1a87 - Revision history

OCA at 06:28, 7 February 2024

OCA — Wed, 07 Feb 2024 06:28:30 GMT

←Older revision		Revision as of 06:28, 7 February 2024
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	<StructureSection load='1a87' size='340' side='right'caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='1a87' size='340' side='right'caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A87 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A87 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [https://pdbe.org/1a87 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [https://www.ebi.ac.uk/pdbsum/1a87 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a87 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [https://pdbe.org/1a87 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [https://www.ebi.ac.uk/pdbsum/1a87 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a87 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CEAN_ECOLX CEAN_ECOLX]] This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.	+	[https://www.uniprot.org/uniprot/CEAN_ECOLX CEAN_ECOLX] This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a87 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a87 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Pore-forming colicins are water-soluble bacteriocins capable of binding to and translocating through the Escherichia coli cell envelope. They then undergo a transition to a transmembrane ion channel in the cytoplasmic membrane leading to bacterial death. Colicin N is the smallest pore-forming colicin known to date (40 kDa instead of the more usual 60 kDa) and the crystal structure of its membrane receptor, the porin OmpF, is already known. Structural knowledge of colicin N is therefore important for a molecular understanding of colicin toxicity and is relevant to toxic mechanisms in general. RESULTS: The crystal structure of colicin N reveals a novel receptor-binding domain containing a six-stranded antiparallel beta sheet wrapped around the 63 A long N-terminal alpha helix of the pore-forming domain. The pore-forming domain adopts a ten alpha-helix bundle that has been observed previously in the pore-forming domains of colicin A, la and E1. The translocation domain, however, does not appear to adopt any regular structure. Models for receptor binding and translocation through the outer membrane are proposed based on the structure and biochemical data. CONCLUSIONS: The colicin N-ompF system is now the structurally best-defined translocation pathway. Knowledge of the colicin N structure, coupled with the structure of its receptor, OmpF, and previously published biochemical data, limits the numerous possibilities of translocation and leads to a model in which the translocation domain inserts itself through the porin pore, the receptor-binding domain stays outside and the pore-forming domain translocates along the outer wall of the trimeric porin channel.
-
-	Crystal structure of a colicin N fragment suggests a model for toxicity.,Vetter IR, Parker MW, Tucker AD, Lakey JH, Pattus F, Tsernoglou D Structure. 1998 Jul 15;6(7):863-74. PMID:9687368<ref>PMID:9687368</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1a87" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Colicin 3D structures\|Colicin 3D structures]]		*[[Colicin 3D structures\|Colicin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lakey~~, J H~~]]	+	[[Category: Lakey JH]]
-	[[Category: Parker~~, M W~~]]	+	[[Category: Parker MW]]
-	[[Category: Pattus, F]]	+	[[Category: Pattus F]]
-	[[Category: Tsernoglou, D]]	+	[[Category: Tsernoglou D]]
-	[[Category: Tucker~~, A D~~]]	+	[[Category: Tucker AD]]
-	[[Category: Vetter~~, I R]]~~	+	[[Category: Vetter IR]]
-	~~[[Category: Bacteriocin]]~~	+
-	~~[[Category: Pore-forming activity]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 09:43, 26 May 2021

OCA — Wed, 26 May 2021 09:43:21 GMT

←Older revision		Revision as of 09:43, 26 May 2021
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	<StructureSection load='1a87' size='340' side='right'caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='1a87' size='340' side='right'caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1A87 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A87 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [~~http~~://pdbe.org/1a87 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1a87 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1a87 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [https://pdbe.org/1a87 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [https://www.ebi.ac.uk/pdbsum/1a87 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a87 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CEAN_ECOLX CEAN_ECOLX]] This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.	+	[[https://www.uniprot.org/uniprot/CEAN_ECOLX CEAN_ECOLX]] This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	==See Also==		==See Also==
	*[[Colicin 3D structures\|Colicin 3D structures]]		*[[Colicin 3D structures\|Colicin 3D structures]]
-	*[[Colicin N\|Colicin N]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:39, 21 August 2019

OCA — Wed, 21 Aug 2019 07:39:04 GMT

←Older revision		Revision as of 07:39, 21 August 2019
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	==COLICIN N==		==COLICIN N==
-	<StructureSection load='1a87' size='340' side='right' caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='1a87' size='340' side='right'caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A87 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A87 FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/1a87_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/1a87_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1a87" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1a87" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Colicin 3D structures\|Colicin 3D structures]]
		+	*[[Colicin N\|Colicin N]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Lakey, J H]]		[[Category: Lakey, J H]]
	[[Category: Parker, M W]]		[[Category: Parker, M W]]

OCA at 10:40, 15 November 2017

OCA — Wed, 15 Nov 2017 10:40:27 GMT

←Older revision		Revision as of 10:40, 15 November 2017
Line 1:		Line 1:
		+
	==COLICIN N==		==COLICIN N==
	<StructureSection load='1a87' size='340' side='right' caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='1a87' size='340' side='right' caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A87 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A87 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [http://pdbe.org/1a87 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [http://www.ebi.ac.uk/pdbsum/1a87 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [http://pdbe.org/1a87 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [http://www.ebi.ac.uk/pdbsum/1a87 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1a87 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1a87" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1a87" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Colicin\|Colicin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:01, 8 February 2016

OCA — Mon, 08 Feb 2016 10:01:58 GMT

←Older revision		Revision as of 10:01, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a87 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:53, 9 September 2015

OCA — Wed, 09 Sep 2015 15:53:23 GMT

←Older revision		Revision as of 15:53, 9 September 2015
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	<StructureSection load='1a87' size='340' side='right' caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='1a87' size='340' side='right' caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A87 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A87 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [http://www.ebi.ac.uk/pdbsum/1a87 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [http://pdbe.org/1a87 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [http://www.ebi.ac.uk/pdbsum/1a87 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1a87" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Lakey, J H]]		[[Category: Lakey, J H]]
	[[Category: Parker, M W]]		[[Category: Parker, M W]]

OCA at 22:31, 24 December 2014

OCA — Wed, 24 Dec 2014 22:31:38 GMT

←Older revision		Revision as of 22:31, 24 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [http://www.ebi.ac.uk/pdbsum/1a87 PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [http://www.ebi.ac.uk/pdbsum/1a87 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CEAN_ECOLX CEAN_ECOLX]] This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:09, 22 December 2014

OCA — Mon, 22 Dec 2014 09:09:44 GMT

←Older revision		Revision as of 09:09, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A87 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A87 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [http://www.ebi.ac.uk/pdbsum/1a87 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [http://www.ebi.ac.uk/pdbsum/1a87 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
-	[[Category: Lakey, J H.]]	+	[[Category: Lakey, J H]]
-	[[Category: Parker, M W.]]	+	[[Category: Parker, M W]]
-	[[Category: Pattus, F.]]	+	[[Category: Pattus, F]]
-	[[Category: Tsernoglou, D.]]	+	[[Category: Tsernoglou, D]]
-	[[Category: Tucker, A D.]]	+	[[Category: Tucker, A D]]
-	[[Category: Vetter, I R.]]	+	[[Category: Vetter, I R]]
	[[Category: Bacteriocin]]		[[Category: Bacteriocin]]
	[[Category: Pore-forming activity]]		[[Category: Pore-forming activity]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 08:31, 23 July 2014

OCA — Wed, 23 Jul 2014 08:31:49 GMT

←Older revision		Revision as of 08:31, 23 July 2014
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-	[[~~Image~~:1a87.~~png\|left~~\|200px]]	+	==COLICIN N==
		+	<StructureSection load='1a87' size='340' side='right' caption='[[1a87]], [[Resolution\|resolution]] 3.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A87 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a87 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a87 RCSB], [http://www.ebi.ac.uk/pdbsum/1a87 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/1a87_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Pore-forming colicins are water-soluble bacteriocins capable of binding to and translocating through the Escherichia coli cell envelope. They then undergo a transition to a transmembrane ion channel in the cytoplasmic membrane leading to bacterial death. Colicin N is the smallest pore-forming colicin known to date (40 kDa instead of the more usual 60 kDa) and the crystal structure of its membrane receptor, the porin OmpF, is already known. Structural knowledge of colicin N is therefore important for a molecular understanding of colicin toxicity and is relevant to toxic mechanisms in general. RESULTS: The crystal structure of colicin N reveals a novel receptor-binding domain containing a six-stranded antiparallel beta sheet wrapped around the 63 A long N-terminal alpha helix of the pore-forming domain. The pore-forming domain adopts a ten alpha-helix bundle that has been observed previously in the pore-forming domains of colicin A, la and E1. The translocation domain, however, does not appear to adopt any regular structure. Models for receptor binding and translocation through the outer membrane are proposed based on the structure and biochemical data. CONCLUSIONS: The colicin N-ompF system is now the structurally best-defined translocation pathway. Knowledge of the colicin N structure, coupled with the structure of its receptor, OmpF, and previously published biochemical data, limits the numerous possibilities of translocation and leads to a model in which the translocation domain inserts itself through the porin pore, the receptor-binding domain stays outside and the pore-forming domain translocates along the outer wall of the trimeric porin channel.

-	~~{{STRUCTURE_1a87\| PDB=1a87 \| SCENE= }}~~	+	Crystal structure of a colicin N fragment suggests a model for toxicity.,Vetter IR, Parker MW, Tucker AD, Lakey JH, Pattus F, Tsernoglou D Structure. 1998 Jul 15;6(7):863-74. PMID:9687368<ref>PMID:9687368</ref>

-	~~===COLICIN N===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9687368}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1a87]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA].~~	+

	==See Also==		==See Also==
	*[[Colicin\|Colicin]]		*[[Colicin\|Colicin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009687368<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Lakey, J H.]]		[[Category: Lakey, J H.]]

OCA at 09:47, 20 October 2012

OCA — Sat, 20 Oct 2012 09:47:35 GMT

←Older revision		Revision as of 09:47, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1a87]] is a 1 chain structure ~~of [[Colicin]]~~ with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k12~~ Escherichia coli ~~k12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA].	+	[[1a87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A87 OCA].

	==See Also==		==See Also==
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	==Reference==		==Reference==
	<ref group="xtra">PMID:009687368</ref><references group="xtra"/>		<ref group="xtra">PMID:009687368</ref><references group="xtra"/>
-	[[Category: Escherichia coli ~~k12~~]]	+	[[Category: Escherichia coli k-12]]
	[[Category: Lakey, J H.]]		[[Category: Lakey, J H.]]
	[[Category: Parker, M W.]]		[[Category: Parker, M W.]]