1b3k - Revision history

OCA at 05:55, 3 April 2024

OCA — Wed, 03 Apr 2024 05:55:23 GMT

←Older revision		Revision as of 05:55, 3 April 2024
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b3k ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b3k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Plasminogen activator inhibitor 1 (PAI-1) is a serpin that has a key role in the control of fibrinolysis through proteinase inhibition. PAI-1 also has a role in regulating cell adhesion processes relevant to tissue remodeling and metastasis; this role is mediated by its binding to the adhesive glycoprotein vitronectin rather than by proteinase inhibition. Active PAI-1 is metastable and spontaneously transforms to an inactive latent conformation. Previous attempts to crystallize the active conformation of PAI-1 have failed. RESULTS: The crystal structure of a stable quadruple mutant of PAI-1(Asn150-->His, Lys154-->Thr, Gln319-->Leu, Met354-->Ile) in its active conformation has been solved at a nominal 3 A resolution. In two of four independent molecules within the crystal, the flexible reactive center loop is unconstrained by crystal-packing contacts and is disordered. In the other two molecules, the reactive center loop forms intimate loop-sheet interactions with neighboring molecules, generating an infinite chain within the crystal. The overall conformation resembles that seen for other active inhibitory serpins. CONCLUSIONS: The structure clarifies the molecular basis of the stabilizing mutations and the reduced affinity of PAI-1, on cleavage or in the latent form, for vitronectin. The infinite chain of linked molecules also suggests a new mechanism for the serpin polymerization associated with certain diseases. The results support the concept that the reactive center loop of an active serpin is flexible and has no defined conformation in the absence of intermolecular contacts. The determination of the structure of the active form constitutes an essential step for the rational design of PAI-1 inhibitors.
-
-	The active conformation of plasminogen activator inhibitor 1, a target for drugs to control fibrinolysis and cell adhesion.,Sharp AM, Stein PE, Pannu NS, Carrell RW, Berkenpas MB, Ginsburg D, Lawrence DA, Read RJ Structure. 1999 Feb 15;7(2):111-8. PMID:10368279<ref>PMID:10368279</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1b3k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 23:19, 27 December 2023

OCA — Wed, 27 Dec 2023 23:19:25 GMT

←Older revision		Revision as of 23:19, 27 December 2023
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	<StructureSection load='1b3k' size='340' side='right'caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>		<StructureSection load='1b3k' size='340' side='right'caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B3K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B3K FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>~~[[~~1c5g~~\|~~1c5g~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.99Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [https://pdbe.org/1b3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [https://www.ebi.ac.uk/pdbsum/1b3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b3k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [https://pdbe.org/1b3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [https://www.ebi.ac.uk/pdbsum/1b3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b3k ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:[https://omim.org/entry/613329 613329]]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.<ref>PMID:9207454</ref> Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.	+	[https://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN] Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:[https://omim.org/entry/613329 613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.<ref>PMID:9207454</ref> Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.<ref>PMID:15853774</ref>	+	[https://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN] Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.<ref>PMID:15853774</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pannu~~, N S~~]]	+	[[Category: Pannu NS]]
-	[[Category: Read~~, R J~~]]	+	[[Category: Read RJ]]
-	[[Category: Sharp~~, A M~~]]	+	[[Category: Sharp AM]]
-	[[Category: Stein~~, P E]]~~	+	[[Category: Stein PE]]
-	~~[[Category: Blood clotting]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Pai-1]]~~	+
-	~~[[Category: Serpin~~]]	+

OCA at 06:56, 24 February 2021

OCA — Wed, 24 Feb 2021 06:56:42 GMT

←Older revision		Revision as of 06:56, 24 February 2021
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	<StructureSection load='1b3k' size='340' side='right'caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>		<StructureSection load='1b3k' size='340' side='right'caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1B3K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B3K FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1c5g\|1c5g]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [~~http~~://pdbe.org/1b3k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1b3k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1b3k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [https://pdbe.org/1b3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [https://www.ebi.ac.uk/pdbsum/1b3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b3k ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:[~~http~~://omim.org/entry/613329 613329]]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.<ref>PMID:9207454</ref> Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.	+	[[https://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:[https://omim.org/entry/613329 613329]]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.<ref>PMID:9207454</ref> Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.<ref>PMID:15853774</ref>	+	[[https://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.<ref>PMID:15853774</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:07, 21 May 2019

OCA — Tue, 21 May 2019 09:07:34 GMT

←Older revision		Revision as of 09:07, 21 May 2019
Line 1:		Line 1:

	==Plasminogen activator inhibitor-1==		==Plasminogen activator inhibitor-1==
-	<StructureSection load='1b3k' size='340' side='right' caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>	+	<StructureSection load='1b3k' size='340' side='right'caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B3K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B3K FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [http://pdbe.org/1b3k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [http://www.ebi.ac.uk/pdbsum/1b3k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1b3k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [http://pdbe.org/1b3k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [http://www.ebi.ac.uk/pdbsum/1b3k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1b3k ProSAT]</span></td></tr>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/1b3k_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/1b3k_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 37:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Pannu, N S]]		[[Category: Pannu, N S]]
	[[Category: Read, R J]]		[[Category: Read, R J]]

OCA at 06:04, 17 August 2017

OCA — Thu, 17 Aug 2017 06:04:51 GMT

←Older revision		Revision as of 06:04, 17 August 2017
Line 1:		Line 1:
		+
	==Plasminogen activator inhibitor-1==		==Plasminogen activator inhibitor-1==
	<StructureSection load='1b3k' size='340' side='right' caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>		<StructureSection load='1b3k' size='340' side='right' caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Human Human]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B3K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B3K FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [http://pdbe.org/1b3k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [http://www.ebi.ac.uk/pdbsum/1b3k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [http://pdbe.org/1b3k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [http://www.ebi.ac.uk/pdbsum/1b3k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1b3k ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b3k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 36:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Human]]
	[[Category: Pannu, N S]]		[[Category: Pannu, N S]]
	[[Category: Read, R J]]		[[Category: Read, R J]]

OCA at 16:12, 11 September 2015

OCA — Fri, 11 Sep 2015 16:12:05 GMT

←Older revision		Revision as of 16:12, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1b3k' size='340' side='right' caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>		<StructureSection load='1b3k' size='340' side='right' caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B3K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B3K FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [http://www.ebi.ac.uk/pdbsum/1b3k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [http://pdbe.org/1b3k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [http://www.ebi.ac.uk/pdbsum/1b3k PDBsum]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1b3k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Pannu, N S]]		[[Category: Pannu, N S]]
	[[Category: Read, R J]]		[[Category: Read, R J]]

OCA at 11:53, 22 December 2014

OCA — Mon, 22 Dec 2014 11:53:17 GMT

←Older revision		Revision as of 11:53, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B3K FirstGlance]. <br>		<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B3K FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [http://www.ebi.ac.uk/pdbsum/1b3k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [http://www.ebi.ac.uk/pdbsum/1b3k PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:[http://omim.org/entry/613329 613329]]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.<ref>PMID:9207454</ref> Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.		[[http://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:[http://omim.org/entry/613329 613329]]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.<ref>PMID:9207454</ref> Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Pannu, N S.]]	+	[[Category: Pannu, N S]]
-	[[Category: Read, R J.]]	+	[[Category: Read, R J]]
-	[[Category: Sharp, A M.]]	+	[[Category: Sharp, A M]]
-	[[Category: Stein, P E.]]	+	[[Category: Stein, P E]]
	[[Category: Blood clotting]]		[[Category: Blood clotting]]
	[[Category: Inhibitor]]		[[Category: Inhibitor]]
	[[Category: Pai-1]]		[[Category: Pai-1]]
	[[Category: Serpin]]		[[Category: Serpin]]

OCA at 17:30, 29 September 2014

OCA — Mon, 29 Sep 2014 17:30:49 GMT

←Older revision		Revision as of 17:30, 29 September 2014
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-	~~{{STRUCTURE_1b3k~~\| ~~PDB~~=1b3k \| ~~SCENE~~= }}	+	==Plasminogen activator inhibitor-1==
-	===~~Plasminogen~~ activator inhibitor-1===	+	<StructureSection load='1b3k' size='340' side='right' caption='[[1b3k]], [[Resolution\|resolution]] 2.99Å' scene=''>
-	~~{{ABSTRACT_PUBMED_10368279}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1b3k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B3K FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c5g\|1c5g]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b3k RCSB], [http://www.ebi.ac.uk/pdbsum/1b3k PDBsum]</span></td></tr>
		+	<table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:[http://omim.org/entry/613329 613329]]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.<ref>PMID:9207454</ref> Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.<ref>PMID:15853774</ref>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/1b3k_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Plasminogen activator inhibitor 1 (PAI-1) is a serpin that has a key role in the control of fibrinolysis through proteinase inhibition. PAI-1 also has a role in regulating cell adhesion processes relevant to tissue remodeling and metastasis; this role is mediated by its binding to the adhesive glycoprotein vitronectin rather than by proteinase inhibition. Active PAI-1 is metastable and spontaneously transforms to an inactive latent conformation. Previous attempts to crystallize the active conformation of PAI-1 have failed. RESULTS: The crystal structure of a stable quadruple mutant of PAI-1(Asn150-->His, Lys154-->Thr, Gln319-->Leu, Met354-->Ile) in its active conformation has been solved at a nominal 3 A resolution. In two of four independent molecules within the crystal, the flexible reactive center loop is unconstrained by crystal-packing contacts and is disordered. In the other two molecules, the reactive center loop forms intimate loop-sheet interactions with neighboring molecules, generating an infinite chain within the crystal. The overall conformation resembles that seen for other active inhibitory serpins. CONCLUSIONS: The structure clarifies the molecular basis of the stabilizing mutations and the reduced affinity of PAI-1, on cleavage or in the latent form, for vitronectin. The infinite chain of linked molecules also suggests a new mechanism for the serpin polymerization associated with certain diseases. The results support the concept that the reactive center loop of an active serpin is flexible and has no defined conformation in the absence of intermolecular contacts. The determination of the structure of the active form constitutes an essential step for the rational design of PAI-1 inhibitors.

-	~~==Disease==~~	+	The active conformation of plasminogen activator inhibitor 1, a target for drugs to control fibrinolysis and cell adhesion.,Sharp AM, Stein PE, Pannu NS, Carrell RW, Berkenpas MB, Ginsburg D, Lawrence DA, Read RJ Structure. 1999 Feb 15;7(2):111-8. PMID:10368279<ref>PMID:10368279</ref>
-	~~[[http://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Defects in SERPINE1 are the cause~~ of plasminogen activator inhibitor-1 ~~deficiency (PAI-1D) [MIM:[http://omim~~.~~org/entry/613329 613329]]. It is a hematologic disorder characterized by increased bleeding after trauma~~, ~~injury~~, ~~or surgery~~. ~~Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor~~-~~1, which inhibits tissue and urinary activators of plasminogen~~.<ref>PMID:~~9207454~~</ref> ~~Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.~~	+

-	~~==Function==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[http:~~/~~/www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator~~, ~~urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as~~ a ~~major control point in~~ the ~~regulation~~ of ~~fibrinolysis~~.<~~ref~~>~~PMID:15853774~~</~~ref~~>	+	</div>
-		+
-	~~==About this Structure==~~	+
-	[[1b3k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA].	+

	==See Also==		==See Also==
	*[[Plasminogen activator inhibitor\|Plasminogen activator inhibitor]]		*[[Plasminogen activator inhibitor\|Plasminogen activator inhibitor]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:010368279</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Pannu, N S.]]		[[Category: Pannu, N S.]]

OCA at 22:13, 24 March 2013

OCA — Sun, 24 Mar 2013 22:13:52 GMT

←Older revision		Revision as of 22:13, 24 March 2013
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-	[[Image:1b3k.png\|left\|200px]]
-
	{{STRUCTURE_1b3k\| PDB=1b3k \| SCENE= }}		{{STRUCTURE_1b3k\| PDB=1b3k \| SCENE= }}
-
	===Plasminogen activator inhibitor-1===		===Plasminogen activator inhibitor-1===
		+	{{ABSTRACT_PUBMED_10368279}}

-	~~{{ABSTRACT_PUBMED_10368279}}~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:[http://omim.org/entry/613329 613329]]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.<ref>PMID:9207454</ref> Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/PAI1_HUMAN PAI1_HUMAN]] Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.<ref>PMID:15853774</ref>

	==About this Structure==		==About this Structure==
Line 14:		Line 16:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:010368279</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010368279</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Pannu, N S.]]		[[Category: Pannu, N S.]]

OCA at 20:11, 20 October 2012

OCA — Sat, 20 Oct 2012 20:11:46 GMT

←Older revision		Revision as of 20:11, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1b3k]] is a 4 chain structure ~~of [[Plasminogen activator inhibitor]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA].	+	[[1b3k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3K OCA].

	==See Also==		==See Also==