Priyanka Basak/Sandbox - Revision history

http://52.214.119.220/wiki/index.php?action=history&feed=atom&title=Priyanka_Basak%2FSandbox Priyanka Basak/Sandbox - Revision history 2026-04-14T19:26:36Z Revision history for this page on the wiki MediaWiki 1.11.2 http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071861&oldid=prev Priyanka Basak at 20:40, 2 December 2014 2014-12-02T20:40:51Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:40, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Introduction==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Introduction==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><StructureSection load='3tj8' size='340' side='right' caption='UreE' scene=''></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><StructureSection load='3tj8' size='340' side='right' caption=<ins style="color: red; font-weight: bold; text-decoration: none;">'''Hp'</ins>'UreE<ins style="color: red; font-weight: bold; text-decoration: none;">,PDB ID: 1TJ8 </ins>' scene=''></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>UreE is an essential metallochaeprone for ''Helicobacter pylori'' (''H.pylori''). ''H.pylori'' is a class I human pathogen that is involved in causing gastric ulcers and stomach cancers. Its two nickel dependent enzymes - Urease and NiFe-H2ase, provides it a unique ability to survive and colonize in acidic human stomach. Urease catalyzes the conversion of urea to ammonia and carbamate which neutralizes its local acidic environment. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>UreE is an essential metallochaeprone for ''Helicobacter pylori'' (''H.pylori''). ''H.pylori'' is a class I human pathogen that is involved in causing gastric ulcers and stomach cancers. Its two nickel dependent enzymes - Urease and NiFe-H2ase, provides it a unique ability to survive and colonize in acidic human stomach. Urease catalyzes the conversion of urea to ammonia and carbamate which neutralizes its local acidic environment. </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Function ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Function ==</div></td></tr> </table>

Priyanka Basak http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071857&oldid=prev Priyanka Basak at 20:30, 2 December 2014 2014-12-02T20:30:56Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:30, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.<del style="color: red; font-weight: bold; text-decoration: none;">[[Image:HpUreE.png size='300' side='right']]</del>''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> </table>

Priyanka Basak http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071856&oldid=prev Priyanka Basak at 20:30, 2 December 2014 2014-12-02T20:30:32Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:30, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.<ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:HpUreE.png size='300' side='right']]</ins>''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> </table>

Priyanka Basak http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071855&oldid=prev Priyanka Basak at 20:27, 2 December 2014 2014-12-02T20:27:12Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:27, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.<del style="color: red; font-weight: bold; text-decoration: none;">[[Image:HpUreE.png]] </del>''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> </table>

Priyanka Basak http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071854&oldid=prev Priyanka Basak at 20:26, 2 December 2014 2014-12-02T20:26:04Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:26, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png<del style="color: red; font-weight: bold; text-decoration: none;">/300px/left</del>]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> </table>

Priyanka Basak http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071853&oldid=prev Priyanka Basak at 20:25, 2 December 2014 2014-12-02T20:25:45Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:25, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png<del style="color: red; font-weight: bold; text-decoration: none;">/</del>/300px/<del style="color: red; font-weight: bold; text-decoration: none;">right/</del>]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png/300px/<ins style="color: red; font-weight: bold; text-decoration: none;">left</ins>]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> </table>

Priyanka Basak http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071852&oldid=prev Priyanka Basak at 20:23, 2 December 2014 2014-12-02T20:23:25Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:23, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.[[Image:HpUreE.png<ins style="color: red; font-weight: bold; text-decoration: none;">//300px/right/</ins>]] ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> </table>

Priyanka Basak http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071850&oldid=prev Priyanka Basak at 20:17, 2 December 2014 2014-12-02T20:17:12Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:17, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer. ''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer.<ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:HpUreE.png]] </ins>''Hp''UreE can bind one Ni(II) per dimer <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer). </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> </table>

Priyanka Basak http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071847&oldid=prev Priyanka Basak at 20:07, 2 December 2014 2014-12-02T20:07:03Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:07, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific nickel metallochaperone that is involved in the delivery nickel to urease.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer <del style="color: red; font-weight: bold; text-decoration: none;">which becomes tetrameric (dimer of dimer) when bound to metal</del>. ''Hp''UreE can bind one Ni(II) <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer. ''Hp''UreE can bind one Ni(II) <ins style="color: red; font-weight: bold; text-decoration: none;">per dimer </ins><scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>(UreE-Nickel Binding site)</scene><ins style="color: red; font-weight: bold; text-decoration: none;">.In presence of Ni, its oligomeric state changes to a tetramer (dimer of dimer)</ins>. </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> </table>

Priyanka Basak http://52.214.119.220/wiki/index.php?title=Priyanka_Basak/Sandbox&diff=2071846&oldid=prev Priyanka Basak at 20:02, 2 December 2014 2014-12-02T20:02:42Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:02, 2 December 2014</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 3:</td> <td colspan="2" class="diff-lineno">Line 3:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>UreE is an essential metallochaeprone for ''Helicobacter pylori'' (''H.pylori''). ''H.pylori'' is a class I human pathogen that is involved in causing gastric ulcers and stomach cancers. Its two nickel dependent enzymes - Urease and NiFe-H2ase, provides it a unique ability to survive and colonize in acidic human stomach. Urease catalyzes the conversion of urea to ammonia and carbamate which neutralizes its local acidic environment. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>UreE is an essential metallochaeprone for ''Helicobacter pylori'' (''H.pylori''). ''H.pylori'' is a class I human pathogen that is involved in causing gastric ulcers and stomach cancers. Its two nickel dependent enzymes - Urease and NiFe-H2ase, provides it a unique ability to survive and colonize in acidic human stomach. Urease catalyzes the conversion of urea to ammonia and carbamate which neutralizes its local acidic environment. </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Function ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Function ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific <del style="color: red; font-weight: bold; text-decoration: none;">Nickel </del>metallochaperone that is involved in the delivery nickel to urease.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Several accessory proteins play a crucial role in the maturation of Urease. UreE is a urease-specific <ins style="color: red; font-weight: bold; text-decoration: none;">nickel </ins>metallochaperone that is involved in the delivery nickel to urease.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Structural highlights ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer which becomes tetrameric (dimer of dimer) when bound to metal. ''Hp''UreE can bind one Ni(II) <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound <del style="color: red; font-weight: bold; text-decoration: none;">to </del>UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'>UreE-Nickel Binding site</scene>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Apo-UreE from ''H.pylori'' is a dimer which becomes tetrameric (dimer of dimer) when bound to metal. ''Hp''UreE can bind one Ni(II) <scene name='60/609775/Nickel_bound_uree/5'>Nickel bound UreE</scene>. Nickel is bound to UreE in an octahedral fashion with one His from one subunit and two His from other subunit <scene name='60/609775/Nickel_bound_uree_2/2'><ins style="color: red; font-weight: bold; text-decoration: none;">(</ins>UreE-Nickel Binding site<ins style="color: red; font-weight: bold; text-decoration: none;">)</ins></scene>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> </table>

Priyanka Basak