1cbg - Revision history

OCA at 15:38, 13 March 2024

OCA — Wed, 13 Mar 2024 15:38:48 GMT

←Older revision		Revision as of 15:38, 13 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CBG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CBG FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>~~[https://en.wikipedia.org/wiki/Beta~~-~~glucosidase Beta-glucosidase]~~, ~~with EC number~~ [~~https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.~~2.~~1.21 3.2.1.21] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [https://pdbe.org/1cbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [https://www.ebi.ac.uk/pdbsum/1cbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cbg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [https://pdbe.org/1cbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [https://www.ebi.ac.uk/pdbsum/1cbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cbg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BGLT_TRIRP BGLT_TRIRP]] Hydrolyzes cyanoglucosides, contributing to the release of hydrocyanic acid, which functions as a defense mechanism against small predators, when the leaf tissue is damaged.	+	[https://www.uniprot.org/uniprot/BGLT_TRIRP BGLT_TRIRP] Hydrolyzes cyanoglucosides, contributing to the release of hydrocyanic acid, which functions as a defense mechanism against small predators, when the leaf tissue is damaged.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cbg ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cbg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: beta-glucosidases occur in a variety of organisms and catalyze the hydrolysis of aryl and alkyl-beta-D-glucosides as well as glucosides with only a carbohydrate moiety (such as cellobiose). The cyanogenic beta-glucosidase from white clover (subsequently referred to as CBG) is responsible for the cleavage of cyanoglucosides. Both CBG and the cyanoglucosides occur within the plant cell wall where they are found in separate compartments and only come into contact when the leaf tissue experiences mechanical damage. This results in the eventual production of hydrogen cyanide which acts as a deterrent to grazing animals. beta-glucosidases have been assigned to particular glycosyl hydrolase families on the basis of sequence similarity; this classification has placed CBG in family 1 (there are a total of over 40 families) for which a three-dimensional structure has so far not been determined. This is the first report of the three-dimensional structure of a glycosyl hydrolase from family 1. RESULTS: The crystal structure of CBG has been determined using multiple isomorphous replacement. The final model has been refined at 2.15 A resolution to an R factor of 18.9%. The overall fold of the molecule is a (beta/alpha)8 [or (alpha/beta)8] barrel (in common with a number of glycosyl hydrolases) with all residues located in a single domain. CONCLUSIONS: Sequence comparisons between beta-glucosidases of the same family show that residues Glu183 and Glu397 are highly conserved. Both residues are positioned at the end of a pocket located at the C terminus of the barrel and have been assigned the respective roles of proton donor and nucleophile on the basis of inhibitor-binding and mutagenesis experiments. These roles are consistent with the environments of the two residues. The pocket itself is typical of a sugar-binding site as it contains a number of charged, aromatic and polar groups. In support of this role, we present crystallographic data on a possible product complex between CBG and glucose, resulting from co-crystallization of the native enzyme with its natural substrate, linamarin.
-
-	The crystal structure of a cyanogenic beta-glucosidase from white clover, a family 1 glycosyl hydrolase.,Barrett T, Suresh CG, Tolley SP, Dodson EJ, Hughes MA Structure. 1995 Sep 15;3(9):951-60. PMID:8535788<ref>PMID:8535788</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1cbg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]		*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-glucosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Trifolium repens]]		[[Category: Trifolium repens]]
-	[[Category: Barrett~~, T E~~]]	+	[[Category: Barrett TE]]
-	[[Category: Hughes~~, M A~~]]	+	[[Category: Hughes MA]]
-	[[Category: Suresh~~, C G~~]]	+	[[Category: Suresh CG]]
-	[[Category: Tolley~~, S P]]~~	+	[[Category: Tolley SP]]
-	~~[[Category: Cyanogenic beta-glucosidase~~]]	+

OCA at 10:43, 19 May 2021

OCA — Wed, 19 May 2021 10:43:08 GMT

←Older revision		Revision as of 10:43, 19 May 2021
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	<StructureSection load='1cbg' size='340' side='right'caption='[[1cbg]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='1cbg' size='340' side='right'caption='[[1cbg]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CBG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CBG FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [~~http~~://pdbe.org/1cbg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cbg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cbg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [https://pdbe.org/1cbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [https://www.ebi.ac.uk/pdbsum/1cbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cbg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BGLT_TRIRP BGLT_TRIRP]] Hydrolyzes cyanoglucosides, contributing to the release of hydrocyanic acid, which functions as a defense mechanism against small predators, when the leaf tissue is damaged.	+	[[https://www.uniprot.org/uniprot/BGLT_TRIRP BGLT_TRIRP]] Hydrolyzes cyanoglucosides, contributing to the release of hydrocyanic acid, which functions as a defense mechanism against small predators, when the leaf tissue is damaged.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:54, 17 July 2019

OCA — Wed, 17 Jul 2019 10:54:52 GMT

←Older revision		Revision as of 10:54, 17 July 2019
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	==THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE==		==THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE==
-	<StructureSection load='1cbg' size='340' side='right' caption='[[1cbg]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='1cbg' size='340' side='right'caption='[[1cbg]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CBG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CBG FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/1cbg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/1cbg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1cbg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cbg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
		+	[[Category: Large Structures]]
	[[Category: Trifolium repens]]		[[Category: Trifolium repens]]
	[[Category: Barrett, T E]]		[[Category: Barrett, T E]]

OCA at 08:07, 29 November 2017

OCA — Wed, 29 Nov 2017 08:07:18 GMT

←Older revision		Revision as of 08:07, 29 November 2017
Line 1:		Line 1:
		+
	==THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE==		==THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE==
	<StructureSection load='1cbg' size='340' side='right' caption='[[1cbg]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='1cbg' size='340' side='right' caption='[[1cbg]], [[Resolution\|resolution]] 2.15Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CBG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CBG FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [http://pdbe.org/1cbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [http://www.ebi.ac.uk/pdbsum/1cbg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [http://pdbe.org/1cbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [http://www.ebi.ac.uk/pdbsum/1cbg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cbg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1cbg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cbg" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Beta-glucosidase\|Beta-glucosidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:58, 8 February 2016

OCA — Mon, 08 Feb 2016 08:58:35 GMT

←Older revision		Revision as of 08:58, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cbg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:57, 10 September 2015

OCA — Thu, 10 Sep 2015 09:57:51 GMT

←Older revision		Revision as of 09:57, 10 September 2015
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	<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CBG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CBG FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [http://www.ebi.ac.uk/pdbsum/1cbg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [http://pdbe.org/1cbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [http://www.ebi.ac.uk/pdbsum/1cbg PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cbg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 22:07, 24 December 2014

OCA — Wed, 24 Dec 2014 22:07:17 GMT

←Older revision		Revision as of 22:07, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [http://www.ebi.ac.uk/pdbsum/1cbg PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [http://www.ebi.ac.uk/pdbsum/1cbg PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BGLT_TRIRP BGLT_TRIRP]] Hydrolyzes cyanoglucosides, contributing to the release of hydrocyanic acid, which functions as a defense mechanism against small predators, when the leaf tissue is damaged.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:33, 22 December 2014

OCA — Mon, 22 Dec 2014 08:33:22 GMT

←Older revision		Revision as of 08:33, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CBG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CBG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [http://www.ebi.ac.uk/pdbsum/1cbg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [http://www.ebi.ac.uk/pdbsum/1cbg PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
	[[Category: Trifolium repens]]		[[Category: Trifolium repens]]
-	[[Category: Barrett, T E.]]	+	[[Category: Barrett, T E]]
-	[[Category: Hughes, M A.]]	+	[[Category: Hughes, M A]]
-	[[Category: Suresh, C G.]]	+	[[Category: Suresh, C G]]
-	[[Category: Tolley, S P.]]	+	[[Category: Tolley, S P]]
	[[Category: Cyanogenic beta-glucosidase]]		[[Category: Cyanogenic beta-glucosidase]]

OCA at 16:59, 20 August 2014

OCA — Wed, 20 Aug 2014 16:59:30 GMT

←Older revision		Revision as of 16:59, 20 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1cbg.~~png\|left~~\|200px]]	+	==THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE==
		+	<StructureSection load='1cbg' size='340' side='right' caption='[[1cbg]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CBG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cbg RCSB], [http://www.ebi.ac.uk/pdbsum/1cbg PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/1cbg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: beta-glucosidases occur in a variety of organisms and catalyze the hydrolysis of aryl and alkyl-beta-D-glucosides as well as glucosides with only a carbohydrate moiety (such as cellobiose). The cyanogenic beta-glucosidase from white clover (subsequently referred to as CBG) is responsible for the cleavage of cyanoglucosides. Both CBG and the cyanoglucosides occur within the plant cell wall where they are found in separate compartments and only come into contact when the leaf tissue experiences mechanical damage. This results in the eventual production of hydrogen cyanide which acts as a deterrent to grazing animals. beta-glucosidases have been assigned to particular glycosyl hydrolase families on the basis of sequence similarity; this classification has placed CBG in family 1 (there are a total of over 40 families) for which a three-dimensional structure has so far not been determined. This is the first report of the three-dimensional structure of a glycosyl hydrolase from family 1. RESULTS: The crystal structure of CBG has been determined using multiple isomorphous replacement. The final model has been refined at 2.15 A resolution to an R factor of 18.9%. The overall fold of the molecule is a (beta/alpha)8 [or (alpha/beta)8] barrel (in common with a number of glycosyl hydrolases) with all residues located in a single domain. CONCLUSIONS: Sequence comparisons between beta-glucosidases of the same family show that residues Glu183 and Glu397 are highly conserved. Both residues are positioned at the end of a pocket located at the C terminus of the barrel and have been assigned the respective roles of proton donor and nucleophile on the basis of inhibitor-binding and mutagenesis experiments. These roles are consistent with the environments of the two residues. The pocket itself is typical of a sugar-binding site as it contains a number of charged, aromatic and polar groups. In support of this role, we present crystallographic data on a possible product complex between CBG and glucose, resulting from co-crystallization of the native enzyme with its natural substrate, linamarin.

-	~~{{STRUCTURE_1cbg\| PDB=1cbg \| SCENE= }}~~	+	The crystal structure of a cyanogenic beta-glucosidase from white clover, a family 1 glycosyl hydrolase.,Barrett T, Suresh CG, Tolley SP, Dodson EJ, Hughes MA Structure. 1995 Sep 15;3(9):951-60. PMID:8535788<ref>PMID:8535788</ref>

-	~~===THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.)~~, ~~A FAMILY 1 GLYCOSYL-HYDROLASE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8535788}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1cbg]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Trifolium_repens Trifolium repens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA].~~	+

	==See Also==		==See Also==
	*[[Beta-glucosidase\|Beta-glucosidase]]		*[[Beta-glucosidase\|Beta-glucosidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:008535788<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
	[[Category: Trifolium repens]]		[[Category: Trifolium repens]]

OCA at 09:37, 20 October 2012

OCA — Sat, 20 Oct 2012 09:37:35 GMT

←Older revision		Revision as of 09:37, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1cbg]] is a 1 chain structure ~~of [[Beta-glucosidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA].	+	[[1cbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trifolium_repens Trifolium repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBG OCA].

	==See Also==		==See Also==