1ahc - Revision history

OCA at 06:30, 7 February 2024

OCA — Wed, 07 Feb 2024 06:30:36 GMT

←Older revision		Revision as of 06:30, 7 February 2024
Line 3:		Line 3:
	<StructureSection load='1ahc' size='340' side='right'caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ahc' size='340' side='right'caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AHC FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>~~[https://en.wikipedia.org/wiki/rRNA_N~~-~~glycosylase rRNA N-glycosylase]~~, ~~with EC number~~ [~~https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.~~2~~.2.22 3.2.2.22] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [https://pdbe.org/1ahc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [https://www.ebi.ac.uk/pdbsum/1ahc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ahc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [https://pdbe.org/1ahc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [https://www.ebi.ac.uk/pdbsum/1ahc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ahc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RIP1_MOMCH RIP1_MOMCH]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ahc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ahc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: alpha-Momorcharin (alpha MMC) is a type I ribosome-inactivating protein. It inhibits protein synthesis by hydrolytically removing a specific adenine residue from a highly conserved, single-stranded loop of rRNA. RESULTS: Here we describe the determination and refinement of the crystal structures of alpha MMC in the native state and in complexes with the product, adenine, and a substrate analogue, formycin 5'-monophosphate (FMP) at high resolution. Both adenine and the base of FMP are tightly bound; the ribose of bound FMP adopts a strained, high-energy conformation, which may mimic the structure of the transition state. CONCLUSIONS: These structures indicate that residues Tyr70, Glu160 and Arg163 of alpha MMC are the most critical for catalysis. We propose that the strained conformation of the ribose in the target adenosine weakens the glycoside bond. Partial protonation mediated by Arg163 then facilitates N-glycoside bond cleavage, leading to the formation of an oxycarbonium ion intermediate which is stabilized by the negatively-charged Glu160. Tyr70 adopts subtly different conformations in the three structures implying that it may be important in substrate recognition and perhaps catalysis.
-
-	The N-glycosidase mechanism of ribosome-inactivating proteins implied by crystal structures of alpha-momorcharin.,Ren J, Wang Y, Dong Y, Stuart DI Structure. 1994 Jan 15;2(1):7-16. PMID:8075985<ref>PMID:8075985</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ahc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Ribosome inactivating protein\|Ribosome inactivating protein]]	+	*[[Ribosome inactivating protein 3D structures\|Ribosome inactivating protein 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~RRNA N-glycosylase~~]]	+	[[Category: Momordica charantia]]
-	[[Category: Dong, Y]]	+	[[Category: Dong Y]]
-	[[Category: Ren, J]]	+	[[Category: Ren J]]
-	[[Category: Stuart~~, D I~~]]	+	[[Category: Stuart DI]]
-	[[Category: Wang, Y]]	+	[[Category: Wang Y]]
-	~~[[Category: Glycosidase~~]]	+

OCA at 10:20, 17 February 2021

OCA — Wed, 17 Feb 2021 10:20:44 GMT

←Older revision		Revision as of 10:20, 17 February 2021
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	<StructureSection load='1ahc' size='340' side='right'caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ahc' size='340' side='right'caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AHC FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [~~http~~://pdbe.org/1ahc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ahc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ahc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [https://pdbe.org/1ahc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [https://www.ebi.ac.uk/pdbsum/1ahc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ahc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 14:44, 10 May 2019

OCA — Fri, 10 May 2019 14:44:06 GMT

←Older revision		Revision as of 14:44, 10 May 2019
Line 1:		Line 1:

	==THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN==		==THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN==
-	<StructureSection load='1ahc' size='340' side='right' caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1ahc' size='340' side='right'caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHC FirstGlance]. <br>
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/1ahc_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/1ahc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Dong, Y]]		[[Category: Dong, Y]]

OCA at 09:12, 9 August 2017

OCA — Wed, 09 Aug 2017 09:12:30 GMT

←Older revision		Revision as of 09:12, 9 August 2017
Line 1:		Line 1:
		+
	==THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN==		==THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN==
	<StructureSection load='1ahc' size='340' side='right' caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ahc' size='340' side='right' caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Balsam_pear Balsam pear]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHC FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [http://pdbe.org/1ahc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [http://www.ebi.ac.uk/pdbsum/1ahc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [http://pdbe.org/1ahc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [http://www.ebi.ac.uk/pdbsum/1ahc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ahc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ahc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Balsam pear]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Dong, Y]]		[[Category: Dong, Y]]

OCA at 22:18, 11 September 2015

OCA — Fri, 11 Sep 2015 22:18:45 GMT

←Older revision		Revision as of 22:18, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ahc' size='340' side='right' caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ahc' size='340' side='right' caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Momordica_charantia Momordica charantia~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHC FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [http://www.ebi.ac.uk/pdbsum/1ahc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [http://pdbe.org/1ahc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [http://www.ebi.ac.uk/pdbsum/1ahc PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ahc" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribosome inactivating protein\|Ribosome inactivating protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Momordica charantia~~]]	+	[[Category: Balsam pear]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Dong, Y]]		[[Category: Dong, Y]]

OCA at 11:02, 22 December 2014

OCA — Mon, 22 Dec 2014 11:02:00 GMT

←Older revision		Revision as of 11:02, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [http://www.ebi.ac.uk/pdbsum/1ahc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [http://www.ebi.ac.uk/pdbsum/1ahc PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
-	[[Category: Dong, Y.]]	+	[[Category: Dong, Y]]
-	[[Category: Ren, J.]]	+	[[Category: Ren, J]]
-	[[Category: Stuart, D I.]]	+	[[Category: Stuart, D I]]
-	[[Category: Wang, Y.]]	+	[[Category: Wang, Y]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]

OCA at 08:07, 30 July 2014

OCA — Wed, 30 Jul 2014 08:07:34 GMT

←Older revision		Revision as of 08:07, 30 July 2014
Line 1:		Line 1:
-	[[~~Image~~:1ahc.~~png\|left~~\|200px]]	+	==THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN==
		+	<StructureSection load='1ahc' size='340' side='right' caption='[[1ahc]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ahc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ahc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ahc RCSB], [http://www.ebi.ac.uk/pdbsum/1ahc PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/1ahc_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: alpha-Momorcharin (alpha MMC) is a type I ribosome-inactivating protein. It inhibits protein synthesis by hydrolytically removing a specific adenine residue from a highly conserved, single-stranded loop of rRNA. RESULTS: Here we describe the determination and refinement of the crystal structures of alpha MMC in the native state and in complexes with the product, adenine, and a substrate analogue, formycin 5'-monophosphate (FMP) at high resolution. Both adenine and the base of FMP are tightly bound; the ribose of bound FMP adopts a strained, high-energy conformation, which may mimic the structure of the transition state. CONCLUSIONS: These structures indicate that residues Tyr70, Glu160 and Arg163 of alpha MMC are the most critical for catalysis. We propose that the strained conformation of the ribose in the target adenosine weakens the glycoside bond. Partial protonation mediated by Arg163 then facilitates N-glycoside bond cleavage, leading to the formation of an oxycarbonium ion intermediate which is stabilized by the negatively-charged Glu160. Tyr70 adopts subtly different conformations in the three structures implying that it may be important in substrate recognition and perhaps catalysis.

-	~~{{STRUCTURE_1ahc\| PDB=1ahc \| SCENE= }}~~	+	The N-glycosidase mechanism of ribosome-inactivating proteins implied by crystal structures of alpha-momorcharin.,Ren J, Wang Y, Dong Y, Stuart DI Structure. 1994 Jan 15;2(1):7-16. PMID:8075985<ref>PMID:8075985</ref>

-	~~===THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8075985}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ahc]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Momordica_charantia Momordica charantia]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:008075985<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]

OCA: Protected "1ahc" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 11:44:17 GMT

Protected "1ahc" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:44, 21 October 2012

OCA at 11:44, 21 October 2012

OCA — Sun, 21 Oct 2012 11:44:13 GMT

←Older revision		Revision as of 11:44, 21 October 2012
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-	{{Seed}}
	[[Image:1ahc.png\|left\|200px]]		[[Image:1ahc.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1ahc\| PDB=1ahc \| SCENE= }}		{{STRUCTURE_1ahc\| PDB=1ahc \| SCENE= }}

	===THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN===		===THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN===

-
-	<!--
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	{{ABSTRACT_PUBMED_8075985}}		{{ABSTRACT_PUBMED_8075985}}

	==About this Structure==		==About this Structure==
-	~~1AHC~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA].	+	[[1ahc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8075985~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008075985</ref><references group="xtra"/>
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
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	[[Category: Wang, Y.]]		[[Category: Wang, Y.]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
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-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:51:11 2009''

OCA at 03:51, 18 February 2009

OCA — Wed, 18 Feb 2009 03:51:12 GMT

←Older revision		Revision as of 03:51, 18 February 2009
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	==About this Structure==		==About this Structure==
-	1AHC is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA].	+	1AHC is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHC OCA].

	==Reference==		==Reference==
-	~~The N-glycosidase mechanism of ribosome-inactivating proteins implied by crystal structures of alpha-momorcharin., Ren J, Wang Y, Dong Y, Stuart DI, Structure. 1994 Jan 15;2(1):7-16.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/8075985 8075985]~~	+	<ref group="xtra">PMID:8075985</ref><references group="xtra"/>
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
-	[[Category: Single protein]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Dong, Y.]]		[[Category: Dong, Y.]]
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	[[Category: Glycosidase]]		[[Category: Glycosidase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jun 30 16~~:51:~~09 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:51:11 2009''