''Haemophilus influenza''<br />		**[[1o60]] - KPS – ''Haemophilus influenza''<br />
	**[[3e9a]] - KPS – ''Vibrio cholerae''<br />		**[[3e9a]] - KPS – ''Vibrio cholerae''<br />
	**[[3fs2]] - KPS – ''Brucella melitensis''<br />		**[[3fs2]] - KPS – ''Brucella melitensis''<br />
-	**[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria''	+	**[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria''<br />
		+	**[[6mdy]] – KPS – ''Legionella pneumophila''<br />

	*''Non-metallo Kdo8P binary complex''		*''Non-metallo Kdo8P binary complex''

Michal Harel at 08:21, 11 October 2021

2021-10-11T08:21:32Z

←Older revision		Revision as of 08:21, 11 October 2021
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	== Function ==		== Function ==
-	'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.	+	'''Kdo-8-phosphate synthase''' or '''2-dehydro-3-deoxyphosphooctonate aldolase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.

	== Structural highlights ==		== Structural highlights ==

Alexander Berchansky at 10:40, 1 July 2019

2019-07-01T10:40:06Z

←Older revision		Revision as of 10:40, 1 July 2019
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	== Structural highlights ==		== Structural highlights ==
-	The biological assembly of Metallo Kdo-8-phosphate synthase from ''Neisseria meningitidis'' is <scene name='52/525184/Cv/3'>homotetramer</scene>. The <scene name='52/525184/Cv/6'>active site of the metallo KPS contains a divalent cation and the PEP substrate</scene><ref>PMID:19447118</ref>. Water molecules shown as red spheres.	+	The biological assembly of Metallo Kdo-8-phosphate synthase from ''Neisseria meningitidis'' is <scene name='52/525184/Cv/7'>homotetramer</scene>. The <scene name='52/525184/Cv/8'>active site of the metallo KPS contains a divalent cation and the PEP substrate</scene><ref>PMID:19447118</ref>. Water molecules shown as red spheres.
	</StructureSection>		</StructureSection>
	==3D structures of Kdo-8-phosphate synthase==		==3D structures of Kdo-8-phosphate synthase==

Joel L. Sussman at 07:02, 25 July 2018

2018-07-25T07:02:37Z

←Older revision		Revision as of 07:02, 25 July 2018
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-	<StructureSection load='~~3fyp~~' size='350' side='right' scene='52/525184/Cv/2' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>	+	<StructureSection load='' size='350' side='right' scene='52/525184/Cv/2' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>

	== Function ==		== Function ==

Michal Harel at 09:35, 24 July 2018

2018-07-24T09:35:53Z

←Older revision		Revision as of 09:35, 24 July 2018
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-	<StructureSection load='3fyp' size='~~450~~' side='right' scene='52/525184/Cv/2' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>	+	<StructureSection load='3fyp' size='350' side='right' scene='52/525184/Cv/2' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>

	== Function ==		== Function ==
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	**[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br />		**[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br />
	**[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br />		**[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br />
		+	**[[4z1a]] – HpKPS – ''Helicobacter pylori''<br />
		+	**[[4z1b]] - HpKPS (mutant)<br />
		+	**[[4z1c]] - HpKPS + Cd<br />
		+	**[[6bng]] – KPS – ''Acinetobacter baumannii''<br />

	*''Metallo Kdo8P binary complex''		*''Metallo Kdo8P binary complex''
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	**[[3e12]] - AaKPS + Kdo-8-P + Cu<br />		**[[3e12]] - AaKPS + Kdo-8-P + Cu<br />
	**[[2nxi]], [[1fwn]] - AaKPS + PEP<br />		**[[2nxi]], [[1fwn]] - AaKPS + PEP<br />
-	**[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br />	+	**[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br />
		+	**[[4z1d]] - HpKPS + PEP + Zn<br />
	**[[3fyp]] - NmKPS (mutant) + PEP + Mn<br />		**[[3fyp]] - NmKPS (mutant) + PEP + Mn<br />
	**[[3tmq]] - BpKPS + arabinose-5-phosphate<br />		**[[3tmq]] - BpKPS + arabinose-5-phosphate<br />

Alexander Berchansky at 10:14, 14 April 2016

2016-04-14T10:14:39Z

←Older revision		Revision as of 10:14, 14 April 2016
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	== Structural highlights ==		== Structural highlights ==
-	The biological assembly of Metallo Kdo-8-phosphate synthase from ''Neisseria meningitidis'' is <scene name='52/525184/Cv/3'>homotetramer</scene>. The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>.	+	The biological assembly of Metallo Kdo-8-phosphate synthase from ''Neisseria meningitidis'' is <scene name='52/525184/Cv/3'>homotetramer</scene>. The <scene name='52/525184/Cv/6'>active site of the metallo KPS contains a divalent cation and the PEP substrate</scene><ref>PMID:19447118</ref>. Water molecules shown as red spheres.
	</StructureSection>		</StructureSection>
	==3D structures of Kdo-8-phosphate synthase==		==3D structures of Kdo-8-phosphate synthase==

Alexander Berchansky at 14:33, 13 April 2016

2016-04-13T14:33:26Z

←Older revision		Revision as of 14:33, 13 April 2016
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-	<StructureSection load='3fyp' size='450' side='right' scene='' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>	+	<StructureSection load='3fyp' size='450' side='right' scene='52/525184/Cv/2' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>

	== Function ==		== Function ==
Line 5:		Line 5:

	== Structural highlights ==		== Structural highlights ==
-	The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>.	+	The biological assembly of Metallo Kdo-8-phosphate synthase from ''Neisseria meningitidis'' is <scene name='52/525184/Cv/3'>homotetramer</scene>. The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>.
	</StructureSection>		</StructureSection>
	==3D structures of Kdo-8-phosphate synthase==		==3D structures of Kdo-8-phosphate synthase==

Alexander Berchansky at 14:23, 13 April 2016

2016-04-13T14:23:20Z

←Older revision		Revision as of 14:23, 13 April 2016
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-	~~{{STRUCTURE_3fyp\| PDB~~=3fyp ~~\| SIZE~~=~~400\| SCENE~~= \|right~~\|CAPTION~~=Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]] }}	+	<StructureSection load='3fyp' size='450' side='right' scene='' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>
		+
	== Function ==		== Function ==
	'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.		'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.
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	== Structural highlights ==		== Structural highlights ==
	The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>.		The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>.
-		+	</StructureSection>
	==3D structures of Kdo-8-phosphate synthase==		==3D structures of Kdo-8-phosphate synthase==
	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}		Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}

Michal Harel at 10:22, 6 April 2016

2016-04-06T10:22:21Z

←Older revision		Revision as of 10:22, 6 April 2016
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	{{STRUCTURE_3fyp\| PDB=3fyp \| SIZE=400\| SCENE= \|right\|CAPTION=Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]] }}		{{STRUCTURE_3fyp\| PDB=3fyp \| SIZE=400\| SCENE= \|right\|CAPTION=Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]] }}
	== Function ==		== Function ==
-	'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). Some bacteria like 'Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.	+	'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.

	== Structural highlights ==		== Structural highlights ==

Michal Harel at 10:21, 6 April 2016

2016-04-06T10:21:51Z

←Older revision		Revision as of 10:21, 6 April 2016
Line 1:		Line 1:
	{{STRUCTURE_3fyp\| PDB=3fyp \| SIZE=400\| SCENE= \|right\|CAPTION=Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]] }}		{{STRUCTURE_3fyp\| PDB=3fyp \| SIZE=400\| SCENE= \|right\|CAPTION=Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]] }}
	== Function ==		== Function ==
-	'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). '''Metallo KPS''' is a metal-requiring enzyme while '''Non-metallo KPS''' does not require a divalent cation for its activity.	+	'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). Some bacteria like 'Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.
-	Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.	+

	== Structural highlights ==		== Structural highlights ==
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	**[[1fxp]] - AaKPS + Cd – ''Aquifex aeolicus''<br />		**[[1fxp]] - AaKPS + Cd – ''Aquifex aeolicus''<br />
	**[[1lrn]], [[1t99]] - AaKPS (mutant) + Cd<br />		**[[1lrn]], [[1t99]] - AaKPS (mutant) + Cd<br />
		+	**[[1fx6]] - AaKPS <br />
		+	**[[2nws]], [[2nx3]] - AaKPS (mutant) <br />
	**[[2qkf]] – NmKPS + Na – ''Neisseria meningitides''<br />		**[[2qkf]] – NmKPS + Na – ''Neisseria meningitides''<br />
	**[[3fyo]] - NmKPS (mutant) + Mn<br />		**[[3fyo]] - NmKPS (mutant) + Mn<br />
	**[[3qpy]], [[3qpz]], [[3qq0]], [[3stc]], [[3ste]], [[3stf]], [[4jte]], [[4jtf]], [[4jtg]], [[4jth]], [[4jti]], [[4jtj]], [[4jtk]], [[4jtl]] - NmKPS (mutant) + Na<br />		**[[3qpy]], [[3qpz]], [[3qq0]], [[3stc]], [[3ste]], [[3stf]], [[4jte]], [[4jtf]], [[4jtg]], [[4jth]], [[4jti]], [[4jtj]], [[4jtk]], [[4jtl]] - NmKPS (mutant) + Na<br />
		+	**[[3qq1]], [[3stg]] - NmKPS (mutant) <br />
	**[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br />		**[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br />
	**[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br />		**[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br />
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	**[[1pck]] - AaKPS + PEP derivative + Cd<br />		**[[1pck]] - AaKPS + PEP derivative + Cd<br />
	**[[1pe1]] - AaKPS + phosphoglyceric acid + Cd<br />		**[[1pe1]] - AaKPS + phosphoglyceric acid + Cd<br />
-	**[[2a21]] - ~~EcKPS~~ + PEP + Zn ~~- ''Escherichia coli''~~<br />	+	**[[2a21]] - AaKPS + PEP + Zn <br />
	**[[1jcx]], [[1pcw]] - AaKPS + inhibitor + Cd<br />		**[[1jcx]], [[1pcw]] - AaKPS + inhibitor + Cd<br />
	**[[1fy6]] - AaKPS + A5P + Cd<br />		**[[1fy6]] - AaKPS + A5P + Cd<br />
	**[[3e12]] - AaKPS + Kdo-8-P + Cu<br />		**[[3e12]] - AaKPS + Kdo-8-P + Cu<br />
		+	**[[2nxi]], [[1fwn]] - AaKPS + PEP<br />
		+	**[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br />
	**[[3fyp]] - NmKPS (mutant) + PEP + Mn<br />		**[[3fyp]] - NmKPS (mutant) + PEP + Mn<br />
	**[[3tmq]] - BpKPS + arabinose-5-phosphate<br />		**[[3tmq]] - BpKPS + arabinose-5-phosphate<br />
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	**[[2a2i]] - AaKPS + A5P + PEP + Zn<br />		**[[2a2i]] - AaKPS + A5P + PEP + Zn<br />
	**[[1lrq]], [[1t8x]] - AaKPS (mutant) + A5P + PEP + Cd<br />		**[[1lrq]], [[1t8x]] - AaKPS (mutant) + A5P + PEP + Cd<br />
		+	**[[1fxq]] - AaKPS + A5P + PEP<br />
		+	**[[2nx1]], [[2ef9]] - AaKPS (mutant) + A5P + PEP<br />
		+	**[[2nxg]] - AaKPS (mutant) + octanoic acid derivative + A5P + PEP<br />
	**[[3und]] - BpKPS + arabinose-5-phosphate + PEP<br />		**[[3und]] - BpKPS + arabinose-5-phosphate + PEP<br />

	*Non-metallo Kdo8P		*Non-metallo Kdo8P

-	**[[1d9e]], [[1gg0]], [[1x8f]] – EcKPS ~~<br />~~	+	**[[1d9e]], [[1gg0]], [[1x8f]] – EcKPS - ''Escherichia coli''<br />
-	**[[1fx6]] - ~~AaKPS <br />~~	+
-	**[[2nws]], [[2nx3]] - AaKPS (mutant) <br />	+
	**[[1o60]] - KPS – ''Haemophilus influenza''<br />		**[[1o60]] - KPS – ''Haemophilus influenza''<br />
	**[[3e9a]] - KPS – ''Vibrio cholerae''<br />		**[[3e9a]] - KPS – ''Vibrio cholerae''<br />
	**[[3fs2]] - KPS – ''Brucella melitensis''<br />		**[[3fs2]] - KPS – ''Brucella melitensis''<br />
-	**[[3qq1]], [[3stg]] - NmKPS (mutant) <br />
	**[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria''		**[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria''

	*''Non-metallo Kdo8P binary complex''		*''Non-metallo Kdo8P binary complex''

-	**[[2nxi]], [[1fwn]] - AaKPS + PEP<br />
-	**[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br />
	**[[1g7u]], [[1q3n]] - EcKPS + PEP<br />		**[[1g7u]], [[1q3n]] - EcKPS + PEP<br />
	**[[1phq]], [[1pl9]] - EcKPS + PEP derivative<br />		**[[1phq]], [[1pl9]] - EcKPS + PEP derivative<br />
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	**[[1x6u]] - EcKPS + Kdo-8-P		**[[1x6u]] - EcKPS + Kdo-8-P

-	*''Non-metallo Kdo8P ternary complex''
-
-	**[[1fxq]] - AaKPS + A5P + PEP<br />
-	**[[2nx1]], [[2ef9]] - AaKPS (mutant) + A5P + PEP<br />
-	**[[2nxg]] - AaKPS (mutant) + octanoic acid derivative + A5P + PEP<br />
	}}		}}
	== References ==		== References ==
	<references/>		<references/>
	[[Category:Topic Page]]		[[Category:Topic Page]]