1bne - Revision history

OCA at 15:34, 13 March 2024

OCA — Wed, 13 Mar 2024 15:34:29 GMT

←Older revision		Revision as of 15:34, 13 March 2024
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	<StructureSection load='1bne' size='340' side='right'caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bne' size='340' side='right'caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BNE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BNE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [https://pdbe.org/1bne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [https://www.ebi.ac.uk/pdbsum/1bne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bne ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [https://pdbe.org/1bne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [https://www.ebi.ac.uk/pdbsum/1bne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bne ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.	+	[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bne ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bne ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In this series of papers, we examine the effects of introducing disulfide bonds on the properties, structure and thermodynamics of a small globular protein, barnase. Three mutants have been made, in each of which a single crosslink confers different properties. Two of the disulfide bonds, between residues 43 and 80 (43-80) and between residues 85 and 102 (85-102), stabilise the protein, relative to both wild-type and the corresponding (reduced) dithiol forms: 85-102 is more stable than predicted from the entropic destabilisation of the unfolded state; 43-80 is less stable than predicted. The third disulfide bond, between residues 70 and 92 (70-92) destabilises the protein relative to both wild-type and the corresponding dithiol form, implying significant disruption of the folded protein on formation of the disulfide bond. Crystal structures of the three mutant proteins have been solved. All three proteins have essentially the same fold as wild-type, but with left-handed disulfide bonds, which have dihedral geometries that have not been observed in naturally occurring disulfides. In the very stable mutant 85-102, there is no significant difference between the mutant and wild-type structures: these data do not explain the large stability of this protein. The disulfide bond at 43-80 induces small structural rearrangements close to the site of the disulfide bond, associated with some local disorder: the crosslink appears to decrease the stability of the native form of the protein. The destabilising disulfide bond at 70-92 induces considerable structural change, with displacement of a loop and consequent disruption of a stabilising salt-bridge. Our studies do not support the view that the conformation of the disulfide bond is crucial in determining the stability of the mutant proteins.
-
-	Disulfide mutants of barnase. I: Changes in stability and structure assessed by biophysical methods and X-ray crystallography.,Clarke J, Henrick K, Fersht AR J Mol Biol. 1995 Oct 27;253(3):493-504. PMID:7473729<ref>PMID:7473729</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1bne" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
		+	*[[Barnase 3D structures\|Barnase 3D structures]]
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Clarke, J]]	+	[[Category: Clarke J]]
-	[[Category: Fersht~~, A R~~]]	+	[[Category: Fersht AR]]
-	[[Category: Henrick, K]]	+	[[Category: Henrick K]]
-	~~[[Category: Endonuclease~~]]	+

OCA at 07:01, 24 February 2021

OCA — Wed, 24 Feb 2021 07:01:05 GMT

←Older revision		Revision as of 07:01, 24 February 2021
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	<StructureSection load='1bne' size='340' side='right'caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bne' size='340' side='right'caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1BNE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BNE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [~~http~~://pdbe.org/1bne PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1bne PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1bne ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [https://pdbe.org/1bne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [https://www.ebi.ac.uk/pdbsum/1bne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bne ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.	+	[[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[~~Barnase~~ 3D structures\|~~Barnase~~ 3D structures]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Ribonuclease\|Ribonuclease]]	+
-	*[[Temp\|Temp]]	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:11, 21 May 2019

OCA — Tue, 21 May 2019 09:11:08 GMT

←Older revision		Revision as of 09:11, 21 May 2019
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	==BARNASE A43C/S80C DISULFIDE MUTANT==		==BARNASE A43C/S80C DISULFIDE MUTANT==
-	<StructureSection load='1bne' size='340' side='right' caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1bne' size='340' side='right'caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BNE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BNE FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bn/1bne_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bn/1bne_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Barnase\|Barnase]]	+	*[[Barnase 3D structures\|Barnase 3D structures]]
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Clarke, J]]		[[Category: Clarke, J]]
	[[Category: Fersht, A R]]		[[Category: Fersht, A R]]
	[[Category: Henrick, K]]		[[Category: Henrick, K]]
	[[Category: Endonuclease]]		[[Category: Endonuclease]]

OCA at 06:06, 17 August 2017

OCA — Thu, 17 Aug 2017 06:06:48 GMT

←Older revision		Revision as of 06:06, 17 August 2017
Line 1:		Line 1:
		+
	==BARNASE A43C/S80C DISULFIDE MUTANT==		==BARNASE A43C/S80C DISULFIDE MUTANT==
	<StructureSection load='1bne' size='340' side='right' caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bne' size='340' side='right' caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BNE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BNE FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BARNASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [http://pdbe.org/1bne PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [http://www.ebi.ac.uk/pdbsum/1bne PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bne ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [http://pdbe.org/1bne PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [http://www.ebi.ac.uk/pdbsum/1bne PDBsum]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bne ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:57, 11 September 2015

OCA — Fri, 11 Sep 2015 07:57:19 GMT

←Older revision		Revision as of 07:57, 11 September 2015
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	<StructureSection load='1bne' size='340' side='right' caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bne' size='340' side='right' caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_amyloliquefaciens Bacillus amyloliquefaciens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BNE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BNE FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BARNASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 Bacillus ~~amyloliquefaciens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BARNASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 "Bacillus amyloliquifaciens" (sic) Fukumoto 1943])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [http://www.ebi.ac.uk/pdbsum/1bne PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [http://pdbe.org/1bne PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [http://www.ebi.ac.uk/pdbsum/1bne PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1bne" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Clarke, J]]		[[Category: Clarke, J]]
	[[Category: Fersht, A R]]		[[Category: Fersht, A R]]
	[[Category: Henrick, K]]		[[Category: Henrick, K]]
	[[Category: Endonuclease]]		[[Category: Endonuclease]]

OCA at 14:29, 15 February 2015

OCA — Sun, 15 Feb 2015 14:29:30 GMT

←Older revision		Revision as of 14:29, 15 February 2015
Line 31:		Line 31:
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
-	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:32, 25 December 2014

OCA — Thu, 25 Dec 2014 13:32:30 GMT

←Older revision		Revision as of 13:32, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [http://www.ebi.ac.uk/pdbsum/1bne PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [http://www.ebi.ac.uk/pdbsum/1bne PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM]] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:06, 22 December 2014

OCA — Mon, 22 Dec 2014 09:06:34 GMT

←Older revision		Revision as of 09:06, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BNE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BNE FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BARNASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 Bacillus amyloliquefaciens])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BARNASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 Bacillus amyloliquefaciens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [http://www.ebi.ac.uk/pdbsum/1bne PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [http://www.ebi.ac.uk/pdbsum/1bne PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	*[[Barnase\|Barnase]]		*[[Barnase\|Barnase]]
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
		+	*[[Temp\|Temp]]
		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
-	[[Category: Clarke, J.]]	+	[[Category: Clarke, J]]
-	[[Category: Fersht, A R.]]	+	[[Category: Fersht, A R]]
-	[[Category: Henrick, K.]]	+	[[Category: Henrick, K]]
	[[Category: Endonuclease]]		[[Category: Endonuclease]]

OCA at 09:39, 13 August 2014

OCA — Wed, 13 Aug 2014 09:39:29 GMT

←Older revision		Revision as of 09:39, 13 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1bne.~~png\|left~~\|200px]]	+	==BARNASE A43C/S80C DISULFIDE MUTANT==
		+	<StructureSection load='1bne' size='340' side='right' caption='[[1bne]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1bne]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BNE FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BARNASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1390 Bacillus amyloliquefaciens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bne OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bne RCSB], [http://www.ebi.ac.uk/pdbsum/1bne PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bn/1bne_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In this series of papers, we examine the effects of introducing disulfide bonds on the properties, structure and thermodynamics of a small globular protein, barnase. Three mutants have been made, in each of which a single crosslink confers different properties. Two of the disulfide bonds, between residues 43 and 80 (43-80) and between residues 85 and 102 (85-102), stabilise the protein, relative to both wild-type and the corresponding (reduced) dithiol forms: 85-102 is more stable than predicted from the entropic destabilisation of the unfolded state; 43-80 is less stable than predicted. The third disulfide bond, between residues 70 and 92 (70-92) destabilises the protein relative to both wild-type and the corresponding dithiol form, implying significant disruption of the folded protein on formation of the disulfide bond. Crystal structures of the three mutant proteins have been solved. All three proteins have essentially the same fold as wild-type, but with left-handed disulfide bonds, which have dihedral geometries that have not been observed in naturally occurring disulfides. In the very stable mutant 85-102, there is no significant difference between the mutant and wild-type structures: these data do not explain the large stability of this protein. The disulfide bond at 43-80 induces small structural rearrangements close to the site of the disulfide bond, associated with some local disorder: the crosslink appears to decrease the stability of the native form of the protein. The destabilising disulfide bond at 70-92 induces considerable structural change, with displacement of a loop and consequent disruption of a stabilising salt-bridge. Our studies do not support the view that the conformation of the disulfide bond is crucial in determining the stability of the mutant proteins.

-	~~{{STRUCTURE_1bne\| PDB=1bne \| SCENE= }}~~	+	Disulfide mutants of barnase. I: Changes in stability and structure assessed by biophysical methods and X-ray crystallography.,Clarke J, Henrick K, Fersht AR J Mol Biol. 1995 Oct 27;253(3):493-504. PMID:7473729<ref>PMID:7473729</ref>

-	~~===BARNASE A43C~~/~~S80C DISULFIDE MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7473729}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1bne]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA].~~	+

	==See Also==		==See Also==
	*[[Barnase\|Barnase]]		*[[Barnase\|Barnase]]
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:007473729<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
	[[Category: Clarke, J.]]		[[Category: Clarke, J.]]

OCA at 17:16, 20 October 2012

OCA — Sat, 20 Oct 2012 17:16:32 GMT

←Older revision		Revision as of 17:16, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1bne]] is a 3 chain structure ~~of [[Barnase]] and [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA].	+	[[1bne]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNE OCA].

	==See Also==		==See Also==