1ab5 - Revision history

OCA at 10:50, 2 August 2023

OCA — Wed, 02 Aug 2023 10:50:31 GMT

←Older revision		Revision as of 10:50, 2 August 2023
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	<StructureSection load='1ab5' size='340' side='right'caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ab5' size='340' side='right'caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [https://pdbe.org/1ab5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ab5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab5 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [https://pdbe.org/1ab5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ab5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>	+	[https://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Coll, M]]	+	[[Category: Coll M]]
-	[[Category: Lopez-Hernandez, E]]	+	[[Category: Lopez-Hernandez E]]
-	[[Category: Pisabarro~~, M T~~]]	+	[[Category: Pisabarro MT]]
-	[[Category: Serrano, L]]	+	[[Category: Serrano L]]
-	[[Category: Wilcock, D]]	+	[[Category: Wilcock D]]
-	~~[[Category: Chemotaxis]]~~	+
-	~~[[Category: Flagellar rot]]~~	+
-	~~[[Category: Phosphorylation]]~~	+
-	~~[[Category: Sensory transduction~~]]	+

OCA at 09:44, 26 May 2021

OCA — Wed, 26 May 2021 09:44:11 GMT

←Older revision		Revision as of 09:44, 26 May 2021
Line 3:		Line 3:
	<StructureSection load='1ab5' size='340' side='right'caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ab5' size='340' side='right'caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [~~http~~://pdbe.org/1ab5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ab5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab5 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [https://pdbe.org/1ab5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ab5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>	+	[[https://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:49, 21 August 2019

OCA — Wed, 21 Aug 2019 07:49:56 GMT

←Older revision		Revision as of 07:49, 21 August 2019
Line 1:		Line 1:

	==STRUCTURE OF CHEY MUTANT F14N, V21T==		==STRUCTURE OF CHEY MUTANT F14N, V21T==
-	<StructureSection load='1ab5' size='340' side='right' caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='1ab5' size='340' side='right'caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/1ab5_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/1ab5_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Coll, M]]		[[Category: Coll, M]]
	[[Category: Lopez-Hernandez, E]]		[[Category: Lopez-Hernandez, E]]

OCA at 10:48, 15 November 2017

OCA — Wed, 15 Nov 2017 10:48:12 GMT

←Older revision		Revision as of 10:48, 15 November 2017
Line 1:		Line 1:
		+
	==STRUCTURE OF CHEY MUTANT F14N, V21T==		==STRUCTURE OF CHEY MUTANT F14N, V21T==
	<StructureSection load='1ab5' size='340' side='right' caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ab5' size='340' side='right' caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [http://pdbe.org/1ab5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab5 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [http://pdbe.org/1ab5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ab5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 00:17, 9 February 2016

OCA — Tue, 09 Feb 2016 00:17:07 GMT

←Older revision		Revision as of 00:17, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ab5 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:45, 10 September 2015

OCA — Thu, 10 Sep 2015 16:45:20 GMT

←Older revision		Revision as of 16:45, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1ab5' size='340' side='right' caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ab5' size='340' side='right' caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab5 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [http://pdbe.org/1ab5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab5 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ab5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Coll, M]]		[[Category: Coll, M]]
	[[Category: Lopez-Hernandez, E]]		[[Category: Lopez-Hernandez, E]]

OCA at 03:08, 25 December 2014

OCA — Thu, 25 Dec 2014 03:08:48 GMT

←Older revision		Revision as of 03:08, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab5 PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab5 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:22, 22 December 2014

OCA — Mon, 22 Dec 2014 09:22:53 GMT

←Older revision		Revision as of 09:22, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab5 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab5 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Coll, M.]]	+	[[Category: Coll, M]]
-	[[Category: Lopez-Hernandez, E.]]	+	[[Category: Lopez-Hernandez, E]]
-	[[Category: Pisabarro, M T.]]	+	[[Category: Pisabarro, M T]]
-	[[Category: Serrano, L.]]	+	[[Category: Serrano, L]]
-	[[Category: Wilcock, D.]]	+	[[Category: Wilcock, D]]
	[[Category: Chemotaxis]]		[[Category: Chemotaxis]]
	[[Category: Flagellar rot]]		[[Category: Flagellar rot]]
	[[Category: Phosphorylation]]		[[Category: Phosphorylation]]
	[[Category: Sensory transduction]]		[[Category: Sensory transduction]]

OCA at 08:37, 23 July 2014

OCA — Wed, 23 Jul 2014 08:37:20 GMT

←Older revision		Revision as of 08:37, 23 July 2014
Line 1:		Line 1:
-	[[~~Image~~:1ab5.~~png\|left~~\|200px]]	+	==STRUCTURE OF CHEY MUTANT F14N, V21T==
		+	<StructureSection load='1ab5' size='340' side='right' caption='[[1ab5]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AB5 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ab5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ab5 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/1ab5_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structures of two double mutants (F14N/V21T and F14N/V86T) of the signal transduction protein CheY have been determined to a resolution of 2.4 and 2.2 A, respectively. The structures were solved by molecular replacement and refined to final R values of 18.4 and 19.2%, respectively. Together with urea-denaturation experiments the structures have been used to analyse the effects of mutations where hydrophobic residues are replaced by residues capable of establishing hydrogen bonds. The large increase in stabilization (-12.1 kJ mol-1) of the mutation Phe14Asn arises from two factors: a reverse hydrophobic effect and the formation of a good N-cap at alpha-helix 1. In addition, a forward-backward hydrogen-bonding pattern, resembling an N-capping box and involving Asn14 and Arg18, has been found. The two Val to Thr mutations at the hydrophobic core have different thermodynamic effects: the mutation Val21Thr does not affect the stability of the protein while the mutation Val86Thr causes a small destabilization of 1.7 kJ mol-1. At site 21 a backward side chain-to-backbone hydrogen bond is formed inside alpha-helix 1 with the carbonyl O atom of the i - 4 residue without movement of the mutated side chain. The destabilizing effect of introducing a polar group in the core is efficiently compensated for by the formation of an extra hydrogen bond. At site 86 the new Ogamma atom escapes from the hydrophobic environment by a chi1 rotation into an adjacent hydrophilic cavity to form a new hydrogen bond. In this case the isosteric Val to Thr substitution is disruptive but the loss in stabilization energy is partly compensated by the formation of a hydrogen bond. The two crystal structures described in this work underline the significance of the hydrogen-bond component to protein stability.

-	~~{{STRUCTURE_1ab5\| PDB=1ab5 \| SCENE= }}~~	+	Structure analysis of two CheY mutants: importance of the hydrogen-bond contribution to protein stability.,Wilcock D, Pisabarro MT, Lopez-Hernandez E, Serrano L, Coll M Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):378-85. PMID:9761905<ref>PMID:9761905</ref>

-	~~===STRUCTURE OF CHEY MUTANT F14N~~, ~~V21T===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9761905}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ab5]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1AB5 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:009761905<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Coll, M.]]		[[Category: Coll, M.]]

OCA: Protected "1ab5" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 12:22:35 GMT

Protected "1ab5" [edit=sysop:move=sysop]

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Revision as of 12:22, 21 October 2012