1ayi - Revision history

OCA at 06:34, 7 February 2024

OCA — Wed, 07 Feb 2024 06:34:04 GMT

←Older revision		Revision as of 06:34, 7 February 2024
Line 3:		Line 3:
	<StructureSection load='1ayi' size='340' side='right'caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ayi' size='340' side='right'caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AYI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [https://pdbe.org/1ayi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [https://www.ebi.ac.uk/pdbsum/1ayi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ayi ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [https://pdbe.org/1ayi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [https://www.ebi.ac.uk/pdbsum/1ayi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ayi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.	+	[https://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ayi ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ayi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We report the first detailed comparison of two immunity proteins which, in conjunction with recent protein engineering data, begins to explain how these structurally similar proteins are able to bind and inhibit the endonuclease domain of colicin E9 (E9 DNase) with affinities that differ by 12 orders of magnitude. In the present work, we have determined the X-ray structure of the Escherichia coli colicin E7 immunity protein Im7 to 2.0 A resolution by molecular replacement, using as a trial model the recently determined NMR solution structure of Im9. Whereas the two proteins adopt similar four-helix structures, subtle structural differences, in particular involving a conserved tyrosine residue critical for E9 DNase binding, and the identity of key residues in the specificity helix, lie at the heart of their markedly different ability to bind the E9 DNase. Two other crystal structures were reported recently for Im7; in one, Im7 was a monomer and was very similar to the structure reported here, whereas in the other it was a dimer to which functional significance was assigned. Since this previous work suggested that Im7 could exist either as a monomer or a dimer, we used analytical ultracentrifugation to investigate this question further. Under a variety of solution conditions, we found that Im7 only ever exists in solution as a monomer, even up to protein concentrations of 15 mg/ml, casting doubt on the functional significance of the crystallographically observed dimer. This work provides a structural framework with which we can understand immunity-protein specificity, and in addition we believe it to be the first successfully refined crystal structure solved by molecular replacement using an NMR trial model with less than 100% sequence identity.
-
-	A structural comparison of the colicin immunity proteins Im7 and Im9 gives new insights into the molecular determinants of immunity-protein specificity.,Dennis CA, Videler H, Pauptit RA, Wallis R, James R, Moore GR, Kleanthous C Biochem J. 1998 Jul 1;333 ( Pt 1):183-91. PMID:9639578<ref>PMID:9639578</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ayi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Colicin immunity protein 3D structures\|Colicin immunity protein 3D structures]]		*[[Colicin immunity protein 3D structures\|Colicin immunity protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dennis~~, C A~~]]	+	[[Category: Dennis CA]]
-	[[Category: James, R]]	+	[[Category: James R]]
-	[[Category: Kleanthous, C]]	+	[[Category: Kleanthous C]]
-	[[Category: Moore~~, G R~~]]	+	[[Category: Moore GR]]
-	[[Category: Pauptit~~, R A~~]]	+	[[Category: Pauptit RA]]
-	[[Category: Wallis, R]]	+	[[Category: Wallis R]]
-	~~[[Category: Bacteriocin]]~~	+
-	~~[[Category: Dnase inhibitor]]~~	+
-	~~[[Category: E colicin]]~~	+
-	~~[[Category: Protein-protein interaction~~]]	+

OCA at 15:02, 2 June 2021

OCA — Wed, 02 Jun 2021 15:02:39 GMT

←Older revision		Revision as of 15:02, 2 June 2021
Line 3:		Line 3:
	<StructureSection load='1ayi' size='340' side='right'caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ayi' size='340' side='right'caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AYI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [~~http~~://pdbe.org/1ayi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ayi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ayi ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [https://pdbe.org/1ayi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [https://www.ebi.ac.uk/pdbsum/1ayi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ayi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.	+	[[https://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:23, 28 August 2019

OCA — Wed, 28 Aug 2019 16:23:27 GMT

←Older revision		Revision as of 16:23, 28 August 2019
Line 1:		Line 1:

	==COLICIN E7 IMMUNITY PROTEIN IM7==		==COLICIN E7 IMMUNITY PROTEIN IM7==
-	<StructureSection load='1ayi' size='340' side='right' caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1ayi' size='340' side='right'caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AYI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AYI FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ay/1ayi_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ay/1ayi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1ayi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ayi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Colicin immunity protein 3D structures\|Colicin immunity protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Dennis, C A]]		[[Category: Dennis, C A]]
	[[Category: James, R]]		[[Category: James, R]]

OCA at 11:11, 22 November 2017

OCA — Wed, 22 Nov 2017 11:11:32 GMT

←Older revision		Revision as of 11:11, 22 November 2017
Line 1:		Line 1:
		+
	==COLICIN E7 IMMUNITY PROTEIN IM7==		==COLICIN E7 IMMUNITY PROTEIN IM7==
	<StructureSection load='1ayi' size='340' side='right' caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ayi' size='340' side='right' caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AYI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AYI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [http://pdbe.org/1ayi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [http://www.ebi.ac.uk/pdbsum/1ayi PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [http://pdbe.org/1ayi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [http://www.ebi.ac.uk/pdbsum/1ayi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ayi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1ayi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ayi" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Colicin Immunity Protein\|Colicin Immunity Protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:46, 9 February 2016

OCA — Tue, 09 Feb 2016 05:46:14 GMT

←Older revision		Revision as of 05:46, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ayi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:12, 10 September 2015

OCA — Thu, 10 Sep 2015 19:12:47 GMT

←Older revision		Revision as of 19:12, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1ayi' size='340' side='right' caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ayi' size='340' side='right' caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AYI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [http://www.ebi.ac.uk/pdbsum/1ayi PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [http://pdbe.org/1ayi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [http://www.ebi.ac.uk/pdbsum/1ayi PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ayi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Dennis, C A]]		[[Category: Dennis, C A]]
	[[Category: James, R]]		[[Category: James, R]]

OCA at 04:49, 25 December 2014

OCA — Thu, 25 Dec 2014 04:49:36 GMT

←Older revision		Revision as of 04:49, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [http://www.ebi.ac.uk/pdbsum/1ayi PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [http://www.ebi.ac.uk/pdbsum/1ayi PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:37, 22 December 2014

OCA — Mon, 22 Dec 2014 08:37:01 GMT

←Older revision		Revision as of 08:37, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AYI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AYI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [http://www.ebi.ac.uk/pdbsum/1ayi PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [http://www.ebi.ac.uk/pdbsum/1ayi PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Dennis, C A.]]	+	[[Category: Dennis, C A]]
-	[[Category: James, R.]]	+	[[Category: James, R]]
-	[[Category: Kleanthous, C.]]	+	[[Category: Kleanthous, C]]
-	[[Category: Moore, G R.]]	+	[[Category: Moore, G R]]
-	[[Category: Pauptit, R A.]]	+	[[Category: Pauptit, R A]]
-	[[Category: Wallis, R.]]	+	[[Category: Wallis, R]]
	[[Category: Bacteriocin]]		[[Category: Bacteriocin]]
	[[Category: Dnase inhibitor]]		[[Category: Dnase inhibitor]]
	[[Category: E colicin]]		[[Category: E colicin]]
	[[Category: Protein-protein interaction]]		[[Category: Protein-protein interaction]]

OCA at 03:14, 7 August 2014

OCA — Thu, 07 Aug 2014 03:14:40 GMT

←Older revision		Revision as of 03:14, 7 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1ayi.~~png\|left~~\|200px]]	+	==COLICIN E7 IMMUNITY PROTEIN IM7==
		+	<StructureSection load='1ayi' size='340' side='right' caption='[[1ayi]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AYI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [http://www.ebi.ac.uk/pdbsum/1ayi PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ay/1ayi_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We report the first detailed comparison of two immunity proteins which, in conjunction with recent protein engineering data, begins to explain how these structurally similar proteins are able to bind and inhibit the endonuclease domain of colicin E9 (E9 DNase) with affinities that differ by 12 orders of magnitude. In the present work, we have determined the X-ray structure of the Escherichia coli colicin E7 immunity protein Im7 to 2.0 A resolution by molecular replacement, using as a trial model the recently determined NMR solution structure of Im9. Whereas the two proteins adopt similar four-helix structures, subtle structural differences, in particular involving a conserved tyrosine residue critical for E9 DNase binding, and the identity of key residues in the specificity helix, lie at the heart of their markedly different ability to bind the E9 DNase. Two other crystal structures were reported recently for Im7; in one, Im7 was a monomer and was very similar to the structure reported here, whereas in the other it was a dimer to which functional significance was assigned. Since this previous work suggested that Im7 could exist either as a monomer or a dimer, we used analytical ultracentrifugation to investigate this question further. Under a variety of solution conditions, we found that Im7 only ever exists in solution as a monomer, even up to protein concentrations of 15 mg/ml, casting doubt on the functional significance of the crystallographically observed dimer. This work provides a structural framework with which we can understand immunity-protein specificity, and in addition we believe it to be the first successfully refined crystal structure solved by molecular replacement using an NMR trial model with less than 100% sequence identity.

-	~~{{STRUCTURE_1ayi\| PDB=1ayi \| SCENE= }}~~	+	A structural comparison of the colicin immunity proteins Im7 and Im9 gives new insights into the molecular determinants of immunity-protein specificity.,Dennis CA, Videler H, Pauptit RA, Wallis R, James R, Moore GR, Kleanthous C Biochem J. 1998 Jul 1;333 ( Pt 1):183-91. PMID:9639578<ref>PMID:9639578</ref>

-	~~===COLICIN E7 IMMUNITY PROTEIN IM7===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9639578}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1ayi]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA].~~	+

	==See Also==		==See Also==
	*[[Colicin Immunity Protein\|Colicin Immunity Protein]]		*[[Colicin Immunity Protein\|Colicin Immunity Protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009639578<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Dennis, C A.]]		[[Category: Dennis, C A.]]

OCA at 12:50, 20 October 2012

OCA — Sat, 20 Oct 2012 12:50:49 GMT

←Older revision		Revision as of 12:50, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1ayi]] is a 1 chain structure ~~of [[Colicin Immunity Protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA].	+	[[1ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA].

	==See Also==		==See Also==