1ab0 - Revision history

OCA at 10:50, 2 August 2023

OCA — Wed, 02 Aug 2023 10:50:29 GMT

←Older revision		Revision as of 10:50, 2 August 2023
Line 3:		Line 3:
	<StructureSection load='1ab0' size='340' side='right'caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ab0' size='340' side='right'caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [https://pdbe.org/1ab0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ab0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab0 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [https://pdbe.org/1ab0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ab0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FABP4_MOUSE FABP4_MOUSE]] Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.<ref>PMID:12077340</ref> <ref>PMID:16574478</ref> <ref>PMID:17516629</ref>	+	[https://www.uniprot.org/uniprot/FABP4_MOUSE FABP4_MOUSE] Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.<ref>PMID:12077340</ref> <ref>PMID:16574478</ref> <ref>PMID:17516629</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Banaszak, L]]	+	[[Category: Banaszak L]]
-	[[Category: Bernlohr, D]]	+	[[Category: Bernlohr D]]
-	[[Category: Kane, C]]	+	[[Category: Kane C]]
-	[[Category: Ory, J]]	+	[[Category: Ory J]]
-	[[Category: Simpson, M]]	+	[[Category: Simpson M]]
-	~~[[Category: Fatty acid binding protein~~]]	+

OCA at 09:44, 26 May 2021

OCA — Wed, 26 May 2021 09:44:10 GMT

←Older revision		Revision as of 09:44, 26 May 2021
Line 3:		Line 3:
	<StructureSection load='1ab0' size='340' side='right'caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ab0' size='340' side='right'caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [~~http~~://pdbe.org/1ab0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ab0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab0 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [https://pdbe.org/1ab0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ab0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FABP4_MOUSE FABP4_MOUSE]] Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.<ref>PMID:12077340</ref> <ref>PMID:16574478</ref> <ref>PMID:17516629</ref>	+	[[https://www.uniprot.org/uniprot/FABP4_MOUSE FABP4_MOUSE]] Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.<ref>PMID:12077340</ref> <ref>PMID:16574478</ref> <ref>PMID:17516629</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:13, 21 August 2019

OCA — Wed, 21 Aug 2019 07:13:22 GMT

←Older revision		Revision as of 07:13, 21 August 2019
Line 1:		Line 1:

	==C1G/V32D/F57H MUTANT OF MURINE ADIPOCYTE LIPID BINDING PROTEIN AT PH 4.5==		==C1G/V32D/F57H MUTANT OF MURINE ADIPOCYTE LIPID BINDING PROTEIN AT PH 4.5==
-	<StructureSection load='1ab0' size='340' side='right' caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1ab0' size='340' side='right'caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/1ab0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/1ab0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1ab0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ab0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Fatty acid-binding protein 3D structures\|Fatty acid-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Banaszak, L]]		[[Category: Banaszak, L]]

OCA at 10:40, 15 November 2017

OCA — Wed, 15 Nov 2017 10:40:44 GMT

←Older revision		Revision as of 10:40, 15 November 2017
Line 1:		Line 1:
		+
	==C1G/V32D/F57H MUTANT OF MURINE ADIPOCYTE LIPID BINDING PROTEIN AT PH 4.5==		==C1G/V32D/F57H MUTANT OF MURINE ADIPOCYTE LIPID BINDING PROTEIN AT PH 4.5==
	<StructureSection load='1ab0' size='340' side='right' caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ab0' size='340' side='right' caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [http://pdbe.org/1ab0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab0 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [http://pdbe.org/1ab0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1ab0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ab0" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Fatty acid-binding protein\|Fatty acid-binding protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:10, 10 February 2016

OCA — Wed, 10 Feb 2016 01:10:51 GMT

←Older revision		Revision as of 01:10, 10 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ab0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:52, 11 September 2015

OCA — Fri, 11 Sep 2015 03:52:41 GMT

←Older revision		Revision as of 03:52, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ab0' size='340' side='right' caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ab0' size='340' side='right' caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab0 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [http://pdbe.org/1ab0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ab0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Banaszak, L]]		[[Category: Banaszak, L]]
	[[Category: Bernlohr, D]]		[[Category: Bernlohr, D]]

OCA at 10:44, 25 December 2014

OCA — Thu, 25 Dec 2014 10:44:29 GMT

←Older revision		Revision as of 10:44, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab0 PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab0 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FABP4_MOUSE FABP4_MOUSE]] Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.<ref>PMID:12077340</ref> <ref>PMID:16574478</ref> <ref>PMID:17516629</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:33, 22 December 2014

OCA — Mon, 22 Dec 2014 11:33:46 GMT

←Older revision		Revision as of 11:33, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab0 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab0 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Banaszak, L.]]	+	[[Category: Banaszak, L]]
-	[[Category: Bernlohr, D.]]	+	[[Category: Bernlohr, D]]
-	[[Category: Kane, C.]]	+	[[Category: Kane, C]]
-	[[Category: Ory, J.]]	+	[[Category: Ory, J]]
-	[[Category: Simpson, M.]]	+	[[Category: Simpson, M]]
	[[Category: Fatty acid binding protein]]		[[Category: Fatty acid binding protein]]

OCA at 08:31, 23 July 2014

OCA — Wed, 23 Jul 2014 08:31:59 GMT

←Older revision		Revision as of 08:31, 23 July 2014
Line 1:		Line 1:
-	[[~~Image~~:1ab0.~~png\|left~~\|200px]]	+	==C1G/V32D/F57H MUTANT OF MURINE ADIPOCYTE LIPID BINDING PROTEIN AT PH 4.5==
		+	<StructureSection load='1ab0' size='340' side='right' caption='[[1ab0]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ab0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab0 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/1ab0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A number of crystallographic studies of the adipocyte lipid-binding protein have established that the fatty acid-binding site is within an internalized water-filled cavity. The same studies have also suggested the existence of a region physically distinct from the fatty acid-binding site which connects the cavity of the protein with the external solvent, hereafter referred to as the portal. In an effort to examine the portal region, we have used site-directed mutagenesis to introduce the mutations V32D/F57H into the murine ALBP cDNA. Mutant protein has been isolated, crystallized, and its stability and binding properties studied by biochemical methods. As assessed by guanidine-HCl denaturation, the mutant form exhibited a slight overall destabilization relative to the wild-type protein under both acid and alkaline conditions. Accessibility to the cavity in both the mutant and wild-type proteins was observed by stopped-flow analysis of the modification of a cavity residue, Cys117, by the sulfhydryl reactive agent 5, 5'-dithiobis(2-nitrobenzoic acid) at pH 8.5. Cys117 of V32D/F57H ALBP was modified 7-fold faster than the wild-type protein. The ligand binding properties of both the V32D/F57H mutant and wild-type proteins were analyzed using a fluorescent probe at pH 6.0 and 8.0. The apparent dissociation constants for 1-anilinonaphthalene-8-sulfonic acid were approximately 9-10-fold greater than the wild-type protein, independent of pH. In addition, there is a 6-fold increase in the Kd for oleic acid for the portal mutant relative to the wild-type at pH 8.0. To study the effect of pH on the double mutant, it was crystallized and analyzed in two distinct space groups at pH 4.5 and 6.4. While in general the differences in the overall main chain conformations are negligible, changes were observed in the crystallographic structures near the site of the mutations. At both pH values, the mutant side chains are positioned somewhat differently than in wild-type protein. To ensure that the mutations had not altered ionic conditions near the binding site, the crystallographic coordinates were used to monitor the electrostatic potentials from the head group site to the positions near the portal region. The differences in the electrostatic potentials were small in all regions, and did not explain the differences in ligand affinity. We present these results within the context of fatty acid binding and suggest lipid association is more complex than that described within a single equilibrium event.

-	~~{{STRUCTURE_1ab0\| PDB=1ab0 \| SCENE= }}~~	+	Biochemical and crystallographic analyses of a portal mutant of the adipocyte lipid-binding protein.,Ory J, Kane CD, Simpson MA, Banaszak LJ, Bernlohr DA J Biol Chem. 1997 Apr 11;272(15):9793-801. PMID:9092513<ref>PMID:9092513</ref>

-	~~===C1G~~/~~V32D/F57H MUTANT OF MURINE ADIPOCYTE LIPID BINDING PROTEIN AT PH 4~~.~~5===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_9092513}}~~	+	==See Also==
-		+	*[[Fatty acid-binding protein\|Fatty acid-binding protein]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1ab0~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB0 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:009092513<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Banaszak, L.]]		[[Category: Banaszak, L.]]

OCA: Protected "1ab0" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 11:52:18 GMT

Protected "1ab0" [edit=sysop:move=sysop]

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Revision as of 11:52, 21 October 2012