1b7j - Revision history

OCA at 05:37, 9 August 2023

OCA — Wed, 09 Aug 2023 05:37:20 GMT

←Older revision		Revision as of 05:37, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='1b7j' size='340' side='right'caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1b7j' size='340' side='right'caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Blennius_americanus Blennius~~ americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B7J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [https://pdbe.org/1b7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [https://www.ebi.ac.uk/pdbsum/1b7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b7j ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [https://pdbe.org/1b7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [https://www.ebi.ac.uk/pdbsum/1b7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b7j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ANP12_ZOAAM ANP12_ZOAAM] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Blennius americanus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baardsnes, J]]	+	[[Category: Zoarces americanus]]
-	[[Category: Davies~~, P L~~]]	+	[[Category: Baardsnes J]]
-	[[Category: Deluca~~, C I~~]]	+	[[Category: Davies PL]]
-	[[Category: Graether~~, S P~~]]	+	[[Category: Deluca CI]]
-	[[Category: Hill~~, G A~~]]	+	[[Category: Graether SP]]
-	[[Category: Jia, Z]]	+	[[Category: Hill GA]]
-	~~[[Category: Antifreeze protein]]~~	+	[[Category: Jia Z]]
-	~~[[Category: Ice binding protein]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Thermal hysteresis protein~~]]	+

OCA at 10:39, 19 May 2021

OCA — Wed, 19 May 2021 10:39:43 GMT

←Older revision		Revision as of 10:39, 19 May 2021
Line 3:		Line 3:
	<StructureSection load='1b7j' size='340' side='right'caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1b7j' size='340' side='right'caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1B7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B7J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [~~http~~://pdbe.org/1b7j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1b7j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1b7j ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [https://pdbe.org/1b7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [https://www.ebi.ac.uk/pdbsum/1b7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b7j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 17:53, 14 August 2019

OCA — Wed, 14 Aug 2019 17:53:12 GMT

←Older revision		Revision as of 17:53, 14 August 2019
Line 1:		Line 1:

	==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 V20A==		==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 V20A==
-	<StructureSection load='1b7j' size='340' side='right' caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='1b7j' size='340' side='right'caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B7J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B7J FirstGlance]. <br>
Line 10:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/1b7j_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/1b7j_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 25:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1b7j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1b7j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antifreeze protein 3D structures\|Antifreeze protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Blennius americanus]]		[[Category: Blennius americanus]]
		+	[[Category: Large Structures]]
	[[Category: Baardsnes, J]]		[[Category: Baardsnes, J]]
	[[Category: Davies, P L]]		[[Category: Davies, P L]]

OCA at 10:52, 8 November 2017

OCA — Wed, 08 Nov 2017 10:52:48 GMT

←Older revision		Revision as of 10:52, 8 November 2017
Line 1:		Line 1:
		+
	==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 V20A==		==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 V20A==
	<StructureSection load='1b7j' size='340' side='right' caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1b7j' size='340' side='right' caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B7J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B7J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [http://pdbe.org/1b7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [http://www.ebi.ac.uk/pdbsum/1b7j PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [http://pdbe.org/1b7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [http://www.ebi.ac.uk/pdbsum/1b7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1b7j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1b7j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1b7j" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Antifreeze protein\|Antifreeze protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:42, 7 February 2016

OCA — Sun, 07 Feb 2016 10:42:16 GMT

←Older revision		Revision as of 10:42, 7 February 2016
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b7j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:45, 10 September 2015

OCA — Thu, 10 Sep 2015 00:45:24 GMT

←Older revision		Revision as of 00:45, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1b7j' size='340' side='right' caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1b7j' size='340' side='right' caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Zoarces_americanus Zoarces~~ americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B7J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [http://www.ebi.ac.uk/pdbsum/1b7j PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [http://pdbe.org/1b7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [http://www.ebi.ac.uk/pdbsum/1b7j PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1b7j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Zoarces~~ americanus]]	+	[[Category: Blennius americanus]]
	[[Category: Baardsnes, J]]		[[Category: Baardsnes, J]]
	[[Category: Davies, P L]]		[[Category: Davies, P L]]

OCA at 08:46, 22 December 2014

OCA — Mon, 22 Dec 2014 08:46:07 GMT

←Older revision		Revision as of 08:46, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B7J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B7J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [http://www.ebi.ac.uk/pdbsum/1b7j PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [http://www.ebi.ac.uk/pdbsum/1b7j PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Zoarces americanus]]		[[Category: Zoarces americanus]]
-	[[Category: Baardsnes, J.]]	+	[[Category: Baardsnes, J]]
-	[[Category: Davies, P L.]]	+	[[Category: Davies, P L]]
-	[[Category: Deluca, C I.]]	+	[[Category: Deluca, C I]]
-	[[Category: Graether, S P.]]	+	[[Category: Graether, S P]]
-	[[Category: Hill, G A.]]	+	[[Category: Hill, G A]]
-	[[Category: Jia, Z.]]	+	[[Category: Jia, Z]]
	[[Category: Antifreeze protein]]		[[Category: Antifreeze protein]]
	[[Category: Ice binding protein]]		[[Category: Ice binding protein]]
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Thermal hysteresis protein]]		[[Category: Thermal hysteresis protein]]

OCA at 03:19, 7 August 2014

OCA — Thu, 07 Aug 2014 03:19:57 GMT

←Older revision		Revision as of 03:19, 7 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1b7j.~~png\|left~~\|200px]]	+	==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 V20A==
		+	<StructureSection load='1b7j' size='340' side='right' caption='[[1b7j]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B7J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1b7j RCSB], [http://www.ebi.ac.uk/pdbsum/1b7j PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/1b7j_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Some cold water marine fishes avoid cellular damage because of freezing by expressing antifreeze proteins (AFPs) that bind to ice and inhibit its growth; one such protein is the globular type III AFP from eel pout. Despite several studies, the mechanism of ice binding remains unclear because of the difficulty in modeling the AFP-ice interaction. To further explore the mechanism, we have determined the x-ray crystallographic structure of 10 type III AFP mutants and combined that information with 7 previously determined structures to mainly analyze specific AFP-ice interactions such as hydrogen bonds. Quantitative assessment of binding was performed using a neural network with properties of the structure as input and predicted antifreeze activity as output. Using the cross-validation method, a correlation coefficient of 0.60 was obtained between measured and predicted activity, indicating successful learning and good predictive power. A large loss in the predictive power of the neural network occurred after properties related to the hydrophobic surface were left out, suggesting that van der Waal's interactions make a significant contribution to ice binding. By combining the analysis of the neural network with antifreeze activity and x-ray crystallographic structures of the mutants, we extend the existing ice-binding model to a two-step process: 1) probing of the surface for the correct ice-binding plane by hydrogen-bonding side chains and 2) attractive van der Waal's interactions between the other residues of the ice-binding surface and the ice, which increases the strength of the protein-ice interaction.

-	~~{{STRUCTURE_1b7j\| PDB=1b7j \| SCENE= }}~~	+	Quantitative and qualitative analysis of type III antifreeze protein structure and function.,Graether SP, DeLuca CI, Baardsnes J, Hill GA, Davies PL, Jia Z J Biol Chem. 1999 Apr 23;274(17):11842-7. PMID:10207002<ref>PMID:10207002</ref>

-	~~===TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 V20A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10207002}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1b7j]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Zoarces_americanus Zoarces americanus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA].~~	+

	==See Also==		==See Also==
	*[[Antifreeze protein\|Antifreeze protein]]		*[[Antifreeze protein\|Antifreeze protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010207002<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Zoarces americanus]]		[[Category: Zoarces americanus]]
	[[Category: Baardsnes, J.]]		[[Category: Baardsnes, J.]]

OCA at 06:23, 20 October 2012

OCA — Sat, 20 Oct 2012 06:23:45 GMT

←Older revision		Revision as of 06:23, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1b7j]] is a 1 chain structure ~~of [[Antifreeze protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA].	+	[[1b7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7J OCA].

	==See Also==		==See Also==

OCA: Protected "1b7j" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 13:03:37 GMT

Protected "1b7j" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:03, 25 July 2012