Thiolase - Revision history

Michal Harel at 09:18, 20 December 2023

2023-12-20T09:18:40Z

←Older revision		Revision as of 09:18, 20 December 2023
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	For bacterial thiolase see [[Bacterial thiolase]]		For bacterial thiolase see [[Bacterial thiolase]]

-	For zebrafish thiolase see [[SCP2-thiolase]]	+	For zebrafish thiolase see [[SCP2-thiolase]]. ''' SCP''' is Sterol Carrier Protein.

	See also [[Mevalonate pathway]]		See also [[Mevalonate pathway]]

Alexander Berchansky at 11:34, 16 February 2023

2023-02-16T11:34:09Z

←Older revision		Revision as of 11:34, 16 February 2023
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	== Function ==		== Function ==
	Two different classes of '''thiolases''' are found.<br />		Two different classes of '''thiolases''' are found.<br />
-	*'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid β oxidation<ref>PMID:16630629</ref>.<br />	+	*'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid [[beta oxidation]]<ref>PMID:16630629</ref>.<br />
	*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) or '''acetyl-CoA acetyltransferase''' are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.		*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) or '''acetyl-CoA acetyltransferase''' are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.

Michal Harel at 08:33, 16 January 2023

2023-01-16T08:33:08Z

←Older revision		Revision as of 08:33, 16 January 2023
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	For zebrafish thiolase see [[SCP2-thiolase]]		For zebrafish thiolase see [[SCP2-thiolase]]
		+
		+	See also [[Mevalonate pathway]]

	== Structural highlights ==		== Structural highlights ==

Alexander Berchansky at 12:13, 29 September 2022

2022-09-29T12:13:43Z

←Older revision		Revision as of 12:13, 29 September 2022
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-	<StructureSection load='4c2j' size='350' side='right' caption='Human 3-ketoacyl-CoA complex with CoA and ethylene glycol(PDB code [[4c2j]]).' scene='44/447919/Cv/7'>	+	<StructureSection load='4c2j' size='350' side='right' caption='Human 3-ketoacyl-CoA complex with CoA and ethylene glycol (PDB code [[4c2j]])' scene='44/447919/Cv/7'>
	== Function ==		== Function ==
	Two different classes of '''thiolases''' are found.<br />		Two different classes of '''thiolases''' are found.<br />

Michal Harel at 09:20, 26 November 2020

2020-11-26T09:20:47Z

←Older revision		Revision as of 09:20, 26 November 2020
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	Two different classes of '''thiolases''' are found.<br />		Two different classes of '''thiolases''' are found.<br />
	*'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid β oxidation<ref>PMID:16630629</ref>.<br />		*'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid β oxidation<ref>PMID:16630629</ref>.<br />
-	*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.	+	*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) or '''acetyl-CoA acetyltransferase''' are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.

	For bacterial thiolase see [[Bacterial thiolase]]		For bacterial thiolase see [[Bacterial thiolase]]

Michal Harel at 11:02, 20 February 2020

2020-02-20T11:02:21Z

←Older revision		Revision as of 11:02, 20 February 2020
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	</StructureSection>		</StructureSection>
-	== 3D Structures of Thiolase ==

-	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-	{{#tree:id=OrganizedByTopic\|openlevels=0\|
-
-	*3-ketoacyl-CoA thiolase
-
-	**[[2iik]] – hKCT peroxisomal – human<br />
-	**[[4c2k]] – hKCT mitochondrial <br />
-	**[[4c2j]] – hKCT mitochondrial + CoA<br />
-	**[[2wu9]], [[2c7y]], [[2c7z]] – KCT peroxisomal residues 36-462 – ''Arabidopsis thaliana''<br />
-	**[[2wua]] - KCT peroxisomal residues 17-449 (mutant) – sunflower<br />
-	**[[1afw]], [[1pxt]] – yKCT peroxisomal – yeast<br />
-	**[[3goa]] – KCT – ''Salmonella typhimurium''<br />
-	**[[5lp7]] – KCT – ''Bacillus subtilis'' <br />
-	**[[5cbq]] – MsKCT – ''Mycobacterium smegmatis'' <br />
-	**[[4w61]] – ReKCT – ''Ralstonia eutropha''<br />
-	**[[4ubw]], [[5onc]] – MtKCT – ''Mycobacterium tuberculosis'' <br />
-
-	*3-ketoacyl-CoA thiolase complex
-
-	**[[3w15]] - yKCT peroxisomal residues 27-396 + peroxiomal membrane protein PEX21 + peroxisomal targeting signal receptor<br />
-	**[[2d3t]] – PfKCT + fatty oxidation complex β subunit – ''Pseudomonas fragi''<br />
-	**[[1wdk]], [[1wdl]], [[1wdm]] - PfKCT + fatty oxidation complex α subunit<br />
-	**[[5bz4]] – MsKCT + CoA <br />
-	**[[4ubt]] – MtKCT (mutant) + CoA + steroid <br />
-	**[[4ubu]] – MtKCT (mutant) + CoA <br />
-	**[[4ubv]] – MtKCT + CoA + acetyl CoA <br />
-
-	*Acetoacetyl-CoA thiolase or acetyl-CoA acetyltransferase
-
-	**[[2ib7]], [[2ib8]], [[2ib9]], [[2ibu]], [[2ibw]], [[2iby]], [[2f2s]] – hACT mitochondrial<br />
-	**[[1wl5]] - hACT cytosolic<br />
-	**[[5xyj]], [[5xz5]] – yACT <br />
-	**[[1m4s]], [[1m4t]], [[1dlu]], [[1qfl]] - ZrACT – ''Zoogloea ramigera''<br />
-	**[[2wku]], [[2wl6]], [[1m1t]], [[1m3k]] - ZrACT (mutant) <br />
-	**[[4dd5]], [[4e1l]] – ACT – ''Clostridium difficile''<br />
-	**[[4xl2]], [[4xl3]], [[4n44]], [[4n45]] – CaACT – ''Clostridium acetobutylicum''<br />
-	**[[4wyr]] – CaACT (mutant) <br />
-	**[[5f0v]], [[5f38]], [[4wys]] – ACT – ''Escherichia coli'' <br />
-	**[[4o9a]], [[4o99]], [[4o9c]], [[4nzs]] – ReACT <br />
-
-	*Acetoacetyl-CoA thiolase complex
-
-	**[[1wl4]] – hACT cytosolic + CoA <br />
-	**[[1dm3]], [[1dlv]] – ZrACT + CoA<br />
-	**[[2wkt]], [[2wkv]], [[2wl4]], [[2wl5]], [[2vtz]], [[1m3z]] – ZrACT (mutant) + CoA<br />
-	**[[1m1o]] - ZrACT (mutant) + acetoacetyl-CoA<br />
-	**[[2vu0]], [[1nl7]] - ZrACT + CoA<br />
-	**[[2vu1]], [[2vu2]], [[1ou6]] - ZrACT + pantheteine-11-pivalate<br />
-	**[[4xl4]] – CaACT + CoA <br />
-	**[[6et9]] – MtACT + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase - ''Methanothermococcus thermolithotrophicus''<br />
-	**[[6esq]] – MtACT + CoA + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase <br />
-	**[[6are]] – ACT + CoA + acetate - ''Aspergillus fumigatus'' <br />
-
-	*SCP2-thiolase
-
-	**]]6hrv]] – zfSCP2 - zebrafish <br />
-	**]]6hsj]] – zfSCP2 + CoA<br />
-	**]]6hsp]] – zfSCP2 + CoA + octanoyl-CoA<br />
-	}}
	== References ==		== References ==
	<references/>		<references/>
	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 11:01, 20 February 2020

2020-02-20T11:01:27Z

←Older revision		Revision as of 11:01, 20 February 2020
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	== Structural highlights ==		== Structural highlights ==
	The <scene name='44/447919/Cv/8'>active site of 3-ketoacyl-CoA thiolase contains CoA</scene><ref>PMID:25478839</ref>. Water molecules are shown as red spheres.		The <scene name='44/447919/Cv/8'>active site of 3-ketoacyl-CoA thiolase contains CoA</scene><ref>PMID:25478839</ref>. Water molecules are shown as red spheres.
		+	== 3D Structures of Thiolase ==
		+	[[Thiolase 3D structures]]
		+
	</StructureSection>		</StructureSection>
	== 3D Structures of Thiolase ==		== 3D Structures of Thiolase ==
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	*3-ketoacyl-CoA thiolase		*3-ketoacyl-CoA thiolase

-	**[[2wu9]], [[2c7y]], [[2c7z]] – KCT peroxisomal residues 36-462 – ''Arabidopsis thaliana''<br />
-	**[[2wua]] - KCT peroxisomal residues 17-449 (mutant) – sunflower<br />
	**[[2iik]] – hKCT peroxisomal – human<br />		**[[2iik]] – hKCT peroxisomal – human<br />
	**[[4c2k]] – hKCT mitochondrial <br />		**[[4c2k]] – hKCT mitochondrial <br />
	**[[4c2j]] – hKCT mitochondrial + CoA<br />		**[[4c2j]] – hKCT mitochondrial + CoA<br />
		+	**[[2wu9]], [[2c7y]], [[2c7z]] – KCT peroxisomal residues 36-462 – ''Arabidopsis thaliana''<br />
		+	**[[2wua]] - KCT peroxisomal residues 17-449 (mutant) – sunflower<br />
		+	**[[1afw]], [[1pxt]] – yKCT peroxisomal – yeast<br />
	**[[3goa]] – KCT – ''Salmonella typhimurium''<br />		**[[3goa]] – KCT – ''Salmonella typhimurium''<br />
	**[[5lp7]] – KCT – ''Bacillus subtilis'' <br />		**[[5lp7]] – KCT – ''Bacillus subtilis'' <br />
-	**[[1afw]], [[1pxt]] – yKCT peroxisomal – yeast<br />
	**[[5cbq]] – MsKCT – ''Mycobacterium smegmatis'' <br />		**[[5cbq]] – MsKCT – ''Mycobacterium smegmatis'' <br />
	**[[4w61]] – ReKCT – ''Ralstonia eutropha''<br />		**[[4w61]] – ReKCT – ''Ralstonia eutropha''<br />
Line 43:		Line 46:
	*Acetoacetyl-CoA thiolase or acetyl-CoA acetyltransferase		*Acetoacetyl-CoA thiolase or acetyl-CoA acetyltransferase

-	**[[1m4s]], [[1m4t]], [[1dlu]], [[1qfl]] - ZrACT – ''Zoogloea ramigera''<br />
-	**[[2wku]], [[2wl6]], [[1m1t]], [[1m3k]] - ZrACT (mutant) <br />
	**[[2ib7]], [[2ib8]], [[2ib9]], [[2ibu]], [[2ibw]], [[2iby]], [[2f2s]] – hACT mitochondrial<br />		**[[2ib7]], [[2ib8]], [[2ib9]], [[2ibu]], [[2ibw]], [[2iby]], [[2f2s]] – hACT mitochondrial<br />
	**[[1wl5]] - hACT cytosolic<br />		**[[1wl5]] - hACT cytosolic<br />
		+	**[[5xyj]], [[5xz5]] – yACT <br />
		+	**[[1m4s]], [[1m4t]], [[1dlu]], [[1qfl]] - ZrACT – ''Zoogloea ramigera''<br />
		+	**[[2wku]], [[2wl6]], [[1m1t]], [[1m3k]] - ZrACT (mutant) <br />
	**[[4dd5]], [[4e1l]] – ACT – ''Clostridium difficile''<br />		**[[4dd5]], [[4e1l]] – ACT – ''Clostridium difficile''<br />
	**[[4xl2]], [[4xl3]], [[4n44]], [[4n45]] – CaACT – ''Clostridium acetobutylicum''<br />		**[[4xl2]], [[4xl3]], [[4n44]], [[4n45]] – CaACT – ''Clostridium acetobutylicum''<br />
Line 52:		Line 56:
	**[[5f0v]], [[5f38]], [[4wys]] – ACT – ''Escherichia coli'' <br />		**[[5f0v]], [[5f38]], [[4wys]] – ACT – ''Escherichia coli'' <br />
	**[[4o9a]], [[4o99]], [[4o9c]], [[4nzs]] – ReACT <br />		**[[4o9a]], [[4o99]], [[4o9c]], [[4nzs]] – ReACT <br />
-	**[[5xyj]], [[5xz5]] – yACT <br />

	*Acetoacetyl-CoA thiolase complex		*Acetoacetyl-CoA thiolase complex

		+	**[[1wl4]] – hACT cytosolic + CoA <br />
	**[[1dm3]], [[1dlv]] – ZrACT + CoA<br />		**[[1dm3]], [[1dlv]] – ZrACT + CoA<br />
	**[[2wkt]], [[2wkv]], [[2wl4]], [[2wl5]], [[2vtz]], [[1m3z]] – ZrACT (mutant) + CoA<br />		**[[2wkt]], [[2wkv]], [[2wl4]], [[2wl5]], [[2vtz]], [[1m3z]] – ZrACT (mutant) + CoA<br />
Line 61:		Line 65:
	**[[2vu0]], [[1nl7]] - ZrACT + CoA<br />		**[[2vu0]], [[1nl7]] - ZrACT + CoA<br />
	**[[2vu1]], [[2vu2]], [[1ou6]] - ZrACT + pantheteine-11-pivalate<br />		**[[2vu1]], [[2vu2]], [[1ou6]] - ZrACT + pantheteine-11-pivalate<br />
-	**[[1wl4]] – hACT cytosolic + CoA <br />
	**[[4xl4]] – CaACT + CoA <br />		**[[4xl4]] – CaACT + CoA <br />
	**[[6et9]] – MtACT + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase - ''Methanothermococcus thermolithotrophicus''<br />		**[[6et9]] – MtACT + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase - ''Methanothermococcus thermolithotrophicus''<br />
	**[[6esq]] – MtACT + CoA + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase <br />		**[[6esq]] – MtACT + CoA + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase <br />
	**[[6are]] – ACT + CoA + acetate - ''Aspergillus fumigatus'' <br />		**[[6are]] – ACT + CoA + acetate - ''Aspergillus fumigatus'' <br />
		+
		+	*SCP2-thiolase
		+
		+	**]]6hrv]] – zfSCP2 - zebrafish <br />
		+	**]]6hsj]] – zfSCP2 + CoA<br />
		+	**]]6hsp]] – zfSCP2 + CoA + octanoyl-CoA<br />
	}}		}}
	== References ==		== References ==
	<references/>		<references/>
	[[Category:Topic Page]]		[[Category:Topic Page]]

Michal Harel at 08:45, 19 January 2020

2020-01-19T08:45:37Z

←Older revision		Revision as of 08:45, 19 January 2020
Line 5:		Line 5:
	*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.		*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.

-	For ~~Bacterial~~ thiolase see [[Bacterial thiolase]]	+	For bacterial thiolase see [[Bacterial thiolase]]
		+
		+	For zebrafish thiolase see [[SCP2-thiolase]]

	== Structural highlights ==		== Structural highlights ==

Alexander Berchansky at 13:26, 22 September 2019

2019-09-22T13:26:52Z

←Older revision		Revision as of 13:26, 22 September 2019
Line 8:		Line 8:

	== Structural highlights ==		== Structural highlights ==
-	The <scene name='44/447919/Cv/2'>active site of 3-ketoacyl-CoA thiolase contains CoA</scene><ref>PMID:25478839</ref>. Water molecules shown as red spheres.	+	The <scene name='44/447919/Cv/8'>active site of 3-ketoacyl-CoA thiolase contains CoA</scene><ref>PMID:25478839</ref>. Water molecules are shown as red spheres.
	</StructureSection>		</StructureSection>
	== 3D Structures of Thiolase ==		== 3D Structures of Thiolase ==

Michal Harel at 19:17, 25 September 2018

2018-09-25T19:17:11Z

←Older revision		Revision as of 19:17, 25 September 2018
Line 1:		Line 1:
-	<StructureSection load='4c2j' size='~~450~~' side='right' caption='Human 3-ketoacyl-CoA complex with CoA and ethylene glycol(PDB code [[4c2j]]).' scene='44/447919/Cv/7'>	+	<StructureSection load='4c2j' size='350' side='right' caption='Human 3-ketoacyl-CoA complex with CoA and ethylene glycol(PDB code [[4c2j]]).' scene='44/447919/Cv/7'>
	== Function ==		== Function ==
	Two different classes of '''thiolases''' are found.<br />		Two different classes of '''thiolases''' are found.<br />
Line 39:		Line 39:
	**[[4ubv]] – MtKCT + CoA + acetyl CoA <br />		**[[4ubv]] – MtKCT + CoA + acetyl CoA <br />

-	*Acetoacetyl-CoA thiolase	+	*Acetoacetyl-CoA thiolase or acetyl-CoA acetyltransferase

	**[[1m4s]], [[1m4t]], [[1dlu]], [[1qfl]] - ZrACT – ''Zoogloea ramigera''<br />		**[[1m4s]], [[1m4t]], [[1dlu]], [[1qfl]] - ZrACT – ''Zoogloea ramigera''<br />
Line 50:		Line 50:
	**[[5f0v]], [[5f38]], [[4wys]] – ACT – ''Escherichia coli'' <br />		**[[5f0v]], [[5f38]], [[4wys]] – ACT – ''Escherichia coli'' <br />
	**[[4o9a]], [[4o99]], [[4o9c]], [[4nzs]] – ReACT <br />		**[[4o9a]], [[4o99]], [[4o9c]], [[4nzs]] – ReACT <br />
		+	**[[5xyj]], [[5xz5]] – yACT <br />

	*Acetoacetyl-CoA thiolase complex		*Acetoacetyl-CoA thiolase complex
Line 60:		Line 61:
	**[[1wl4]] – hACT cytosolic + CoA <br />		**[[1wl4]] – hACT cytosolic + CoA <br />
	**[[4xl4]] – CaACT + CoA <br />		**[[4xl4]] – CaACT + CoA <br />
-		+	**[[6et9]] – MtACT + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase - ''Methanothermococcus thermolithotrophicus''<br />
		+	**[[6esq]] – MtACT + CoA + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase <br />
		+	**[[6are]] – ACT + CoA + acetate - ''Aspergillus fumigatus'' <br />
	}}		}}
	== References ==		== References ==
	<references/>		<references/>
	[[Category:Topic Page]]		[[Category:Topic Page]]