1ae9 - Revision history

OCA at 06:30, 7 February 2024

OCA — Wed, 07 Feb 2024 06:30:00 GMT

←Older revision		Revision as of 06:30, 7 February 2024
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	<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacteriophage_lambda Bacteriophage lambda~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [https://pdbe.org/1ae9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [https://pdbe.org/1ae9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD]] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.	+	[https://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae9 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Lambda integrase is archetypic of site-specific recombinases that catalyze intermolecular DNA rearrangements without energetic input. DNA cleavage, strand exchange, and religation steps are linked by a covalent phosphotyrosine intermediate in which Tyr342 is attached to the 3'-phosphate of the DNA cut site. The 1.9 angstrom crystal structure of the integrase catalytic domain reveals a protein fold that is conserved in organisms ranging from archaebacteria to yeast and that suggests a model for interaction with target DNA. The attacking Tyr342 nucleophile is located on a flexible loop about 20 angstroms from a basic groove that contains all the other catalytically essential residues. This bipartite active site can account for several apparently paradoxical features of integrase family recombinases, including the capacity for both cis and trans cleavage of DNA.
-
-	Flexibility in DNA recombination: structure of the lambda integrase catalytic core.,Kwon HJ, Tirumalai R, Landy A, Ellenberger T Science. 1997 Apr 4;276(5309):126-31. PMID:9082984<ref>PMID:9082984</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ae9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Retroviral integrase 3D structures\|Retroviral integrase 3D structures]]		*[[Retroviral integrase 3D structures\|Retroviral integrase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacteriophage lambda~~]]	+	[[Category: Escherichia virus Lambda]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ellenberger, T]]	+	[[Category: Ellenberger T]]
-	[[Category: Kwon~~, H J~~]]	+	[[Category: Kwon HJ]]
-	[[Category: Landy, A]]	+	[[Category: Landy A]]
-	[[Category: Tirumalai, R]]	+	[[Category: Tirumalai R]]
-	~~[[Category: Dna recombination]]~~	+
-	~~[[Category: Integrase]]~~	+
-	~~[[Category: Site-specific recombination~~]]	+

OCA at 14:38, 3 November 2021

OCA — Wed, 03 Nov 2021 14:38:16 GMT

←Older revision		Revision as of 14:38, 3 November 2021
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	<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [https://pdbe.org/1ae9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [https://pdbe.org/1ae9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacteriophage lambda]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ellenberger, T]]		[[Category: Ellenberger, T]]

OCA at 10:19, 17 February 2021

OCA — Wed, 17 Feb 2021 10:19:26 GMT

←Older revision		Revision as of 10:19, 17 February 2021
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	<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [~~http~~://pdbe.org/1ae9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [https://pdbe.org/1ae9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD]] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.	+	[[https://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD]] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Retroviral ~~Integrase~~\|Retroviral ~~Integrase~~]]	+	*[[Retroviral integrase 3D structures\|Retroviral integrase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:57, 10 May 2019

OCA — Fri, 10 May 2019 13:57:05 GMT

←Older revision		Revision as of 13:57, 10 May 2019
Line 1:		Line 1:

	==STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE==		==STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE==
-	<StructureSection load='1ae9' size='340' side='right' caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[~~Phage integrase~~\|~~Phage integrase]]~~	+	*[[Retroviral Integrase\|Retroviral Integrase]]
-	*[[Resolvase\|Resolvase]]	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ellenberger, T]]		[[Category: Ellenberger, T]]
	[[Category: Kwon, H J]]		[[Category: Kwon, H J]]

OCA at 09:10, 9 August 2017

OCA — Wed, 09 Aug 2017 09:10:10 GMT

←Older revision		Revision as of 09:10, 9 August 2017
Line 1:		Line 1:
		+
	==STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE==		==STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE==
	<StructureSection load='1ae9' size='340' side='right' caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ae9' size='340' side='right' caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [http://pdbe.org/1ae9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae9 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [http://pdbe.org/1ae9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacteriophage lambda]]
	[[Category: Ellenberger, T]]		[[Category: Ellenberger, T]]
	[[Category: Kwon, H J]]		[[Category: Kwon, H J]]

OCA at 11:09, 11 September 2015

OCA — Fri, 11 Sep 2015 11:09:34 GMT

←Older revision		Revision as of 11:09, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ae9' size='340' side='right' caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ae9' size='340' side='right' caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_lambda Enterobacteria phage~~ lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae9 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [http://pdbe.org/1ae9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae9 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ae9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage~~ lambda]]	+	[[Category: Bacteriophage lambda]]
	[[Category: Ellenberger, T]]		[[Category: Ellenberger, T]]
	[[Category: Kwon, H J]]		[[Category: Kwon, H J]]

OCA at 15:38, 25 December 2014

OCA — Thu, 25 Dec 2014 15:38:42 GMT

←Older revision		Revision as of 15:38, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae9 PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae9 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD]] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:30, 22 December 2014

OCA — Mon, 22 Dec 2014 09:30:46 GMT

←Older revision		Revision as of 09:30, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae9 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae9 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
-	[[Category: Ellenberger, T.]]	+	[[Category: Ellenberger, T]]
-	[[Category: Kwon, H J.]]	+	[[Category: Kwon, H J]]
-	[[Category: Landy, A.]]	+	[[Category: Landy, A]]
-	[[Category: Tirumalai, R.]]	+	[[Category: Tirumalai, R]]
	[[Category: Dna recombination]]		[[Category: Dna recombination]]
	[[Category: Integrase]]		[[Category: Integrase]]
	[[Category: Site-specific recombination]]		[[Category: Site-specific recombination]]

OCA at 08:06, 30 July 2014

OCA — Wed, 30 Jul 2014 08:06:00 GMT

←Older revision		Revision as of 08:06, 30 July 2014
Line 1:		Line 1:
-	[[~~Image~~:1ae9.~~png\|left~~\|200px]]	+	==STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE==
		+	<StructureSection load='1ae9' size='340' side='right' caption='[[1ae9]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lambda integrase is archetypic of site-specific recombinases that catalyze intermolecular DNA rearrangements without energetic input. DNA cleavage, strand exchange, and religation steps are linked by a covalent phosphotyrosine intermediate in which Tyr342 is attached to the 3'-phosphate of the DNA cut site. The 1.9 angstrom crystal structure of the integrase catalytic domain reveals a protein fold that is conserved in organisms ranging from archaebacteria to yeast and that suggests a model for interaction with target DNA. The attacking Tyr342 nucleophile is located on a flexible loop about 20 angstroms from a basic groove that contains all the other catalytically essential residues. This bipartite active site can account for several apparently paradoxical features of integrase family recombinases, including the capacity for both cis and trans cleavage of DNA.

-	~~{{STRUCTURE_1ae9\| PDB=1ae9 \| SCENE= }}~~	+	Flexibility in DNA recombination: structure of the lambda integrase catalytic core.,Kwon HJ, Tirumalai R, Landy A, Ellenberger T Science. 1997 Apr 4;276(5309):126-31. PMID:9082984<ref>PMID:9082984</ref>

-	~~===STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9082984}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1ae9]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA].~~	+

	==See Also==		==See Also==
	*[[Phage integrase\|Phage integrase]]		*[[Phage integrase\|Phage integrase]]
	*[[Resolvase\|Resolvase]]		*[[Resolvase\|Resolvase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009082984<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
	[[Category: Ellenberger, T.]]		[[Category: Ellenberger, T.]]

OCA at 18:26, 20 October 2012

OCA — Sat, 20 Oct 2012 18:26:48 GMT

←Older revision		Revision as of 18:26, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1ae9]] is a 2 chain structure ~~of [[Phage integrase]] and [[Resolvase]]~~ with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA].	+	[[1ae9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA].

	==See Also==		==See Also==