User:Wayne Decatur/Sandbox Haloarcula Ribosomal Large Subunit - Revision history

Wayne Decatur: Redirecting to User:Wayne Decatur/Haloarcula Large Ribosomal Subunit

2010-01-10T00:43:09Z

Redirecting to User:Wayne Decatur/Haloarcula Large Ribosomal Subunit

Wayne Decatur at 08:02, 31 December 2009

2009-12-31T08:02:19Z

←Older revision		Revision as of 08:02, 31 December 2009
Line 185:		Line 185:
	[[Category: Antibiotic]]		[[Category: Antibiotic]]
	[[Category: Puromycin]]		[[Category: Puromycin]]
-	[[[[Category: Macrolide]]	+	[[Category: Macrolide]]
	[[Category: Azithromycin]]		[[Category: Azithromycin]]
	[[Category: Zithromax]]		[[Category: Zithromax]]

Wayne Decatur at 08:01, 31 December 2009

2009-12-31T08:01:42Z

←Older revision		Revision as of 08:01, 31 December 2009
Line 184:		Line 184:
	[[Category: Intrinsically Unfolded Proteins]]		[[Category: Intrinsically Unfolded Proteins]]
	[[Category: Antibiotic]]		[[Category: Antibiotic]]
-	[[Category: ~~puromycin~~]]	+	[[Category: Puromycin]]
		+	[[[[Category: Macrolide]]
		+	[[Category: Azithromycin]]
		+	[[Category: Zithromax]]
	[[Category: tRNA]]		[[Category: tRNA]]
	[[Category: Rna-rna]]		[[Category: Rna-rna]]

Wayne Decatur at 07:47, 31 December 2009

2009-12-31T07:47:59Z

←Older revision		Revision as of 07:47, 31 December 2009
Line 150:		Line 150:
	:*Each amino acid added to the growing peptide chain will begin its journey through <font color="dodgerblue">'''the tunnel'''</font> at this end.		:*Each amino acid added to the growing peptide chain will begin its journey through <font color="dodgerblue">'''the tunnel'''</font> at this end.
	:*The <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72tunnelstartyarus/4'>start of the tunnel corresponds to the site where the Yarus analog (magenta) binds</scene> in the active site. This makes sense since the analog represents the intermediate in peptide bond synthesis.		:*The <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72tunnelstartyarus/4'>start of the tunnel corresponds to the site where the Yarus analog (magenta) binds</scene> in the active site. This makes sense since the analog represents the intermediate in peptide bond synthesis.
		+	:*A number of antibiotics, including the [[User:Wayne Decatur/Haloarcula Large Ribosomal Subunit With Azithromycin\|macrolide Azithromycin (zithromax)]], binds to this region of the tunnel.

Wayne Decatur at 07:24, 31 December 2009

2009-12-31T07:24:39Z

←Older revision		Revision as of 07:24, 31 December 2009
Line 171:		Line 171:
	*[http://www.rcsb.org/pdb/static.do?p=general_information/news_publications/news/news_2009.html#20091013 RCSB Protein Data Bank coverage of the 2009 Nobel Prizes in Chemistry]		*[http://www.rcsb.org/pdb/static.do?p=general_information/news_publications/news/news_2009.html#20091013 RCSB Protein Data Bank coverage of the 2009 Nobel Prizes in Chemistry]
	*[http://www.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb10_1.html Ribosome: October 2000 Molecule of the Month] as part of the series of tutorials that are at [http://www.pdb.org/pdb/home/home.do the RCSB Protein Data Bank] and written by [[User:David_S._Goodsell\|David Goodsell]]		*[http://www.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb10_1.html Ribosome: October 2000 Molecule of the Month] as part of the series of tutorials that are at [http://www.pdb.org/pdb/home/home.do the RCSB Protein Data Bank] and written by [[User:David_S._Goodsell\|David Goodsell]]
		+	[[Category: Haloarcula marismortui]]
		+	[[Category: Klein, D J.]]
		+	[[Category: Moore, P B.]]
		+	[[Category: Schmeing, T M.]]
		+	[[Category: Steitz, T A.]]
		+	[[Category: Protein-protein]]
		+	[[Category: Protein-rna]]
		+	[[Category: Ribosome assembly]]
		+	[[Category: tunnel]]
		+	[[Category: ribozyme]]
		+	[[Category: Intrinsically Unfolded Proteins]]
		+	[[Category: Antibiotic]]
		+	[[Category: puromycin]]
		+	[[Category: tRNA]]
		+	[[Category: Rna-rna]]
		+	[[Category: 50S]]
		+	[[Category: Large ribosomal subunit]]
		+	[[Category: Ribosome]]

Wayne Decatur at 04:13, 31 December 2009

2009-12-31T04:13:03Z

←Older revision		Revision as of 04:13, 31 December 2009
Line 127:		Line 127:
	:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.		:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.
	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/5'>Guanosine 2284 (orange) and Guanosine 2285 (blue) are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.		::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/5'>Guanosine 2284 (orange) and Guanosine 2285 (blue) are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.
-	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s722486yarus/1'>One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction</scene><ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>.	+	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s722486yarus/1'>One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction</scene><ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>. The individual components of the <font color="magenta">'''Yarus analog'''</font> are labeled.
	::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.		::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.
	:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72ribozyme/13'>No proteins approach close enough to the active site to affect the chemistry of peptide bond synthesis</scene>		:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72ribozyme/13'>No proteins approach close enough to the active site to affect the chemistry of peptide bond synthesis</scene>

Wayne Decatur at 04:08, 31 December 2009

2009-12-31T04:08:42Z

←Older revision		Revision as of 04:08, 31 December 2009
Line 127:		Line 127:
	:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.		:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.
	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/5'>Guanosine 2284 (orange) and Guanosine 2285 (blue) are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.		::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/5'>Guanosine 2284 (orange) and Guanosine 2285 (blue) are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.
-	::*One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction<ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>.	+	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s722486yarus/1'>One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction</scene><ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>.
	::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.		::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.
	:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72ribozyme/13'>No proteins approach close enough to the active site to affect the chemistry of peptide bond synthesis</scene>		:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72ribozyme/13'>No proteins approach close enough to the active site to affect the chemistry of peptide bond synthesis</scene>

Wayne Decatur at 03:50, 31 December 2009

2009-12-31T03:50:55Z

←Older revision		Revision as of 03:50, 31 December 2009
Line 126:		Line 126:
	:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5allwithyarus/9'>An informative analog is observed at the core of the large subunit bound to Domain V</scene> (<font color = "red">'''shown in red'''</font>)		:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5allwithyarus/9'>An informative analog is observed at the core of the large subunit bound to Domain V</scene> (<font color = "red">'''shown in red'''</font>)
	:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.		:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.
-	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/4'>Guanosine 2284 (orange) and Guanosine 2285 (blue) are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.	+	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/5'>Guanosine 2284 (orange) and Guanosine 2285 (blue) are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.
	::*One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction<ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>.		::*One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction<ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>.
	::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.		::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.

Wayne Decatur at 03:48, 31 December 2009

2009-12-31T03:48:27Z

←Older revision		Revision as of 03:48, 31 December 2009
Line 126:		Line 126:
	:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5allwithyarus/9'>An informative analog is observed at the core of the large subunit bound to Domain V</scene> (<font color = "red">'''shown in red'''</font>)		:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5allwithyarus/9'>An informative analog is observed at the core of the large subunit bound to Domain V</scene> (<font color = "red">'''shown in red'''</font>)
	:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.		:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.
-	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/4'>Guanosine 2284 and Guanosine 2285 are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.	+	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/4'>Guanosine 2284 (orange) and Guanosine 2285 (blue) are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.
	::*One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction<ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>.		::*One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction<ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>.
	::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.		::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.

Wayne Decatur at 03:46, 31 December 2009

2009-12-31T03:46:27Z

←Older revision		Revision as of 03:46, 31 December 2009
Line 126:		Line 126:
	:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5allwithyarus/9'>An informative analog is observed at the core of the large subunit bound to Domain V</scene> (<font color = "red">'''shown in red'''</font>)		:<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5allwithyarus/9'>An informative analog is observed at the core of the large subunit bound to Domain V</scene> (<font color = "red">'''shown in red'''</font>)
	:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.		:*In addition to unliganded subunit, the large subunit structure has been solved with substrate analogs which provides a detailed view of the direct role <font color="red">'''domain V'''</font> plays in the chemistry of peptide bond synthesis. One of the analogs was the <font color="magenta">'''Yarus analog (CCA-puromycin)'''</font>, known to inhibit translation because it mimics normal substrate, specifically it resembles an unstable transition state intermediate formed during peptide bond synthesis and involving the extreme ends of the A- and P- tRNAs and atoms corresponding to parts of an amino acid. <scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarus/4'>The Yarus analog (magenta), indicating the site of the petidyl transferase reaction, is entrenched in Domain V</scene>.
-	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/4'>~~'''~~Guanosine 2284~~'''~~ and ~~'''~~Guanosine 2285~~'''~~ are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.	+	::*<scene name='User:Wayne_Decatur/Sandbox_Haloarcula_Ribosomal_Large_Subunit/1s72rrnadomain5withyarusnts/4'>Guanosine 2284 and Guanosine 2285 are base-paired</scene> with the <font color="magenta">'''Yarus analog'''</font>.
	::*One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction<ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>.		::*One atom in particular corresponds to the carbon of the tetrahedral carbon intermediate of the peptidyl transferase reaction<ref>PMID: 10937990</ref>, and <font color = "red">'''A2486'''</font> (E. coli #2451) of <font color = "red">'''Domain V'''</font> approaches this critical atom of the <font color="magenta">'''Yarus analog'''</font>.
	::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.		::*It is important to note that '''the ribosome is highly conserved, particularly the nucleotides close to the Yarus analog in Domain V and thus the major conclusions reached from the structure are applicable to all ribosomes'''.