1bo6 - Revision history

OCA at 06:38, 7 February 2024

OCA — Wed, 07 Feb 2024 06:38:05 GMT

←Older revision		Revision as of 06:38, 7 February 2024
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	<StructureSection load='1bo6' size='340' side='right'caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bo6' size='340' side='right'caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=A3P:ADENOSINE~~-~~3-5-DIPHOSPHATE'>A3P</scene>~~, ~~<scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase]~~, ~~with EC number [https~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [https://pdbe.org/1bo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [https://www.ebi.ac.uk/pdbsum/1bo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bo6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [https://pdbe.org/1bo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [https://www.ebi.ac.uk/pdbsum/1bo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bo6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ST1E1_MOUSE ST1E1_MOUSE]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol.	+	[https://www.uniprot.org/uniprot/ST1E1_MOUSE ST1E1_MOUSE] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bo6 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bo6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Estrogen sulfotransferase (EST) catalyzes transfer of the 5'-sulfuryl group of adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to the 3alpha-phenol group of estrogenic steroids such as estradiol (E2). The recent crystal structure of EST-adenosine 3', 5'-diphosphate (PAP)- E2 complex has revealed that residues Lys48, Thr45, Thr51, Thr52, Lys106, His108, and Try240 are in position to play a catalytic role in the sulfuryl transfer reaction of EST (Kakuta Y., Pedersen, L. G., Carter, C. W., Negishi, M., and Pedersen, L. C. (1997) Nat. Struct. Biol. 4, 904-908). Mutation of Lys48, Lys106, or His108 nearly abolishes EST activity, indicating that they play a critical role in catalysis. A present 2.2-A resolution structure of EST-PAP-vanadate complex indicates that the vanadate molecule adopts a trigonal bipyramidal geometry with its equatorial oxygens coordinated to these three residues. The apical positions of the vanadate molecule are occupied by a terminal oxygen of the 5'-phosphate of PAP (2.1 A) and a possible water molecule (2. 3 A). This water molecule superimposes well to the 3alpha-phenol group of E2 in the crystal structure of the EST.PAP.E2 complex. These structures are characteristic of the transition state for an in-line sulfuryl transfer reaction from PAPS to E2. Moreover, residues Lys48, Lys106, and His108 are found to be coordinated with the vanadate molecule at the transition state of EST.
-
-	The sulfuryl transfer mechanism. Crystal structure of a vanadate complex of estrogen sulfotransferase and mutational analysis.,Kakuta Y, Petrotchenko EV, Pedersen LC, Negishi M J Biol Chem. 1998 Oct 16;273(42):27325-30. PMID:9765259<ref>PMID:9765259</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1bo6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Sulfotransferase\|Sulfotransferase]]	+	*[[Sulfotransferase 3D structures\|Sulfotransferase 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Estrone sulfotransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Kakuta, Y~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Negishi, M~~]]	+	[[Category: Kakuta Y]]
-	[[Category: ~~Pedersen, L C~~]]	+	[[Category: Negishi M]]
-	[[Category: ~~Pap]]~~	+	[[Category: Pedersen LC]]
-	~~[[Category: Sulfonation]]~~	+
-	~~[[Category: Sulfotransferase]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Vanadate~~]]	+

OCA at 07:01, 24 February 2021

OCA — Wed, 24 Feb 2021 07:01:19 GMT

←Older revision		Revision as of 07:01, 24 February 2021
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	<StructureSection load='1bo6' size='340' side='right'caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bo6' size='340' side='right'caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [~~http~~://pdbe.org/1bo6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1bo6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1bo6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [https://pdbe.org/1bo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [https://www.ebi.ac.uk/pdbsum/1bo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bo6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ST1E1_MOUSE ST1E1_MOUSE]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol.	+	[[https://www.uniprot.org/uniprot/ST1E1_MOUSE ST1E1_MOUSE]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:58, 21 May 2019

OCA — Tue, 21 May 2019 08:58:57 GMT

←Older revision		Revision as of 08:58, 21 May 2019
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	==ESTROGEN SULFOTRANSFERASE WITH INACTIVE COFACTOR PAP AND VANADATE==		==ESTROGEN SULFOTRANSFERASE WITH INACTIVE COFACTOR PAP AND VANADATE==
-	<StructureSection load='1bo6' size='340' side='right' caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1bo6' size='340' side='right'caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bo6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bo6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Estrone sulfotransferase]]		[[Category: Estrone sulfotransferase]]
		+	[[Category: Large Structures]]
	[[Category: Kakuta, Y]]		[[Category: Kakuta, Y]]
	[[Category: Negishi, M]]		[[Category: Negishi, M]]

OCA at 06:17, 17 August 2017

OCA — Thu, 17 Aug 2017 06:17:41 GMT

←Older revision		Revision as of 06:17, 17 August 2017
Line 1:		Line 1:
		+
	==ESTROGEN SULFOTRANSFERASE WITH INACTIVE COFACTOR PAP AND VANADATE==		==ESTROGEN SULFOTRANSFERASE WITH INACTIVE COFACTOR PAP AND VANADATE==
	<StructureSection load='1bo6' size='340' side='right' caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bo6' size='340' side='right' caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [http://pdbe.org/1bo6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1bo6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [http://pdbe.org/1bo6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1bo6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bo6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bo6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Estrone sulfotransferase]]		[[Category: Estrone sulfotransferase]]
-	[[Category: Lk3 transgenic mice]]
	[[Category: Kakuta, Y]]		[[Category: Kakuta, Y]]
	[[Category: Negishi, M]]		[[Category: Negishi, M]]

OCA at 16:49, 11 September 2015

OCA — Fri, 11 Sep 2015 16:49:08 GMT

←Older revision		Revision as of 16:49, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1bo6' size='340' side='right' caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bo6' size='340' side='right' caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1bo6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [http://pdbe.org/1bo6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1bo6 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1bo6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Estrone sulfotransferase]]		[[Category: Estrone sulfotransferase]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Kakuta, Y]]		[[Category: Kakuta, Y]]
	[[Category: Negishi, M]]		[[Category: Negishi, M]]

OCA at 19:13, 25 December 2014

OCA — Thu, 25 Dec 2014 19:13:44 GMT

←Older revision		Revision as of 19:13, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1bo6 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1bo6 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ST1E1_MOUSE ST1E1_MOUSE]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:53, 22 December 2014

OCA — Mon, 22 Dec 2014 08:53:02 GMT

←Older revision		Revision as of 08:53, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1bo6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1bo6 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Estrone sulfotransferase]]		[[Category: Estrone sulfotransferase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Kakuta, Y.]]	+	[[Category: Kakuta, Y]]
-	[[Category: Negishi, M.]]	+	[[Category: Negishi, M]]
-	[[Category: Pedersen, L C.]]	+	[[Category: Pedersen, L C]]
	[[Category: Pap]]		[[Category: Pap]]
	[[Category: Sulfonation]]		[[Category: Sulfonation]]

OCA at 09:48, 13 August 2014

OCA — Wed, 13 Aug 2014 09:48:12 GMT

←Older revision		Revision as of 09:48, 13 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1bo6.~~png\|left~~\|200px]]	+	==ESTROGEN SULFOTRANSFERASE WITH INACTIVE COFACTOR PAP AND VANADATE==
		+	<StructureSection load='1bo6' size='340' side='right' caption='[[1bo6]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1bo6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BO6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Estrone_sulfotransferase Estrone sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.4 2.8.2.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bo6 RCSB], [http://www.ebi.ac.uk/pdbsum/1bo6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bo6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Estrogen sulfotransferase (EST) catalyzes transfer of the 5'-sulfuryl group of adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to the 3alpha-phenol group of estrogenic steroids such as estradiol (E2). The recent crystal structure of EST-adenosine 3', 5'-diphosphate (PAP)- E2 complex has revealed that residues Lys48, Thr45, Thr51, Thr52, Lys106, His108, and Try240 are in position to play a catalytic role in the sulfuryl transfer reaction of EST (Kakuta Y., Pedersen, L. G., Carter, C. W., Negishi, M., and Pedersen, L. C. (1997) Nat. Struct. Biol. 4, 904-908). Mutation of Lys48, Lys106, or His108 nearly abolishes EST activity, indicating that they play a critical role in catalysis. A present 2.2-A resolution structure of EST-PAP-vanadate complex indicates that the vanadate molecule adopts a trigonal bipyramidal geometry with its equatorial oxygens coordinated to these three residues. The apical positions of the vanadate molecule are occupied by a terminal oxygen of the 5'-phosphate of PAP (2.1 A) and a possible water molecule (2. 3 A). This water molecule superimposes well to the 3alpha-phenol group of E2 in the crystal structure of the EST.PAP.E2 complex. These structures are characteristic of the transition state for an in-line sulfuryl transfer reaction from PAPS to E2. Moreover, residues Lys48, Lys106, and His108 are found to be coordinated with the vanadate molecule at the transition state of EST.

-	~~{{STRUCTURE_1bo6\| PDB=1bo6 \| SCENE= }}~~	+	The sulfuryl transfer mechanism. Crystal structure of a vanadate complex of estrogen sulfotransferase and mutational analysis.,Kakuta Y, Petrotchenko EV, Pedersen LC, Negishi M J Biol Chem. 1998 Oct 16;273(42):27325-30. PMID:9765259<ref>PMID:9765259</ref>

-	~~===ESTROGEN SULFOTRANSFERASE WITH INACTIVE COFACTOR PAP AND VANADATE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9765259}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1bo6]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA].~~	+

	==See Also==		==See Also==
	*[[Sulfotransferase\|Sulfotransferase]]		*[[Sulfotransferase\|Sulfotransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009765259<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Estrone sulfotransferase]]		[[Category: Estrone sulfotransferase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 20:25, 20 October 2012

OCA — Sat, 20 Oct 2012 20:25:37 GMT

←Older revision		Revision as of 20:25, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1bo6]] is a 2 chain structure ~~of [[Sulfotransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA].	+	[[1bo6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BO6 OCA].

	==See Also==		==See Also==

OCA: Protected "1bo6" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 10:27:30 GMT

Protected "1bo6" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:27, 27 July 2012