1ax7 - Revision history

OCA at 11:34, 22 November 2023

OCA — Wed, 22 Nov 2023 11:34:07 GMT

←Older revision		Revision as of 11:34, 22 November 2023
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES==		==SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES==
-	<StructureSection load='1ax7' size='340' side='right'caption='[[1ax7~~]], [[NMR_Ensembles_of_Models \| 6 NMR models~~]]' scene=''>	+	<StructureSection load='1ax7' size='340' side='right'caption='[[1ax7]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [https://pdbe.org/1ax7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ax7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ax7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [https://pdbe.org/1ax7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ax7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ax7 ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Broyde, S]]	+	[[Category: Broyde S]]
-	[[Category: Gorin~~, A A~~]]	+	[[Category: Gorin AA]]
-	[[Category: Gu, Z]]	+	[[Category: Gu Z]]
-	[[Category: Hingerty~~, B E~~]]	+	[[Category: Hingerty BE]]
-	[[Category: Mao, B]]	+	[[Category: Mao B]]
-	[[Category: Patel~~, D J]]~~	+	[[Category: Patel DJ]]
-	~~[[Category: Aminofluorene adduct]]~~	+
-	~~[[Category: Carcinogen adduct]]~~	+
-	~~[[Category: Dna]]~~	+
-	~~[[Category: Dna duplex]]~~	+
-	~~[[Category: Template-primer junction~~]]	+

OCA at 06:55, 24 February 2021

OCA — Wed, 24 Feb 2021 06:55:13 GMT

←Older revision		Revision as of 06:55, 24 February 2021
Line 3:		Line 3:
	<StructureSection load='1ax7' size='340' side='right'caption='[[1ax7]], [[NMR_Ensembles_of_Models \| 6 NMR models]]' scene=''>		<StructureSection load='1ax7' size='340' side='right'caption='[[1ax7]], [[NMR_Ensembles_of_Models \| 6 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [~~http~~://pdbe.org/1ax7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ax7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ax7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [https://pdbe.org/1ax7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ax7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ax7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:37, 21 May 2019

OCA — Tue, 21 May 2019 08:37:21 GMT

←Older revision		Revision as of 08:37, 21 May 2019
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES==		==SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES==
-	<StructureSection load='1ax7' size='340' side='right' caption='[[1ax7]], [[NMR_Ensembles_of_Models \| 6 NMR models]]' scene=''>	+	<StructureSection load='1ax7' size='340' side='right'caption='[[1ax7]], [[NMR_Ensembles_of_Models \| 6 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Broyde, S]]		[[Category: Broyde, S]]
	[[Category: Gorin, A A]]		[[Category: Gorin, A A]]

OCA at 06:04, 17 August 2017

OCA — Thu, 17 Aug 2017 06:04:08 GMT

←Older revision		Revision as of 06:04, 17 August 2017
Line 1:		Line 1:
		+
	==SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES==		==SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES==
	<StructureSection load='1ax7' size='340' side='right' caption='[[1ax7]], [[NMR_Ensembles_of_Models \| 6 NMR models]]' scene=''>		<StructureSection load='1ax7' size='340' side='right' caption='[[1ax7]], [[NMR_Ensembles_of_Models \| 6 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [http://pdbe.org/1ax7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ax7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [http://pdbe.org/1ax7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ax7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ax7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:30, 10 September 2015

OCA — Thu, 10 Sep 2015 21:30:00 GMT

←Older revision		Revision as of 21:30, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ax7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [http://pdbe.org/1ax7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ax7 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ax7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:27, 22 December 2014

OCA — Mon, 22 Dec 2014 11:27:04 GMT

←Older revision		Revision as of 11:27, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ax7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ax7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Broyde, S.]]	+	[[Category: Broyde, S]]
-	[[Category: Gorin, A A.]]	+	[[Category: Gorin, A A]]
-	[[Category: Gu, Z.]]	+	[[Category: Gu, Z]]
-	[[Category: Hingerty, B E.]]	+	[[Category: Hingerty, B E]]
-	[[Category: Mao, B.]]	+	[[Category: Mao, B]]
-	[[Category: Patel, D J.]]	+	[[Category: Patel, D J]]
	[[Category: Aminofluorene adduct]]		[[Category: Aminofluorene adduct]]
	[[Category: Carcinogen adduct]]		[[Category: Carcinogen adduct]]

OCA at 03:10, 7 August 2014

OCA — Thu, 07 Aug 2014 03:10:00 GMT

←Older revision		Revision as of 03:10, 7 August 2014
Line 1:		Line 1:
-	[[~~Image:~~1ax7.~~png~~\|~~left\|200px~~]]	+	==SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES==
		+	<StructureSection load='1ax7' size='340' side='right' caption='[[1ax7]], [[NMR_Ensembles_of_Models \| 6 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AX7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ax7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ax7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ax7 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A solution structural study has been undertaken on the aminofluorene-C8-dG ([AF]dG) adduct located at a single strand-double strand d(A1-A2-C3-[AF]G4-C5-T6-A7-C8-C9-A10-T11-C12-C13).d (G14-G15-A16-T17-G18-G19-T20-A 21-G22) 13/9-mer junction (designated [AF]dG 13/9-mer) using proton-proton distance and intensity restraints derived from NMR data in combination with a computational protocol, which includes intensity refinement. This single strand-double strand junction models one arm of a replication fork composed of a 13-mer template strand, which contains the [AF]dG modification site, and a 9-mer primer strand, which has been elongated up to, but not including, the modified guanine. The NMR data establish that the duplex segment retains a minimally perturbed B-DNA conformation including Watson-Crick hydrogen-bonding at the junctional dC5.dG22 base pair. The NMR spectra are consistent with the guanine ring of the [AF]dG4 adduct adopting a syn glycosidic torsion angle and being displaced into the major groove with the adjacent dC3 residue displaced into the minor groove. Such a base displacement of the modified guanine is accompanied by stacking of one face of the fluorene ring of [AF]dG4 with the dC5.dG22 base pair, while the other face of the flourene ring is stacked with the purine ring of the nonadjacent dA2 residue in the intensity-refined solution structures of the [AF]dG 13/9-mer. A comparison of structural features of the C8-[AF]dG adduct (this study) with those of the (+)-trans-anti-N2-[BP]dG adduct [Cosman et al. (1995) Biochemistry 34, 15334-15350] in the same 13/9-mer junctional sequence context has identified common features associated with the alignment of the modified guanine adducts at the template-primer junction. Thus, despite differences in the covalent linkage site for the C8-[AF]dG and (+)-trans-anti-N2-[BP]dG adducts, one face of the aromatic ring of the carcinogen stacks over the junctional base pair and in so doing displaces the modified guanine in a syn alignment into the major groove. These results lend credence to earlier proposals that such an adduct alignment may represent a common mutagenic conformer at a template-primer junction associated with a replication fork.

-	~~{{STRUCTURE_1ax7\| PDB=1ax7 \| SCENE= }}~~	+	Solution structure of the aminofluorene-stacked conformer of the syn [AF]-C8-dG adduct positioned at a template-primer junction.,Mao B, Gu Z, Gorin A, Hingerty BE, Broyde S, Patel DJ Biochemistry. 1997 Nov 25;36(47):14491-501. PMID:9398168<ref>PMID:9398168</ref>

-	~~===SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION~~, ~~NMR, 6 STRUCTURES===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9398168}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1ax7]] is a 2 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA].~~	+	</StructureSection>
	[[Category: Broyde, S.]]		[[Category: Broyde, S.]]
	[[Category: Gorin, A A.]]		[[Category: Gorin, A A.]]

OCA: Protected "1ax7" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 11:44:02 GMT

Protected "1ax7" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:44, 21 October 2012

OCA at 11:44, 21 October 2012

OCA — Sun, 21 Oct 2012 11:44:00 GMT

←Older revision		Revision as of 11:44, 21 October 2012
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-	{{Seed}}
	[[Image:1ax7.png\|left\|200px]]		[[Image:1ax7.png\|left\|200px]]

-	<!--
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-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_1ax7\| PDB=1ax7 \| SCENE= }}		{{STRUCTURE_1ax7\| PDB=1ax7 \| SCENE= }}

	===SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES===		===SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9398168}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9398168 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_9398168}}		{{ABSTRACT_PUBMED_9398168}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA].	+	[[1ax7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA].
-		+
-	~~==Reference==~~	+
-	Solution structure of the aminofluorene-stacked conformer of the syn [AF]-C8-dG adduct positioned at a template-primer junction., Mao B, Gu Z, Gorin A, Hingerty BE, Broyde S, Patel DJ, Biochemistry. 1997 Nov 25;36(47):14491-501. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9398168 9398168]	+
	[[Category: Broyde, S.]]		[[Category: Broyde, S.]]
	[[Category: Gorin, A A.]]		[[Category: Gorin, A A.]]
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	[[Category: Aminofluorene adduct]]		[[Category: Aminofluorene adduct]]
	[[Category: Carcinogen adduct]]		[[Category: Carcinogen adduct]]
		+	[[Category: Dna]]
	[[Category: Dna duplex]]		[[Category: Dna duplex]]
	[[Category: Template-primer junction]]		[[Category: Template-primer junction]]
-
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OCA — Mon, 30 Jun 2008 14:46:56 GMT

←Older revision		Revision as of 14:46, 30 June 2008
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	{{STRUCTURE_1ax7\| PDB=1ax7 \| SCENE= }}		{{STRUCTURE_1ax7\| PDB=1ax7 \| SCENE= }}

-	~~'''~~SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES~~'''~~	+	===SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED AT A TEMPLATE-PRIMER JUNCTION, NMR, 6 STRUCTURES===


-	~~==Overview==~~	+	<!--
-	~~A solution structural study has been undertaken on the aminofluorene~~-C8-dG ([AF]dG) adduct located at a single strand-double strand d(A1-A2-C3-[AF]G4-C5-T6-A7-C8-C9-A10-T11-C12-C13).d (G14-G15-A16-T17-G18-G19-T20-A 21-G22) 13/9-mer junction (designated [AF]dG 13/9-mer) using proton-proton distance and intensity restraints derived from NMR data in combination with a computational protocol, ~~which includes intensity refinement. This single strand-double strand junction models one arm of a replication fork composed of a 13-mer template strand~~, ~~which contains~~ the ~~[AF]dG modification site, and a 9-mer primer strand, which has been elongated up~~ to~~, but not including,~~ the ~~modified guanine. The NMR data establish that the duplex segment retains a minimally perturbed B-DNA conformation including Watson-Crick hydrogen-bonding~~ at the junctional dC5.dG22 base pair. The NMR spectra are consistent with the guanine ring of the [AF]dG4 adduct adopting a syn glycosidic torsion angle and being displaced into the major groove with the adjacent dC3 residue displaced into the minor groove. Such a base displacement of the modified guanine is accompanied by stacking of one face of the fluorene ring of [AF]dG4 with the dC5.dG22 base pair, while the other face of the flourene ring is stacked with the purine ring of the nonadjacent dA2 residue in the intensity-refined solution structures of the [AF]dG 13/~~9-mer~~. ~~A comparison of structural features of the C8-[AF]dG adduct (this study) with those of the (+)-trans-anti-N2-[BP]dG adduct [Cosman et al~~. ~~(1995~~) ~~Biochemistry 34~~, ~~15334-15350] in~~ the ~~same 13/9-mer junctional sequence context has identified common features associated with the alignment of the modified guanine adducts at the template-primer junction~~. ~~Thus, despite differences in the covalent linkage site for the C8~~-~~[AF]dG and (+)~~-trans-anti-N2-[BP]dG adducts, one face of the aromatic ring of the carcinogen stacks over the junctional base pair and in so doing displaces the modified guanine in a syn alignment into the major groove. These results lend credence to earlier proposals that such an adduct alignment may represent a common mutagenic conformer at a template-primer junction associated with a replication fork.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9398168}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9398168 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9398168}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AX7 OCA].

	==Reference==		==Reference==
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	[[Category: Dna duplex]]		[[Category: Dna duplex]]
	[[Category: Template-primer junction]]		[[Category: Template-primer junction]]
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