1af8 - Revision history

OCA at 15:23, 13 March 2024

OCA — Wed, 13 Mar 2024 15:23:33 GMT

←Older revision		Revision as of 15:23, 13 March 2024
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	==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, 24 STRUCTURES==		==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, 24 STRUCTURES==
-	<StructureSection load='1af8' size='340' side='right'caption='[[1af8~~]], [[NMR_Ensembles_of_Models \| 24 NMR models~~]]' scene=''>	+	<StructureSection load='1af8' size='340' side='right'caption='[[1af8]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1af8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strco Strco~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1AF8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1af8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AF8 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[2af8\|2af8]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [https://pdbe.org/1af8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [https://www.ebi.ac.uk/pdbsum/1af8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1af8 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [~~http~~://pdbe.org/1af8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1af8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1af8 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO]] Acyl carrier protein.	+	[https://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO] Acyl carrier protein.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1af8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1af8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from the polyketide synthase (PKS) of Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing the first polyketide synthase component for which detailed structural information has been obtained. Twenty-four structures were generated by simulated annealing, employing 699 distance restraints and 94 dihedral angle restraints. The structure is composed, principally, of three major helices (1, 2, and 4), a shorter helix (3) and a large loop region separating helices 1 and 2. The structure is well-defined, except for a portion of the loop region (residues 18-29), the N-terminus (1-4), and a short stretch (57-61) in the loop connecting helices 2 and 3. The RMS distribution of the 24 structures about the average structure is 1.47 A for backbone atoms, 1.84 A for all heavy atoms (residues 5-86), and 1.01 A for backbone atoms over the helical regions (5-18, 41-86). The tertiary fold of act apo-ACP shows a strong structural homology with Escherichia coli fatty acid synthase (FAS) ACP, though some structural differences exist. First, there is no evidence that act apo-ACP is conformationally averaged between two or more states as observed in E. coli FAS ACP. Second, act apo-ACP shows a disordered N-terminus (residues 1-4) and a longer flexible loop (19-41 with 19-29 disordered) as opposed to E. coli FAS ACP where the N-terminal helix starts at residue 3 and the loop region is three amino acids shorter (16-35). Most importantly, however, although the act apo-ACP structure contains a hydrophobic core, there are in addition a number of buried hydrophilic groups, principally Arg72 and Asn79, both of which are 100% conserved in the PKS ACPs and not the FAS ACPs and may therefore play a role in stabilizing the growing polyketide chain. The structure-function relationship of act ACP is discussed in the light of these structural data and recent genetic advances in the field.
-
-	Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2).,Crump MP, Crosby J, Dempsey CE, Parkinson JA, Murray M, Hopwood DA, Simpson TJ Biochemistry. 1997 May 20;36(20):6000-8. PMID:9166770<ref>PMID:9166770</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1af8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]		*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Strco]]~~	+	[[Category: Crosby J]]
-	[[Category: Crosby, J]]	+	[[Category: Crump MP]]
-	[[Category: Crump~~, M P~~]]	+	[[Category: Dempsey CE]]
-	[[Category: Dempsey~~, C E~~]]	+	[[Category: Hopwood DA]]
-	[[Category: Hopwood~~, D A~~]]	+	[[Category: Murray M]]
-	[[Category: Murray, M]]	+	[[Category: Parkinson JA]]
-	[[Category: Parkinson~~, J A~~]]	+	[[Category: Simpson TJ]]
-	[[Category: Simpson~~, T J]]~~	+
-	~~[[Category: Acyl carrier protein]]~~	+
-	~~[[Category: Antibiotic biosynthesis]]~~	+
-	~~[[Category: Polyketide]]~~	+
-	~~[[Category: Solution structure]]~~	+
-	~~[[Category: Streptomyce~~]]	+

OCA at 10:09, 18 November 2020

OCA — Wed, 18 Nov 2020 10:09:02 GMT

←Older revision		Revision as of 10:09, 18 November 2020
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	==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, 24 STRUCTURES==		==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, 24 STRUCTURES==
-	<StructureSection load='1af8' size='340' side='right' caption='[[1af8]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>	+	<StructureSection load='1af8' size='340' side='right'caption='[[1af8]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AF8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1AF8 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2af8\|2af8]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2af8\|2af8]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://pdbe.org/1af8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1af8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://pdbe.org/1af8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1af8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/1af8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/1af8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1af8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1af8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Strco]]		[[Category: Strco]]
	[[Category: Crosby, J]]		[[Category: Crosby, J]]

OCA at 10:40, 15 November 2017

OCA — Wed, 15 Nov 2017 10:40:28 GMT

←Older revision		Revision as of 10:40, 15 November 2017
Line 1:		Line 1:
		+
	==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, 24 STRUCTURES==		==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, 24 STRUCTURES==
	<StructureSection load='1af8' size='340' side='right' caption='[[1af8]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>		<StructureSection load='1af8' size='340' side='right' caption='[[1af8]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2af8\|2af8]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2af8\|2af8]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://pdbe.org/1af8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://pdbe.org/1af8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1af8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1af8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1af8" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:39, 9 February 2016

OCA — Tue, 09 Feb 2016 22:39:55 GMT

←Older revision		Revision as of 22:39, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1af8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:44, 11 September 2015

OCA — Fri, 11 Sep 2015 02:44:52 GMT

←Older revision		Revision as of 02:44, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1af8' size='340' side='right' caption='[[1af8]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>		<StructureSection load='1af8' size='340' side='right' caption='[[1af8]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_coelicolor Streptomyces coelicolor~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AF8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AF8 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2af8\|2af8]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2af8\|2af8]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1902 Streptomyces coelicolor~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://pdbe.org/1af8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1af8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces coelicolor~~]]	+	[[Category: Strco]]
	[[Category: Crosby, J]]		[[Category: Crosby, J]]
	[[Category: Crump, M P]]		[[Category: Crump, M P]]

OCA at 10:02, 25 December 2014

OCA — Thu, 25 Dec 2014 10:02:16 GMT

←Older revision		Revision as of 10:02, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO]] Acyl carrier protein.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:08, 22 December 2014

OCA — Mon, 22 Dec 2014 10:08:33 GMT

←Older revision		Revision as of 10:08, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AF8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AF8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2af8\|2af8]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2af8\|2af8]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
-	[[Category: Crosby, J.]]	+	[[Category: Crosby, J]]
-	[[Category: Crump, M P.]]	+	[[Category: Crump, M P]]
-	[[Category: Dempsey, C E.]]	+	[[Category: Dempsey, C E]]
-	[[Category: Hopwood, D A.]]	+	[[Category: Hopwood, D A]]
-	[[Category: Murray, M.]]	+	[[Category: Murray, M]]
-	[[Category: Parkinson, J A.]]	+	[[Category: Parkinson, J A]]
-	[[Category: Simpson, T J.]]	+	[[Category: Simpson, T J]]
	[[Category: Acyl carrier protein]]		[[Category: Acyl carrier protein]]
	[[Category: Antibiotic biosynthesis]]		[[Category: Antibiotic biosynthesis]]

OCA at 08:10, 30 July 2014

OCA — Wed, 30 Jul 2014 08:10:51 GMT

←Older revision		Revision as of 08:10, 30 July 2014
Line 1:		Line 1:
-	[[~~Image:~~1af8.~~png~~\|~~left~~\|200px]]	+	==ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2), NMR, 24 STRUCTURES==
		+	<StructureSection load='1af8' size='340' side='right' caption='[[1af8]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AF8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2af8\|2af8]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACTINORHODIN ACYL CARRIER PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1af8 RCSB], [http://www.ebi.ac.uk/pdbsum/1af8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/1af8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from the polyketide synthase (PKS) of Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing the first polyketide synthase component for which detailed structural information has been obtained. Twenty-four structures were generated by simulated annealing, employing 699 distance restraints and 94 dihedral angle restraints. The structure is composed, principally, of three major helices (1, 2, and 4), a shorter helix (3) and a large loop region separating helices 1 and 2. The structure is well-defined, except for a portion of the loop region (residues 18-29), the N-terminus (1-4), and a short stretch (57-61) in the loop connecting helices 2 and 3. The RMS distribution of the 24 structures about the average structure is 1.47 A for backbone atoms, 1.84 A for all heavy atoms (residues 5-86), and 1.01 A for backbone atoms over the helical regions (5-18, 41-86). The tertiary fold of act apo-ACP shows a strong structural homology with Escherichia coli fatty acid synthase (FAS) ACP, though some structural differences exist. First, there is no evidence that act apo-ACP is conformationally averaged between two or more states as observed in E. coli FAS ACP. Second, act apo-ACP shows a disordered N-terminus (residues 1-4) and a longer flexible loop (19-41 with 19-29 disordered) as opposed to E. coli FAS ACP where the N-terminal helix starts at residue 3 and the loop region is three amino acids shorter (16-35). Most importantly, however, although the act apo-ACP structure contains a hydrophobic core, there are in addition a number of buried hydrophilic groups, principally Arg72 and Asn79, both of which are 100% conserved in the PKS ACPs and not the FAS ACPs and may therefore play a role in stabilizing the growing polyketide chain. The structure-function relationship of act ACP is discussed in the light of these structural data and recent genetic advances in the field.

-	~~{{STRUCTURE_1af8\| PDB=1af8 \| SCENE= }}~~	+	Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2).,Crump MP, Crosby J, Dempsey CE, Parkinson JA, Murray M, Hopwood DA, Simpson TJ Biochemistry. 1997 May 20;36(20):6000-8. PMID:9166770<ref>PMID:9166770</ref>

-	~~===ACTINORHODIN POLYKETIDE SYNTHASE ACYL CARRIER PROTEIN FROM STREPTOMYCES COELICOLOR A3(2)~~, ~~NMR, 24 STRUCTURES===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9166770}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1af8]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA].~~	+

	==See Also==		==See Also==
	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]		*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009166770<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Crosby, J.]]		[[Category: Crosby, J.]]

OCA at 15:23, 20 October 2012

OCA — Sat, 20 Oct 2012 15:23:44 GMT

←Older revision		Revision as of 15:23, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1af8]] is a 1 chain structure ~~of [[Acyl carrier protein synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA].	+	[[1af8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF8 OCA].

	==See Also==		==See Also==

OCA: Protected "1af8" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 18:15:09 GMT

Protected "1af8" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:15, 26 July 2012