1bq7 - Revision history

OCA at 05:40, 9 August 2023

OCA — Wed, 09 Aug 2023 05:40:48 GMT

←Older revision		Revision as of 05:40, 9 August 2023
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	<StructureSection load='1bq7' size='340' side='right'caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1bq7' size='340' side='right'caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [https://pdbe.org/1bq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [https://www.ebi.ac.uk/pdbsum/1bq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bq7 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [https://pdbe.org/1bq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [https://www.ebi.ac.uk/pdbsum/1bq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bq7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DSBA_ECOLI DSBA_ECOLI]] Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.<ref>PMID:1429594</ref> <ref>PMID:22267510</ref>	+	[https://www.uniprot.org/uniprot/DSBA_ECOLI DSBA_ECOLI] Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.<ref>PMID:1429594</ref> <ref>PMID:22267510</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Charbonnier, J B]]	+	[[Category: Charbonnier J-B]]
-	[[Category: Stura~~, E A]]~~	+	[[Category: Stura EA]]
-	~~[[Category: Disulfide oxidoreductase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Protein disulfide isomerase]]~~	+
-	~~[[Category: Protein folding]]~~	+
-	~~[[Category: Redox protein]]~~	+
-	~~[[Category: Redox-active center~~]]	+

OCA at 14:47, 3 November 2021

OCA — Wed, 03 Nov 2021 14:47:54 GMT

←Older revision		Revision as of 14:47, 3 November 2021
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	<StructureSection load='1bq7' size='340' side='right'caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1bq7' size='340' side='right'caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [https://pdbe.org/1bq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [https://www.ebi.ac.uk/pdbsum/1bq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bq7 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [https://pdbe.org/1bq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [https://www.ebi.ac.uk/pdbsum/1bq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bq7 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Charbonnier, J B]]		[[Category: Charbonnier, J B]]

OCA at 07:01, 24 February 2021

OCA — Wed, 24 Feb 2021 07:01:41 GMT

←Older revision		Revision as of 07:01, 24 February 2021
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	<StructureSection load='1bq7' size='340' side='right'caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1bq7' size='340' side='right'caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [~~http~~://pdbe.org/1bq7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1bq7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1bq7 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [https://pdbe.org/1bq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [https://www.ebi.ac.uk/pdbsum/1bq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bq7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DSBA_ECOLI DSBA_ECOLI]] Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.<ref>PMID:1429594</ref> <ref>PMID:22267510</ref>	+	[[https://www.uniprot.org/uniprot/DSBA_ECOLI DSBA_ECOLI]] Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.<ref>PMID:1429594</ref> <ref>PMID:22267510</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:05, 21 May 2019

OCA — Tue, 21 May 2019 09:05:10 GMT

←Older revision		Revision as of 09:05, 21 May 2019
Line 1:		Line 1:

	==DSBA MUTANT P151A, ROLE OF THE CIS-PROLINE IN THE ACTIVE SITE OF DSBA==		==DSBA MUTANT P151A, ROLE OF THE CIS-PROLINE IN THE ACTIVE SITE OF DSBA==
-	<StructureSection load='1bq7' size='340' side='right' caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='1bq7' size='340' side='right'caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bq/1bq7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bq/1bq7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Charbonnier, J B]]		[[Category: Charbonnier, J B]]
	[[Category: Stura, E A]]		[[Category: Stura, E A]]

OCA at 06:02, 17 August 2017

OCA — Thu, 17 Aug 2017 06:02:27 GMT

←Older revision		Revision as of 06:02, 17 August 2017
Line 1:		Line 1:
		+
	==DSBA MUTANT P151A, ROLE OF THE CIS-PROLINE IN THE ACTIVE SITE OF DSBA==		==DSBA MUTANT P151A, ROLE OF THE CIS-PROLINE IN THE ACTIVE SITE OF DSBA==
	<StructureSection load='1bq7' size='340' side='right' caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1bq7' size='340' side='right' caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [http://pdbe.org/1bq7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [http://www.ebi.ac.uk/pdbsum/1bq7 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [http://pdbe.org/1bq7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [http://www.ebi.ac.uk/pdbsum/1bq7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bq7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bq7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus coli migula 1895]]
	[[Category: Charbonnier, J B]]		[[Category: Charbonnier, J B]]
	[[Category: Stura, E A]]		[[Category: Stura, E A]]

OCA at 09:19, 11 September 2015

OCA — Fri, 11 Sep 2015 09:19:27 GMT

←Older revision		Revision as of 09:19, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1bq7' size='340' side='right' caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1bq7' size='340' side='right' caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [http://www.ebi.ac.uk/pdbsum/1bq7 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [http://pdbe.org/1bq7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [http://www.ebi.ac.uk/pdbsum/1bq7 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1bq7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Charbonnier, J B]]		[[Category: Charbonnier, J B]]
	[[Category: Stura, E A]]		[[Category: Stura, E A]]

OCA at 14:25, 25 December 2014

OCA — Thu, 25 Dec 2014 14:25:05 GMT

←Older revision		Revision as of 14:25, 25 December 2014
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [http://www.ebi.ac.uk/pdbsum/1bq7 PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [http://www.ebi.ac.uk/pdbsum/1bq7 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DSBA_ECOLI DSBA_ECOLI]] Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.<ref>PMID:1429594</ref> <ref>PMID:22267510</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:59, 22 December 2014

OCA — Mon, 22 Dec 2014 10:59:21 GMT

←Older revision		Revision as of 10:59, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [http://www.ebi.ac.uk/pdbsum/1bq7 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [http://www.ebi.ac.uk/pdbsum/1bq7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Charbonnier, J B.]]	+	[[Category: Charbonnier, J B]]
-	[[Category: Stura, E A.]]	+	[[Category: Stura, E A]]
	[[Category: Disulfide oxidoreductase]]		[[Category: Disulfide oxidoreductase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 09:35, 13 August 2014

OCA — Wed, 13 Aug 2014 09:35:14 GMT

←Older revision		Revision as of 09:35, 13 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1bq7.~~png\|left~~\|200px]]	+	==DSBA MUTANT P151A, ROLE OF THE CIS-PROLINE IN THE ACTIVE SITE OF DSBA==
		+	<StructureSection load='1bq7' size='340' side='right' caption='[[1bq7]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1bq7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BQ7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bq7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bq7 RCSB], [http://www.ebi.ac.uk/pdbsum/1bq7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bq/1bq7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In addition to the Cys-Xaa-Xaa-Cys motif at position 30-33, DsbA, the essential catalyst for disulfide bond formation in the bacterial periplasm shares with other oxidoreductases of the thioredoxin family a cis-proline in proximity of the active site residues. In the variant DsbA(P151A), this residue has been changed to an alanine, an almost isosteric residue which is not disposed to adopt the cis conformation. The substitution strongly destabilized the structure of DsbA, as determined by the decrease in the free energy of folding. The pKa of the thiol of Cys30 was only marginally decreased. Although in vivo the variant appeared to be correctly oxidized, it exhibited an activity less than half that of the wild-type enzyme with respect to the folding of alkaline phosphatase, used as a reporter of the disulfide bond formation in the periplasm. DsbA(P151A) crystallized in a different crystal form from the wild-type protein, in space group P2(1) with six molecules in the asymmetric unit. Its X-ray structure was determined to 2.8 A resolution. The most significant conformational changes occurred at the active site. The loop 149-152 adopted a new backbone conformation with Ala151 in a trans conformation. This rearrangement resulted in the loss of van der Waals interactions between this loop and the disulfide bond. His32 from the Cys-Xaa-Xaa-Cys sequence presented in four out of six molecules in the asymmetric unit a gauche conformation not observed in the wild-type protein. The X-ray structure and folding studies on DsbA(P151A) were consistent with the cis-proline playing a major role in the stabilization of the protein. A role for the positioning of the substrate is discussed. These important properties for the enzyme function might explain the conservation of this residue in DsbA and related proteins possessing the thioredoxin fold.

-	~~{{STRUCTURE_1bq7\| PDB=1bq7 \| SCENE= }}~~	+	On the role of the cis-proline residue in the active site of DsbA.,Charbonnier JB, Belin P, Moutiez M, Stura EA, Quemeneur E Protein Sci. 1999 Jan;8(1):96-105. PMID:10210188<ref>PMID:10210188</ref>

-	~~===DSBA MUTANT P151A~~, ~~ROLE OF THE CIS-PROLINE IN THE ACTIVE SITE OF DSBA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10210188}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1bq7]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA].~~	+

	==See Also==		==See Also==
	*[[Protein disulfide oxidoreductase\|Protein disulfide oxidoreductase]]		*[[Protein disulfide oxidoreductase\|Protein disulfide oxidoreductase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010210188<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Charbonnier, J B.]]		[[Category: Charbonnier, J B.]]

OCA at 17:47, 20 October 2012

OCA — Sat, 20 Oct 2012 17:47:48 GMT

←Older revision		Revision as of 17:47, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1bq7]] is a 6 chain structure ~~of [[Protein disulfide oxidoreductase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA].	+	[[1bq7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQ7 OCA].

	==See Also==		==See Also==