1a2k - Revision history

OCA: Redirecting to 5bxq

OCA — Thu, 02 Mar 2023 05:55:48 GMT

Redirecting to 5bxq

←Older revision		Revision as of 05:55, 2 March 2023
Line 1:		Line 1:
-		+	#REDIRECT [[5bxq]] This PDB entry is obsolete and replaced by 5bxq
-	~~==GDPRAN-NTF2 COMPLEX==~~	+
-	~~<StructureSection load='1a2k' size='340' side='right'caption='~~[[~~1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[1a2k~~]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A2K FirstGlance]. <br>	+
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [https://pdbe.org/1a2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [https://www.ebi.ac.uk/pdbsum/1a2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a2k ProSAT]</span></td></tr>	+
-	~~</table>~~	+
-	~~== Function ==~~	+
-	~~[[https://www.uniprot.org/uniprot/NTF2_RAT NTF2_RAT]] Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62~~ and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import (By similarity). [[https://www.uniprot.org/uniprot/RAN_CANFA RAN_CANFA]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle. The complex with BIRC5/survivin plays a role in mitotic spindle formation by ~~serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (By similarity).~~	+
-	~~== Evolutionary Conservation ==~~	+
-	~~[[Image:Consurf_key_small.gif\|200px\|right]]~~	+
-	~~Check<jmol>~~	+
-	~~<jmolCheckbox>~~	+
-	~~<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a2/1a2k_consurf.spt"</scriptWhenChecked>~~	+
-	~~<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>~~	+
-	~~<text>to colour the structure by Evolutionary Conservation</text>~~	+
-	~~</jmolCheckbox>~~	+
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a2k ConSurf].	+
-	~~<div style="clear:both"></div>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Nuclear transport factor 2 (NTF2) and the Ras-family GTPase Ran are two soluble components of the nuclear protein import machinery. NTF2 binds GDP-Ran selectively and this interaction is important for efficient nuclear protein import in vivo. We have used X-ray crystallography to determine the structure of the macromolecular complex formed between GDP-Ran and nuclear transport factor 2 (NTF2) at 2.5 A resolution. The interaction interface involves primarily the putative switch II loop of Ran (residues 65 to 78) and the hydrophobic cavity and surrounding surface of NTF2. The major contribution to the interaction made by the switch II loop accounts for the ability of NTF2 to discriminate between GDP and GTP-bound forms of Ran. The aromatic side-chain of Ran Phe72 inserts into the NTF2 cavity and accounts for 22% of the surface area buried by the interaction interface, while salt bridges are formed between Lys71 and Arg76 of Ran with Asp92/Asp94 and Glu42 of NTF2, respectively. These salt bridges account for the inhibition of the Ran-NTF2 interaction by NTF2 mutants such as E42 K and D92/94N in which the negatively charged residues surrounding the cavity were altered. Because the interaction interface maintains the positions of key Ran residues involved in binding MgGDP, NTF2 binding may help stabilize the switch state of Ran, possibly in the context of targeting it to other components of the nuclear protein import machinery to specify directionality of transport. The binding of GDP-Ran at the NTF2 cavity raises the possibility that this interaction might be modulated by a metabolite or small molecule substrate for NTF2's putative enzymatic activity.	+
-		+
-	Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran.,Stewart M, Kent HM, McCoy AJ J Mol Biol. 1998 Apr 3;277(3):635-46. PMID:9533885<ref>PMID:9533885</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 1a2k" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Large Structures]]~~	+
-	~~[[Category: Kent, H M]]~~	+
-	~~[[Category: Mccoy, A J]]~~	+
-	~~[[Category: Stewart, M]]~~	+
-	~~[[Category: Gtp-binding]]~~	+
-	~~[[Category: Transport-nuclear protein complex]]~~	+

OCA at 10:15, 17 February 2021

OCA — Wed, 17 Feb 2021 10:15:47 GMT

←Older revision		Revision as of 10:15, 17 February 2021
Line 3:		Line 3:
	<StructureSection load='1a2k' size='340' side='right'caption='[[1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1a2k' size='340' side='right'caption='[[1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1A2K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A2K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [~~http~~://pdbe.org/1a2k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1a2k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1a2k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [https://pdbe.org/1a2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [https://www.ebi.ac.uk/pdbsum/1a2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a2k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NTF2_RAT NTF2_RAT]] Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import (By similarity). [[~~http~~://www.uniprot.org/uniprot/RAN_CANFA RAN_CANFA]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (By similarity).	+	[[https://www.uniprot.org/uniprot/NTF2_RAT NTF2_RAT]] Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import (By similarity). [[https://www.uniprot.org/uniprot/RAN_CANFA RAN_CANFA]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 14:31, 10 May 2019

OCA — Fri, 10 May 2019 14:31:20 GMT

←Older revision		Revision as of 14:31, 10 May 2019
Line 1:		Line 1:

	==GDPRAN-NTF2 COMPLEX==		==GDPRAN-NTF2 COMPLEX==
-	<StructureSection load='1a2k' size='340' side='right' caption='[[1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1a2k' size='340' side='right'caption='[[1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A2K FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A2K FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a2/1a2k_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a2/1a2k_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kent, H M]]		[[Category: Kent, H M]]
	[[Category: Mccoy, A J]]		[[Category: Mccoy, A J]]

OCA at 09:24, 9 August 2017

OCA — Wed, 09 Aug 2017 09:24:32 GMT

←Older revision		Revision as of 09:24, 9 August 2017
Line 1:		Line 1:
		+
	==GDPRAN-NTF2 COMPLEX==		==GDPRAN-NTF2 COMPLEX==
	<StructureSection load='1a2k' size='340' side='right' caption='[[1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1a2k' size='340' side='right' caption='[[1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Canfa Canfa]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A2K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A2K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [http://pdbe.org/1a2k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [http://www.ebi.ac.uk/pdbsum/1a2k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [http://pdbe.org/1a2k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [http://www.ebi.ac.uk/pdbsum/1a2k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1a2k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a2k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buffalo rat]]
-	[[Category: Canfa]]
	[[Category: Kent, H M]]		[[Category: Kent, H M]]
	[[Category: Mccoy, A J]]		[[Category: Mccoy, A J]]

OCA at 16:04, 9 September 2015

OCA — Wed, 09 Sep 2015 16:04:30 GMT

←Older revision		Revision as of 16:04, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1a2k' size='340' side='right' caption='[[1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1a2k' size='340' side='right' caption='[[1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Canis_lupus_familiaris Canis lupus familiaris~~] and [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A2K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Canfa Canfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A2K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [http://www.ebi.ac.uk/pdbsum/1a2k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [http://pdbe.org/1a2k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [http://www.ebi.ac.uk/pdbsum/1a2k PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1a2k" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Canis lupus familiaris~~]]	+	[[Category: Buffalo rat]]
-	[[Category: ~~Rattus norvegicus~~]]	+	[[Category: Canfa]]
	[[Category: Kent, H M]]		[[Category: Kent, H M]]
	[[Category: Mccoy, A J]]		[[Category: Mccoy, A J]]

OCA at 19:41, 25 December 2014

OCA — Thu, 25 Dec 2014 19:41:46 GMT

←Older revision		Revision as of 19:41, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [http://www.ebi.ac.uk/pdbsum/1a2k PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [http://www.ebi.ac.uk/pdbsum/1a2k PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NTF2_RAT NTF2_RAT]] Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import (By similarity). [[http://www.uniprot.org/uniprot/RAN_CANFA RAN_CANFA]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:53, 22 December 2014

OCA — Mon, 22 Dec 2014 09:53:27 GMT

←Older revision		Revision as of 09:53, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A2K FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A2K FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [http://www.ebi.ac.uk/pdbsum/1a2k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [http://www.ebi.ac.uk/pdbsum/1a2k PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	[[Category: Canis lupus familiaris]]		[[Category: Canis lupus familiaris]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
-	[[Category: Kent, H M.]]	+	[[Category: Kent, H M]]
-	[[Category: Mccoy, A J.]]	+	[[Category: Mccoy, A J]]
-	[[Category: Stewart, M.]]	+	[[Category: Stewart, M]]
	[[Category: Gtp-binding]]		[[Category: Gtp-binding]]
	[[Category: Transport-nuclear protein complex]]		[[Category: Transport-nuclear protein complex]]

OCA at 08:37, 23 July 2014

OCA — Wed, 23 Jul 2014 08:37:05 GMT

←Older revision		Revision as of 08:37, 23 July 2014
Line 1:		Line 1:
-	[[~~Image~~:1a2k.~~png\|left~~\|200px]]	+	==GDPRAN-NTF2 COMPLEX==
		+	<StructureSection load='1a2k' size='340' side='right' caption='[[1a2k]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1a2k]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A2K FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a2k RCSB], [http://www.ebi.ac.uk/pdbsum/1a2k PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a2/1a2k_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nuclear transport factor 2 (NTF2) and the Ras-family GTPase Ran are two soluble components of the nuclear protein import machinery. NTF2 binds GDP-Ran selectively and this interaction is important for efficient nuclear protein import in vivo. We have used X-ray crystallography to determine the structure of the macromolecular complex formed between GDP-Ran and nuclear transport factor 2 (NTF2) at 2.5 A resolution. The interaction interface involves primarily the putative switch II loop of Ran (residues 65 to 78) and the hydrophobic cavity and surrounding surface of NTF2. The major contribution to the interaction made by the switch II loop accounts for the ability of NTF2 to discriminate between GDP and GTP-bound forms of Ran. The aromatic side-chain of Ran Phe72 inserts into the NTF2 cavity and accounts for 22% of the surface area buried by the interaction interface, while salt bridges are formed between Lys71 and Arg76 of Ran with Asp92/Asp94 and Glu42 of NTF2, respectively. These salt bridges account for the inhibition of the Ran-NTF2 interaction by NTF2 mutants such as E42 K and D92/94N in which the negatively charged residues surrounding the cavity were altered. Because the interaction interface maintains the positions of key Ran residues involved in binding MgGDP, NTF2 binding may help stabilize the switch state of Ran, possibly in the context of targeting it to other components of the nuclear protein import machinery to specify directionality of transport. The binding of GDP-Ran at the NTF2 cavity raises the possibility that this interaction might be modulated by a metabolite or small molecule substrate for NTF2's putative enzymatic activity.

-	~~{{STRUCTURE_1a2k\| PDB=1a2k \| SCENE= }}~~	+	Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran.,Stewart M, Kent HM, McCoy AJ J Mol Biol. 1998 Apr 3;277(3):635-46. PMID:9533885<ref>PMID:9533885</ref>

-	~~===GDPRAN-NTF2 COMPLEX===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9533885}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1a2k]] is~~ a ~~5 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en~~.~~wikipedia.org~~/~~wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:009533885<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Canis lupus familiaris]]		[[Category: Canis lupus familiaris]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]

OCA: Protected "1a2k" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 12:21:29 GMT

Protected "1a2k" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:21, 21 October 2012

OCA at 12:21, 21 October 2012

OCA — Sun, 21 Oct 2012 12:21:27 GMT

←Older revision		Revision as of 12:21, 21 October 2012
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-	{{Seed}}
	[[Image:1a2k.png\|left\|200px]]		[[Image:1a2k.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1a2k", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1a2k\| PDB=1a2k \| SCENE= }}		{{STRUCTURE_1a2k\| PDB=1a2k \| SCENE= }}

	===GDPRAN-NTF2 COMPLEX===		===GDPRAN-NTF2 COMPLEX===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9533885}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9533885 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9533885}}		{{ABSTRACT_PUBMED_9533885}}

	==About this Structure==		==About this Structure==
-	~~1A2K~~ is a 5 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA].	+	[[1a2k]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2K OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9533885~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009533885</ref><references group="xtra"/>
	[[Category: Canis lupus familiaris]]		[[Category: Canis lupus familiaris]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
Line 30:		Line 18:
	[[Category: Stewart, M.]]		[[Category: Stewart, M.]]
	[[Category: Gtp-binding]]		[[Category: Gtp-binding]]
-		+	[[Category: Transport-nuclear protein complex]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 04:53:05 2009''~~	+