http://52.214.119.220/wiki/index.php?action=history&feed=atom&title=Lambda_repressor 2026-04-11T03:57:56Z Revision history for this page on the wiki MediaWiki 1.11.2 http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=2572033&oldid=prev 2016-02-11T10:17:35Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:17, 11 February 2016</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&lt;StructureSection load=3bdn size='<del style="color: red; font-weight: bold; text-decoration: none;">450</del>' side='right' scene='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3' caption='Lambda repressor cI protein dimer complex with DNA (PDB code [[3bdn]])'&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&lt;StructureSection load=3bdn size='<ins style="color: red; font-weight: bold; text-decoration: none;">350</ins>' side='right' scene='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3' caption='Lambda repressor cI protein dimer complex with DNA (PDB code [[3bdn]])'&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=1823281&oldid=prev 2013-07-21T10:03:12Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:03, 21 July 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 24:</td> <td colspan="2" class="diff-lineno">Line 24:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 3D Structures of lambda repressor ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 3D Structures of lambda repressor ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">See </ins>[[<ins style="color: red; font-weight: bold; text-decoration: none;">Bacteriophage repressor</ins>]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[<del style="color: red; font-weight: bold; text-decoration: none;">1lrp</del>]] <del style="color: red; font-weight: bold; text-decoration: none;">– LR Cro protein – Enterobacteria phage λ &lt;br /&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[2hnf]], [[2ho0]] – LR (mutant) &lt;br /&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[1f39]] – LR cI protein C terminal &lt;br /&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[1lmb]], [[1lli]] – LR + DNA &lt;br /&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[3bdn]] – LR cI protein (mutant) + DNA&lt;br /&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=1823278&oldid=prev 2013-07-21T08:31:57Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 08:31, 21 July 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&lt;StructureSection load=3bdn size='450' side='right' scene='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3' caption='Lambda repressor dimer complex with DNA (PDB code [[3bdn]])'&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&lt;StructureSection load=3bdn size='450' side='right' scene='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3' caption='Lambda repressor <ins style="color: red; font-weight: bold; text-decoration: none;">cI protein </ins>dimer complex with DNA (PDB code [[3bdn]])'&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=1823277&oldid=prev 2013-07-21T08:31:13Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 08:31, 21 July 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 2:</td> <td colspan="2" class="diff-lineno">Line 2:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''cI''' is a transcription inhibitor of bacteriophage Lambda. Also known as '''Lambda Repressor''', cI is responsible for maintaining the lysogenic life cycle of phage Lambda. This is achieved when two repressor dimers bind cooperatively to adjacent operator sites on the DNA. The cooperative binding induces repression of the ''cro'' gene and simultaneous activation of the ''cI'' gene, which code for proteins Cro and cI, respectively (Stayrook et. al, 2008). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''cI''' is a transcription inhibitor of bacteriophage Lambda. Also known as '''Lambda Repressor''', cI is responsible for maintaining the lysogenic life cycle of phage Lambda. This is achieved when two repressor dimers bind cooperatively to adjacent operator sites on the DNA. The cooperative binding induces repression of the ''cro'' gene and simultaneous activation of the ''cI'' gene, which code for proteins <ins style="color: red; font-weight: bold; text-decoration: none;">'''</ins>Cro<ins style="color: red; font-weight: bold; text-decoration: none;">''' </ins>and <ins style="color: red; font-weight: bold; text-decoration: none;">'''</ins>cI<ins style="color: red; font-weight: bold; text-decoration: none;">'''</ins>, respectively (Stayrook et. al, 2008). </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Structural Overview==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Structural Overview==</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 26:</td> <td colspan="2" class="diff-lineno">Line 26:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[1lrp]] – LR <del style="color: red; font-weight: bold; text-decoration: none;"> </del>– Enterobacteria phage λ &lt;br /&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[1lrp]] – LR <ins style="color: red; font-weight: bold; text-decoration: none;">Cro protein </ins>– Enterobacteria phage λ &lt;br /&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2hnf]], [[2ho0]] – LR (mutant) &lt;br /&gt;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[2hnf]], [[2ho0]] – LR (mutant) &lt;br /&gt;</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[1f39]] – LR C terminal &lt;br /&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[1f39]] – LR <ins style="color: red; font-weight: bold; text-decoration: none;">cI protein </ins>C terminal &lt;br /&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[1lmb]], [[1lli]] – LR + DNA &lt;br /&gt;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[1lmb]], [[1lli]] – LR + DNA &lt;br /&gt;</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[3bdn]] – LR (mutant) + DNA&lt;br /&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[3bdn]] – LR <ins style="color: red; font-weight: bold; text-decoration: none;">cI protein </ins>(mutant) + DNA&lt;br /&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=1820482&oldid=prev 2013-07-14T10:30:17Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:30, 14 July 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&lt;StructureSection load=3bdn size='450' side='right' scene='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3' caption='(PDB code [[3bdn]])'&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&lt;StructureSection load=3bdn size='450' side='right' scene='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3' caption='<ins style="color: red; font-weight: bold; text-decoration: none;">Lambda repressor dimer complex with DNA </ins>(PDB code [[3bdn]])'&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=1820481&oldid=prev 2013-07-14T10:28:43Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:28, 14 July 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&lt;StructureSection load=3bdn size='450' side='right' scene='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3' caption=''&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&lt;StructureSection load=3bdn size='450' side='right' scene='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3' caption='<ins style="color: red; font-weight: bold; text-decoration: none;">(PDB code [[3bdn]])</ins>'&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td></tr> </table>

Michal Harel http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=1783807&oldid=prev 2013-04-24T13:14:24Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:14, 24 April 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 18:</td> <td colspan="2" class="diff-lineno">Line 18:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==N-Terminal Domain (NTD)==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==N-Terminal Domain (NTD)==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">&lt;StructureSection load='3bdn' size='350' side='right' caption='</del>The <del style="color: red; font-weight: bold; text-decoration: none;">spacefill model of the </del>NTD <del style="color: red; font-weight: bold; text-decoration: none;">highlights the interactions between the </del>repressor <del style="color: red; font-weight: bold; text-decoration: none;">and DNA. </del>(PDB entry [[1lmb]])<del style="color: red; font-weight: bold; text-decoration: none;">' scene='Bacteriophage_Lambda_Repressor_cI/Ntd_spacefill/1'&gt;The NTD Lambda repressor </del>consists of the first 92 amino acid residues of the protein and contains the &lt;scene name='Bacteriophage_Lambda_Repressor_cI/Ntd_spacefill/1'&gt;DNA-Binding Domain&lt;/scene&gt; (Stayrook et. al, 2008) of the protein. The secondary structure of the NTD is a compact conformation of six alpha-helices. This secondary structure shows weak self-association, which is purported to aid in formation of the dimeric unit. The DNA-binding motif utilized by Lambda Repressor is a &lt;scene name='Bacteriophage_Lambda_Repressor_cI/Hth_motif/1'&gt;Helix-Turn-Helix&lt;/scene&gt; (Beamer and Pabo, 1992). The Helix-Turn-Helix motifs of two dimerized NTDs are illustrated in red to the right.&lt;/StructureSection&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&#160;</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The NTD <ins style="color: red; font-weight: bold; text-decoration: none;">Lambda </ins>repressor (PDB entry [[1lmb]]) consists of the first 92 amino acid residues of the protein and contains the &lt;scene name='Bacteriophage_Lambda_Repressor_cI/Ntd_spacefill/1'&gt;DNA-Binding Domain&lt;/scene&gt; (Stayrook et. al, 2008) of the protein. The secondary structure of the NTD is a compact conformation of six alpha-helices. This secondary structure shows weak self-association, which is purported to aid in formation of the dimeric unit. The DNA-binding motif utilized by Lambda Repressor is a &lt;scene name='Bacteriophage_Lambda_Repressor_cI/Hth_motif/1'&gt;Helix-Turn-Helix&lt;/scene&gt; (Beamer and Pabo, 1992). The Helix-Turn-Helix motifs of two dimerized NTDs are illustrated in red to the right.</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&lt;/StructureSection&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 3D Structures of lambda repressor ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 3D Structures of lambda repressor ==</div></td></tr> </table>

Alexander Berchansky http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=1783806&oldid=prev 2013-04-24T13:11:10Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:11, 24 April 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 14:</td> <td colspan="2" class="diff-lineno">Line 14:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Connecting Region==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Connecting Region==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&lt;<del style="color: red; font-weight: bold; text-decoration: none;">StructureSection load='3bdn' size='350' side='right' caption='The CSR, consisting of two residues (Ala 111 and Gly 112), is highlighted in red. Cleavage occurs at the peptide bond between these residues. (PDB entry [[2hnf]])' </del>scene='Bacteriophage_Lambda_Repressor_cI/Csr/2'&gt;<del style="color: red; font-weight: bold; text-decoration: none;">The </del>region connecting the NTD to the CTD consists of 38 amino acid residues (residues 93-131) and contains the CSR. The CSR is on a long loop (residues 106-126) which serves to stabilize the homodimer through interactions with the connecting region of the opposite monomer. Cleavage occurs between Ala 111 and Gly 112 in the CSR. The natural structural conformation of the repressor is such that the auto-cleavage active site does not contact the CSR. During bacterial stress response, RecA binds to the repressor which induces a conformational change, bringing the CSR into contact with the auto-cleavage active site residues (Ndjonka et. al, 2006).<del style="color: red; font-weight: bold; text-decoration: none;">&lt;/StructureSection&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The </ins>&lt;scene <ins style="color: red; font-weight: bold; text-decoration: none;">name</ins>='Bacteriophage_Lambda_Repressor_cI/Csr/2'&gt;region connecting the NTD to the CTD<ins style="color: red; font-weight: bold; text-decoration: none;">&lt;/scene&gt; </ins>consists of 38 amino acid residues (residues 93-131) and contains the CSR <ins style="color: red; font-weight: bold; text-decoration: none;">([[2hnf]])</ins>. The CSR is on a long loop (residues 106-126) which serves to stabilize the homodimer through interactions with the connecting region of the opposite monomer. Cleavage occurs between Ala 111 and Gly 112 in the CSR. The natural structural conformation of the repressor is such that the auto-cleavage active site does not contact the CSR. During bacterial stress response, RecA binds to the repressor which induces a conformational change, bringing the CSR into contact with the auto-cleavage active site residues (Ndjonka et. al, 2006). </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==N-Terminal Domain (NTD)==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==N-Terminal Domain (NTD)==</div></td></tr> </table>

Alexander Berchansky http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=1783805&oldid=prev 2013-04-24T13:08:11Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:08, 24 April 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 10:</td> <td colspan="2" class="diff-lineno">Line 10:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==C-Terminal Domain (CTD)==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==C-Terminal Domain (CTD)==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&lt;<del style="color: red; font-weight: bold; text-decoration: none;">StructureSection load='3bdn' size='350' side='left' caption='Dimerized CTDs of two monomers. Note interactions between knotted β-sheets at the dimer-interface. Auto-cleavage active site residues are in yellow. (PDB entry [[1f39]])' </del>scene='Bacteriophage_Lambda_Repressor_cI/Ctd_dimer/1'&gt;<del style="color: red; font-weight: bold; text-decoration: none;">The </del>CTD of Lambda Repressor assumes a structural conformation similar to a knotted β-sheet and is composed of 104 amino acid resides (residues 132-236). This conformation is key in establishing the homodimer-forming interaction with the CTD of the opposite monomer. The CTD also facilitates the dimer-dimer interaction necessary for cooperative-binding repression. The &lt;scene name='Bacteriophage_Lambda_Repressor_cI/Cleavage_site_and_active_site/2'&gt;active site&lt;/scene&gt; of the auto-cleavage mechanism of Lambda Repressor is on the CTD. Two amino acid residues mediate the auto-cleavage activity of the repressor, Lys 192 and Ser 149 (Stayrook et. al, 2008).<del style="color: red; font-weight: bold; text-decoration: none;">&lt;/StructureSection&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The </ins>&lt;scene <ins style="color: red; font-weight: bold; text-decoration: none;">name</ins>='Bacteriophage_Lambda_Repressor_cI/Ctd_dimer/1'&gt;CTD of Lambda Repressor<ins style="color: red; font-weight: bold; text-decoration: none;">&lt;/scene&gt; </ins>assumes a structural conformation similar to a knotted β-sheet and is composed of 104 amino acid resides (residues 132-236<ins style="color: red; font-weight: bold; text-decoration: none;">, PDB entry [[1f39]]</ins>). This conformation is key in establishing the homodimer-forming interaction with the CTD of the opposite monomer. The CTD also facilitates the dimer-dimer interaction necessary for cooperative-binding repression. The &lt;scene name='Bacteriophage_Lambda_Repressor_cI/Cleavage_site_and_active_site/2'&gt;active site&lt;/scene&gt; of the auto-cleavage mechanism of Lambda Repressor is on the CTD. Two amino acid residues mediate the auto-cleavage activity of the repressor, Lys 192 and Ser 149 (Stayrook et. al, 2008). </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Connecting Region==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Connecting Region==</div></td></tr> </table>

Alexander Berchansky http://52.214.119.220/wiki/index.php?title=Lambda_repressor&diff=1783804&oldid=prev 2013-04-24T13:02:53Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:02, 24 April 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">{{STRUCTURE_3bdn| PDB</del>=3bdn <del style="color: red; font-weight: bold; text-decoration: none;"> | SCENE</del>='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3'<del style="color: red; font-weight: bold; text-decoration: none;">}}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">&lt;StructureSection load</ins>=3bdn <ins style="color: red; font-weight: bold; text-decoration: none;">size='450' side='right' scene</ins>='Bacteriophage_Lambda_Repressor_cI/Homodimer_bound_to_dna/3' <ins style="color: red; font-weight: bold; text-decoration: none;">caption=''&gt;</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Introduction ==</div></td></tr> </table>

Alexander Berchansky