1ai7 - Revision history

OCA at 06:30, 7 February 2024

OCA — Wed, 07 Feb 2024 06:30:47 GMT

←Older revision		Revision as of 06:30, 7 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ai7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AI7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ai7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AI7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=IPH:PHENOL'>IPH</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase]~~, ~~with EC number [https~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [https://pdbe.org/1ai7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ai7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ai7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [https://pdbe.org/1ai7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ai7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ai7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PAC_ECOLX PAC_ECOLX]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ai7 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ai7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The enzyme penicillin acylase (penicillin amidohydrolase EC 3.5.1. 11) catalyses the cleavage of the amide bond in the benzylpenicillin (penicillin G) side-chain to produce phenylacetic acid and 6-aminopenicillanic acid (6-APA). The enzyme is of great pharmaceutical importance, as the product 6-APA is the starting point for the synthesis of many semi-synthetic penicillin antibiotics. Studies have shown that the enzyme is specific for hydrolysis of phenylacetamide derivatives, but is more tolerant of features in the rest of the substrate. It is this property that has led to many other applications for the enzyme, and greater knowledge of the enzyme's structure and specificity could facilitate engineering of the enzyme, enhancing its potential for chemical and industrial applications. An extensive study of the binding of a series of phenylacetic acid derivatives has been carried out. A measure of the relative degree of inhibition of the enzyme by each of the compounds has been obtained using a competitive inhibition assay, and the structures of a number of these complexes have been determined by X-ray crystallography. The structures reveal a clear rationale for the observed kinetic results, but show also that some of the ligands cause a conformational change within the binding pocket. This change can generally be understood in terms of the size and orientation of the ligand within the active site.The results reveal that ligand binding in penicillin acylase is facilitated by certain amino acid residues that can adopt two distinct, energetically favourable positions in order to accommodate a variety of compounds within the active site. The structures of these complexes provide evidence for conformational changes in the substrate-binding region that may act as a switch in the mechanism of autocatalytic processing of this enzyme.
-
-	Ligand-induced conformational change in penicillin acylase.,Done SH, Brannigan JA, Moody PC, Hubbard RE J Mol Biol. 1998 Nov 27;284(2):463-75. PMID:9813130<ref>PMID:9813130</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ai7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Penicillin acylase\|Penicillin acylase]]		*[[Penicillin acylase\|Penicillin acylase]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Penicillin amidase]]~~	+	[[Category: Done SH]]
-	[[Category: Done~~, S H]]~~	+
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ligand induced conformational change~~]]	+

OCA at 12:18, 23 March 2022

OCA — Wed, 23 Mar 2022 12:18:02 GMT

←Older revision		Revision as of 12:18, 23 March 2022
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	<StructureSection load='1ai7' size='340' side='right'caption='[[1ai7]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1ai7' size='340' side='right'caption='[[1ai7]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ai7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1AI7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ai7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AI7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [~~http~~://pdbe.org/1ai7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ai7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ai7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [https://pdbe.org/1ai7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ai7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ai7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:58, 17 June 2020

OCA — Wed, 17 Jun 2020 10:58:28 GMT

←Older revision		Revision as of 10:58, 17 June 2020
Line 1:		Line 1:

	==PENICILLIN ACYLASE COMPLEXED WITH PHENOL==		==PENICILLIN ACYLASE COMPLEXED WITH PHENOL==
-	<StructureSection load='1ai7' size='340' side='right' caption='[[1ai7]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1ai7' size='340' side='right'caption='[[1ai7]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ai7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AI7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ai7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1AI7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [http://pdbe.org/1ai7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ai7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ai7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [http://pdbe.org/1ai7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ai7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ai7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
	[[Category: Penicillin amidase]]		[[Category: Penicillin amidase]]
	[[Category: Done, S H]]		[[Category: Done, S H]]

OCA at 08:55, 12 September 2018

OCA — Wed, 12 Sep 2018 08:55:25 GMT

←Older revision		Revision as of 08:55, 12 September 2018
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/1ai7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/1ai7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:31, 9 December 2016

OCA — Fri, 09 Dec 2016 20:31:37 GMT

←Older revision		Revision as of 20:31, 9 December 2016
Line 1:		Line 1:
		+
	==PENICILLIN ACYLASE COMPLEXED WITH PHENOL==		==PENICILLIN ACYLASE COMPLEXED WITH PHENOL==
	<StructureSection load='1ai7' size='340' side='right' caption='[[1ai7]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1ai7' size='340' side='right' caption='[[1ai7]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ai7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [http://pdbe.org/1ai7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ai7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ai7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ai7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ai7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 10:16, 22 December 2014

OCA — Mon, 22 Dec 2014 10:16:58 GMT

←Older revision		Revision as of 10:16, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ai7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AI7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ai7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AI7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ai7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ai7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Penicillin amidase]]		[[Category: Penicillin amidase]]
-	[[Category: Done, S H.]]	+	[[Category: Done, S H]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Ligand induced conformational change]]		[[Category: Ligand induced conformational change]]

OCA at 09:45, 13 August 2014

OCA — Wed, 13 Aug 2014 09:45:19 GMT

←Older revision		Revision as of 09:45, 13 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1ai7.~~png\|left~~\|200px]]	+	==PENICILLIN ACYLASE COMPLEXED WITH PHENOL==
		+	<StructureSection load='1ai7' size='340' side='right' caption='[[1ai7]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ai7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AI7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ai7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ai7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ai7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ai7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/1ai7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The enzyme penicillin acylase (penicillin amidohydrolase EC 3.5.1. 11) catalyses the cleavage of the amide bond in the benzylpenicillin (penicillin G) side-chain to produce phenylacetic acid and 6-aminopenicillanic acid (6-APA). The enzyme is of great pharmaceutical importance, as the product 6-APA is the starting point for the synthesis of many semi-synthetic penicillin antibiotics. Studies have shown that the enzyme is specific for hydrolysis of phenylacetamide derivatives, but is more tolerant of features in the rest of the substrate. It is this property that has led to many other applications for the enzyme, and greater knowledge of the enzyme's structure and specificity could facilitate engineering of the enzyme, enhancing its potential for chemical and industrial applications. An extensive study of the binding of a series of phenylacetic acid derivatives has been carried out. A measure of the relative degree of inhibition of the enzyme by each of the compounds has been obtained using a competitive inhibition assay, and the structures of a number of these complexes have been determined by X-ray crystallography. The structures reveal a clear rationale for the observed kinetic results, but show also that some of the ligands cause a conformational change within the binding pocket. This change can generally be understood in terms of the size and orientation of the ligand within the active site.The results reveal that ligand binding in penicillin acylase is facilitated by certain amino acid residues that can adopt two distinct, energetically favourable positions in order to accommodate a variety of compounds within the active site. The structures of these complexes provide evidence for conformational changes in the substrate-binding region that may act as a switch in the mechanism of autocatalytic processing of this enzyme.

-	~~{{STRUCTURE_1ai7\| PDB=1ai7 \| SCENE= }}~~	+	Ligand-induced conformational change in penicillin acylase.,Done SH, Brannigan JA, Moody PC, Hubbard RE J Mol Biol. 1998 Nov 27;284(2):463-75. PMID:9813130<ref>PMID:9813130</ref>

-	~~===PENICILLIN ACYLASE COMPLEXED WITH PHENOL===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	==~~About this Structure~~==	+	*[[Penicillin acylase\|Penicillin acylase]]
-	[[~~1ai7~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA].	+	== References ==
-		+	<references/>
-	==~~Reference~~==	+	__TOC__
-	<~~ref group="xtra">PMID:009813130<~~/~~ref~~><~~references group="xtra"~~/>	+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Penicillin amidase]]		[[Category: Penicillin amidase]]

OCA at 14:07, 8 November 2012

OCA — Thu, 08 Nov 2012 14:07:08 GMT

←Older revision		Revision as of 14:07, 8 November 2012
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	===PENICILLIN ACYLASE COMPLEXED WITH PHENOL===		===PENICILLIN ACYLASE COMPLEXED WITH PHENOL===

-	{{ABSTRACT_PUBMED_9813130}}

	==About this Structure==		==About this Structure==

OCA: Protected "1ai7" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 14:27:45 GMT

Protected "1ai7" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:27, 21 October 2012

OCA at 14:27, 21 October 2012

OCA — Sun, 21 Oct 2012 14:27:42 GMT

←Older revision		Revision as of 14:27, 21 October 2012
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-	{{Seed}}
	[[Image:1ai7.png\|left\|200px]]		[[Image:1ai7.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ai7", creates the "Structure Box" on the page.
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1ai7\| PDB=1ai7 \| SCENE= }}		{{STRUCTURE_1ai7\| PDB=1ai7 \| SCENE= }}

	===PENICILLIN ACYLASE COMPLEXED WITH PHENOL===		===PENICILLIN ACYLASE COMPLEXED WITH PHENOL===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9813130}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_9813130}}		{{ABSTRACT_PUBMED_9813130}}

	==About this Structure==		==About this Structure==
-	~~1AI7~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA].	+	[[1ai7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AI7 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9813130~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009813130</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Penicillin amidase]]		[[Category: Penicillin amidase]]
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Ligand induced conformational change]]		[[Category: Ligand induced conformational change]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:19:05 2009''