1auw - Revision history

OCA at 06:33, 7 February 2024

OCA — Wed, 07 Feb 2024 06:33:33 GMT

←Older revision		Revision as of 06:33, 7 February 2024
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	<StructureSection load='1auw' size='340' side='right'caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1auw' size='340' side='right'caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Anapl Anapl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AUW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AUW FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~https://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.~~2.~~1 4.3.2.1] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [https://pdbe.org/1auw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [https://www.ebi.ac.uk/pdbsum/1auw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1auw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [https://pdbe.org/1auw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [https://www.ebi.ac.uk/pdbsum/1auw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1auw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>	+	[https://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1auw ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1auw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The major soluble protein component of avian and reptilian eye lenses, delta crystallin, is highly homologous to the urea cycle enzyme, argininosuccinate lyase (ASL). In duck lenses there are two highly homologous delta crystallins, termed delta I and delta II, that are 94% identical in amino acid sequence. While delta II crystallin has been shown to exhibit ASL activity in vitro, delta I crystallin is inactive. The X-ray structure of a His to Asn mutant of duck delta II crystallin (H91N) has been determined to 2.5 A resolution using the molecular replacement technique. The overall fold of the protein is similar to other members of the superfamily to which this protein belongs, with the active site located in a cleft between three different monomers of the tetrameric protein. A reexamination of the kinetic properties of the H91N mutant reveals that the mutant has 10% wild-type activity. The Vmax of the mutant protein is identical to that of the wild-type protein, but a 10-fold increase in the Michaelis constant is seen, suggesting that His 91 is involved in binding the substrate. In an effort to determine the reasons for the loss of enzymatic activity in delta I crystallin, a structural comparison of the H91N mutant with the enzymatically inactive turkey delta I crystallin has been performed. This study revealed a remarkable similarity in the overall structures of the two proteins. Three regions of secondary structure do differ significantly between the two models; these include the N-terminal tail, a loop containing residues 76-91, and a cis versus trans peptide linkage at residue Thr 322. The cis to trans peptide variation appears to be an interspecies difference between turkey and duck and is therefore not directly involved in the loss of enzymatic activity. All the residues implicated in the catalytic mechanism are conserved in both the active and inactive proteins, and given the linearity of the relationship between the enzymatic activity of duck delta I/delta II heterotetramers and their delta II content (Piatigorsky & Horwitz, 1996), it is evident from the structure that only one of the three domains that contributes to the active site is responsible for the loss of activity in the delta I protein. Given the structural differences found in domain 1 (N-terminal tail and 76-91 loop), we postulate that these differences are responsible for the loss of catalytic activity in the delta I crystallin protein and that the delta I protein is inactive because it no longer binds the substrate.
-
-	Structural comparison of the enzymatically active and inactive forms of delta crystallin and the role of histidine 91.,Abu-Abed M, Turner MA, Vallee F, Simpson A, Slingsby C, Howell PL Biochemistry. 1997 Nov 18;36(46):14012-22. PMID:9369472<ref>PMID:9369472</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1auw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Anapl]]~~	+	[[Category: Anas platyrhynchos]]
-	~~[[Category: Argininosuccinate lyase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abu-Abed, M]]	+	[[Category: Abu-Abed M]]
-	[[Category: Howell~~, P L~~]]	+	[[Category: Howell PL]]
-	[[Category: Vallee, F]]	+	[[Category: Vallee F]]
-	~~[[Category: Arginosuccinate lyase]]~~	+
-	~~[[Category: Delta 2 crystallin]]~~	+
-	~~[[Category: Eye lens protein~~]]	+

OCA at 10:38, 19 May 2021

OCA — Wed, 19 May 2021 10:38:35 GMT

←Older revision		Revision as of 10:38, 19 May 2021
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	<StructureSection load='1auw' size='340' side='right'caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1auw' size='340' side='right'caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AUW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AUW FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [~~http~~://pdbe.org/1auw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1auw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1auw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [https://pdbe.org/1auw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [https://www.ebi.ac.uk/pdbsum/1auw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1auw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>	+	[[https://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 17:56, 14 August 2019

OCA — Wed, 14 Aug 2019 17:56:34 GMT

←Older revision		Revision as of 17:56, 14 August 2019
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	==H91N DELTA 2 CRYSTALLIN FROM DUCK==		==H91N DELTA 2 CRYSTALLIN FROM DUCK==
-	<StructureSection load='1auw' size='340' side='right' caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1auw' size='340' side='right'caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AUW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AUW FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/1auw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/1auw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1auw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1auw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Crystallin 3D structures\|Crystallin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	[[Category: Anapl]]		[[Category: Anapl]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
		+	[[Category: Large Structures]]
	[[Category: Abu-Abed, M]]		[[Category: Abu-Abed, M]]
	[[Category: Howell, P L]]		[[Category: Howell, P L]]

OCA at 10:55, 8 November 2017

OCA — Wed, 08 Nov 2017 10:55:44 GMT

←Older revision		Revision as of 10:55, 8 November 2017
Line 1:		Line 1:
		+
	==H91N DELTA 2 CRYSTALLIN FROM DUCK==		==H91N DELTA 2 CRYSTALLIN FROM DUCK==
	<StructureSection load='1auw' size='340' side='right' caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1auw' size='340' side='right' caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AUW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AUW FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [http://pdbe.org/1auw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [http://www.ebi.ac.uk/pdbsum/1auw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [http://pdbe.org/1auw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [http://www.ebi.ac.uk/pdbsum/1auw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1auw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1auw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1auw" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Crystalline\|Crystalline]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:53, 7 February 2016

OCA — Sun, 07 Feb 2016 14:53:25 GMT

←Older revision		Revision as of 14:53, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1auw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:33, 10 September 2015

OCA — Thu, 10 Sep 2015 02:33:19 GMT

←Older revision		Revision as of 02:33, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1auw' size='340' side='right' caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1auw' size='340' side='right' caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Anas_platyrhynchos Anas platyrhynchos~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AUW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AUW FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [http://www.ebi.ac.uk/pdbsum/1auw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [http://pdbe.org/1auw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [http://www.ebi.ac.uk/pdbsum/1auw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1auw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Anas platyrhynchos~~]]	+	[[Category: Anapl]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
	[[Category: Abu-Abed, M]]		[[Category: Abu-Abed, M]]

OCA at 10:50, 22 December 2014

OCA — Mon, 22 Dec 2014 10:50:01 GMT

←Older revision		Revision as of 10:50, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AUW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AUW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [http://www.ebi.ac.uk/pdbsum/1auw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [http://www.ebi.ac.uk/pdbsum/1auw PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
-	[[Category: Abu-Abed, M.]]	+	[[Category: Abu-Abed, M]]
-	[[Category: Howell, P L.]]	+	[[Category: Howell, P L]]
-	[[Category: Vallee, F.]]	+	[[Category: Vallee, F]]
	[[Category: Arginosuccinate lyase]]		[[Category: Arginosuccinate lyase]]
	[[Category: Delta 2 crystallin]]		[[Category: Delta 2 crystallin]]
	[[Category: Eye lens protein]]		[[Category: Eye lens protein]]

OCA at 08:15, 30 July 2014

OCA — Wed, 30 Jul 2014 08:15:05 GMT

←Older revision		Revision as of 08:15, 30 July 2014
Line 1:		Line 1:
-	[[~~Image~~:1auw.~~png\|left~~\|200px]]	+	==H91N DELTA 2 CRYSTALLIN FROM DUCK==
		+	<StructureSection load='1auw' size='340' side='right' caption='[[1auw]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AUW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1auw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1auw RCSB], [http://www.ebi.ac.uk/pdbsum/1auw PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/1auw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The major soluble protein component of avian and reptilian eye lenses, delta crystallin, is highly homologous to the urea cycle enzyme, argininosuccinate lyase (ASL). In duck lenses there are two highly homologous delta crystallins, termed delta I and delta II, that are 94% identical in amino acid sequence. While delta II crystallin has been shown to exhibit ASL activity in vitro, delta I crystallin is inactive. The X-ray structure of a His to Asn mutant of duck delta II crystallin (H91N) has been determined to 2.5 A resolution using the molecular replacement technique. The overall fold of the protein is similar to other members of the superfamily to which this protein belongs, with the active site located in a cleft between three different monomers of the tetrameric protein. A reexamination of the kinetic properties of the H91N mutant reveals that the mutant has 10% wild-type activity. The Vmax of the mutant protein is identical to that of the wild-type protein, but a 10-fold increase in the Michaelis constant is seen, suggesting that His 91 is involved in binding the substrate. In an effort to determine the reasons for the loss of enzymatic activity in delta I crystallin, a structural comparison of the H91N mutant with the enzymatically inactive turkey delta I crystallin has been performed. This study revealed a remarkable similarity in the overall structures of the two proteins. Three regions of secondary structure do differ significantly between the two models; these include the N-terminal tail, a loop containing residues 76-91, and a cis versus trans peptide linkage at residue Thr 322. The cis to trans peptide variation appears to be an interspecies difference between turkey and duck and is therefore not directly involved in the loss of enzymatic activity. All the residues implicated in the catalytic mechanism are conserved in both the active and inactive proteins, and given the linearity of the relationship between the enzymatic activity of duck delta I/delta II heterotetramers and their delta II content (Piatigorsky & Horwitz, 1996), it is evident from the structure that only one of the three domains that contributes to the active site is responsible for the loss of activity in the delta I protein. Given the structural differences found in domain 1 (N-terminal tail and 76-91 loop), we postulate that these differences are responsible for the loss of catalytic activity in the delta I crystallin protein and that the delta I protein is inactive because it no longer binds the substrate.

-	~~{{STRUCTURE_1auw\| PDB=1auw \| SCENE= }}~~	+	Structural comparison of the enzymatically active and inactive forms of delta crystallin and the role of histidine 91.,Abu-Abed M, Turner MA, Vallee F, Simpson A, Slingsby C, Howell PL Biochemistry. 1997 Nov 18;36(46):14012-22. PMID:9369472<ref>PMID:9369472</ref>

-	~~===H91N DELTA 2 CRYSTALLIN FROM DUCK===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9369472}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1auw]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Anas_platyrhynchos Anas platyrhynchos]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA].~~	+

	==See Also==		==See Also==
	*[[Crystalline\|Crystalline]]		*[[Crystalline\|Crystalline]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009369472<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]

OCA at 06:59, 20 October 2012

OCA — Sat, 20 Oct 2012 06:59:03 GMT

←Older revision		Revision as of 06:59, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1auw]] is a 4 chain structure ~~of [[Crystalline]]~~ with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA].	+	[[1auw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUW OCA].

	==See Also==		==See Also==

OCA: Protected "1auw" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 14:55:47 GMT

Protected "1auw" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:55, 25 July 2012