1aoz - Revision history

OCA at 15:26, 13 March 2024

OCA — Wed, 13 Mar 2024 15:26:46 GMT

←Older revision		Revision as of 15:26, 13 March 2024
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	<StructureSection load='1aoz' size='340' side='right'caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1aoz' size='340' side='right'caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cucpm Cucpm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cucurbita_pepo_var._melopepo Cucurbita pepo var. melopepo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [https://pdbe.org/1aoz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [https://www.ebi.ac.uk/pdbsum/1aoz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aoz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [https://pdbe.org/1aoz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [https://www.ebi.ac.uk/pdbsum/1aoz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aoz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ASO_CUCPM ASO_CUCPM]] May be involved in a redox system involving ascorbic acid.	+	[https://www.uniprot.org/uniprot/ASO_CUCPM ASO_CUCPM] May be involved in a redox system involving ascorbic acid.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aoz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aoz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of the fully oxidized form of ascorbate oxidase (EC 1.10.3.3) from Zucchini has been refined at 1.90 A (1 A = 0.1 nm) resolution, using an energy-restrained least-squares refinement procedure. The refined model, which includes 8764 protein atoms, 9 copper atoms and 970 solvent molecules, has a crystallographic R-factor of 20.3% for 85,252 reflections between 8 and 1.90 A resolution. The root-mean-square deviation in bond lengths and bond angles from ideal values is 0.011 A and 2.99 degrees, respectively. The subunits of 552 residues (70,000 Mr) are arranged as tetramers with D2 symmetry. One of the dyads is realized by the crystallographic axis parallel to the c-axis giving one dimer in the asymmetric unit. The dimer related about this crystallographic axis is suggested as the dimer present in solution. Asn92 is the attachment site for one of the two N-linked sugar moieties, which has defined electron density for the N-linked N-acetyl-glucosamine ring. Each subunit is built up by three domains arranged sequentially on the polypeptide chain and tightly associated in space. The folding of all three domains is of a similar beta-barrel type and related to plastocyanin and azurin. An analysis of intra- and intertetramer hydrogen bond and van der Waals interactions is presented. Each subunit has four copper atoms bound as mononuclear and trinuclear species. The mononuclear copper has two histidine, a cysteine and a methionine ligand and represents the type-1 copper. It is located in domain 3. The bond lengths of the type-1 copper centre are comparable to the values for oxidized plastocyanin. The trinuclear cluster has eight histidine ligands symmetrically supplied from domain 1 and 3. It may be subdivided into a pair of copper atoms with histidine ligands whose ligating N-atoms (5 NE2 atoms and one ND1 atom) are arranged trigonal prismatic. The pair is the putative type-3 copper. The remaining copper has two histidine ligands and is the putative spectroscopic type-2 copper. Two oxygen atoms are bound to the trinuclear species as OH- or O2- and bridging the putative type-3 copper pair and as OH- or H2O bound to the putative type-2 copper trans to the copper pair. The bond lengths within the trinuclear copper site are similar to comparable binuclear model compounds. The putative binding site for the reducing substrate is close to the type-1 copper.(ABSTRACT TRUNCATED AT 400 WORDS)
-
-	Refined crystal structure of ascorbate oxidase at 1.9 A resolution.,Messerschmidt A, Ladenstein R, Huber R, Bolognesi M, Avigliano L, Petruzzelli R, Rossi A, Finazzi-Agro A J Mol Biol. 1992 Mar 5;224(1):179-205. PMID:1548698<ref>PMID:1548698</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1aoz" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cucpm]]~~	+	[[Category: Cucurbita pepo var. melopepo]]
-	~~[[Category: L-ascorbate oxidase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Huber, R]]	+	[[Category: Huber R]]
-	[[Category: Ladenstein, R]]	+	[[Category: Ladenstein R]]
-	[[Category: Messerschmidt, A]]	+	[[Category: Messerschmidt A]]

OCA at 10:38, 19 May 2021

OCA — Wed, 19 May 2021 10:38:06 GMT

←Older revision		Revision as of 10:38, 19 May 2021
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	<StructureSection load='1aoz' size='340' side='right'caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1aoz' size='340' side='right'caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cucpm Cucpm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cucpm Cucpm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [~~http~~://pdbe.org/1aoz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1aoz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1aoz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [https://pdbe.org/1aoz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [https://www.ebi.ac.uk/pdbsum/1aoz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aoz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ASO_CUCPM ASO_CUCPM]] May be involved in a redox system involving ascorbic acid.	+	[[https://www.uniprot.org/uniprot/ASO_CUCPM ASO_CUCPM]] May be involved in a redox system involving ascorbic acid.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:06, 14 August 2019

OCA — Wed, 14 Aug 2019 18:06:49 GMT

←Older revision		Revision as of 18:06, 14 August 2019
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	==REFINED CRYSTAL STRUCTURE OF ASCORBATE OXIDASE AT 1.9 ANGSTROMS RESOLUTION==		==REFINED CRYSTAL STRUCTURE OF ASCORBATE OXIDASE AT 1.9 ANGSTROMS RESOLUTION==
-	<StructureSection load='1aoz' size='340' side='right' caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1aoz' size='340' side='right'caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucpm Cucpm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucpm Cucpm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ao/1aoz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ao/1aoz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	[[Category: Cucpm]]		[[Category: Cucpm]]
	[[Category: L-ascorbate oxidase]]		[[Category: L-ascorbate oxidase]]
		+	[[Category: Large Structures]]
	[[Category: Huber, R]]		[[Category: Huber, R]]
	[[Category: Ladenstein, R]]		[[Category: Ladenstein, R]]
	[[Category: Messerschmidt, A]]		[[Category: Messerschmidt, A]]

OCA at 10:46, 8 November 2017

OCA — Wed, 08 Nov 2017 10:46:39 GMT

←Older revision		Revision as of 10:46, 8 November 2017
Line 1:		Line 1:
		+
	==REFINED CRYSTAL STRUCTURE OF ASCORBATE OXIDASE AT 1.9 ANGSTROMS RESOLUTION==		==REFINED CRYSTAL STRUCTURE OF ASCORBATE OXIDASE AT 1.9 ANGSTROMS RESOLUTION==
	<StructureSection load='1aoz' size='340' side='right' caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1aoz' size='340' side='right' caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [http://pdbe.org/1aoz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [http://www.ebi.ac.uk/pdbsum/1aoz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [http://pdbe.org/1aoz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [http://www.ebi.ac.uk/pdbsum/1aoz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1aoz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 19:16, 7 February 2016

OCA — Sun, 07 Feb 2016 19:16:09 GMT

←Older revision		Revision as of 19:16, 7 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aoz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:17, 10 September 2015

OCA — Thu, 10 Sep 2015 04:17:56 GMT

←Older revision		Revision as of 04:17, 10 September 2015
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	<StructureSection load='1aoz' size='340' side='right' caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1aoz' size='340' side='right' caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cucurbita_pepo_var._melopepo Cucurbita pepo var. melopepo~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucpm Cucpm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [http://www.ebi.ac.uk/pdbsum/1aoz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [http://pdbe.org/1aoz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [http://www.ebi.ac.uk/pdbsum/1aoz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1aoz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cucurbita pepo var. melopepo~~]]	+	[[Category: Cucpm]]
	[[Category: L-ascorbate oxidase]]		[[Category: L-ascorbate oxidase]]
	[[Category: Huber, R]]		[[Category: Huber, R]]
	[[Category: Ladenstein, R]]		[[Category: Ladenstein, R]]
	[[Category: Messerschmidt, A]]		[[Category: Messerschmidt, A]]

OCA at 17:39, 24 December 2014

OCA — Wed, 24 Dec 2014 17:39:49 GMT

←Older revision		Revision as of 17:39, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [http://www.ebi.ac.uk/pdbsum/1aoz PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [http://www.ebi.ac.uk/pdbsum/1aoz PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ASO_CUCPM ASO_CUCPM]] May be involved in a redox system involving ascorbic acid.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:05, 22 December 2014

OCA — Mon, 22 Dec 2014 09:05:29 GMT

←Older revision		Revision as of 09:05, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucurbita_pepo_var._melopepo Cucurbita pepo var. melopepo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucurbita_pepo_var._melopepo Cucurbita pepo var. melopepo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [http://www.ebi.ac.uk/pdbsum/1aoz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [http://www.ebi.ac.uk/pdbsum/1aoz PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	[[Category: Cucurbita pepo var. melopepo]]		[[Category: Cucurbita pepo var. melopepo]]
	[[Category: L-ascorbate oxidase]]		[[Category: L-ascorbate oxidase]]
-	[[Category: Huber, R.]]	+	[[Category: Huber, R]]
-	[[Category: Ladenstein, R.]]	+	[[Category: Ladenstein, R]]
-	[[Category: Messerschmidt, A.]]	+	[[Category: Messerschmidt, A]]

OCA at 08:08, 30 July 2014

OCA — Wed, 30 Jul 2014 08:08:53 GMT

←Older revision		Revision as of 08:08, 30 July 2014
Line 1:		Line 1:
-	[[~~Image~~:1aoz.~~png\|left~~\|200px]]	+	==REFINED CRYSTAL STRUCTURE OF ASCORBATE OXIDASE AT 1.9 ANGSTROMS RESOLUTION==
		+	<StructureSection load='1aoz' size='340' side='right' caption='[[1aoz]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1aoz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucurbita_pepo_var._melopepo Cucurbita pepo var. melopepo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AOZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_oxidase L-ascorbate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.3 1.10.3.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aoz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1aoz RCSB], [http://www.ebi.ac.uk/pdbsum/1aoz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ao/1aoz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the fully oxidized form of ascorbate oxidase (EC 1.10.3.3) from Zucchini has been refined at 1.90 A (1 A = 0.1 nm) resolution, using an energy-restrained least-squares refinement procedure. The refined model, which includes 8764 protein atoms, 9 copper atoms and 970 solvent molecules, has a crystallographic R-factor of 20.3% for 85,252 reflections between 8 and 1.90 A resolution. The root-mean-square deviation in bond lengths and bond angles from ideal values is 0.011 A and 2.99 degrees, respectively. The subunits of 552 residues (70,000 Mr) are arranged as tetramers with D2 symmetry. One of the dyads is realized by the crystallographic axis parallel to the c-axis giving one dimer in the asymmetric unit. The dimer related about this crystallographic axis is suggested as the dimer present in solution. Asn92 is the attachment site for one of the two N-linked sugar moieties, which has defined electron density for the N-linked N-acetyl-glucosamine ring. Each subunit is built up by three domains arranged sequentially on the polypeptide chain and tightly associated in space. The folding of all three domains is of a similar beta-barrel type and related to plastocyanin and azurin. An analysis of intra- and intertetramer hydrogen bond and van der Waals interactions is presented. Each subunit has four copper atoms bound as mononuclear and trinuclear species. The mononuclear copper has two histidine, a cysteine and a methionine ligand and represents the type-1 copper. It is located in domain 3. The bond lengths of the type-1 copper centre are comparable to the values for oxidized plastocyanin. The trinuclear cluster has eight histidine ligands symmetrically supplied from domain 1 and 3. It may be subdivided into a pair of copper atoms with histidine ligands whose ligating N-atoms (5 NE2 atoms and one ND1 atom) are arranged trigonal prismatic. The pair is the putative type-3 copper. The remaining copper has two histidine ligands and is the putative spectroscopic type-2 copper. Two oxygen atoms are bound to the trinuclear species as OH- or O2- and bridging the putative type-3 copper pair and as OH- or H2O bound to the putative type-2 copper trans to the copper pair. The bond lengths within the trinuclear copper site are similar to comparable binuclear model compounds. The putative binding site for the reducing substrate is close to the type-1 copper.(ABSTRACT TRUNCATED AT 400 WORDS)

-	~~{{STRUCTURE_1aoz\| PDB=1aoz \| SCENE= }}~~	+	Refined crystal structure of ascorbate oxidase at 1.9 A resolution.,Messerschmidt A, Ladenstein R, Huber R, Bolognesi M, Avigliano L, Petruzzelli R, Rossi A, Finazzi-Agro A J Mol Biol. 1992 Mar 5;224(1):179-205. PMID:1548698<ref>PMID:1548698</ref>

-	~~===REFINED CRYSTAL STRUCTURE OF ASCORBATE OXIDASE AT 1.9 ANGSTROMS RESOLUTION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_1548698}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1aoz]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Cucurbita_pepo_var~~.~~_melopepo Cucurbita pepo var. melopepo]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1AOZ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:001548698<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Cucurbita pepo var. melopepo]]		[[Category: Cucurbita pepo var. melopepo]]
	[[Category: L-ascorbate oxidase]]		[[Category: L-ascorbate oxidase]]

OCA: Protected "1aoz" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 11:47:15 GMT

Protected "1aoz" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:47, 21 October 2012