Streptavidin Binding Site - Revision history

Michal Harel at 09:23, 1 January 2015

2015-01-01T09:23:13Z

←Older revision		Revision as of 09:23, 1 January 2015
Line 1:		Line 1:
	==Streptavidin binds biotin with extraordinary affinity==		==Streptavidin binds biotin with extraordinary affinity==
	<StructureSection load='' size='340' side='right' caption='Biotin, bound to streptavidin' scene='58/580641/Biotin_bs/1'>		<StructureSection load='' size='340' side='right' caption='Biotin, bound to streptavidin' scene='58/580641/Biotin_bs/1'>
-	Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the 3D structure of <scene name='58/580641/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).	+	'''Streptavidin''', from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the 3D structure of <scene name='58/580641/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).
	{\|		{\|
	\|[[Image:Biotin structure.png]]		\|[[Image:Biotin structure.png]]

Angel Herraez at 10:03, 19 March 2014

2014-03-19T10:03:46Z

←Older revision		Revision as of 10:03, 19 March 2014
Line 1:		Line 1:
	==Streptavidin binds biotin with extraordinary affinity==		==Streptavidin binds biotin with extraordinary affinity==
	<StructureSection load='' size='340' side='right' caption='Biotin, bound to streptavidin' scene='58/580641/Biotin_bs/1'>		<StructureSection load='' size='340' side='right' caption='Biotin, bound to streptavidin' scene='58/580641/Biotin_bs/1'>
-	Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the structure of <scene name='58/580641/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).	+	Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the 3D structure of <scene name='58/580641/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).
	{\|		{\|
	\|[[Image:Biotin structure.png]]		\|[[Image:Biotin structure.png]]
		+	\|align="center"\| Biotin
	\|[[Image:Biotin 3D balls.png]]		\|[[Image:Biotin 3D balls.png]]
-	\|-
-	\|align="center"\| Biotin
-	\|align="center"\| Biotin
	\|}		\|}

Angel Herraez at 09:43, 19 March 2014

2014-03-19T09:43:02Z

←Older revision		Revision as of 09:43, 19 March 2014
Line 1:		Line 1:
	==Streptavidin binds biotin with extraordinary affinity==		==Streptavidin binds biotin with extraordinary affinity==
-	<StructureSection load='' size='340' side='right' caption='Biotin, bound to streptavidin' scene='54/~~547133~~/Biotin_bs/1'>	+	<StructureSection load='' size='340' side='right' caption='Biotin, bound to streptavidin' scene='58/580641/Biotin_bs/1'>
	Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the structure of <scene name='58/580641/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).		Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the structure of <scene name='58/580641/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).
	{\|		{\|

Angel Herraez: preparing the page (scenes are still missing)

2014-03-19T09:24:19Z

preparing the page (scenes are still missing)

←Older revision		Revision as of 09:24, 19 March 2014
Line 1:		Line 1:
	==Streptavidin binds biotin with extraordinary affinity==		==Streptavidin binds biotin with extraordinary affinity==
-	<StructureSection load='' size='340' side='right' caption='Biotin' scene='54/547133/Biotin_bs/1'>	+	<StructureSection load='' size='340' side='right' caption='Biotin, bound to streptavidin' scene='54/547133/Biotin_bs/1'>
-		+
	Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the structure of <scene name='58/580641/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).		Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the structure of <scene name='58/580641/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).
-	You can read more details at [[Streptavidin\|~~Proteopedia's~~ avidin and streptavidin page]]	+	{\|
		+	\|[[Image:Biotin structure.png]]
		+	\|[[Image:Biotin 3D balls.png]]
		+	\|-
		+	\|align="center"\| Biotin
		+	\|align="center"\| Biotin
		+	\|}
		+
		+	You can read more details at the [[Streptavidin\|avidin and streptavidin page]]

	This page focuses on the binding site for biotin and how two of the subunits collaborate in binding.		This page focuses on the binding site for biotin and how two of the subunits collaborate in binding.

Student at 09:10, 19 March 2014

2014-03-19T09:10:21Z

←Older revision		Revision as of 09:10, 19 March 2014
Line 2:		Line 2:
	<StructureSection load='' size='340' side='right' caption='Biotin' scene='54/547133/Biotin_bs/1'>		<StructureSection load='' size='340' side='right' caption='Biotin' scene='54/547133/Biotin_bs/1'>

-	Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the structure of <scene name='54/~~547133~~/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).	+	Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the structure of <scene name='58/580641/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).
	You can read more details at [[Streptavidin\|Proteopedia's avidin and streptavidin page]]		You can read more details at [[Streptavidin\|Proteopedia's avidin and streptavidin page]]

	This page focuses on the binding site for biotin and how two of the subunits collaborate in binding.		This page focuses on the binding site for biotin and how two of the subunits collaborate in binding.

-	The <scene name='54/~~547133~~/One_subunit/1'>first display</scene> shows a single subunit of streptavidin with its bound biotin.	+	The <scene name='58/580641/One_subunit/1'>first display</scene> shows a single subunit of streptavidin with its bound biotin.

-	What does the binding site look like? Let's display the <scene name='54/~~547133~~/One_subunit_solid/1'>solid volume of the protein</scene> (in pale blue).	+	What does the binding site look like? Let's display the <scene name='58/580641/One_subunit_solid/1'>solid volume of the protein</scene> (in pale blue).
	As you see, biotin is embedded inside streptavidin, but a considerable –and hydrophobic– part of the biotin molecule remains exposed.		As you see, biotin is embedded inside streptavidin, but a considerable –and hydrophobic– part of the biotin molecule remains exposed.

-	However, when we add the <scene name='54/~~547133~~/One_subunit_solid_solidcover/1'>portion of the neighbouring streptavidin subunit</scene> (residues 116 to 121) that contacts with the first subunit, you will see that it covers most of the biotin molecule.	+	However, when we add the <scene name='58/580641/One_subunit_solid_solidcover/1'>portion of the neighbouring streptavidin subunit</scene> (residues 116 to 121) that contacts with the first subunit, you will see that it covers most of the biotin molecule.

	Now only the carboxylate group of biotin (note the two red oxygens) sticks out.		Now only the carboxylate group of biotin (note the two red oxygens) sticks out.

Angel Herraez: new page with a simple, quick to load, model of a single subunit

2014-03-19T09:02:32Z

new page with a simple, quick to load, model of a single subunit

New page

==Streptavidin binds biotin with extraordinary affinity==
<StructureSection load='' size='340' side='right' caption='Biotin' scene='54/547133/Biotin_bs/1'>

Streptavidin, from the bacteria ''Streptomyces avidinii'', is a tetrameric protein that has a strong affinity for binding '''biotin''' (the structure of <scene name='54/547133/Biotin_bs/1'>biotin</scene> is now displayed at the right-hand panel).
You can read more details at [[Streptavidin|Proteopedia's avidin and streptavidin page]]

This page focuses on the binding site for biotin and how two of the subunits collaborate in binding.

The <scene name='54/547133/One_subunit/1'>first display</scene> shows a single subunit of streptavidin with its bound biotin.

What does the binding site look like? Let's display the <scene name='54/547133/One_subunit_solid/1'>solid volume of the protein</scene> (in pale blue).
As you see, biotin is embedded inside streptavidin, but a considerable –and hydrophobic– part of the biotin molecule remains exposed.

However, when we add the <scene name='54/547133/One_subunit_solid_solidcover/1'>portion of the neighbouring streptavidin subunit</scene> (residues 116 to 121) that contacts with the first subunit, you will see that it covers most of the biotin molecule.

Now only the carboxylate group of biotin (note the two red oxygens) sticks out.

</StructureSection>