1akt - Revision history

OCA at 06:31, 7 February 2024

OCA — Wed, 07 Feb 2024 06:31:30 GMT

←Older revision		Revision as of 06:31, 7 February 2024
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	<StructureSection load='1akt' size='340' side='right'caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1akt' size='340' side='right'caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Desvh Desvh~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [https://pdbe.org/1akt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [https://www.ebi.ac.uk/pdbsum/1akt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [https://pdbe.org/1akt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [https://www.ebi.ac.uk/pdbsum/1akt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FLAV_DESVH FLAV_DESVH]] Low-potential electron donor to a number of redox enzymes.	+	[https://www.uniprot.org/uniprot/FLAV_DESVH FLAV_DESVH] Low-potential electron donor to a number of redox enzymes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1akt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1akt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Mutants of the electron-transfer protein flavodoxin from Desulfovibrio vulgaris were made by site-directed mutagenesis to investigate the role of glycine-61 in stabilizing the semiquinone of FMN by the protein and in controlling the flavin redox potentials. The spectroscopic properties, oxidation-reduction potentials, and flavin-binding properties of the mutant proteins, G61A/N/V and L, were compared with those of wild-type flavodoxin. The affinities of all of the mutant apoproteins for FMN and riboflavin were less than that of the wild-type apoprotein, and the redox potentials of the two 1-electron steps in the reduction of the complex with FMN were also affected by the mutations. Values for the dissociation constants of the complexes of the apoprotein with the semiquinone and hydroquinone forms of FMN were calculated from the redox potentials and the dissociation constant of the oxidized complex and used to derive the free energies of binding of the FMN in its three oxidation states. These showed that the semiquinone is destabilized in all of the mutants, and that the extent of destabilization tends to increase with increasing bulkiness of the side chain at residue 61. It is concluded that the hydrogen bond between the carbonyl of glycine-61 and N(5)H of FMN semiquinone in wild-type flavodoxin is either absent or severely impaired in the mutants. X-ray crystal structure analysis of the oxidized forms of the four mutant proteins shows that the protein loop that contains residue 61 is moved away from the flavin by 5-6 A. The hydrogen bond formed between the backbone nitrogen of aspartate-62 and O(4) of the dimethylisoalloxazine of the flavin in wild-type flavodoxin is absent in the mutants. Reliable structural information was not obtained for the reduced forms of the mutant proteins, but if the mutants change conformation when the flavin is reduced to the semiquinone, to facilitate hydrogen bonding between N(5)H and the carbonyl of residue 61, then the change must be different from that known to occur in wild-type flavodoxin.
-
-	Modulation of the redox potentials of FMN in Desulfovibrio vulgaris flavodoxin: thermodynamic properties and crystal structures of glycine-61 mutants.,O'Farrell PA, Walsh MA, McCarthy AA, Higgins TM, Voordouw G, Mayhew SG Biochemistry. 1998 Jun 9;37(23):8405-16. PMID:9622492<ref>PMID:9622492</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1akt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Flavodoxin 3D structures\|Flavodoxin 3D structures]]		*[[Flavodoxin 3D structures\|Flavodoxin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Desvh~~]]	+	[[Category: Desulfovibrio vulgaris str. Hildenborough]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Higgins, T]]	+	[[Category: Higgins T]]
-	[[Category: Mccarthy, A]]	+	[[Category: Mccarthy A]]
-	[[Category: Walsh, M]]	+	[[Category: Walsh M]]
-	~~[[Category: Electron transfer]]~~	+
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Flavodoxin]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Fmn]]~~	+
-	~~[[Category: Mutant~~]]	+

OCA at 14:39, 3 November 2021

OCA — Wed, 03 Nov 2021 14:39:44 GMT

←Older revision		Revision as of 14:39, 3 November 2021
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	<StructureSection load='1akt' size='340' side='right'caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1akt' size='340' side='right'caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desvh Desvh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [https://pdbe.org/1akt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [https://www.ebi.ac.uk/pdbsum/1akt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [https://pdbe.org/1akt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [https://www.ebi.ac.uk/pdbsum/1akt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akt ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Desvh]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Higgins, T]]		[[Category: Higgins, T]]

OCA at 10:21, 17 February 2021

OCA — Wed, 17 Feb 2021 10:21:27 GMT

←Older revision		Revision as of 10:21, 17 February 2021
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	<StructureSection load='1akt' size='340' side='right'caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1akt' size='340' side='right'caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AKT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [~~http~~://pdbe.org/1akt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1akt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1akt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [https://pdbe.org/1akt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [https://www.ebi.ac.uk/pdbsum/1akt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FLAV_DESVH FLAV_DESVH]] Low-potential electron donor to a number of redox enzymes.	+	[[https://www.uniprot.org/uniprot/FLAV_DESVH FLAV_DESVH]] Low-potential electron donor to a number of redox enzymes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Flavodoxin\|Flavodoxin]]	+	*[[Flavodoxin 3D structures\|Flavodoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:07, 10 May 2019

OCA — Fri, 10 May 2019 14:07:16 GMT

←Older revision		Revision as of 14:07, 10 May 2019
Line 1:		Line 1:

	==G61N OXIDIZED FLAVODOXIN MUTANT==		==G61N OXIDIZED FLAVODOXIN MUTANT==
-	<StructureSection load='1akt' size='340' side='right' caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1akt' size='340' side='right'caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AKT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AKT FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ak/1akt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ak/1akt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Higgins, T]]		[[Category: Higgins, T]]
	[[Category: Mccarthy, A]]		[[Category: Mccarthy, A]]

OCA at 09:15, 9 August 2017

OCA — Wed, 09 Aug 2017 09:15:24 GMT

←Older revision		Revision as of 09:15, 9 August 2017
Line 1:		Line 1:
		+
	==G61N OXIDIZED FLAVODOXIN MUTANT==		==G61N OXIDIZED FLAVODOXIN MUTANT==
	<StructureSection load='1akt' size='340' side='right' caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1akt' size='340' side='right' caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Desvh Desvh]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AKT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AKT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [http://pdbe.org/1akt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [http://www.ebi.ac.uk/pdbsum/1akt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [http://pdbe.org/1akt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [http://www.ebi.ac.uk/pdbsum/1akt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1akt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1akt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Desvh]]
	[[Category: Higgins, T]]		[[Category: Higgins, T]]
	[[Category: Mccarthy, A]]		[[Category: Mccarthy, A]]

OCA at 23:41, 11 September 2015

OCA — Fri, 11 Sep 2015 23:41:07 GMT

←Older revision		Revision as of 23:41, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1akt' size='340' side='right' caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1akt' size='340' side='right' caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Desulfovibrio_vulgaris_str._hildenborough Desulfovibrio vulgaris str. hildenborough~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AKT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desvh Desvh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AKT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [http://www.ebi.ac.uk/pdbsum/1akt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [http://pdbe.org/1akt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [http://www.ebi.ac.uk/pdbsum/1akt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1akt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Desulfovibrio vulgaris str. hildenborough~~]]	+	[[Category: Desvh]]
	[[Category: Higgins, T]]		[[Category: Higgins, T]]
	[[Category: Mccarthy, A]]		[[Category: Mccarthy, A]]

OCA at 20:57, 25 December 2014

OCA — Thu, 25 Dec 2014 20:57:06 GMT

←Older revision		Revision as of 20:57, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [http://www.ebi.ac.uk/pdbsum/1akt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [http://www.ebi.ac.uk/pdbsum/1akt PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FLAV_DESVH FLAV_DESVH]] Low-potential electron donor to a number of redox enzymes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:35, 22 December 2014

OCA — Mon, 22 Dec 2014 08:35:08 GMT

←Older revision		Revision as of 08:35, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._hildenborough Desulfovibrio vulgaris str. hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AKT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._hildenborough Desulfovibrio vulgaris str. hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AKT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [http://www.ebi.ac.uk/pdbsum/1akt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [http://www.ebi.ac.uk/pdbsum/1akt PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Desulfovibrio vulgaris str. hildenborough]]		[[Category: Desulfovibrio vulgaris str. hildenborough]]
-	[[Category: Higgins, T.]]	+	[[Category: Higgins, T]]
-	[[Category: Mccarthy, A.]]	+	[[Category: Mccarthy, A]]
-	[[Category: Walsh, M.]]	+	[[Category: Walsh, M]]
	[[Category: Electron transfer]]		[[Category: Electron transfer]]
	[[Category: Electron transport]]		[[Category: Electron transport]]

OCA at 08:09, 30 July 2014

OCA — Wed, 30 Jul 2014 08:09:15 GMT

←Older revision		Revision as of 08:09, 30 July 2014
Line 1:		Line 1:
-	[[~~Image~~:1akt.~~png\|left~~\|200px]]	+	==G61N OXIDIZED FLAVODOXIN MUTANT==
		+	<StructureSection load='1akt' size='340' side='right' caption='[[1akt]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._hildenborough Desulfovibrio vulgaris str. hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AKT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [http://www.ebi.ac.uk/pdbsum/1akt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ak/1akt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mutants of the electron-transfer protein flavodoxin from Desulfovibrio vulgaris were made by site-directed mutagenesis to investigate the role of glycine-61 in stabilizing the semiquinone of FMN by the protein and in controlling the flavin redox potentials. The spectroscopic properties, oxidation-reduction potentials, and flavin-binding properties of the mutant proteins, G61A/N/V and L, were compared with those of wild-type flavodoxin. The affinities of all of the mutant apoproteins for FMN and riboflavin were less than that of the wild-type apoprotein, and the redox potentials of the two 1-electron steps in the reduction of the complex with FMN were also affected by the mutations. Values for the dissociation constants of the complexes of the apoprotein with the semiquinone and hydroquinone forms of FMN were calculated from the redox potentials and the dissociation constant of the oxidized complex and used to derive the free energies of binding of the FMN in its three oxidation states. These showed that the semiquinone is destabilized in all of the mutants, and that the extent of destabilization tends to increase with increasing bulkiness of the side chain at residue 61. It is concluded that the hydrogen bond between the carbonyl of glycine-61 and N(5)H of FMN semiquinone in wild-type flavodoxin is either absent or severely impaired in the mutants. X-ray crystal structure analysis of the oxidized forms of the four mutant proteins shows that the protein loop that contains residue 61 is moved away from the flavin by 5-6 A. The hydrogen bond formed between the backbone nitrogen of aspartate-62 and O(4) of the dimethylisoalloxazine of the flavin in wild-type flavodoxin is absent in the mutants. Reliable structural information was not obtained for the reduced forms of the mutant proteins, but if the mutants change conformation when the flavin is reduced to the semiquinone, to facilitate hydrogen bonding between N(5)H and the carbonyl of residue 61, then the change must be different from that known to occur in wild-type flavodoxin.

-	~~{{STRUCTURE_1akt\| PDB=1akt \| SCENE= }}~~	+	Modulation of the redox potentials of FMN in Desulfovibrio vulgaris flavodoxin: thermodynamic properties and crystal structures of glycine-61 mutants.,O'Farrell PA, Walsh MA, McCarthy AA, Higgins TM, Voordouw G, Mayhew SG Biochemistry. 1998 Jun 9;37(23):8405-16. PMID:9622492<ref>PMID:9622492</ref>

-	~~===G61N OXIDIZED FLAVODOXIN MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9622492}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1akt]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Desulfovibrio_vulgaris_str~~.~~_hildenborough Desulfovibrio vulgaris str. hildenborough]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA].~~	+

	==See Also==		==See Also==
	*[[Flavodoxin\|Flavodoxin]]		*[[Flavodoxin\|Flavodoxin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009622492<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Desulfovibrio vulgaris str. hildenborough]]		[[Category: Desulfovibrio vulgaris str. hildenborough]]
	[[Category: Higgins, T.]]		[[Category: Higgins, T.]]

OCA at 21:33, 20 October 2012

OCA — Sat, 20 Oct 2012 21:33:08 GMT

←Older revision		Revision as of 21:33, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1akt]] is a 1 chain structure ~~of [[Flavodoxin]]~~ with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._hildenborough Desulfovibrio vulgaris str. hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA].	+	[[1akt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._hildenborough Desulfovibrio vulgaris str. hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA].

	==See Also==		==See Also==