Michal Harel at 06:32, 11 July 2011

2011-07-11T06:32:25Z

←Older revision		Revision as of 06:32, 11 July 2011
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-	{{STRUCTURE_1yrs \| PDB=1yrs \| SCENE= }}	+	{{STRUCTURE_1yrs\| PDB=1yrs \| SIZE=300\| SCENE= \|right\| CAPTION=Kinesin-5 complex with inhibitor, ADP and Mg+2 ion, [[1yrs]] }}
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	==Function==		==Function==

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	Note: Most of the analysis is derived from 1YRS.pdb.		Note: Most of the analysis is derived from 1YRS.pdb.
	[[Image:1YRS_consurf.png\|center\|250px\|Conserved residues of 1YRS.pdb evaluated by ConSurf. Red are most conserved, blue least conserved.]]		[[Image:1YRS_consurf.png\|center\|250px\|Conserved residues of 1YRS.pdb evaluated by ConSurf. Red are most conserved, blue least conserved.]]
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		+	==3D structures of kinesin==
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		+	[[Kinesin]]

	==References==		==References==

Shiela M. Jones: New page: {{STRUCTURE_1yrs | PDB=1yrs | SCENE= }} ==Function== Kinesins are an [http://en.wikipedia.org/wiki/ATPase ATPase] motor protein found in Eukaryotic cells. Kinesins support [http://en...

2008-12-09T10:13:24Z

New page: {{STRUCTURE_1yrs | PDB=1yrs | SCENE= }} ==Function== Kinesins are an [http://en.wikipedia.org/wiki/ATPase ATPase] motor protein found in Eukaryotic cells. Kinesins support [http://en...

New page

{{STRUCTURE_1yrs | PDB=1yrs | SCENE= }}
==Function==

Kinesins are an [http://en.wikipedia.org/wiki/ATPase ATPase] motor protein found in Eukaryotic cells. Kinesins support [http://en.wikipedia.org/wiki/Mitosis mitosis], [http://en.wikipedia.org/wiki/Meiosis meiosis] and transport cellular cargo along microtubule cables inside the cell. Kinesin-5 is a dimer-of-dimers with two motor domains, located at the end of a four-stranded stalk.

==Structure==

Kinesin-5 is a dimer-of-dimers with two major domains; the motor domain, a globular region that binds to the microtubules, and the stalk, a long coiled-coil tail which binds to the cargo.
[[Image:1YRS_Compare.png|center|250px|1YRS.pdb.]]

The concavity of the motor domain, shown below, can be used to determine possible binding spots. The most likely spot, determined with [http://hotpatch.mbi.ucla.edu/ HotPatch],
[[Image:1YRS_Concavity.png|center|250px|Concavity of 1YRS.pdb. Red is very concave, whereas blue is not concave. Produced with HotPatch.]]

It can be noted that immediately surrounding the main binding site of the motor domain are two mobile loops. Coloring the cartoon by occupancy (B-factor) can give a general idea of the mobility of the loops. As expected, the center of the protein is very well defined, shown in a dark to medium blue. The outside edges and loops, shown in red, indicate mobile residues with the possibility of many rotomers.
[[Image:1YRS_occupancy.png|center|250px|Occupancy of 1YRS.pdb. Dark blue residues are well-defined, whereas red are very mobile. Produced with PyMol.]]

Conserved residues for this protein was analyzed with ConSurf. Compared against 200 unique sequences, most conserved residues can be seen as a magenta, and least conserved as blue.
Note: Most of the analysis is derived from 1YRS.pdb.
[[Image:1YRS_consurf.png|center|250px|Conserved residues of 1YRS.pdb evaluated by ConSurf. Red are most conserved, blue least conserved.]]

==References==

[1] Kaseda, K.; Crevel, I.; Hirose, K.; Cross, R. A. “Single-headed mode of kinesin-5.” Euro. Mol. Bio. Org. reports. 2008, 9(8), 761-765.

[2] Kull, F. J.; Sablin, E. P.; Lau, R.; Fletterick, R. J.; Vale, R. D. “Crystal structure of the kinesin motor domain reveals a structural similarity to myosin.” Nature. 1996, 380, 550-555.

[3] Cox, C. D.; Breslin, M. J.; Mariano, B. J.; Coleman, P. J.; Buser, C. A.; et al. “Kinesin spindle protein (KSP) inhibitors. Part 1: The discovery of 3,5-diaryl-4,5-dihydropyrazoles as potent and selective inhibitors of the mitotic kinesin KSP.” Bioorg. & Med. Chem. Letters. 2005, 15, 2041-2045.

[4] Saunders, A. M.; Powers, J.; Strome, S.; Saxton, W. M. “Kinesin-5 acts as a brake in anaphase spindle elongation.” Current Biol. 2007, 17(12), 453-454.

Kinesin-5 - Revision history

Michal Harel at 06:32, 11 July 2011

Shiela M. Jones: New page: {{STRUCTURE_1yrs | PDB=1yrs | SCENE= }} ==Function== Kinesins are an [http://en.wikipedia.org/wiki/ATPase ATPase] motor protein found in Eukaryotic cells. Kinesins support [http://en...