1b2s - Revision history

OCA at 05:35, 9 August 2023

OCA — Wed, 09 Aug 2023 05:35:18 GMT

←Older revision		Revision as of 05:35, 9 August 2023
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	<StructureSection load='1b2s' size='340' side='right'caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='1b2s' size='340' side='right'caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B2S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B2S FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.82Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [https://pdbe.org/1b2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [https://www.ebi.ac.uk/pdbsum/1b2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [https://pdbe.org/1b2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [https://www.ebi.ac.uk/pdbsum/1b2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates~~. [[https://www.uniprot.org/uniprot/BARS_BACAM BARS_BACAM]] Inhibitor of the ribonuclease barnase. Forms a one-to-one non-covalent complex~~.	+	[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Buckle~~, A M~~]]	+	[[Category: Buckle AM]]
-	[[Category: Fersht~~, A R~~]]	+	[[Category: Fersht AR]]
-	[[Category: Vaughan~~, C K]]~~	+	[[Category: Vaughan CK]]
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Interfacial double mutant]]~~	+
-	~~[[Category: Rnase-inhibitor complex~~]]	+

OCA at 10:39, 19 May 2021

OCA — Wed, 19 May 2021 10:39:10 GMT

←Older revision		Revision as of 10:39, 19 May 2021
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	<StructureSection load='1b2s' size='340' side='right'caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='1b2s' size='340' side='right'caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1B2S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B2S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [~~http~~://pdbe.org/1b2s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1b2s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2s ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [https://pdbe.org/1b2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [https://www.ebi.ac.uk/pdbsum/1b2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates. [[~~http~~://www.uniprot.org/uniprot/BARS_BACAM BARS_BACAM]] Inhibitor of the ribonuclease barnase. Forms a one-to-one non-covalent complex.	+	[[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates. [[https://www.uniprot.org/uniprot/BARS_BACAM BARS_BACAM]] Inhibitor of the ribonuclease barnase. Forms a one-to-one non-covalent complex.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	*[[Barnase 3D structures\|Barnase 3D structures]]		*[[Barnase 3D structures\|Barnase 3D structures]]
	*[[Barstar 3D structures\|Barstar 3D structures]]		*[[Barstar 3D structures\|Barstar 3D structures]]
-	*[[Ribonuclease\|Ribonuclease]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Temp\|Temp]]	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:45, 14 August 2019

OCA — Wed, 14 Aug 2019 17:45:19 GMT

←Older revision		Revision as of 17:45, 14 August 2019
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	==STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE==		==STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE==
-	<StructureSection load='1b2s' size='340' side='right' caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>	+	<StructureSection load='1b2s' size='340' side='right'caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2S FirstGlance]. <br>		<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2S FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/1b2s_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/1b2s_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:

	==See Also==		==See Also==
		+	*[[Barnase 3D structures\|Barnase 3D structures]]
		+	*[[Barstar 3D structures\|Barstar 3D structures]]
		+	*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==
Line 36:		Line 39:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Buckle, A M]]		[[Category: Buckle, A M]]
	[[Category: Fersht, A R]]		[[Category: Fersht, A R]]

OCA at 10:45, 8 November 2017

OCA — Wed, 08 Nov 2017 10:45:41 GMT

←Older revision		Revision as of 10:45, 8 November 2017
Line 1:		Line 1:
		+
	==STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE==		==STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE==
	<StructureSection load='1b2s' size='340' side='right' caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='1b2s' size='340' side='right' caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [http://pdbe.org/1b2s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [http://www.ebi.ac.uk/pdbsum/1b2s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [http://pdbe.org/1b2s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [http://www.ebi.ac.uk/pdbsum/1b2s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	==See Also==		==See Also==
-	*[[Barnase\|Barnase]]
-	*[[Barstar\|Barstar]]
-	*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==

OCA at 12:20, 7 February 2016

OCA — Sun, 07 Feb 2016 12:20:57 GMT

←Older revision		Revision as of 12:20, 7 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b2s ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:27, 10 September 2015

OCA — Thu, 10 Sep 2015 01:27:45 GMT

←Older revision		Revision as of 01:27, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1b2s' size='340' side='right' caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='1b2s' size='340' side='right' caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_amyloliquefaciens Bacillus amyloliquefaciens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [http://www.ebi.ac.uk/pdbsum/1b2s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [http://pdbe.org/1b2s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [http://www.ebi.ac.uk/pdbsum/1b2s PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1b2s" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Buckle, A M]]		[[Category: Buckle, A M]]
	[[Category: Fersht, A R]]		[[Category: Fersht, A R]]

OCA at 14:50, 15 February 2015

OCA — Sun, 15 Feb 2015 14:50:43 GMT

←Older revision		Revision as of 14:50, 15 February 2015
Line 33:		Line 33:
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
-	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:03, 24 December 2014

OCA — Wed, 24 Dec 2014 15:03:49 GMT

←Older revision		Revision as of 15:03, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [http://www.ebi.ac.uk/pdbsum/1b2s PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [http://www.ebi.ac.uk/pdbsum/1b2s PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates. [[http://www.uniprot.org/uniprot/BARS_BACAM BARS_BACAM]] Inhibitor of the ribonuclease barnase. Forms a one-to-one non-covalent complex.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:36, 22 December 2014

OCA — Mon, 22 Dec 2014 09:36:04 GMT

←Older revision		Revision as of 09:36, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2S FirstGlance]. <br>		<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2S FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [http://www.ebi.ac.uk/pdbsum/1b2s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [http://www.ebi.ac.uk/pdbsum/1b2s PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	*[[Barstar\|Barstar]]		*[[Barstar\|Barstar]]
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
		+	*[[Temp\|Temp]]
		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
-	[[Category: Buckle, A M.]]	+	[[Category: Buckle, A M]]
-	[[Category: Fersht, A R.]]	+	[[Category: Fersht, A R]]
-	[[Category: Vaughan, C K.]]	+	[[Category: Vaughan, C K]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Interfacial double mutant]]		[[Category: Interfacial double mutant]]
	[[Category: Rnase-inhibitor complex]]		[[Category: Rnase-inhibitor complex]]

OCA at 03:11, 7 August 2014

OCA — Thu, 07 Aug 2014 03:11:34 GMT

←Older revision		Revision as of 03:11, 7 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1b2s.~~png\|left~~\|200px]]	+	==STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE==
		+	<StructureSection load='1b2s' size='340' side='right' caption='[[1b2s]], [[Resolution\|resolution]] 1.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1b2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2S FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2s RCSB], [http://www.ebi.ac.uk/pdbsum/1b2s PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/1b2s_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have crystallised three mutants of the barnase-barstar complex in which interactions across the interface have been deleted by simultaneous mutation of both residues involved in the interaction. Each mutant deletes a different type of interaction at the interface: the first complex bnHis102-->Ala-bsTyr29-->Phe (bn, barnase; bs, barstar), deletes a van der Waals packing interaction; the second complex, bnLys27-->Ala-bsThr42-->Ala, deletes a hydrogen bond; the third, bnLys27-->Ala-bsAsp35-->Ala, deletes a long-range charge-charge interaction. The contribution of each of these side-chains to the stability of the complex is known; the coupling energy between the deleted side-chains is also known. Despite each of the double mutants being significantly destabilised compared with the wild-type, the effects of mutation are local. Only small movements in the main-chain surrounding the sites of mutation and some larger movements of neighbouring side-chains are observed in the mutant complexes. The exact response to mutation is context-dependent and for the same mutant can vary depending upon the environment within the crystal. In some double mutant complexes, interfacial pockets, which are accessible to bulk solvent are formed, whereas interfacial cavities which are isolated from bulk solvent, are formed in others. In all double mutants, water molecules fill the created pockets and cavities. These water molecules mimic the deleted side-chains by occupying positions close to the non-carbon atoms of truncated side-chains and re-making many hydrogen bonds made by the truncated side-chains in the wild-type. It remains extremely difficult, however, to correlate energetic and structural responses to mutation because of unknown changes in entropy and entropy-enthalpy compensation.

-	~~{{STRUCTURE_1b2s\| PDB=1b2s \| SCENE= }}~~	+	Structural response to mutation at a protein-protein interface.,Vaughan CK, Buckle AM, Fersht AR J Mol Biol. 1999 Mar 12;286(5):1487-506. PMID:10064711<ref>PMID:10064711</ref>

-	~~===STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10064711}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1b2s]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1B2S OCA].~~	+

	==See Also==		==See Also==
Line 14:		Line 30:
	*[[Barstar\|Barstar]]		*[[Barstar\|Barstar]]
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010064711<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
	[[Category: Buckle, A M.]]		[[Category: Buckle, A M.]]