1b2w - Revision history

OCA at 05:35, 9 August 2023

OCA — Wed, 09 Aug 2023 05:35:24 GMT

←Older revision		Revision as of 05:35, 9 August 2023
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	<StructureSection load='1b2w' size='340' side='right'caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1b2w' size='340' side='right'caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B2W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B2W FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [https://pdbe.org/1b2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [https://www.ebi.ac.uk/pdbsum/1b2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [https://pdbe.org/1b2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [https://www.ebi.ac.uk/pdbsum/1b2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2w ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Co~~, M S~~]]	+	[[Category: Co MS]]
-	[[Category: Edmundson~~, A B~~]]	+	[[Category: Edmundson AB]]
-	[[Category: Fan, Z]]	+	[[Category: Fan Z]]
-	[[Category: Goldsteen~~, B Z~~]]	+	[[Category: Goldsteen BZ]]
-	[[Category: Guddat~~, L W~~]]	+	[[Category: Guddat LW]]
-	[[Category: Landolfi~~, N F~~]]	+	[[Category: Landolfi NF]]
-	[[Category: Queen, C]]	+	[[Category: Queen C]]
-	[[Category: Ramsland~~, P A~~]]	+	[[Category: Ramsland PA]]
-	[[Category: Shan, L]]	+	[[Category: Shan L]]
-	[[Category: Thakur, A]]	+	[[Category: Thakur A]]
-	[[Category: Vasquez, M]]	+	[[Category: Vasquez M]]
-	~~[[Category: Antibody engineering]]~~	+
-	~~[[Category: Fab]]~~	+
-	~~[[Category: Gamma-interferon]]~~	+
-	~~[[Category: Humanized and chimeric antibody]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Three-dimensional strycture~~]]	+

OCA at 09:16, 29 September 2021

OCA — Wed, 29 Sep 2021 09:16:38 GMT

←Older revision		Revision as of 09:16, 29 September 2021
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	<StructureSection load='1b2w' size='340' side='right'caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1b2w' size='340' side='right'caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1B2W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B2W FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [~~http~~://pdbe.org/1b2w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1b2w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [https://pdbe.org/1b2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [https://www.ebi.ac.uk/pdbsum/1b2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2w ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:
	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Sandbox 20009\|Sandbox 20009]]
		+	*[[3D structures of human antibody\|3D structures of human antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:45, 11 December 2019

OCA — Wed, 11 Dec 2019 19:45:40 GMT

←Older revision		Revision as of 19:45, 11 December 2019
Line 1:		Line 1:

	==COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY==		==COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY==
-	<StructureSection load='1b2w' size='340' side='right' caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='1b2w' size='340' side='right'caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>		<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Co, M S]]		[[Category: Co, M S]]

OCA at 05:23, 8 March 2018

OCA — Thu, 08 Mar 2018 05:23:47 GMT

←Older revision		Revision as of 05:23, 8 March 2018
Line 3:		Line 3:
	<StructureSection load='1b2w' size='340' side='right' caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1b2w' size='340' side='right' caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [http://pdbe.org/1b2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [http://www.ebi.ac.uk/pdbsum/1b2w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [http://pdbe.org/1b2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [http://www.ebi.ac.uk/pdbsum/1b2w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2w ProSAT]</span></td></tr>
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/1b2w_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/1b2w_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:

	==See Also==		==See Also==
-	*[[3D structures ~~of antibody~~\|3D structures ~~of antibody~~]]	+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Co, M S]]		[[Category: Co, M S]]
	[[Category: Edmundson, A B]]		[[Category: Edmundson, A B]]

OCA at 06:16, 17 August 2017

OCA — Thu, 17 Aug 2017 06:16:35 GMT

←Older revision		Revision as of 06:16, 17 August 2017
Line 1:		Line 1:
		+
	==COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY==		==COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY==
	<StructureSection load='1b2w' size='340' side='right' caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1b2w' size='340' side='right' caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [http://pdbe.org/1b2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [http://www.ebi.ac.uk/pdbsum/1b2w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [http://pdbe.org/1b2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [http://www.ebi.ac.uk/pdbsum/1b2w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2w ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b2w ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:

	==See Also==		==See Also==
-	*[[~~Antibody~~\|~~Antibody~~]]	+	*[[3D structures of antibody\|3D structures of antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Lk3 transgenic mice]]
	[[Category: Co, M S]]		[[Category: Co, M S]]
	[[Category: Edmundson, A B]]		[[Category: Edmundson, A B]]

OCA at 02:40, 12 September 2015

OCA — Sat, 12 Sep 2015 02:40:31 GMT

←Older revision		Revision as of 02:40, 12 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>		<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [http://www.ebi.ac.uk/pdbsum/1b2w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [http://pdbe.org/1b2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [http://www.ebi.ac.uk/pdbsum/1b2w PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1b2w" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 12:08, 22 December 2014

OCA — Mon, 22 Dec 2014 12:08:45 GMT

←Older revision		Revision as of 12:08, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>		<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [http://www.ebi.ac.uk/pdbsum/1b2w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [http://www.ebi.ac.uk/pdbsum/1b2w PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
-	[[Category: Co, M S.]]	+	[[Category: Co, M S]]
-	[[Category: Edmundson, A B.]]	+	[[Category: Edmundson, A B]]
-	[[Category: Fan, Z.]]	+	[[Category: Fan, Z]]
-	[[Category: Goldsteen, B Z.]]	+	[[Category: Goldsteen, B Z]]
-	[[Category: Guddat, L W.]]	+	[[Category: Guddat, L W]]
-	[[Category: Landolfi, N F.]]	+	[[Category: Landolfi, N F]]
-	[[Category: Queen, C.]]	+	[[Category: Queen, C]]
-	[[Category: Ramsland, P A.]]	+	[[Category: Ramsland, P A]]
-	[[Category: Shan, L.]]	+	[[Category: Shan, L]]
-	[[Category: Thakur, A.]]	+	[[Category: Thakur, A]]
-	[[Category: Vasquez, M.]]	+	[[Category: Vasquez, M]]
	[[Category: Antibody engineering]]		[[Category: Antibody engineering]]
	[[Category: Fab]]		[[Category: Fab]]

OCA at 05:38, 3 October 2014

OCA — Fri, 03 Oct 2014 05:38:45 GMT

←Older revision		Revision as of 05:38, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1b2w\| PDB=1b2w \| SCENE= }}~~	+	==COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY==
-	===COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY===	+	<StructureSection load='1b2w' size='340' side='right' caption='[[1b2w]], [[Resolution\|resolution]] 2.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_10398393}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1b2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B2W FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b2w RCSB], [http://www.ebi.ac.uk/pdbsum/1b2w PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/1b2w_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The objective of this work is to compare the three-dimensional structures of "humanized" and mouse-human chimeric forms of a murine monoclonal antibody elicited against human gamma-interferon. It is also to provide structural explanations for the small differences in the affinities and biological interactions of the two molecules for this antigen. Antigen-binding fragments (Fabs) were produced by papain hydrolysis of the antibodies and crystallized with polyethylene glycol (PEG) 8,000 by nearly identical microseeding procedures. Their structures were solved by X-ray analyses at 2.9 A resolution, using molecular replacement methods and crystallographic refinement. Comparison of these structures revealed marked similarities in the light (L) chains and near identities of the constant (C) domains of the heavy (H) chains. However, the variable (V) domains of the heavy chains exhibited substantial differences in the conformations of all three complementarity-determining regions (CDRs), and in their first framework segments (FR1). In FR1 of the humanized VH, the substitution of serine for proline in position 7 allowed the N-terminal segment (designated strand 4-1) to be closely juxtaposed to an adjacent strand (4-2) and form hydrogen bonds typical of an antiparallel beta-pleated sheet. The tightening of the humanized structure was relayed in such a way as to decrease the space available for the last portion of HFR1 and the first part of HCDR1. This compression led to the formation of an alpha-helix involving residues 25-32. With fewer steric constraints, the corresponding segment in the chimeric Fab lengthened by at least 1 A to a random coil which terminated in a single turn of 310 helix. In the humanized Fab, HCDR1, which is sandwiched between HCDR2 and HCDR3, significantly influenced the structures of both regions. HCDR2 was forced into a bent and twisted orientation different from that in the chimeric Fab, both at the crown of the loop (around proline H52a) and at its base. As in HCDR1, the last few residues of HCDR2 in the humanized Fab were compressed into a space-saving alpha-helix, contrasting with a more extended 310 helix in the chimeric form. HCDR3 in the humanized Fab was also adjusted in shape and topography. The observed similarities in the functional binding activities of the two molecules can be rationalized by limited induced fit adjustments in their structures on antigen binding. While not perfect replicas, the two structures are testimonials to the progress in making high affinity monoclonal antibodies safe for human use.

-	~~==About this Structure==~~	+	Comparison of the three-dimensional structures of a humanized and a chimeric Fab of an anti-gamma-interferon antibody.,Fan ZC, Shan L, Goldsteen BZ, Guddat LW, Thakur A, Landolfi NF, Co MS, Vasquez M, Queen C, Ramsland PA, Edmundson AB J Mol Recognit. 1999 Jan-Feb;12(1):19-32. PMID:10398393<ref>PMID:10398393</ref>
-	~~[[1b2w]] is~~ a ~~2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann.ac.il/oca~~-~~bin/ocashort?id=1B2W OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:010398393</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[Antibody\|Antibody]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Co, M S.]]		[[Category: Co, M S.]]
	[[Category: Edmundson, A B.]]		[[Category: Edmundson, A B.]]

OCA at 09:20, 5 February 2014

OCA — Wed, 05 Feb 2014 09:20:58 GMT

←Older revision		Revision as of 09:20, 5 February 2014
Line 1:		Line 1:
-	[[Image:1b2w.png\|left\|200px]]
-
	{{STRUCTURE_1b2w\| PDB=1b2w \| SCENE= }}		{{STRUCTURE_1b2w\| PDB=1b2w \| SCENE= }}
-
	===COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY===		===COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY===
-
	{{ABSTRACT_PUBMED_10398393}}		{{ABSTRACT_PUBMED_10398393}}

Line 11:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:010398393</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010398393</ref><references group="xtra"/><references/>
	[[Category: Co, M S.]]		[[Category: Co, M S.]]
	[[Category: Edmundson, A B.]]		[[Category: Edmundson, A B.]]

OCA: Protected "1b2w" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 12:21:37 GMT

Protected "1b2w" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:21, 21 October 2012